Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS00990 and DZA65_RS00995 are separated by 31 nucleotides DZA65_RS00995 and DZA65_RS01000 are separated by 212 nucleotides DZA65_RS01000 and DZA65_RS01005 are separated by 49 nucleotides DZA65_RS01005 and DZA65_RS01010 are separated by 94 nucleotides
DZA65_RS00990: DZA65_RS00990 - porphobilinogen synthase, at 225,284 to 226,306
_RS00990
DZA65_RS00995: DZA65_RS00995 - transcription/translation regulatory transformer protein RfaH, at 226,338 to 226,826
_RS00995
DZA65_RS01000: DZA65_RS01000 - 4-hydroxy-3-polyprenylbenzoate decarboxylase, at 227,039 to 228,526
_RS01000
DZA65_RS01005: DZA65_RS01005 - NAD(P)H-flavin reductase, at 228,576 to 229,277
_RS01005
DZA65_RS01010: DZA65_RS01010 - FAD-dependent oxidoreductase, at 229,372 to 231,045
_RS01010
Position (kb) 227
228
229 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 226.310 kb on + strand at 226.310 kb on + strand at 226.310 kb on + strand at 226.311 kb on - strand at 226.311 kb on - strand at 226.311 kb on - strand at 226.311 kb on - strand at 226.311 kb on - strand at 226.311 kb on - strand at 226.311 kb on - strand at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.438 kb on + strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.439 kb on - strand, within DZA65_RS00995 at 226.442 kb on + strand, within DZA65_RS00995 at 226.442 kb on + strand, within DZA65_RS00995 at 226.443 kb on - strand, within DZA65_RS00995 at 226.479 kb on + strand, within DZA65_RS00995 at 226.480 kb on - strand, within DZA65_RS00995 at 226.514 kb on + strand, within DZA65_RS00995 at 226.514 kb on + strand, within DZA65_RS00995 at 226.515 kb on - strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.635 kb on + strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.636 kb on - strand, within DZA65_RS00995 at 226.666 kb on + strand, within DZA65_RS00995 at 226.666 kb on + strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.667 kb on - strand, within DZA65_RS00995 at 226.711 kb on - strand, within DZA65_RS00995 at 226.783 kb on - strand at 226.804 kb on + strand at 226.804 kb on + strand at 226.804 kb on + strand at 226.805 kb on - strand at 226.809 kb on + strand at 226.809 kb on + strand at 226.809 kb on + strand at 226.809 kb on + strand at 226.809 kb on + strand at 226.809 kb on + strand at 226.810 kb on - strand at 226.810 kb on - strand at 226.810 kb on - strand at 226.810 kb on - strand at 226.810 kb on - strand at 226.810 kb on - strand at 226.813 kb on + strand at 226.814 kb on - strand at 226.826 kb on + strand at 226.827 kb on - strand at 226.827 kb on - strand at 226.876 kb on + strand at 226.877 kb on - strand at 226.877 kb on - strand at 226.963 kb on + strand at 226.963 kb on + strand at 226.964 kb on - strand at 226.964 kb on - strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.001 kb on + strand at 227.002 kb on - strand at 227.002 kb on - strand at 227.003 kb on + strand at 227.003 kb on + strand at 227.003 kb on + strand at 227.003 kb on + strand at 227.003 kb on + strand at 227.003 kb on + strand at 228.478 kb on + strand at 228.534 kb on + strand at 228.562 kb on + strand at 228.562 kb on + strand at 228.593 kb on + strand at 228.593 kb on + strand at 228.599 kb on - strand at 228.792 kb on + strand, within DZA65_RS01005 at 228.924 kb on + strand, within DZA65_RS01005 at 228.928 kb on + strand, within DZA65_RS01005 at 228.928 kb on + strand, within DZA65_RS01005 at 228.976 kb on + strand, within DZA65_RS01005 at 228.976 kb on + strand, within DZA65_RS01005 at 228.977 kb on - strand, within DZA65_RS01005 at 229.167 kb on - strand, within DZA65_RS01005 at 229.170 kb on + strand, within DZA65_RS01005 at 229.171 kb on - strand, within DZA65_RS01005 at 229.171 kb on - strand, within DZA65_RS01005 at 229.171 kb on - strand, within DZA65_RS01005 at 229.171 kb on - strand, within DZA65_RS01005 at 229.251 kb on + strand at 229.272 kb on - strand at 229.272 kb on - strand at 229.328 kb on - strand at 229.330 kb on - strand at 229.360 kb on + strand at 229.361 kb on - strand at 229.373 kb on + strand at 229.373 kb on + strand at 229.374 kb on - strand at 229.374 kb on - strand at 229.374 kb on - strand at 229.402 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 226,310 + +0.0 226,310 + -1.7 226,310 + +0.0 226,311 - -1.3 226,311 - +1.2 226,311 - -0.7 226,311 - +1.6 226,311 - -1.0 226,311 - -0.4 226,311 - -1.1 226,438 + DZA65_RS00995 0.20 +0.9 226,438 + DZA65_RS00995 0.20 -1.9 226,438 + DZA65_RS00995 0.20 -1.4 226,438 + DZA65_RS00995 0.20 -0.4 226,438 + DZA65_RS00995 0.20 -0.8 226,438 + DZA65_RS00995 0.20 +0.6 226,438 + DZA65_RS00995 0.20 -2.2 226,438 + DZA65_RS00995 0.20 -2.0 226,438 + DZA65_RS00995 0.20 -0.4 226,438 + DZA65_RS00995 0.20 -1.4 226,438 + DZA65_RS00995 0.20 -1.6 226,438 + DZA65_RS00995 0.20 -1.1 226,438 + DZA65_RS00995 0.20 -1.7 226,438 + DZA65_RS00995 0.20 +0.0 226,438 + DZA65_RS00995 0.20 -2.2 226,438 + DZA65_RS00995 0.20 +1.1 226,438 + DZA65_RS00995 0.20 +0.2 226,438 + DZA65_RS00995 0.20 +0.0 226,439 - DZA65_RS00995 0.21 -1.2 226,439 - DZA65_RS00995 0.21 +0.9 226,439 - DZA65_RS00995 0.21 +0.0 226,439 - DZA65_RS00995 0.21 -2.1 226,439 - DZA65_RS00995 0.21 -1.8 226,439 - DZA65_RS00995 0.21 -1.8 226,439 - DZA65_RS00995 0.21 -1.2 226,439 - DZA65_RS00995 0.21 -1.7 226,439 - DZA65_RS00995 0.21 -1.6 226,439 - DZA65_RS00995 0.21 +0.1 226,439 - DZA65_RS00995 0.21 -2.8 226,442 + DZA65_RS00995 0.21 +0.8 226,442 + DZA65_RS00995 0.21 -1.2 226,443 - DZA65_RS00995 0.21 +2.0 226,479 + DZA65_RS00995 0.29 -1.2 226,480 - DZA65_RS00995 0.29 -1.6 226,514 + DZA65_RS00995 0.36 -0.7 226,514 + DZA65_RS00995 0.36 +0.6 226,515 - DZA65_RS00995 0.36 +0.3 226,635 + DZA65_RS00995 0.61 -2.0 226,635 + DZA65_RS00995 0.61 -0.2 226,635 + DZA65_RS00995 0.61 +0.0 226,635 + DZA65_RS00995 0.61 -0.6 226,635 + DZA65_RS00995 0.61 -0.7 226,635 + DZA65_RS00995 0.61 -1.4 226,635 + DZA65_RS00995 0.61 -2.1 226,635 + DZA65_RS00995 0.61 -2.1 226,635 + DZA65_RS00995 0.61 -2.0 226,635 + DZA65_RS00995 0.61 -1.4 226,635 + DZA65_RS00995 0.61 -0.7 226,635 + DZA65_RS00995 0.61 -1.0 226,635 + DZA65_RS00995 0.61 +0.8 226,636 - DZA65_RS00995 0.61 -1.0 226,636 - DZA65_RS00995 0.61 -0.7 226,636 - DZA65_RS00995 0.61 -0.5 226,636 - DZA65_RS00995 0.61 -0.0 226,636 - DZA65_RS00995 0.61 -0.4 226,636 - DZA65_RS00995 0.61 -1.4 226,636 - DZA65_RS00995 0.61 -2.1 226,636 - DZA65_RS00995 0.61 +2.1 226,636 - DZA65_RS00995 0.61 +0.4 226,636 - DZA65_RS00995 0.61 -0.2 226,636 - DZA65_RS00995 0.61 +1.6 226,636 - DZA65_RS00995 0.61 +0.0 226,636 - DZA65_RS00995 0.61 -0.4 226,636 - DZA65_RS00995 0.61 -0.4 226,636 - DZA65_RS00995 0.61 +0.0 226,666 + DZA65_RS00995 0.67 -2.2 226,666 + DZA65_RS00995 0.67 +0.8 226,667 - DZA65_RS00995 0.67 +0.6 226,667 - DZA65_RS00995 0.67 +1.6 226,667 - DZA65_RS00995 0.67 -0.6 226,667 - DZA65_RS00995 0.67 +1.3 226,667 - DZA65_RS00995 0.67 -2.6 226,667 - DZA65_RS00995 0.67 +0.7 226,667 - DZA65_RS00995 0.67 +0.3 226,667 - DZA65_RS00995 0.67 -0.4 226,711 - DZA65_RS00995 0.76 +0.0 226,783 - +4.2 226,804 + +1.6 226,804 + -0.7 226,804 + +0.1 226,805 - +0.6 226,809 + -2.3 226,809 + -0.8 226,809 + +0.0 226,809 + -1.8 226,809 + -1.2 226,809 + -1.4 226,810 - +0.6 226,810 - -0.7 226,810 - -2.4 226,810 - -1.4 226,810 - -1.2 226,810 - +0.9 226,813 + -1.4 226,814 - +2.5 226,826 + +0.8 226,827 - +0.9 226,827 - +0.7 226,876 + +0.0 226,877 - -0.4 226,877 - -1.6 226,963 + +0.5 226,963 + -0.1 226,964 - -2.1 226,964 - +0.5 227,001 + -1.5 227,001 + -0.7 227,001 + -1.0 227,001 + -0.2 227,001 + +0.0 227,001 + -1.4 227,001 + +0.9 227,001 + +1.3 227,001 + -1.4 227,002 - +0.9 227,002 - -0.8 227,003 + +0.0 227,003 + +0.0 227,003 + -1.0 227,003 + -0.3 227,003 + -0.4 227,003 + -2.2 228,478 + +0.0 228,534 + -3.6 228,562 + +1.0 228,562 + -1.8 228,593 + -1.0 228,593 + -0.7 228,599 - -0.7 228,792 + DZA65_RS01005 0.31 -1.2 228,924 + DZA65_RS01005 0.50 -1.2 228,928 + DZA65_RS01005 0.50 +0.0 228,928 + DZA65_RS01005 0.50 -0.8 228,976 + DZA65_RS01005 0.57 -1.0 228,976 + DZA65_RS01005 0.57 +1.2 228,977 - DZA65_RS01005 0.57 -0.4 229,167 - DZA65_RS01005 0.84 -0.4 229,170 + DZA65_RS01005 0.85 -1.0 229,171 - DZA65_RS01005 0.85 -2.3 229,171 - DZA65_RS01005 0.85 +0.6 229,171 - DZA65_RS01005 0.85 +1.0 229,171 - DZA65_RS01005 0.85 -0.4 229,251 + -1.4 229,272 - -1.9 229,272 - -0.7 229,328 - +0.1 229,330 - -2.3 229,360 + +0.6 229,361 - -0.2 229,373 + -1.6 229,373 + -0.7 229,374 - -0.4 229,374 - -0.2 229,374 - +2.4 229,402 - +1.6
Or see this region's nucleotide sequence