Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10785 and DZA65_RS10790 are separated by 5 nucleotides DZA65_RS10790 and DZA65_RS10795 are separated by 45 nucleotides DZA65_RS10795 and DZA65_RS10800 are separated by 358 nucleotides
DZA65_RS10785: DZA65_RS10785 - DNA topoisomerase III, at 2,282,246 to 2,284,171
_RS10785
DZA65_RS10790: DZA65_RS10790 - DUF1496 domain-containing protein, at 2,284,177 to 2,284,572
_RS10790
DZA65_RS10795: DZA65_RS10795 - exodeoxyribonuclease III, at 2,284,618 to 2,285,424
_RS10795
DZA65_RS10800: DZA65_RS10800 - trimeric intracellular cation channel family protein, at 2,285,783 to 2,286,403
_RS10800
Position (kb)
2284
2285
2286 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2283.765 kb on + strand, within DZA65_RS10785 at 2283.766 kb on - strand at 2283.884 kb on + strand, within DZA65_RS10785 at 2283.884 kb on + strand, within DZA65_RS10785 at 2283.885 kb on - strand at 2283.886 kb on + strand, within DZA65_RS10785 at 2283.886 kb on + strand at 2283.886 kb on + strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2283.887 kb on - strand, within DZA65_RS10785 at 2284.051 kb on + strand at 2284.052 kb on - strand at 2284.075 kb on + strand at 2284.076 kb on - strand at 2284.078 kb on + strand at 2284.079 kb on - strand at 2284.220 kb on + strand, within DZA65_RS10790 at 2284.220 kb on + strand, within DZA65_RS10790 at 2284.260 kb on + strand, within DZA65_RS10790 at 2284.261 kb on - strand, within DZA65_RS10790 at 2284.281 kb on - strand, within DZA65_RS10790 at 2284.302 kb on + strand, within DZA65_RS10790 at 2284.374 kb on + strand, within DZA65_RS10790 at 2284.460 kb on - strand, within DZA65_RS10790 at 2284.460 kb on - strand, within DZA65_RS10790 at 2284.570 kb on + strand at 2284.577 kb on - strand at 2284.577 kb on - strand at 2284.577 kb on - strand at 2284.577 kb on - strand at 2284.683 kb on - strand at 2284.683 kb on - strand at 2284.732 kb on - strand, within DZA65_RS10795 at 2284.939 kb on - strand, within DZA65_RS10795 at 2284.939 kb on - strand, within DZA65_RS10795 at 2284.981 kb on - strand, within DZA65_RS10795 at 2285.021 kb on + strand, within DZA65_RS10795 at 2285.021 kb on + strand, within DZA65_RS10795 at 2285.021 kb on + strand, within DZA65_RS10795 at 2285.022 kb on - strand, within DZA65_RS10795 at 2285.022 kb on - strand, within DZA65_RS10795 at 2285.039 kb on + strand, within DZA65_RS10795 at 2285.040 kb on - strand, within DZA65_RS10795 at 2285.040 kb on - strand, within DZA65_RS10795 at 2285.040 kb on - strand, within DZA65_RS10795 at 2285.154 kb on + strand, within DZA65_RS10795 at 2285.238 kb on - strand, within DZA65_RS10795 at 2285.238 kb on - strand, within DZA65_RS10795 at 2285.259 kb on - strand, within DZA65_RS10795 at 2285.304 kb on + strand, within DZA65_RS10795 at 2285.304 kb on + strand, within DZA65_RS10795 at 2285.305 kb on - strand, within DZA65_RS10795 at 2285.305 kb on - strand, within DZA65_RS10795 at 2285.305 kb on - strand, within DZA65_RS10795 at 2285.305 kb on - strand, within DZA65_RS10795 at 2285.403 kb on + strand at 2285.462 kb on - strand at 2285.494 kb on - strand at 2285.515 kb on + strand at 2285.515 kb on + strand at 2285.515 kb on + strand at 2285.515 kb on + strand at 2285.515 kb on + strand at 2285.515 kb on + strand at 2285.515 kb on + strand at 2285.516 kb on - strand at 2285.516 kb on - strand at 2285.516 kb on - strand at 2285.516 kb on - strand at 2285.516 kb on - strand at 2285.519 kb on + strand at 2285.520 kb on - strand at 2285.558 kb on + strand at 2285.559 kb on - strand at 2285.561 kb on - strand at 2285.561 kb on - strand at 2285.561 kb on - strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.592 kb on + strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.593 kb on - strand at 2285.636 kb on - strand at 2285.653 kb on + strand at 2285.653 kb on + strand at 2285.654 kb on - strand at 2285.741 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.792 kb on + strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.793 kb on - strand at 2285.794 kb on + strand at 2285.794 kb on + strand at 2285.794 kb on + strand at 2285.794 kb on + strand at 2285.794 kb on + strand at 2285.795 kb on - strand at 2285.798 kb on + strand at 2285.798 kb on + strand at 2285.798 kb on + strand at 2285.798 kb on + strand at 2285.798 kb on + strand at 2285.798 kb on + strand at 2285.798 kb on + strand at 2285.799 kb on - strand at 2285.799 kb on - strand at 2285.799 kb on - strand at 2285.799 kb on - strand at 2285.799 kb on - strand at 2285.799 kb on - strand at 2285.799 kb on - strand at 2285.799 kb on - strand at 2285.809 kb on + strand at 2285.810 kb on - strand at 2285.810 kb on - strand at 2285.891 kb on - strand, within DZA65_RS10800 at 2285.940 kb on - strand, within DZA65_RS10800 at 2285.969 kb on + strand, within DZA65_RS10800 at 2285.969 kb on + strand, within DZA65_RS10800 at 2285.969 kb on + strand, within DZA65_RS10800 at 2285.969 kb on + strand, within DZA65_RS10800 at 2285.969 kb on + strand, within DZA65_RS10800 at 2285.969 kb on + strand, within DZA65_RS10800 at 2285.969 kb on + strand, within DZA65_RS10800 at 2285.970 kb on - strand, within DZA65_RS10800 at 2285.970 kb on - strand, within DZA65_RS10800 at 2285.970 kb on - strand, within DZA65_RS10800 at 2285.970 kb on - strand, within DZA65_RS10800 at 2285.970 kb on - strand, within DZA65_RS10800 at 2285.971 kb on + strand, within DZA65_RS10800 at 2285.971 kb on + strand, within DZA65_RS10800 at 2285.971 kb on + strand, within DZA65_RS10800 at 2285.972 kb on - strand, within DZA65_RS10800 at 2285.972 kb on - strand, within DZA65_RS10800 at 2285.972 kb on - strand, within DZA65_RS10800 at 2285.972 kb on - strand, within DZA65_RS10800 at 2285.972 kb on - strand, within DZA65_RS10800 at 2285.972 kb on - strand, within DZA65_RS10800 at 2285.975 kb on + strand, within DZA65_RS10800 at 2285.975 kb on + strand, within DZA65_RS10800 at 2285.975 kb on + strand, within DZA65_RS10800 at 2285.976 kb on - strand, within DZA65_RS10800 at 2285.976 kb on - strand, within DZA65_RS10800 at 2285.976 kb on - strand, within DZA65_RS10800 at 2285.976 kb on - strand, within DZA65_RS10800 at 2285.976 kb on - strand, within DZA65_RS10800 at 2285.976 kb on - strand, within DZA65_RS10800 at 2286.079 kb on + strand, within DZA65_RS10800 at 2286.079 kb on + strand, within DZA65_RS10800 at 2286.080 kb on - strand, within DZA65_RS10800 at 2286.080 kb on - strand, within DZA65_RS10800 at 2286.080 kb on - strand, within DZA65_RS10800 at 2286.080 kb on - strand, within DZA65_RS10800 at 2286.086 kb on - strand, within DZA65_RS10800 at 2286.148 kb on + strand, within DZA65_RS10800 at 2286.148 kb on + strand, within DZA65_RS10800 at 2286.148 kb on + strand, within DZA65_RS10800 at 2286.148 kb on + strand, within DZA65_RS10800 at 2286.158 kb on - strand, within DZA65_RS10800 at 2286.174 kb on + strand, within DZA65_RS10800 at 2286.181 kb on + strand, within DZA65_RS10800 at 2286.181 kb on + strand, within DZA65_RS10800 at 2286.224 kb on + strand, within DZA65_RS10800 at 2286.224 kb on + strand, within DZA65_RS10800 at 2286.224 kb on + strand, within DZA65_RS10800 at 2286.224 kb on + strand, within DZA65_RS10800 at 2286.224 kb on + strand, within DZA65_RS10800 at 2286.224 kb on + strand, within DZA65_RS10800 at 2286.225 kb on - strand, within DZA65_RS10800 at 2286.225 kb on - strand, within DZA65_RS10800 at 2286.225 kb on - strand at 2286.294 kb on + strand, within DZA65_RS10800 at 2286.295 kb on - strand, within DZA65_RS10800 at 2286.325 kb on - strand, within DZA65_RS10800 at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.377 kb on + strand at 2286.378 kb on - strand at 2286.378 kb on - strand at 2286.378 kb on - strand at 2286.378 kb on - strand at 2286.378 kb on - strand at 2286.378 kb on - strand at 2286.378 kb on - strand at 2286.379 kb on + strand at 2286.379 kb on + strand at 2286.379 kb on + strand at 2286.380 kb on - strand at 2286.380 kb on - strand at 2286.380 kb on - strand at 2286.380 kb on - strand at 2286.380 kb on - strand at 2286.401 kb on + strand at 2286.401 kb on + strand at 2286.402 kb on - strand at 2286.421 kb on + strand at 2286.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 2,283,765 + DZA65_RS10785 0.79 -0.5 2,283,766 - -0.9 2,283,884 + DZA65_RS10785 0.85 -0.7 2,283,884 + DZA65_RS10785 0.85 -1.2 2,283,885 - -1.9 2,283,886 + DZA65_RS10785 0.85 -1.2 2,283,886 + -1.5 2,283,886 + DZA65_RS10785 0.85 +1.7 2,283,887 - DZA65_RS10785 0.85 -0.5 2,283,887 - DZA65_RS10785 0.85 -0.3 2,283,887 - DZA65_RS10785 0.85 +0.3 2,283,887 - DZA65_RS10785 0.85 -1.0 2,283,887 - DZA65_RS10785 0.85 +0.5 2,284,051 + -2.7 2,284,052 - +1.7 2,284,075 + +0.3 2,284,076 - -1.3 2,284,078 + -0.4 2,284,079 - -1.5 2,284,220 + DZA65_RS10790 0.11 +0.1 2,284,220 + DZA65_RS10790 0.11 -0.5 2,284,260 + DZA65_RS10790 0.21 +0.1 2,284,261 - DZA65_RS10790 0.21 +1.1 2,284,281 - DZA65_RS10790 0.26 +0.1 2,284,302 + DZA65_RS10790 0.32 -0.9 2,284,374 + DZA65_RS10790 0.50 +0.5 2,284,460 - DZA65_RS10790 0.71 +2.0 2,284,460 - DZA65_RS10790 0.71 +1.1 2,284,570 + -0.9 2,284,577 - +0.3 2,284,577 - -1.2 2,284,577 - -0.7 2,284,577 - +1.3 2,284,683 - -1.7 2,284,683 - +0.7 2,284,732 - DZA65_RS10795 0.14 -0.5 2,284,939 - DZA65_RS10795 0.40 -2.3 2,284,939 - DZA65_RS10795 0.40 +1.1 2,284,981 - DZA65_RS10795 0.45 -2.0 2,285,021 + DZA65_RS10795 0.50 -1.2 2,285,021 + DZA65_RS10795 0.50 -2.0 2,285,021 + DZA65_RS10795 0.50 +3.5 2,285,022 - DZA65_RS10795 0.50 -1.8 2,285,022 - DZA65_RS10795 0.50 +1.1 2,285,039 + DZA65_RS10795 0.52 -1.7 2,285,040 - DZA65_RS10795 0.52 -0.1 2,285,040 - DZA65_RS10795 0.52 +1.3 2,285,040 - DZA65_RS10795 0.52 -1.2 2,285,154 + DZA65_RS10795 0.66 -0.1 2,285,238 - DZA65_RS10795 0.77 -0.2 2,285,238 - DZA65_RS10795 0.77 +0.1 2,285,259 - DZA65_RS10795 0.79 +1.1 2,285,304 + DZA65_RS10795 0.85 -1.6 2,285,304 + DZA65_RS10795 0.85 -1.9 2,285,305 - DZA65_RS10795 0.85 -0.2 2,285,305 - DZA65_RS10795 0.85 +1.7 2,285,305 - DZA65_RS10795 0.85 -1.9 2,285,305 - DZA65_RS10795 0.85 +0.1 2,285,403 + -0.9 2,285,462 - -0.0 2,285,494 - +1.8 2,285,515 + +0.1 2,285,515 + +0.5 2,285,515 + +1.1 2,285,515 + -0.9 2,285,515 + -1.2 2,285,515 + -0.2 2,285,515 + +0.1 2,285,516 - +1.1 2,285,516 - -0.6 2,285,516 - +0.6 2,285,516 - -2.0 2,285,516 - -1.7 2,285,519 + -1.7 2,285,520 - -0.1 2,285,558 + +1.4 2,285,559 - -0.2 2,285,561 - -1.9 2,285,561 - -0.9 2,285,561 - -0.9 2,285,592 + +0.1 2,285,592 + +1.6 2,285,592 + -1.0 2,285,592 + -0.5 2,285,592 + -0.9 2,285,592 + -0.9 2,285,592 + +0.5 2,285,592 + +2.0 2,285,592 + +0.8 2,285,592 + -2.1 2,285,592 + -0.5 2,285,592 + -1.9 2,285,592 + -0.9 2,285,593 - +0.4 2,285,593 - -1.7 2,285,593 - -2.2 2,285,593 - +0.8 2,285,593 - -0.5 2,285,593 - +0.6 2,285,593 - -0.4 2,285,593 - +0.6 2,285,593 - -2.9 2,285,636 - +0.1 2,285,653 + -0.5 2,285,653 + +0.1 2,285,654 - +0.4 2,285,741 + -0.9 2,285,792 + -1.6 2,285,792 + -1.2 2,285,792 + +1.1 2,285,792 + -0.5 2,285,792 + -1.4 2,285,792 + +0.1 2,285,792 + -2.0 2,285,792 + +1.1 2,285,792 + -1.7 2,285,792 + -1.5 2,285,792 + -1.7 2,285,792 + +0.6 2,285,792 + -1.9 2,285,792 + -1.4 2,285,792 + +1.1 2,285,792 + +0.2 2,285,793 - -1.8 2,285,793 - +0.1 2,285,793 - +0.1 2,285,793 - -0.7 2,285,793 - -0.5 2,285,793 - -0.2 2,285,793 - -0.5 2,285,793 - -0.9 2,285,793 - -0.5 2,285,793 - -1.0 2,285,793 - +1.1 2,285,793 - -2.0 2,285,793 - -0.8 2,285,793 - -0.5 2,285,794 + +0.3 2,285,794 + -1.2 2,285,794 + +1.7 2,285,794 + -0.9 2,285,794 + +0.1 2,285,795 - +1.1 2,285,798 + -0.2 2,285,798 + -0.9 2,285,798 + -0.2 2,285,798 + +0.3 2,285,798 + -0.5 2,285,798 + +0.3 2,285,798 + +2.6 2,285,799 - +0.3 2,285,799 - -0.5 2,285,799 - -0.5 2,285,799 - -2.0 2,285,799 - -1.5 2,285,799 - +0.1 2,285,799 - -1.2 2,285,799 - -0.3 2,285,809 + -0.2 2,285,810 - -2.3 2,285,810 - -1.7 2,285,891 - DZA65_RS10800 0.17 -1.3 2,285,940 - DZA65_RS10800 0.25 +0.8 2,285,969 + DZA65_RS10800 0.30 +0.1 2,285,969 + DZA65_RS10800 0.30 +2.1 2,285,969 + DZA65_RS10800 0.30 -0.5 2,285,969 + DZA65_RS10800 0.30 -0.6 2,285,969 + DZA65_RS10800 0.30 +1.0 2,285,969 + DZA65_RS10800 0.30 -2.0 2,285,969 + DZA65_RS10800 0.30 -1.0 2,285,970 - DZA65_RS10800 0.30 +0.1 2,285,970 - DZA65_RS10800 0.30 -0.1 2,285,970 - DZA65_RS10800 0.30 -0.1 2,285,970 - DZA65_RS10800 0.30 +1.9 2,285,970 - DZA65_RS10800 0.30 +0.7 2,285,971 + DZA65_RS10800 0.30 +0.5 2,285,971 + DZA65_RS10800 0.30 -1.3 2,285,971 + DZA65_RS10800 0.30 -1.0 2,285,972 - DZA65_RS10800 0.30 -0.9 2,285,972 - DZA65_RS10800 0.30 -0.9 2,285,972 - DZA65_RS10800 0.30 -1.5 2,285,972 - DZA65_RS10800 0.30 +0.4 2,285,972 - DZA65_RS10800 0.30 -0.5 2,285,972 - DZA65_RS10800 0.30 -0.7 2,285,975 + DZA65_RS10800 0.31 -1.9 2,285,975 + DZA65_RS10800 0.31 +1.1 2,285,975 + DZA65_RS10800 0.31 -0.5 2,285,976 - DZA65_RS10800 0.31 +0.6 2,285,976 - DZA65_RS10800 0.31 +1.5 2,285,976 - DZA65_RS10800 0.31 -0.9 2,285,976 - DZA65_RS10800 0.31 +0.1 2,285,976 - DZA65_RS10800 0.31 +1.7 2,285,976 - DZA65_RS10800 0.31 -0.5 2,286,079 + DZA65_RS10800 0.48 -1.3 2,286,079 + DZA65_RS10800 0.48 -3.5 2,286,080 - DZA65_RS10800 0.48 +0.5 2,286,080 - DZA65_RS10800 0.48 +1.6 2,286,080 - DZA65_RS10800 0.48 +1.1 2,286,080 - DZA65_RS10800 0.48 +0.0 2,286,086 - DZA65_RS10800 0.49 -1.2 2,286,148 + DZA65_RS10800 0.59 +0.9 2,286,148 + DZA65_RS10800 0.59 +0.5 2,286,148 + DZA65_RS10800 0.59 -0.9 2,286,148 + DZA65_RS10800 0.59 -1.9 2,286,158 - DZA65_RS10800 0.60 +0.5 2,286,174 + DZA65_RS10800 0.63 -0.5 2,286,181 + DZA65_RS10800 0.64 -3.0 2,286,181 + DZA65_RS10800 0.64 -1.7 2,286,224 + DZA65_RS10800 0.71 -0.9 2,286,224 + DZA65_RS10800 0.71 -0.9 2,286,224 + DZA65_RS10800 0.71 -1.9 2,286,224 + DZA65_RS10800 0.71 -0.7 2,286,224 + DZA65_RS10800 0.71 -0.5 2,286,224 + DZA65_RS10800 0.71 +1.1 2,286,225 - DZA65_RS10800 0.71 +2.1 2,286,225 - DZA65_RS10800 0.71 +1.1 2,286,225 - +0.1 2,286,294 + DZA65_RS10800 0.82 -1.2 2,286,295 - DZA65_RS10800 0.82 +0.1 2,286,325 - DZA65_RS10800 0.87 -0.5 2,286,377 + +0.5 2,286,377 + +0.6 2,286,377 + -1.2 2,286,377 + -2.3 2,286,377 + +2.7 2,286,377 + +1.1 2,286,377 + -0.2 2,286,377 + +1.5 2,286,377 + -1.7 2,286,378 - -1.0 2,286,378 - -0.5 2,286,378 - +0.5 2,286,378 - -2.4 2,286,378 - +1.1 2,286,378 - -0.5 2,286,378 - +2.8 2,286,379 + -0.9 2,286,379 + +1.5 2,286,379 + -1.5 2,286,380 - -0.2 2,286,380 - +0.8 2,286,380 - -2.0 2,286,380 - +0.3 2,286,380 - -1.5 2,286,401 + +0.1 2,286,401 + -1.7 2,286,402 - -1.5 2,286,421 + -1.5 2,286,422 - +1.1
Or see this region's nucleotide sequence