Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03460 and DZA65_RS03465 are separated by 313 nucleotides DZA65_RS03465 and DZA65_RS03470 overlap by 20 nucleotides DZA65_RS03470 and DZA65_RS03475 are separated by 94 nucleotides DZA65_RS03475 and DZA65_RS03480 are separated by 128 nucleotides
DZA65_RS03460: DZA65_RS03460 - tRNA-modifying protein YgfZ, at 731,705 to 732,685
_RS03460
DZA65_RS03465: DZA65_RS03465 - FAD assembly factor SdhE, at 732,999 to 733,265
_RS03465
DZA65_RS03470: DZA65_RS03470 - membrane protein, at 733,246 to 733,677
_RS03470
DZA65_RS03475: DZA65_RS03475 - flavodoxin FldB, at 733,772 to 734,290
_RS03475
DZA65_RS03480: DZA65_RS03480 - MHS family MFS transporter, at 734,419 to 735,744
_RS03480
Position (kb) 733
734 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 732.686 kb on - strand at 732.689 kb on - strand at 732.689 kb on - strand at 732.689 kb on - strand at 732.689 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.706 kb on - strand at 732.729 kb on + strand at 732.729 kb on + strand at 732.730 kb on - strand at 732.730 kb on - strand at 732.759 kb on + strand at 732.760 kb on - strand at 732.832 kb on + strand at 732.843 kb on + strand at 732.843 kb on + strand at 732.843 kb on + strand at 732.844 kb on - strand at 732.844 kb on - strand at 732.844 kb on - strand at 732.844 kb on - strand at 732.844 kb on - strand at 732.863 kb on + strand at 732.863 kb on + strand at 732.863 kb on + strand at 732.864 kb on - strand at 732.864 kb on - strand at 732.866 kb on + strand at 732.866 kb on + strand at 732.866 kb on + strand at 732.866 kb on + strand at 732.867 kb on - strand at 732.867 kb on - strand at 732.879 kb on - strand at 732.888 kb on + strand at 732.889 kb on - strand at 732.902 kb on - strand at 732.904 kb on + strand at 732.904 kb on + strand at 732.905 kb on - strand at 732.907 kb on + strand at 732.908 kb on - strand at 732.925 kb on + strand at 732.925 kb on + strand at 732.925 kb on + strand at 732.930 kb on + strand at 732.931 kb on - strand at 732.936 kb on + strand at 732.936 kb on + strand at 732.936 kb on + strand at 732.936 kb on + strand at 732.936 kb on + strand at 732.936 kb on + strand at 732.937 kb on - strand at 732.975 kb on + strand at 732.975 kb on + strand at 732.976 kb on - strand at 733.022 kb on + strand at 733.022 kb on + strand at 733.093 kb on - strand, within DZA65_RS03465 at 733.093 kb on - strand, within DZA65_RS03465 at 733.238 kb on + strand, within DZA65_RS03465 at 733.238 kb on + strand, within DZA65_RS03465 at 733.238 kb on + strand, within DZA65_RS03465 at 733.239 kb on - strand at 733.268 kb on + strand at 733.268 kb on + strand at 733.275 kb on - strand at 733.496 kb on + strand, within DZA65_RS03470 at 733.496 kb on + strand, within DZA65_RS03470 at 733.496 kb on + strand, within DZA65_RS03470 at 733.496 kb on + strand, within DZA65_RS03470 at 733.496 kb on + strand, within DZA65_RS03470 at 733.497 kb on - strand, within DZA65_RS03470 at 733.497 kb on - strand, within DZA65_RS03470 at 733.497 kb on - strand, within DZA65_RS03470 at 733.497 kb on - strand, within DZA65_RS03470 at 733.497 kb on - strand, within DZA65_RS03470 at 733.593 kb on + strand, within DZA65_RS03470 at 733.593 kb on + strand, within DZA65_RS03470 at 733.594 kb on - strand, within DZA65_RS03470 at 733.675 kb on + strand at 733.675 kb on + strand at 733.778 kb on + strand at 733.778 kb on + strand at 733.778 kb on + strand at 733.779 kb on - strand at 733.779 kb on - strand at 733.779 kb on - strand at 733.842 kb on + strand, within DZA65_RS03475 at 733.859 kb on + strand, within DZA65_RS03475 at 733.859 kb on + strand, within DZA65_RS03475 at 733.859 kb on + strand, within DZA65_RS03475 at 733.860 kb on - strand, within DZA65_RS03475 at 733.878 kb on + strand, within DZA65_RS03475 at 733.878 kb on + strand, within DZA65_RS03475 at 733.878 kb on + strand, within DZA65_RS03475 at 733.878 kb on + strand, within DZA65_RS03475 at 733.878 kb on + strand, within DZA65_RS03475 at 733.878 kb on + strand, within DZA65_RS03475 at 733.879 kb on - strand, within DZA65_RS03475 at 733.879 kb on - strand, within DZA65_RS03475 at 733.879 kb on - strand, within DZA65_RS03475 at 733.882 kb on + strand at 733.882 kb on + strand, within DZA65_RS03475 at 733.883 kb on - strand, within DZA65_RS03475 at 733.883 kb on - strand, within DZA65_RS03475 at 733.920 kb on + strand, within DZA65_RS03475 at 733.945 kb on + strand, within DZA65_RS03475 at 734.038 kb on - strand, within DZA65_RS03475 at 734.155 kb on - strand, within DZA65_RS03475 at 734.202 kb on - strand, within DZA65_RS03475 at 734.253 kb on + strand at 734.253 kb on + strand at 734.254 kb on - strand at 734.254 kb on - strand at 734.254 kb on - strand at 734.282 kb on + strand at 734.283 kb on - strand at 734.283 kb on - strand at 734.290 kb on + strand at 734.290 kb on + strand at 734.290 kb on + strand at 734.291 kb on - strand at 734.291 kb on - strand at 734.291 kb on - strand at 734.291 kb on - strand at 734.291 kb on - strand at 734.316 kb on + strand at 734.316 kb on + strand at 734.317 kb on - strand at 734.339 kb on + strand at 734.352 kb on + strand at 734.353 kb on - strand at 734.365 kb on + strand at 734.365 kb on + strand at 734.411 kb on + strand at 734.420 kb on + strand at 734.421 kb on - strand at 734.495 kb on + strand at 734.507 kb on + strand at 734.507 kb on + strand at 734.507 kb on + strand at 734.507 kb on + strand at 734.507 kb on + strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.508 kb on - strand at 734.546 kb on + strand at 734.547 kb on - strand at 734.547 kb on - strand at 734.578 kb on + strand, within DZA65_RS03480 at 734.578 kb on + strand, within DZA65_RS03480 at 734.578 kb on + strand, within DZA65_RS03480 at 734.578 kb on + strand, within DZA65_RS03480 at 734.578 kb on + strand, within DZA65_RS03480 at 734.578 kb on + strand, within DZA65_RS03480 at 734.578 kb on + strand, within DZA65_RS03480 at 734.579 kb on - strand, within DZA65_RS03480 at 734.579 kb on - strand, within DZA65_RS03480 at 734.579 kb on - strand, within DZA65_RS03480 at 734.583 kb on + strand, within DZA65_RS03480 at 734.584 kb on - strand, within DZA65_RS03480 at 734.585 kb on + strand, within DZA65_RS03480 at 734.585 kb on + strand, within DZA65_RS03480 at 734.585 kb on + strand, within DZA65_RS03480 at 734.585 kb on + strand, within DZA65_RS03480 at 734.586 kb on - strand, within DZA65_RS03480 at 734.586 kb on - strand, within DZA65_RS03480 at 734.586 kb on - strand, within DZA65_RS03480 at 734.604 kb on + strand, within DZA65_RS03480 at 734.604 kb on + strand, within DZA65_RS03480 at 734.606 kb on + strand, within DZA65_RS03480 at 734.606 kb on + strand, within DZA65_RS03480 at 734.606 kb on + strand, within DZA65_RS03480
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 732,686 - +0.4 732,689 - -2.6 732,689 - +0.2 732,689 - +0.3 732,689 - -0.3 732,706 - -1.3 732,706 - -1.8 732,706 - -0.2 732,706 - -1.3 732,706 - +0.0 732,706 - -2.0 732,706 - +2.8 732,706 - -1.9 732,706 - -2.7 732,706 - +2.7 732,729 + +1.3 732,729 + -1.8 732,730 - -1.8 732,730 - +1.0 732,759 + +0.0 732,760 - +1.0 732,832 + +1.0 732,843 + +2.0 732,843 + +1.2 732,843 + -2.9 732,844 - +0.3 732,844 - -1.4 732,844 - -2.0 732,844 - +0.5 732,844 - +1.1 732,863 + +1.5 732,863 + +0.9 732,863 + -0.4 732,864 - +1.0 732,864 - -1.6 732,866 + -3.5 732,866 + +0.4 732,866 + +1.7 732,866 + -1.2 732,867 - +0.4 732,867 - -1.7 732,879 - -3.3 732,888 + +0.7 732,889 - +0.7 732,902 - -1.2 732,904 + +0.5 732,904 + +1.0 732,905 - +0.8 732,907 + -0.5 732,908 - +1.2 732,925 + +3.2 732,925 + -1.9 732,925 + -1.7 732,930 + -1.0 732,931 - +2.3 732,936 + -1.0 732,936 + -0.7 732,936 + +0.0 732,936 + +0.3 732,936 + -1.0 732,936 + +1.0 732,937 - -2.5 732,975 + +1.7 732,975 + -0.3 732,976 - -1.0 733,022 + -3.1 733,022 + -0.6 733,093 - DZA65_RS03465 0.35 -2.2 733,093 - DZA65_RS03465 0.35 -0.6 733,238 + DZA65_RS03465 0.90 -1.0 733,238 + DZA65_RS03465 0.90 -1.3 733,238 + DZA65_RS03465 0.90 -1.4 733,239 - +0.0 733,268 + -1.3 733,268 + +0.7 733,275 - +0.1 733,496 + DZA65_RS03470 0.58 +0.3 733,496 + DZA65_RS03470 0.58 -1.0 733,496 + DZA65_RS03470 0.58 -2.0 733,496 + DZA65_RS03470 0.58 -1.6 733,496 + DZA65_RS03470 0.58 +0.0 733,497 - DZA65_RS03470 0.58 -1.8 733,497 - DZA65_RS03470 0.58 -1.3 733,497 - DZA65_RS03470 0.58 -2.5 733,497 - DZA65_RS03470 0.58 +0.5 733,497 - DZA65_RS03470 0.58 -1.0 733,593 + DZA65_RS03470 0.80 -0.6 733,593 + DZA65_RS03470 0.80 +0.4 733,594 - DZA65_RS03470 0.81 -2.6 733,675 + +3.0 733,675 + -0.7 733,778 + +0.5 733,778 + +1.9 733,778 + +0.0 733,779 - +0.8 733,779 - +1.0 733,779 - -2.2 733,842 + DZA65_RS03475 0.13 -1.0 733,859 + DZA65_RS03475 0.17 +1.3 733,859 + DZA65_RS03475 0.17 -1.0 733,859 + DZA65_RS03475 0.17 +1.0 733,860 - DZA65_RS03475 0.17 +2.5 733,878 + DZA65_RS03475 0.20 -2.7 733,878 + DZA65_RS03475 0.20 +1.2 733,878 + DZA65_RS03475 0.20 -1.3 733,878 + DZA65_RS03475 0.20 +0.3 733,878 + DZA65_RS03475 0.20 +0.3 733,878 + DZA65_RS03475 0.20 +2.9 733,879 - DZA65_RS03475 0.21 +1.2 733,879 - DZA65_RS03475 0.21 -2.2 733,879 - DZA65_RS03475 0.21 -0.6 733,882 + -1.8 733,882 + DZA65_RS03475 0.21 +1.5 733,883 - DZA65_RS03475 0.21 +1.6 733,883 - DZA65_RS03475 0.21 +3.3 733,920 + DZA65_RS03475 0.29 -1.7 733,945 + DZA65_RS03475 0.33 -0.8 734,038 - DZA65_RS03475 0.51 -0.9 734,155 - DZA65_RS03475 0.74 +1.3 734,202 - DZA65_RS03475 0.83 -3.8 734,253 + +0.0 734,253 + +0.7 734,254 - +2.9 734,254 - +0.0 734,254 - +1.7 734,282 + -1.0 734,283 - +0.8 734,283 - -2.3 734,290 + -0.2 734,290 + +0.4 734,290 + -2.8 734,291 - -2.7 734,291 - -0.6 734,291 - +0.0 734,291 - -3.2 734,291 - +0.0 734,316 + -3.7 734,316 + -1.0 734,317 - -1.0 734,339 + +2.5 734,352 + -2.3 734,353 - +0.2 734,365 + -1.0 734,365 + -0.2 734,411 + +1.0 734,420 + -0.6 734,421 - -2.2 734,495 + +1.5 734,507 + -1.3 734,507 + -2.2 734,507 + +1.0 734,507 + -1.6 734,507 + +1.0 734,508 - -2.8 734,508 - -0.6 734,508 - -0.3 734,508 - -1.0 734,508 - -2.6 734,508 - +2.9 734,508 - +0.3 734,508 - +2.5 734,508 - -1.3 734,508 - +0.0 734,508 - +0.5 734,508 - +1.0 734,546 + +1.5 734,547 - -1.6 734,547 - +0.1 734,578 + DZA65_RS03480 0.12 -1.8 734,578 + DZA65_RS03480 0.12 +1.0 734,578 + DZA65_RS03480 0.12 +0.0 734,578 + DZA65_RS03480 0.12 -1.6 734,578 + DZA65_RS03480 0.12 -0.6 734,578 + DZA65_RS03480 0.12 +0.3 734,578 + DZA65_RS03480 0.12 +1.0 734,579 - DZA65_RS03480 0.12 +1.0 734,579 - DZA65_RS03480 0.12 -0.3 734,579 - DZA65_RS03480 0.12 -0.6 734,583 + DZA65_RS03480 0.12 +2.2 734,584 - DZA65_RS03480 0.12 +0.4 734,585 + DZA65_RS03480 0.13 -1.6 734,585 + DZA65_RS03480 0.13 +1.4 734,585 + DZA65_RS03480 0.13 -2.2 734,585 + DZA65_RS03480 0.13 -0.6 734,586 - DZA65_RS03480 0.13 -2.0 734,586 - DZA65_RS03480 0.13 +1.1 734,586 - DZA65_RS03480 0.13 +1.5 734,604 + DZA65_RS03480 0.14 +0.0 734,604 + DZA65_RS03480 0.14 -0.6 734,606 + DZA65_RS03480 0.14 -1.0 734,606 + DZA65_RS03480 0.14 +0.1 734,606 + DZA65_RS03480 0.14 +0.4
Or see this region's nucleotide sequence