Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS08970 and DZA65_RS08975 overlap by 14 nucleotides DZA65_RS08975 and DZA65_RS08980 overlap by 8 nucleotides DZA65_RS08980 and DZA65_RS08990 are separated by 733 nucleotides
DZA65_RS08970: DZA65_RS08970 - 8-amino-7-oxononanoate synthase, at 1,890,026 to 1,891,180
_RS08970
DZA65_RS08975: DZA65_RS08975 - malonyl-ACP O-methyltransferase BioC, at 1,891,167 to 1,891,949
_RS08975
DZA65_RS08980: DZA65_RS08980 - ATP-dependent dethiobiotin synthetase BioD, at 1,891,942 to 1,892,622
_RS08980
DZA65_RS08990: DZA65_RS08990 - excinuclease ABC subunit B, at 1,893,356 to 1,895,368
_RS08990
Position (kb)
1891
1892
1893 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1891.111 kb on + strand at 1891.112 kb on - strand at 1891.112 kb on - strand at 1891.112 kb on - strand at 1891.112 kb on - strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.166 kb on + strand at 1891.167 kb on - strand at 1891.167 kb on - strand at 1891.167 kb on - strand at 1891.167 kb on - strand at 1891.167 kb on - strand at 1891.208 kb on + strand at 1891.208 kb on + strand at 1891.208 kb on + strand at 1891.209 kb on - strand at 1891.209 kb on - strand at 1891.251 kb on + strand, within DZA65_RS08975 at 1891.251 kb on + strand, within DZA65_RS08975 at 1891.251 kb on + strand, within DZA65_RS08975 at 1891.408 kb on + strand, within DZA65_RS08975 at 1891.409 kb on - strand, within DZA65_RS08975 at 1891.458 kb on + strand, within DZA65_RS08975 at 1891.458 kb on + strand, within DZA65_RS08975 at 1891.458 kb on + strand, within DZA65_RS08975 at 1891.676 kb on + strand at 1891.676 kb on + strand, within DZA65_RS08975 at 1891.676 kb on + strand, within DZA65_RS08975 at 1891.677 kb on - strand, within DZA65_RS08975 at 1891.679 kb on + strand, within DZA65_RS08975 at 1891.680 kb on - strand, within DZA65_RS08975 at 1891.761 kb on + strand, within DZA65_RS08975 at 1891.761 kb on + strand, within DZA65_RS08975 at 1891.761 kb on + strand, within DZA65_RS08975 at 1891.761 kb on + strand, within DZA65_RS08975 at 1891.762 kb on - strand, within DZA65_RS08975 at 1891.762 kb on - strand, within DZA65_RS08975 at 1891.762 kb on - strand, within DZA65_RS08975 at 1891.762 kb on - strand at 1891.881 kb on + strand at 1891.882 kb on - strand at 1891.902 kb on + strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.914 kb on + strand at 1891.915 kb on - strand at 1891.915 kb on - strand at 1891.915 kb on - strand at 1891.915 kb on - strand at 1891.915 kb on - strand at 1891.915 kb on - strand at 1891.915 kb on - strand at 1891.982 kb on + strand at 1891.982 kb on + strand at 1891.983 kb on - strand at 1891.983 kb on - strand at 1891.983 kb on - strand at 1892.051 kb on - strand, within DZA65_RS08980 at 1892.053 kb on - strand, within DZA65_RS08980 at 1892.194 kb on - strand, within DZA65_RS08980 at 1892.194 kb on - strand, within DZA65_RS08980 at 1892.194 kb on - strand, within DZA65_RS08980 at 1892.328 kb on + strand, within DZA65_RS08980 at 1892.328 kb on + strand, within DZA65_RS08980 at 1892.328 kb on + strand, within DZA65_RS08980 at 1892.328 kb on + strand, within DZA65_RS08980 at 1892.328 kb on + strand, within DZA65_RS08980 at 1892.328 kb on + strand, within DZA65_RS08980 at 1892.328 kb on + strand, within DZA65_RS08980 at 1892.329 kb on - strand, within DZA65_RS08980 at 1892.329 kb on - strand, within DZA65_RS08980 at 1892.329 kb on - strand, within DZA65_RS08980 at 1892.329 kb on - strand, within DZA65_RS08980 at 1892.329 kb on - strand, within DZA65_RS08980 at 1892.504 kb on - strand, within DZA65_RS08980 at 1892.593 kb on + strand at 1892.593 kb on + strand at 1892.594 kb on - strand at 1892.597 kb on + strand at 1892.597 kb on + strand at 1892.598 kb on - strand at 1892.598 kb on - strand at 1892.743 kb on + strand at 1892.743 kb on + strand at 1892.744 kb on - strand at 1892.807 kb on + strand at 1892.807 kb on + strand at 1892.818 kb on + strand at 1892.819 kb on - strand at 1892.857 kb on + strand at 1892.857 kb on + strand at 1892.905 kb on - strand at 1892.908 kb on + strand at 1892.908 kb on + strand at 1892.908 kb on + strand at 1892.908 kb on + strand at 1892.909 kb on - strand at 1892.909 kb on - strand at 1892.914 kb on + strand at 1892.914 kb on + strand at 1892.915 kb on - strand at 1892.915 kb on - strand at 1892.915 kb on - strand at 1892.917 kb on + strand at 1892.923 kb on + strand at 1893.037 kb on + strand at 1893.037 kb on + strand at 1893.038 kb on - strand at 1893.060 kb on + strand at 1893.061 kb on - strand at 1893.080 kb on + strand at 1893.081 kb on - strand at 1893.118 kb on + strand at 1893.118 kb on + strand at 1893.118 kb on + strand at 1893.118 kb on + strand at 1893.118 kb on + strand at 1893.118 kb on + strand at 1893.118 kb on + strand at 1893.119 kb on - strand at 1893.119 kb on - strand at 1893.119 kb on - strand at 1893.119 kb on - strand at 1893.119 kb on - strand at 1893.119 kb on - strand at 1893.138 kb on + strand at 1893.195 kb on - strand at 1893.262 kb on + strand at 1893.262 kb on + strand at 1893.266 kb on - strand at 1893.266 kb on - strand at 1893.266 kb on - strand at 1893.266 kb on - strand at 1893.266 kb on - strand at 1893.355 kb on + strand at 1893.356 kb on - strand at 1893.356 kb on - strand at 1893.356 kb on - strand at 1893.356 kb on - strand at 1893.356 kb on - strand at 1893.361 kb on + strand at 1893.361 kb on + strand at 1893.362 kb on - strand at 1893.362 kb on - strand at 1893.362 kb on - strand at 1893.362 kb on - strand at 1893.362 kb on - strand at 1893.362 kb on - strand at 1893.417 kb on + strand at 1893.418 kb on - strand at 1893.487 kb on + strand at 1893.575 kb on + strand, within DZA65_RS08990 at 1893.575 kb on + strand, within DZA65_RS08990 at 1893.575 kb on + strand, within DZA65_RS08990 at 1893.576 kb on - strand, within DZA65_RS08990 at 1893.618 kb on - strand, within DZA65_RS08990 at 1893.618 kb on - strand, within DZA65_RS08990 at 1893.618 kb on - strand, within DZA65_RS08990 at 1893.618 kb on - strand, within DZA65_RS08990
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 1,891,111 + -0.3 1,891,112 - -0.6 1,891,112 - +0.1 1,891,112 - +0.2 1,891,112 - -0.3 1,891,166 + +0.7 1,891,166 + -0.8 1,891,166 + -2.3 1,891,166 + +0.2 1,891,166 + +3.0 1,891,166 + +0.7 1,891,166 + +1.0 1,891,166 + +2.0 1,891,166 + +2.2 1,891,167 - -1.3 1,891,167 - -0.8 1,891,167 - +1.2 1,891,167 - +0.1 1,891,167 - +0.5 1,891,208 + -0.4 1,891,208 + +1.2 1,891,208 + +1.0 1,891,209 - +0.2 1,891,209 - +2.0 1,891,251 + DZA65_RS08975 0.11 -1.0 1,891,251 + DZA65_RS08975 0.11 +3.2 1,891,251 + DZA65_RS08975 0.11 -0.3 1,891,408 + DZA65_RS08975 0.31 -2.3 1,891,409 - DZA65_RS08975 0.31 +0.8 1,891,458 + DZA65_RS08975 0.37 -0.8 1,891,458 + DZA65_RS08975 0.37 -0.3 1,891,458 + DZA65_RS08975 0.37 -0.1 1,891,676 + -0.9 1,891,676 + DZA65_RS08975 0.65 -1.1 1,891,676 + DZA65_RS08975 0.65 -1.6 1,891,677 - DZA65_RS08975 0.65 -0.1 1,891,679 + DZA65_RS08975 0.65 -0.2 1,891,680 - DZA65_RS08975 0.66 -1.6 1,891,761 + DZA65_RS08975 0.76 -1.8 1,891,761 + DZA65_RS08975 0.76 -0.3 1,891,761 + DZA65_RS08975 0.76 +0.2 1,891,761 + DZA65_RS08975 0.76 -0.8 1,891,762 - DZA65_RS08975 0.76 +0.2 1,891,762 - DZA65_RS08975 0.76 -0.5 1,891,762 - DZA65_RS08975 0.76 -0.9 1,891,762 - -0.6 1,891,881 + -1.5 1,891,882 - -1.5 1,891,902 + -0.9 1,891,914 + +4.2 1,891,914 + +2.2 1,891,914 + +0.2 1,891,914 + +0.2 1,891,914 + -0.3 1,891,914 + -0.5 1,891,915 - +0.2 1,891,915 - -2.6 1,891,915 - +1.2 1,891,915 - -1.8 1,891,915 - -0.3 1,891,915 - +2.0 1,891,915 - -1.1 1,891,982 + +0.0 1,891,982 + -1.1 1,891,983 - -0.8 1,891,983 - +2.7 1,891,983 - -1.6 1,892,051 - DZA65_RS08980 0.16 -0.4 1,892,053 - DZA65_RS08980 0.16 -0.8 1,892,194 - DZA65_RS08980 0.37 -0.8 1,892,194 - DZA65_RS08980 0.37 -0.8 1,892,194 - DZA65_RS08980 0.37 -2.6 1,892,328 + DZA65_RS08980 0.57 +0.2 1,892,328 + DZA65_RS08980 0.57 +0.9 1,892,328 + DZA65_RS08980 0.57 +0.2 1,892,328 + DZA65_RS08980 0.57 +1.2 1,892,328 + DZA65_RS08980 0.57 -1.6 1,892,328 + DZA65_RS08980 0.57 +0.2 1,892,328 + DZA65_RS08980 0.57 -0.8 1,892,329 - DZA65_RS08980 0.57 +0.8 1,892,329 - DZA65_RS08980 0.57 +0.9 1,892,329 - DZA65_RS08980 0.57 +0.2 1,892,329 - DZA65_RS08980 0.57 -0.3 1,892,329 - DZA65_RS08980 0.57 +0.2 1,892,504 - DZA65_RS08980 0.83 -1.8 1,892,593 + -0.8 1,892,593 + +0.2 1,892,594 - -1.9 1,892,597 + +0.2 1,892,597 + +0.2 1,892,598 - -0.8 1,892,598 - +0.2 1,892,743 + -1.4 1,892,743 + -0.4 1,892,744 - -0.9 1,892,807 + +2.0 1,892,807 + -0.1 1,892,818 + +0.2 1,892,819 - +0.1 1,892,857 + -1.2 1,892,857 + -1.9 1,892,905 - -0.8 1,892,908 + +2.0 1,892,908 + +0.2 1,892,908 + -0.1 1,892,908 + +1.3 1,892,909 - +1.2 1,892,909 - -2.1 1,892,914 + -0.2 1,892,914 + +0.6 1,892,915 - -1.6 1,892,915 - +0.5 1,892,915 - -0.6 1,892,917 + -0.4 1,892,923 + +2.2 1,893,037 + -1.1 1,893,037 + -0.6 1,893,038 - +1.6 1,893,060 + +2.2 1,893,061 - +1.1 1,893,080 + -0.4 1,893,081 - +0.2 1,893,118 + -1.6 1,893,118 + +0.9 1,893,118 + +1.0 1,893,118 + +0.2 1,893,118 + -0.1 1,893,118 + -0.6 1,893,118 + -0.9 1,893,119 - +0.7 1,893,119 - -1.1 1,893,119 - -0.6 1,893,119 - -0.4 1,893,119 - -0.0 1,893,119 - +1.3 1,893,138 + -1.6 1,893,195 - -0.4 1,893,262 + -1.1 1,893,262 + +0.2 1,893,266 - -0.2 1,893,266 - -1.1 1,893,266 - -0.5 1,893,266 - +0.4 1,893,266 - -2.1 1,893,355 + -1.0 1,893,356 - +0.2 1,893,356 - -0.5 1,893,356 - -1.5 1,893,356 - -1.4 1,893,356 - +1.0 1,893,361 + +0.9 1,893,361 + -0.1 1,893,362 - -2.7 1,893,362 - -0.7 1,893,362 - -1.2 1,893,362 - -0.6 1,893,362 - -0.5 1,893,362 - -0.1 1,893,417 + +1.2 1,893,418 - -3.6 1,893,487 + -0.4 1,893,575 + DZA65_RS08990 0.11 -0.4 1,893,575 + DZA65_RS08990 0.11 -0.1 1,893,575 + DZA65_RS08990 0.11 +0.2 1,893,576 - DZA65_RS08990 0.11 +1.6 1,893,618 - DZA65_RS08990 0.13 -1.7 1,893,618 - DZA65_RS08990 0.13 -2.2 1,893,618 - DZA65_RS08990 0.13 +0.2 1,893,618 - DZA65_RS08990 0.13 -2.6
Or see this region's nucleotide sequence