Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS12415 and DZA65_RS12420 are separated by 230 nucleotides DZA65_RS12420 and DZA65_RS12425 are separated by 407 nucleotides DZA65_RS12425 and DZA65_RS12430 are separated by 27 nucleotides
DZA65_RS12415: DZA65_RS12415 - ABC transporter ATP-binding protein, at 2,654,108 to 2,655,202
_RS12415
DZA65_RS12420: DZA65_RS12420 - tautomerase PptA, at 2,655,433 to 2,655,663
_RS12420
DZA65_RS12425: DZA65_RS12425 - DUF1852 domain-containing protein, at 2,656,071 to 2,657,048
_RS12425
DZA65_RS12430: DZA65_RS12430 - methionine synthase, at 2,657,076 to 2,658,107
_RS12430
Position (kb)
2656
2657
2658 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2655.089 kb on - strand, within DZA65_RS12415 at 2655.089 kb on - strand, within DZA65_RS12415 at 2655.200 kb on + strand at 2655.204 kb on + strand at 2655.205 kb on - strand at 2655.257 kb on - strand at 2655.274 kb on + strand at 2655.293 kb on - strand at 2655.293 kb on - strand at 2655.311 kb on - strand at 2655.323 kb on + strand at 2655.324 kb on - strand at 2655.349 kb on + strand at 2655.350 kb on - strand at 2655.350 kb on - strand at 2655.350 kb on - strand at 2655.350 kb on - strand at 2655.350 kb on - strand at 2655.350 kb on - strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.351 kb on + strand at 2655.352 kb on - strand at 2655.352 kb on - strand at 2655.352 kb on - strand at 2655.352 kb on - strand at 2655.352 kb on - strand at 2655.352 kb on - strand at 2655.352 kb on - strand at 2655.352 kb on - strand at 2655.400 kb on - strand at 2655.403 kb on - strand at 2655.428 kb on + strand at 2655.429 kb on - strand at 2655.454 kb on + strand at 2655.454 kb on + strand at 2655.495 kb on - strand, within DZA65_RS12420 at 2655.495 kb on - strand, within DZA65_RS12420 at 2655.527 kb on - strand, within DZA65_RS12420 at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.654 kb on + strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.655 kb on - strand at 2655.661 kb on + strand at 2655.661 kb on + strand at 2655.661 kb on + strand at 2655.661 kb on + strand at 2655.661 kb on + strand at 2655.662 kb on - strand at 2655.662 kb on - strand at 2655.708 kb on + strand at 2655.779 kb on + strand at 2655.780 kb on - strand at 2655.819 kb on + strand at 2655.875 kb on + strand at 2655.876 kb on - strand at 2655.876 kb on - strand at 2655.881 kb on + strand at 2655.882 kb on - strand at 2655.882 kb on - strand at 2655.882 kb on - strand at 2655.897 kb on + strand at 2655.898 kb on - strand at 2655.898 kb on - strand at 2655.898 kb on - strand at 2655.898 kb on - strand at 2655.898 kb on - strand at 2655.921 kb on - strand at 2655.921 kb on - strand at 2655.969 kb on - strand at 2656.098 kb on - strand at 2656.122 kb on + strand at 2656.169 kb on - strand, within DZA65_RS12425 at 2656.246 kb on - strand, within DZA65_RS12425 at 2656.423 kb on - strand, within DZA65_RS12425 at 2656.434 kb on + strand, within DZA65_RS12425 at 2656.447 kb on + strand, within DZA65_RS12425 at 2656.448 kb on - strand, within DZA65_RS12425 at 2656.523 kb on - strand, within DZA65_RS12425 at 2656.527 kb on - strand, within DZA65_RS12425 at 2656.582 kb on + strand, within DZA65_RS12425 at 2656.582 kb on + strand, within DZA65_RS12425 at 2656.583 kb on - strand, within DZA65_RS12425 at 2656.611 kb on + strand, within DZA65_RS12425 at 2656.612 kb on - strand, within DZA65_RS12425 at 2656.612 kb on - strand, within DZA65_RS12425 at 2656.654 kb on - strand, within DZA65_RS12425 at 2656.664 kb on + strand, within DZA65_RS12425 at 2656.664 kb on + strand, within DZA65_RS12425 at 2656.665 kb on - strand, within DZA65_RS12425 at 2656.706 kb on - strand, within DZA65_RS12425 at 2656.717 kb on - strand, within DZA65_RS12425 at 2656.721 kb on + strand, within DZA65_RS12425 at 2656.772 kb on - strand, within DZA65_RS12425 at 2656.782 kb on + strand, within DZA65_RS12425 at 2656.881 kb on + strand, within DZA65_RS12425 at 2656.881 kb on + strand, within DZA65_RS12425 at 2656.884 kb on + strand, within DZA65_RS12425 at 2656.885 kb on - strand, within DZA65_RS12425 at 2657.046 kb on + strand at 2657.046 kb on + strand at 2657.047 kb on - strand at 2657.060 kb on + strand at 2657.061 kb on - strand at 2657.068 kb on + strand at 2657.068 kb on + strand at 2657.072 kb on + strand at 2657.072 kb on + strand at 2657.073 kb on - strand at 2657.073 kb on - strand at 2657.220 kb on + strand, within DZA65_RS12430 at 2657.220 kb on + strand, within DZA65_RS12430 at 2657.240 kb on + strand, within DZA65_RS12430 at 2657.240 kb on + strand, within DZA65_RS12430 at 2657.240 kb on + strand, within DZA65_RS12430 at 2657.241 kb on - strand, within DZA65_RS12430 at 2657.244 kb on - strand, within DZA65_RS12430 at 2657.244 kb on - strand, within DZA65_RS12430 at 2657.246 kb on + strand, within DZA65_RS12430 at 2657.247 kb on - strand, within DZA65_RS12430 at 2657.355 kb on + strand, within DZA65_RS12430 at 2657.468 kb on - strand, within DZA65_RS12430 at 2657.493 kb on - strand, within DZA65_RS12430 at 2657.499 kb on + strand, within DZA65_RS12430 at 2657.499 kb on + strand, within DZA65_RS12430 at 2657.499 kb on + strand, within DZA65_RS12430 at 2657.511 kb on + strand, within DZA65_RS12430 at 2657.512 kb on - strand, within DZA65_RS12430 at 2657.514 kb on - strand, within DZA65_RS12430 at 2657.514 kb on - strand, within DZA65_RS12430 at 2657.514 kb on - strand, within DZA65_RS12430 at 2657.575 kb on + strand, within DZA65_RS12430 at 2657.594 kb on + strand, within DZA65_RS12430 at 2657.594 kb on + strand, within DZA65_RS12430 at 2657.595 kb on - strand, within DZA65_RS12430 at 2657.595 kb on - strand, within DZA65_RS12430 at 2657.621 kb on + strand, within DZA65_RS12430 at 2657.621 kb on + strand, within DZA65_RS12430 at 2657.621 kb on + strand, within DZA65_RS12430 at 2657.622 kb on - strand, within DZA65_RS12430 at 2657.666 kb on - strand, within DZA65_RS12430 at 2657.666 kb on - strand at 2657.704 kb on + strand, within DZA65_RS12430 at 2657.704 kb on + strand at 2657.704 kb on + strand, within DZA65_RS12430 at 2657.704 kb on + strand, within DZA65_RS12430 at 2657.704 kb on + strand, within DZA65_RS12430 at 2657.704 kb on + strand, within DZA65_RS12430 at 2657.705 kb on - strand, within DZA65_RS12430 at 2657.705 kb on - strand, within DZA65_RS12430 at 2657.705 kb on - strand, within DZA65_RS12430 at 2657.705 kb on - strand, within DZA65_RS12430 at 2657.705 kb on - strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.708 kb on + strand, within DZA65_RS12430 at 2657.709 kb on - strand, within DZA65_RS12430 at 2657.709 kb on - strand, within DZA65_RS12430 at 2657.709 kb on - strand, within DZA65_RS12430 at 2657.715 kb on + strand, within DZA65_RS12430 at 2657.778 kb on + strand, within DZA65_RS12430 at 2657.779 kb on - strand, within DZA65_RS12430 at 2657.779 kb on - strand, within DZA65_RS12430 at 2657.779 kb on - strand, within DZA65_RS12430 at 2657.840 kb on + strand, within DZA65_RS12430 at 2657.921 kb on + strand, within DZA65_RS12430 at 2657.922 kb on - strand, within DZA65_RS12430 at 2657.952 kb on - strand, within DZA65_RS12430 at 2657.952 kb on - strand, within DZA65_RS12430 at 2657.994 kb on + strand, within DZA65_RS12430 at 2657.994 kb on + strand, within DZA65_RS12430 at 2658.026 kb on - strand at 2658.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 2,655,089 - DZA65_RS12415 0.90 -0.5 2,655,089 - DZA65_RS12415 0.90 -0.9 2,655,200 + -0.3 2,655,204 + +0.1 2,655,205 - -1.8 2,655,257 - +0.1 2,655,274 + -2.5 2,655,293 - -0.8 2,655,293 - -0.5 2,655,311 - -0.3 2,655,323 + -0.1 2,655,324 - +1.1 2,655,349 + +2.8 2,655,350 - +1.5 2,655,350 - +0.7 2,655,350 - -0.5 2,655,350 - +1.8 2,655,350 - -2.3 2,655,350 - -0.4 2,655,351 + -1.9 2,655,351 + -1.3 2,655,351 + -1.3 2,655,351 + +1.1 2,655,351 + +0.9 2,655,351 + -0.9 2,655,351 + +1.1 2,655,351 + +0.7 2,655,351 + -0.3 2,655,351 + +0.2 2,655,351 + -0.8 2,655,352 - +0.1 2,655,352 - +3.4 2,655,352 - +1.4 2,655,352 - -0.6 2,655,352 - +0.2 2,655,352 - +1.6 2,655,352 - -1.4 2,655,352 - -1.8 2,655,400 - +0.6 2,655,403 - -0.9 2,655,428 + -1.5 2,655,429 - +0.2 2,655,454 + -2.8 2,655,454 + -0.5 2,655,495 - DZA65_RS12420 0.27 -1.3 2,655,495 - DZA65_RS12420 0.27 -1.9 2,655,527 - DZA65_RS12420 0.41 -2.4 2,655,654 + -1.1 2,655,654 + +1.5 2,655,654 + +0.1 2,655,654 + -1.8 2,655,654 + -0.9 2,655,654 + +1.6 2,655,654 + +0.1 2,655,654 + -0.5 2,655,654 + +0.1 2,655,654 + +1.1 2,655,654 + -0.4 2,655,654 + -2.1 2,655,655 - -0.5 2,655,655 - -0.4 2,655,655 - +0.1 2,655,655 - -2.1 2,655,655 - -1.3 2,655,655 - +2.6 2,655,655 - -0.9 2,655,655 - -0.8 2,655,655 - -0.0 2,655,661 + +1.4 2,655,661 + +3.3 2,655,661 + +2.1 2,655,661 + -2.5 2,655,661 + -0.9 2,655,662 - -0.9 2,655,662 - -0.5 2,655,708 + +0.1 2,655,779 + +0.5 2,655,780 - -0.9 2,655,819 + +3.6 2,655,875 + +1.6 2,655,876 - -0.1 2,655,876 - +4.9 2,655,881 + +0.3 2,655,882 - +0.1 2,655,882 - +2.3 2,655,882 - -0.9 2,655,897 + -0.9 2,655,898 - -1.3 2,655,898 - -0.9 2,655,898 - -0.6 2,655,898 - +1.2 2,655,898 - -0.4 2,655,921 - -2.4 2,655,921 - -1.3 2,655,969 - -0.1 2,656,098 - +0.5 2,656,122 + -0.4 2,656,169 - DZA65_RS12425 0.10 +3.8 2,656,246 - DZA65_RS12425 0.18 +1.1 2,656,423 - DZA65_RS12425 0.36 -3.1 2,656,434 + DZA65_RS12425 0.37 +0.5 2,656,447 + DZA65_RS12425 0.38 -1.4 2,656,448 - DZA65_RS12425 0.39 +0.8 2,656,523 - DZA65_RS12425 0.46 -1.5 2,656,527 - DZA65_RS12425 0.47 -0.5 2,656,582 + DZA65_RS12425 0.52 -0.3 2,656,582 + DZA65_RS12425 0.52 -2.6 2,656,583 - DZA65_RS12425 0.52 -0.1 2,656,611 + DZA65_RS12425 0.55 -1.8 2,656,612 - DZA65_RS12425 0.55 +2.1 2,656,612 - DZA65_RS12425 0.55 -0.5 2,656,654 - DZA65_RS12425 0.60 -2.1 2,656,664 + DZA65_RS12425 0.61 -1.8 2,656,664 + DZA65_RS12425 0.61 +0.1 2,656,665 - DZA65_RS12425 0.61 -1.3 2,656,706 - DZA65_RS12425 0.65 -1.8 2,656,717 - DZA65_RS12425 0.66 +1.4 2,656,721 + DZA65_RS12425 0.66 +1.5 2,656,772 - DZA65_RS12425 0.72 +0.8 2,656,782 + DZA65_RS12425 0.73 -0.3 2,656,881 + DZA65_RS12425 0.83 +2.9 2,656,881 + DZA65_RS12425 0.83 -0.3 2,656,884 + DZA65_RS12425 0.83 -0.5 2,656,885 - DZA65_RS12425 0.83 -1.5 2,657,046 + -1.3 2,657,046 + -1.4 2,657,047 - +0.2 2,657,060 + -1.8 2,657,061 - +2.3 2,657,068 + -1.9 2,657,068 + +1.8 2,657,072 + -1.6 2,657,072 + -0.5 2,657,073 - -1.5 2,657,073 - +0.1 2,657,220 + DZA65_RS12430 0.14 -0.2 2,657,220 + DZA65_RS12430 0.14 -2.6 2,657,240 + DZA65_RS12430 0.16 +0.7 2,657,240 + DZA65_RS12430 0.16 +1.3 2,657,240 + DZA65_RS12430 0.16 +1.1 2,657,241 - DZA65_RS12430 0.16 +1.7 2,657,244 - DZA65_RS12430 0.16 +0.8 2,657,244 - DZA65_RS12430 0.16 -1.3 2,657,246 + DZA65_RS12430 0.16 -0.4 2,657,247 - DZA65_RS12430 0.17 -1.1 2,657,355 + DZA65_RS12430 0.27 +1.2 2,657,468 - DZA65_RS12430 0.38 -0.8 2,657,493 - DZA65_RS12430 0.40 +0.5 2,657,499 + DZA65_RS12430 0.41 +0.6 2,657,499 + DZA65_RS12430 0.41 -1.3 2,657,499 + DZA65_RS12430 0.41 -0.5 2,657,511 + DZA65_RS12430 0.42 -0.2 2,657,512 - DZA65_RS12430 0.42 -2.3 2,657,514 - DZA65_RS12430 0.42 +1.1 2,657,514 - DZA65_RS12430 0.42 +1.1 2,657,514 - DZA65_RS12430 0.42 -1.3 2,657,575 + DZA65_RS12430 0.48 +1.3 2,657,594 + DZA65_RS12430 0.50 +0.5 2,657,594 + DZA65_RS12430 0.50 +1.0 2,657,595 - DZA65_RS12430 0.50 -0.5 2,657,595 - DZA65_RS12430 0.50 +2.5 2,657,621 + DZA65_RS12430 0.53 +2.2 2,657,621 + DZA65_RS12430 0.53 -0.9 2,657,621 + DZA65_RS12430 0.53 +1.1 2,657,622 - DZA65_RS12430 0.53 +1.1 2,657,666 - DZA65_RS12430 0.57 -0.5 2,657,666 - +1.1 2,657,704 + DZA65_RS12430 0.61 +1.8 2,657,704 + -0.5 2,657,704 + DZA65_RS12430 0.61 +1.1 2,657,704 + DZA65_RS12430 0.61 -1.5 2,657,704 + DZA65_RS12430 0.61 -0.9 2,657,704 + DZA65_RS12430 0.61 +1.2 2,657,705 - DZA65_RS12430 0.61 +0.1 2,657,705 - DZA65_RS12430 0.61 -1.3 2,657,705 - DZA65_RS12430 0.61 -0.1 2,657,705 - DZA65_RS12430 0.61 -2.1 2,657,705 - DZA65_RS12430 0.61 -0.9 2,657,708 + DZA65_RS12430 0.61 -1.9 2,657,708 + DZA65_RS12430 0.61 +0.7 2,657,708 + DZA65_RS12430 0.61 -1.1 2,657,708 + DZA65_RS12430 0.61 +2.1 2,657,708 + DZA65_RS12430 0.61 +0.1 2,657,708 + DZA65_RS12430 0.61 +0.1 2,657,708 + DZA65_RS12430 0.61 -2.8 2,657,708 + DZA65_RS12430 0.61 +0.1 2,657,708 + DZA65_RS12430 0.61 -1.5 2,657,709 - DZA65_RS12430 0.61 +0.1 2,657,709 - DZA65_RS12430 0.61 +0.5 2,657,709 - DZA65_RS12430 0.61 -1.8 2,657,715 + DZA65_RS12430 0.62 -1.3 2,657,778 + DZA65_RS12430 0.68 -1.3 2,657,779 - DZA65_RS12430 0.68 +0.1 2,657,779 - DZA65_RS12430 0.68 +2.3 2,657,779 - DZA65_RS12430 0.68 +0.7 2,657,840 + DZA65_RS12430 0.74 -1.8 2,657,921 + DZA65_RS12430 0.82 -1.5 2,657,922 - DZA65_RS12430 0.82 -1.3 2,657,952 - DZA65_RS12430 0.85 -1.3 2,657,952 - DZA65_RS12430 0.85 -2.6 2,657,994 + DZA65_RS12430 0.89 +3.1 2,657,994 + DZA65_RS12430 0.89 +0.3 2,658,026 - -0.7 2,658,026 - -1.5
Or see this region's nucleotide sequence