Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS12870 and DZA65_RS12875 are separated by 55 nucleotides DZA65_RS12875 and DZA65_RS12880 are separated by 123 nucleotides DZA65_RS12880 and DZA65_RS12885 are separated by 134 nucleotides
DZA65_RS12870: DZA65_RS12870 - murein tripeptide amidase MpaA, at 2,758,550 to 2,759,281
_RS12870
DZA65_RS12875: DZA65_RS12875 - thiol peroxidase, at 2,759,337 to 2,759,840
_RS12875
DZA65_RS12880: DZA65_RS12880 - MFS transporter, at 2,759,964 to 2,761,376
_RS12880
DZA65_RS12885: DZA65_RS12885 - helix-turn-helix domain-containing protein, at 2,761,511 to 2,762,410
_RS12885
Position (kb)
2759
2760
2761
2762 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2759.229 kb on - strand at 2759.270 kb on + strand at 2759.271 kb on - strand at 2759.310 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.332 kb on + strand at 2759.333 kb on - strand at 2759.333 kb on - strand at 2759.333 kb on - strand at 2759.333 kb on - strand at 2759.333 kb on - strand at 2759.378 kb on - strand at 2759.463 kb on + strand, within DZA65_RS12875 at 2759.489 kb on + strand, within DZA65_RS12875 at 2759.535 kb on - strand, within DZA65_RS12875 at 2759.564 kb on + strand, within DZA65_RS12875 at 2759.567 kb on + strand, within DZA65_RS12875 at 2759.567 kb on + strand, within DZA65_RS12875 at 2759.568 kb on - strand, within DZA65_RS12875 at 2759.568 kb on - strand, within DZA65_RS12875 at 2759.689 kb on - strand, within DZA65_RS12875 at 2759.772 kb on + strand, within DZA65_RS12875 at 2759.772 kb on + strand, within DZA65_RS12875 at 2759.772 kb on + strand, within DZA65_RS12875 at 2759.772 kb on + strand, within DZA65_RS12875 at 2759.772 kb on + strand, within DZA65_RS12875 at 2759.773 kb on - strand, within DZA65_RS12875 at 2759.811 kb on + strand at 2759.811 kb on + strand at 2759.811 kb on + strand at 2759.812 kb on - strand at 2759.812 kb on - strand at 2759.838 kb on + strand at 2759.839 kb on - strand at 2759.839 kb on - strand at 2759.864 kb on - strand at 2759.869 kb on + strand at 2759.869 kb on + strand at 2759.869 kb on + strand at 2759.869 kb on + strand at 2759.869 kb on + strand at 2759.869 kb on + strand at 2759.870 kb on - strand at 2759.870 kb on - strand at 2759.870 kb on - strand at 2759.870 kb on - strand at 2759.870 kb on - strand at 2759.888 kb on - strand at 2759.888 kb on - strand at 2759.899 kb on + strand at 2759.932 kb on - strand at 2759.932 kb on - strand at 2759.932 kb on - strand at 2759.935 kb on + strand at 2759.935 kb on + strand at 2759.935 kb on + strand at 2759.936 kb on - strand at 2759.936 kb on - strand at 2759.936 kb on - strand at 2759.936 kb on - strand at 2759.936 kb on - strand at 2759.985 kb on + strand at 2759.986 kb on - strand at 2760.015 kb on + strand at 2760.025 kb on - strand at 2760.105 kb on + strand at 2760.105 kb on + strand at 2760.223 kb on + strand, within DZA65_RS12880 at 2760.239 kb on + strand, within DZA65_RS12880 at 2760.239 kb on + strand, within DZA65_RS12880 at 2760.239 kb on + strand, within DZA65_RS12880 at 2760.428 kb on - strand, within DZA65_RS12880 at 2760.428 kb on - strand, within DZA65_RS12880 at 2760.428 kb on - strand, within DZA65_RS12880 at 2760.428 kb on - strand, within DZA65_RS12880 at 2760.474 kb on - strand, within DZA65_RS12880 at 2760.499 kb on + strand, within DZA65_RS12880 at 2760.499 kb on + strand, within DZA65_RS12880 at 2760.500 kb on - strand, within DZA65_RS12880 at 2760.500 kb on - strand, within DZA65_RS12880 at 2760.500 kb on - strand, within DZA65_RS12880 at 2760.515 kb on + strand, within DZA65_RS12880 at 2760.515 kb on + strand, within DZA65_RS12880 at 2760.515 kb on + strand, within DZA65_RS12880 at 2760.516 kb on - strand, within DZA65_RS12880 at 2760.516 kb on - strand, within DZA65_RS12880 at 2760.516 kb on - strand, within DZA65_RS12880 at 2760.600 kb on + strand, within DZA65_RS12880 at 2760.723 kb on + strand, within DZA65_RS12880 at 2760.723 kb on + strand, within DZA65_RS12880 at 2760.723 kb on + strand, within DZA65_RS12880 at 2760.723 kb on + strand, within DZA65_RS12880 at 2760.724 kb on - strand, within DZA65_RS12880 at 2760.724 kb on - strand, within DZA65_RS12880 at 2760.764 kb on + strand, within DZA65_RS12880 at 2760.765 kb on - strand, within DZA65_RS12880 at 2760.952 kb on + strand, within DZA65_RS12880 at 2761.207 kb on + strand, within DZA65_RS12880 at 2761.207 kb on + strand, within DZA65_RS12880 at 2761.207 kb on + strand, within DZA65_RS12880 at 2761.208 kb on - strand, within DZA65_RS12880 at 2761.208 kb on - strand, within DZA65_RS12880 at 2761.208 kb on - strand, within DZA65_RS12880 at 2761.382 kb on + strand at 2761.383 kb on - strand at 2761.394 kb on + strand at 2761.411 kb on + strand at 2761.412 kb on - strand at 2761.412 kb on - strand at 2761.429 kb on - strand at 2761.459 kb on - strand at 2761.467 kb on + strand at 2761.468 kb on - strand at 2761.468 kb on - strand at 2761.468 kb on - strand at 2761.468 kb on - strand at 2761.510 kb on + strand at 2761.552 kb on + strand at 2761.560 kb on + strand at 2761.561 kb on - strand at 2761.616 kb on - strand, within DZA65_RS12885 at 2761.616 kb on - strand, within DZA65_RS12885 at 2761.616 kb on - strand, within DZA65_RS12885 at 2761.616 kb on - strand, within DZA65_RS12885 at 2761.672 kb on - strand, within DZA65_RS12885 at 2761.711 kb on + strand, within DZA65_RS12885 at 2761.711 kb on + strand, within DZA65_RS12885 at 2761.711 kb on + strand, within DZA65_RS12885 at 2761.711 kb on + strand, within DZA65_RS12885 at 2761.711 kb on + strand, within DZA65_RS12885 at 2761.712 kb on - strand, within DZA65_RS12885 at 2761.718 kb on + strand, within DZA65_RS12885 at 2761.718 kb on + strand, within DZA65_RS12885 at 2761.719 kb on - strand, within DZA65_RS12885 at 2761.719 kb on - strand, within DZA65_RS12885 at 2761.719 kb on - strand, within DZA65_RS12885 at 2761.822 kb on + strand, within DZA65_RS12885 at 2761.823 kb on - strand, within DZA65_RS12885 at 2761.894 kb on + strand, within DZA65_RS12885 at 2761.895 kb on - strand, within DZA65_RS12885 at 2761.895 kb on - strand, within DZA65_RS12885 at 2761.895 kb on - strand, within DZA65_RS12885 at 2761.895 kb on - strand, within DZA65_RS12885 at 2761.950 kb on + strand, within DZA65_RS12885 at 2761.950 kb on + strand, within DZA65_RS12885 at 2761.951 kb on - strand, within DZA65_RS12885 at 2761.951 kb on - strand, within DZA65_RS12885 at 2762.026 kb on + strand, within DZA65_RS12885 at 2762.026 kb on + strand, within DZA65_RS12885 at 2762.027 kb on - strand, within DZA65_RS12885 at 2762.027 kb on - strand at 2762.242 kb on + strand, within DZA65_RS12885 at 2762.242 kb on + strand, within DZA65_RS12885 at 2762.242 kb on + strand, within DZA65_RS12885 at 2762.270 kb on - strand, within DZA65_RS12885 at 2762.270 kb on - strand, within DZA65_RS12885 at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.333 kb on + strand at 2762.334 kb on - strand at 2762.334 kb on - strand at 2762.334 kb on - strand at 2762.334 kb on - strand at 2762.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 2,759,229 - -1.1 2,759,270 + -1.9 2,759,271 - +1.3 2,759,310 + -0.0 2,759,332 + -0.3 2,759,332 + -0.8 2,759,332 + +2.3 2,759,332 + -0.9 2,759,332 + +2.6 2,759,332 + -0.1 2,759,332 + +0.8 2,759,332 + +0.2 2,759,332 + +0.7 2,759,332 + -1.6 2,759,332 + -1.1 2,759,332 + +0.3 2,759,332 + -1.3 2,759,333 - +0.2 2,759,333 - +1.3 2,759,333 - -1.9 2,759,333 - -1.6 2,759,333 - +1.7 2,759,378 - +2.0 2,759,463 + DZA65_RS12875 0.25 +0.7 2,759,489 + DZA65_RS12875 0.30 -1.3 2,759,535 - DZA65_RS12875 0.39 -1.6 2,759,564 + DZA65_RS12875 0.45 +0.7 2,759,567 + DZA65_RS12875 0.46 -0.3 2,759,567 + DZA65_RS12875 0.46 -1.6 2,759,568 - DZA65_RS12875 0.46 +0.7 2,759,568 - DZA65_RS12875 0.46 +2.3 2,759,689 - DZA65_RS12875 0.70 -3.0 2,759,772 + DZA65_RS12875 0.86 -0.6 2,759,772 + DZA65_RS12875 0.86 +1.3 2,759,772 + DZA65_RS12875 0.86 +0.5 2,759,772 + DZA65_RS12875 0.86 -1.6 2,759,772 + DZA65_RS12875 0.86 -0.3 2,759,773 - DZA65_RS12875 0.87 +1.7 2,759,811 + -2.5 2,759,811 + -0.6 2,759,811 + -0.9 2,759,812 - -1.4 2,759,812 - -0.1 2,759,838 + -2.1 2,759,839 - -1.3 2,759,839 - -1.3 2,759,864 - -2.8 2,759,869 + +2.0 2,759,869 + +0.8 2,759,869 + +0.7 2,759,869 + -2.1 2,759,869 + +1.0 2,759,869 + -0.9 2,759,870 - +0.7 2,759,870 - -0.1 2,759,870 - -1.1 2,759,870 - -0.8 2,759,870 - -4.2 2,759,888 - -1.3 2,759,888 - -1.3 2,759,899 + -3.0 2,759,932 - +1.3 2,759,932 - +0.0 2,759,932 - +0.1 2,759,935 + +0.0 2,759,935 + -1.0 2,759,935 + -1.3 2,759,936 - -2.1 2,759,936 - -3.9 2,759,936 - +1.1 2,759,936 - +1.4 2,759,936 - -0.6 2,759,985 + -0.5 2,759,986 - -2.1 2,760,015 + -2.6 2,760,025 - +2.3 2,760,105 + -1.6 2,760,105 + -2.5 2,760,223 + DZA65_RS12880 0.18 -0.3 2,760,239 + DZA65_RS12880 0.19 +0.7 2,760,239 + DZA65_RS12880 0.19 -2.1 2,760,239 + DZA65_RS12880 0.19 +2.3 2,760,428 - DZA65_RS12880 0.33 +0.9 2,760,428 - DZA65_RS12880 0.33 -1.6 2,760,428 - DZA65_RS12880 0.33 +0.3 2,760,428 - DZA65_RS12880 0.33 -1.9 2,760,474 - DZA65_RS12880 0.36 -3.8 2,760,499 + DZA65_RS12880 0.38 -0.6 2,760,499 + DZA65_RS12880 0.38 +1.7 2,760,500 - DZA65_RS12880 0.38 -1.3 2,760,500 - DZA65_RS12880 0.38 -1.5 2,760,500 - DZA65_RS12880 0.38 +1.6 2,760,515 + DZA65_RS12880 0.39 -0.5 2,760,515 + DZA65_RS12880 0.39 -1.3 2,760,515 + DZA65_RS12880 0.39 -0.5 2,760,516 - DZA65_RS12880 0.39 -2.8 2,760,516 - DZA65_RS12880 0.39 -2.3 2,760,516 - DZA65_RS12880 0.39 -2.1 2,760,600 + DZA65_RS12880 0.45 -0.6 2,760,723 + DZA65_RS12880 0.54 -0.3 2,760,723 + DZA65_RS12880 0.54 -2.1 2,760,723 + DZA65_RS12880 0.54 -2.8 2,760,723 + DZA65_RS12880 0.54 +1.2 2,760,724 - DZA65_RS12880 0.54 -0.9 2,760,724 - DZA65_RS12880 0.54 -1.9 2,760,764 + DZA65_RS12880 0.57 +0.7 2,760,765 - DZA65_RS12880 0.57 +1.0 2,760,952 + DZA65_RS12880 0.70 +1.0 2,761,207 + DZA65_RS12880 0.88 -2.6 2,761,207 + DZA65_RS12880 0.88 +0.2 2,761,207 + DZA65_RS12880 0.88 -0.5 2,761,208 - DZA65_RS12880 0.88 -0.3 2,761,208 - DZA65_RS12880 0.88 -0.3 2,761,208 - DZA65_RS12880 0.88 -1.3 2,761,382 + -0.8 2,761,383 - +0.4 2,761,394 + +1.6 2,761,411 + -1.1 2,761,412 - -0.6 2,761,412 - -1.2 2,761,429 - +0.2 2,761,459 - -1.6 2,761,467 + +0.7 2,761,468 - -2.3 2,761,468 - +0.1 2,761,468 - +0.1 2,761,468 - -1.9 2,761,510 + +1.0 2,761,552 + +0.0 2,761,560 + -1.6 2,761,561 - -1.6 2,761,616 - DZA65_RS12885 0.12 -0.4 2,761,616 - DZA65_RS12885 0.12 +0.0 2,761,616 - DZA65_RS12885 0.12 -1.6 2,761,616 - DZA65_RS12885 0.12 +3.6 2,761,672 - DZA65_RS12885 0.18 +2.9 2,761,711 + DZA65_RS12885 0.22 +0.6 2,761,711 + DZA65_RS12885 0.22 +1.9 2,761,711 + DZA65_RS12885 0.22 -0.1 2,761,711 + DZA65_RS12885 0.22 -0.3 2,761,711 + DZA65_RS12885 0.22 +2.4 2,761,712 - DZA65_RS12885 0.22 -1.6 2,761,718 + DZA65_RS12885 0.23 -1.1 2,761,718 + DZA65_RS12885 0.23 -2.1 2,761,719 - DZA65_RS12885 0.23 -1.9 2,761,719 - DZA65_RS12885 0.23 -1.6 2,761,719 - DZA65_RS12885 0.23 -1.9 2,761,822 + DZA65_RS12885 0.35 -1.0 2,761,823 - DZA65_RS12885 0.35 -1.6 2,761,894 + DZA65_RS12885 0.43 +2.6 2,761,895 - DZA65_RS12885 0.43 +0.2 2,761,895 - DZA65_RS12885 0.43 -1.3 2,761,895 - DZA65_RS12885 0.43 -1.3 2,761,895 - DZA65_RS12885 0.43 -1.6 2,761,950 + DZA65_RS12885 0.49 +3.6 2,761,950 + DZA65_RS12885 0.49 -1.6 2,761,951 - DZA65_RS12885 0.49 -0.9 2,761,951 - DZA65_RS12885 0.49 +0.0 2,762,026 + DZA65_RS12885 0.57 -1.3 2,762,026 + DZA65_RS12885 0.57 +1.7 2,762,027 - DZA65_RS12885 0.57 -1.6 2,762,027 - +2.0 2,762,242 + DZA65_RS12885 0.81 +2.1 2,762,242 + DZA65_RS12885 0.81 -1.9 2,762,242 + DZA65_RS12885 0.81 -0.6 2,762,270 - DZA65_RS12885 0.84 -1.6 2,762,270 - DZA65_RS12885 0.84 -1.3 2,762,333 + -0.9 2,762,333 + +0.7 2,762,333 + -2.6 2,762,333 + -2.6 2,762,333 + -2.1 2,762,333 + +1.2 2,762,333 + -0.4 2,762,333 + -0.3 2,762,333 + +0.2 2,762,334 - +0.0 2,762,334 - -1.3 2,762,334 - +0.2 2,762,334 - -1.1 2,762,334 - +0.7
Or see this region's nucleotide sequence