Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01510 and DZA65_RS01515 are separated by 49 nucleotides DZA65_RS01515 and DZA65_RS01520 are separated by 15 nucleotides DZA65_RS01520 and DZA65_RS01525 overlap by 23 nucleotides
DZA65_RS01510: DZA65_RS01510 - RNA polymerase factor sigma-54, at 331,473 to 332,906
_RS01510
DZA65_RS01515: DZA65_RS01515 - LPS export ABC transporter ATP-binding protein, at 332,956 to 333,681
_RS01515
DZA65_RS01520: DZA65_RS01520 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 333,697 to 334,275
_RS01520
DZA65_RS01525: DZA65_RS01525 - LPS export ABC transporter periplasmic protein LptC, at 334,253 to 334,822
_RS01525
Position (kb) 332
333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 332.000 kb on - strand, within DZA65_RS01510 at 332.070 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.169 kb on + strand, within DZA65_RS01510 at 332.169 kb on + strand, within DZA65_RS01510 at 332.170 kb on - strand, within DZA65_RS01510 at 332.170 kb on - strand, within DZA65_RS01510 at 332.170 kb on - strand at 332.283 kb on - strand, within DZA65_RS01510 at 332.283 kb on - strand, within DZA65_RS01510 at 332.440 kb on - strand, within DZA65_RS01510 at 332.540 kb on - strand, within DZA65_RS01510 at 332.572 kb on + strand, within DZA65_RS01510 at 332.573 kb on - strand, within DZA65_RS01510 at 332.675 kb on + strand, within DZA65_RS01510 at 332.850 kb on + strand at 332.865 kb on - strand at 332.907 kb on - strand at 332.916 kb on + strand at 332.916 kb on + strand at 332.917 kb on - strand at 332.917 kb on - strand at 332.942 kb on - strand at 332.942 kb on - strand at 332.942 kb on - strand at 332.942 kb on - strand at 332.955 kb on - strand at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.407 kb on - strand, within DZA65_RS01525 at 334.413 kb on - strand, within DZA65_RS01525 at 334.413 kb on - strand, within DZA65_RS01525 at 334.413 kb on - strand, within DZA65_RS01525
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 332,000 - DZA65_RS01510 0.37 -0.4 332,070 - DZA65_RS01510 0.42 +0.2 332,096 - DZA65_RS01510 0.43 +0.9 332,096 - DZA65_RS01510 0.43 -1.4 332,096 - DZA65_RS01510 0.43 -1.8 332,096 - DZA65_RS01510 0.43 -3.4 332,169 + DZA65_RS01510 0.49 +5.1 332,169 + DZA65_RS01510 0.49 +1.2 332,170 - DZA65_RS01510 0.49 +0.2 332,170 - DZA65_RS01510 0.49 +1.4 332,170 - -0.8 332,283 - DZA65_RS01510 0.56 -1.1 332,283 - DZA65_RS01510 0.56 +0.2 332,440 - DZA65_RS01510 0.67 -1.1 332,540 - DZA65_RS01510 0.74 -0.8 332,572 + DZA65_RS01510 0.77 -2.1 332,573 - DZA65_RS01510 0.77 -1.4 332,675 + DZA65_RS01510 0.84 -0.5 332,850 + +1.8 332,865 - +1.2 332,907 - -1.6 332,916 + -1.4 332,916 + -0.9 332,917 - -1.1 332,917 - -1.6 332,942 - -1.9 332,942 - +1.2 332,942 - -1.1 332,942 - -1.4 332,955 - +2.0 334,366 - DZA65_RS01525 0.20 -2.4 334,366 - DZA65_RS01525 0.20 -0.4 334,366 - DZA65_RS01525 0.20 -1.6 334,366 - DZA65_RS01525 0.20 -1.4 334,366 - DZA65_RS01525 0.20 +0.2 334,366 - DZA65_RS01525 0.20 -1.6 334,407 - DZA65_RS01525 0.27 +1.2 334,413 - DZA65_RS01525 0.28 -3.0 334,413 - DZA65_RS01525 0.28 -0.4 334,413 - DZA65_RS01525 0.28 -2.7
Or see this region's nucleotide sequence