Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS04215 and DZA65_RS04220 are separated by 155 nucleotides DZA65_RS04220 and DZA65_RS04225 overlap by 8 nucleotides DZA65_RS04225 and DZA65_RS04230 are separated by 58 nucleotides DZA65_RS04230 and DZA65_RS04235 are separated by 111 nucleotides
DZA65_RS04215: DZA65_RS04215 - SsrA-binding protein SmpB, at 881,253 to 881,735
_RS04215
DZA65_RS04220: DZA65_RS04220 - type II toxin-antitoxin system RatA family toxin, at 881,891 to 882,325
_RS04220
DZA65_RS04225: DZA65_RS04225 - RnfH family protein, at 882,318 to 882,611
_RS04225
DZA65_RS04230: DZA65_RS04230 - outer membrane protein assembly factor BamE, at 882,670 to 883,014
_RS04230
DZA65_RS04235: DZA65_RS04235 - DNA repair protein RecN, at 883,126 to 884,787
_RS04235
Position (kb) 881
882
883 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 880.896 kb on + strand at 880.897 kb on - strand at 881.219 kb on - strand at 881.220 kb on + strand at 881.220 kb on + strand at 881.221 kb on - strand at 881.239 kb on + strand at 881.240 kb on - strand at 881.244 kb on + strand at 881.245 kb on - strand at 881.245 kb on - strand at 881.245 kb on - strand at 881.245 kb on - strand at 881.245 kb on - strand at 881.410 kb on + strand, within DZA65_RS04215 at 881.410 kb on + strand, within DZA65_RS04215 at 881.411 kb on - strand, within DZA65_RS04215 at 881.471 kb on + strand, within DZA65_RS04215 at 881.572 kb on + strand, within DZA65_RS04215 at 881.572 kb on + strand, within DZA65_RS04215 at 881.573 kb on - strand, within DZA65_RS04215 at 881.573 kb on - strand, within DZA65_RS04215 at 881.666 kb on - strand, within DZA65_RS04215 at 881.666 kb on - strand, within DZA65_RS04215 at 881.666 kb on - strand, within DZA65_RS04215 at 881.714 kb on + strand at 881.747 kb on + strand at 881.748 kb on - strand at 881.748 kb on - strand at 881.748 kb on - strand at 881.748 kb on - strand at 881.749 kb on + strand at 881.749 kb on + strand at 881.750 kb on - strand at 881.750 kb on - strand at 881.791 kb on - strand at 881.791 kb on - strand at 881.854 kb on + strand at 881.857 kb on + strand at 881.857 kb on + strand at 881.857 kb on + strand at 881.858 kb on - strand at 881.858 kb on - strand at 881.858 kb on - strand at 881.858 kb on - strand at 881.872 kb on - strand at 881.890 kb on + strand at 881.891 kb on - strand at 881.943 kb on - strand, within DZA65_RS04220 at 881.945 kb on - strand, within DZA65_RS04220 at 881.945 kb on - strand, within DZA65_RS04220 at 881.945 kb on - strand at 882.089 kb on - strand, within DZA65_RS04220 at 882.109 kb on + strand, within DZA65_RS04220 at 882.167 kb on - strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.190 kb on + strand, within DZA65_RS04220 at 882.191 kb on - strand, within DZA65_RS04220 at 882.191 kb on - strand, within DZA65_RS04220 at 882.191 kb on - strand, within DZA65_RS04220 at 882.277 kb on + strand, within DZA65_RS04220 at 882.277 kb on + strand, within DZA65_RS04220 at 882.314 kb on + strand at 882.314 kb on + strand at 882.314 kb on + strand at 882.315 kb on - strand at 882.315 kb on - strand at 882.346 kb on - strand at 882.366 kb on + strand at 882.367 kb on - strand, within DZA65_RS04225 at 882.414 kb on - strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.508 kb on + strand, within DZA65_RS04225 at 882.509 kb on - strand, within DZA65_RS04225 at 882.509 kb on - strand, within DZA65_RS04225 at 882.509 kb on - strand, within DZA65_RS04225 at 882.560 kb on + strand, within DZA65_RS04225 at 882.568 kb on + strand, within DZA65_RS04225 at 882.569 kb on - strand, within DZA65_RS04225 at 882.671 kb on + strand at 882.671 kb on + strand at 882.671 kb on + strand at 882.671 kb on + strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.672 kb on - strand at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.788 kb on + strand, within DZA65_RS04230 at 882.789 kb on - strand, within DZA65_RS04230 at 882.789 kb on - strand at 882.842 kb on + strand, within DZA65_RS04230 at 882.842 kb on + strand, within DZA65_RS04230 at 882.842 kb on + strand, within DZA65_RS04230 at 882.842 kb on + strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.843 kb on - strand, within DZA65_RS04230 at 882.883 kb on + strand, within DZA65_RS04230 at 882.884 kb on - strand, within DZA65_RS04230 at 882.902 kb on + strand, within DZA65_RS04230 at 882.902 kb on + strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.903 kb on - strand, within DZA65_RS04230 at 882.934 kb on + strand, within DZA65_RS04230 at 882.934 kb on + strand, within DZA65_RS04230 at 882.934 kb on + strand, within DZA65_RS04230 at 882.935 kb on - strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.969 kb on + strand, within DZA65_RS04230 at 882.970 kb on - strand, within DZA65_RS04230 at 882.970 kb on - strand, within DZA65_RS04230 at 882.970 kb on - strand, within DZA65_RS04230 at 883.005 kb on + strand at 883.014 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.021 kb on + strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.022 kb on - strand at 883.038 kb on + strand at 883.038 kb on + strand at 883.038 kb on + strand at 883.039 kb on - strand at 883.052 kb on + strand at 883.052 kb on + strand at 883.052 kb on + strand at 883.052 kb on + strand at 883.053 kb on - strand at 883.053 kb on - strand at 883.053 kb on - strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.055 kb on + strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.056 kb on - strand at 883.093 kb on - strand at 883.127 kb on + strand at 883.128 kb on - strand at 883.269 kb on + strand at 883.270 kb on - strand at 883.280 kb on + strand at 883.280 kb on + strand at 883.280 kb on + strand at 883.280 kb on + strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand at 883.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 880,896 + -1.5 880,897 - -1.2 881,219 - -1.2 881,220 + -0.5 881,220 + -3.9 881,221 - -1.7 881,239 + +0.5 881,240 - -1.5 881,244 + +1.1 881,245 - -1.7 881,245 - +0.1 881,245 - +1.6 881,245 - -1.5 881,245 - -0.5 881,410 + DZA65_RS04215 0.33 -3.1 881,410 + DZA65_RS04215 0.33 -0.9 881,411 - DZA65_RS04215 0.33 -0.9 881,471 + DZA65_RS04215 0.45 +0.9 881,572 + DZA65_RS04215 0.66 -0.9 881,572 + DZA65_RS04215 0.66 +1.3 881,573 - DZA65_RS04215 0.66 -2.0 881,573 - DZA65_RS04215 0.66 +2.1 881,666 - DZA65_RS04215 0.86 -0.9 881,666 - DZA65_RS04215 0.86 -0.5 881,666 - DZA65_RS04215 0.86 -0.9 881,714 + -1.5 881,747 + -1.5 881,748 - +0.5 881,748 - +1.3 881,748 - -0.1 881,748 - -0.6 881,749 + -1.2 881,749 + -2.5 881,750 - -0.5 881,750 - -0.4 881,791 - -1.5 881,791 - -1.2 881,854 + -3.1 881,857 + -3.0 881,857 + -0.9 881,857 + +0.1 881,858 - +0.1 881,858 - -0.9 881,858 - -0.0 881,858 - -1.7 881,872 - -0.2 881,890 + -0.6 881,891 - -0.5 881,943 - DZA65_RS04220 0.12 +1.1 881,945 - DZA65_RS04220 0.12 -0.5 881,945 - DZA65_RS04220 0.12 -0.9 881,945 - -0.9 882,089 - DZA65_RS04220 0.46 -1.7 882,109 + DZA65_RS04220 0.50 -2.0 882,167 - DZA65_RS04220 0.63 +2.7 882,190 + DZA65_RS04220 0.69 -0.4 882,190 + DZA65_RS04220 0.69 -1.9 882,190 + DZA65_RS04220 0.69 +1.1 882,190 + DZA65_RS04220 0.69 +0.0 882,191 - DZA65_RS04220 0.69 -0.2 882,191 - DZA65_RS04220 0.69 -0.4 882,191 - DZA65_RS04220 0.69 -1.7 882,277 + DZA65_RS04220 0.89 -0.9 882,277 + DZA65_RS04220 0.89 -0.0 882,314 + -2.3 882,314 + -0.9 882,314 + -1.9 882,315 - +1.1 882,315 - +1.4 882,346 - -1.7 882,366 + -0.3 882,367 - DZA65_RS04225 0.17 -1.5 882,414 - DZA65_RS04225 0.33 +0.4 882,508 + DZA65_RS04225 0.65 +1.9 882,508 + DZA65_RS04225 0.65 -2.0 882,508 + DZA65_RS04225 0.65 -0.6 882,508 + DZA65_RS04225 0.65 +1.1 882,508 + DZA65_RS04225 0.65 -0.3 882,509 - DZA65_RS04225 0.65 +0.5 882,509 - DZA65_RS04225 0.65 +0.1 882,509 - DZA65_RS04225 0.65 +0.9 882,560 + DZA65_RS04225 0.82 +1.3 882,568 + DZA65_RS04225 0.85 -2.7 882,569 - DZA65_RS04225 0.85 +1.1 882,671 + -0.3 882,671 + +1.4 882,671 + -1.2 882,671 + -2.5 882,672 - -0.6 882,672 - -1.5 882,672 - -0.5 882,672 - +1.3 882,672 - +1.1 882,672 - +1.9 882,788 + DZA65_RS04230 0.34 -1.7 882,788 + DZA65_RS04230 0.34 -3.6 882,788 + DZA65_RS04230 0.34 -0.9 882,788 + DZA65_RS04230 0.34 -0.5 882,788 + DZA65_RS04230 0.34 -2.0 882,788 + DZA65_RS04230 0.34 -1.7 882,788 + DZA65_RS04230 0.34 +3.3 882,789 - DZA65_RS04230 0.34 -1.2 882,789 - -0.7 882,842 + DZA65_RS04230 0.50 -2.7 882,842 + DZA65_RS04230 0.50 -1.6 882,842 + DZA65_RS04230 0.50 -2.2 882,842 + DZA65_RS04230 0.50 -0.0 882,843 - DZA65_RS04230 0.50 -0.5 882,843 - DZA65_RS04230 0.50 -1.9 882,843 - DZA65_RS04230 0.50 -0.6 882,843 - DZA65_RS04230 0.50 +3.3 882,843 - DZA65_RS04230 0.50 -0.1 882,883 + DZA65_RS04230 0.62 -2.3 882,884 - DZA65_RS04230 0.62 -1.7 882,902 + DZA65_RS04230 0.67 -2.0 882,902 + DZA65_RS04230 0.67 -0.9 882,903 - DZA65_RS04230 0.68 +1.1 882,903 - DZA65_RS04230 0.68 +2.1 882,903 - DZA65_RS04230 0.68 -2.8 882,903 - DZA65_RS04230 0.68 -0.5 882,903 - DZA65_RS04230 0.68 -1.7 882,934 + DZA65_RS04230 0.77 -0.9 882,934 + DZA65_RS04230 0.77 +1.1 882,934 + DZA65_RS04230 0.77 -2.2 882,935 - DZA65_RS04230 0.77 -0.7 882,969 + DZA65_RS04230 0.87 +0.1 882,969 + DZA65_RS04230 0.87 -1.6 882,969 + DZA65_RS04230 0.87 -0.6 882,969 + DZA65_RS04230 0.87 -2.6 882,969 + DZA65_RS04230 0.87 +0.4 882,969 + DZA65_RS04230 0.87 +1.1 882,969 + DZA65_RS04230 0.87 +1.9 882,969 + DZA65_RS04230 0.87 -2.5 882,970 - DZA65_RS04230 0.87 -2.5 882,970 - DZA65_RS04230 0.87 -1.3 882,970 - DZA65_RS04230 0.87 -1.2 883,005 + -1.2 883,014 + -2.2 883,021 + -0.9 883,021 + -1.5 883,021 + +0.3 883,021 + +1.8 883,021 + -1.2 883,021 + -2.1 883,021 + -1.2 883,021 + -2.1 883,022 - -0.3 883,022 - -0.9 883,022 - -1.2 883,022 - -1.7 883,022 - -2.2 883,022 - -0.5 883,022 - -2.3 883,038 + -0.5 883,038 + -0.5 883,038 + -1.6 883,039 - -0.9 883,052 + -2.6 883,052 + -3.0 883,052 + -2.3 883,052 + -1.0 883,053 - +1.1 883,053 - +1.9 883,053 - +0.1 883,055 + +0.1 883,055 + -2.5 883,055 + -1.2 883,055 + -1.9 883,055 + -2.2 883,056 - +1.3 883,056 - +1.1 883,056 - -3.3 883,056 - -1.7 883,056 - -0.5 883,056 - -0.9 883,056 - +2.4 883,056 - -0.6 883,056 - -1.5 883,056 - -2.0 883,093 - -0.5 883,127 + -2.2 883,128 - -2.5 883,269 + -1.2 883,270 - -1.2 883,280 + -1.7 883,280 + -1.3 883,280 + -1.0 883,280 + -2.1 883,281 - -2.9 883,281 - -1.7 883,281 - -0.5 883,281 - +0.1 883,281 - -2.6 883,281 - -1.7 883,281 - -0.9
Or see this region's nucleotide sequence