Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS19065 and DZA65_RS19070 are separated by 31 nucleotides DZA65_RS19070 and DZA65_RS19075 are separated by 84 nucleotides DZA65_RS19075 and DZA65_RS19080 are separated by 69 nucleotides DZA65_RS19080 and DZA65_RS19085 are separated by 97 nucleotides DZA65_RS19085 and DZA65_RS19090 are separated by 27 nucleotides
DZA65_RS19065: DZA65_RS19065 - cell division protein ZapD, at 4,121,737 to 4,122,486
_RS19065
DZA65_RS19070: DZA65_RS19070 - DNA gyrase inhibitor YacG, at 4,122,518 to 4,122,715
_RS19070
DZA65_RS19075: DZA65_RS19075 - 8-oxo-dGTP diphosphatase MutT, at 4,122,800 to 4,123,201
_RS19075
DZA65_RS19080: DZA65_RS19080 - preprotein translocase subunit SecA, at 4,123,271 to 4,125,964
_RS19080
DZA65_RS19085: DZA65_RS19085 - secA regulator SecM, at 4,126,062 to 4,126,562
_RS19085
DZA65_RS19090: DZA65_RS19090 - DUF721 domain-containing protein, at 4,126,590 to 4,127,111
_RS19090
Position (kb)
4123
4124
4125
4126 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4122.316 kb on + strand, within DZA65_RS19065 at 4122.316 kb on + strand, within DZA65_RS19065 at 4122.317 kb on - strand, within DZA65_RS19065 at 4122.317 kb on - strand, within DZA65_RS19065 at 4122.324 kb on + strand, within DZA65_RS19065 at 4122.325 kb on - strand, within DZA65_RS19065 at 4122.325 kb on - strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.347 kb on + strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.348 kb on - strand, within DZA65_RS19065 at 4122.379 kb on + strand, within DZA65_RS19065 at 4122.390 kb on - strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.399 kb on + strand, within DZA65_RS19065 at 4122.400 kb on - strand, within DZA65_RS19065 at 4122.400 kb on - strand, within DZA65_RS19065 at 4122.484 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.514 kb on + strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.515 kb on - strand at 4122.540 kb on + strand, within DZA65_RS19070 at 4122.541 kb on - strand, within DZA65_RS19070 at 4122.541 kb on - strand, within DZA65_RS19070 at 4122.550 kb on + strand, within DZA65_RS19070 at 4122.583 kb on + strand, within DZA65_RS19070 at 4122.583 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.676 kb on + strand, within DZA65_RS19070 at 4122.677 kb on - strand, within DZA65_RS19070 at 4122.677 kb on - strand, within DZA65_RS19070 at 4122.713 kb on + strand at 4122.714 kb on - strand at 4122.731 kb on + strand at 4122.732 kb on - strand at 4122.749 kb on + strand at 4122.766 kb on + strand at 4122.767 kb on - strand at 4122.767 kb on - strand at 4122.772 kb on + strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.773 kb on - strand at 4122.784 kb on + strand at 4122.784 kb on + strand at 4122.785 kb on - strand at 4122.785 kb on - strand at 4122.797 kb on + strand at 4122.797 kb on + strand at 4122.797 kb on + strand at 4122.798 kb on - strand at 4122.798 kb on - strand at 4122.798 kb on - strand at 4122.956 kb on - strand, within DZA65_RS19075 at 4122.956 kb on - strand, within DZA65_RS19075 at 4122.956 kb on - strand, within DZA65_RS19075 at 4122.956 kb on - strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.111 kb on + strand, within DZA65_RS19075 at 4123.112 kb on - strand at 4123.112 kb on - strand, within DZA65_RS19075 at 4123.112 kb on - strand, within DZA65_RS19075 at 4123.168 kb on + strand at 4123.184 kb on + strand at 4123.202 kb on + strand at 4123.243 kb on + strand at 4123.243 kb on + strand at 4123.251 kb on - strand at 4123.258 kb on + strand at 4123.273 kb on - strand at 4123.273 kb on - strand at 4123.484 kb on - strand at 4126.228 kb on - strand, within DZA65_RS19085 at 4126.391 kb on - strand at 4126.391 kb on - strand, within DZA65_RS19085 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.940 kb on + strand, within DZA65_RS19090 at 4126.958 kb on + strand, within DZA65_RS19090 at 4126.958 kb on + strand, within DZA65_RS19090 at 4126.959 kb on - strand, within DZA65_RS19090
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 4,122,316 + DZA65_RS19065 0.77 -1.4 4,122,316 + DZA65_RS19065 0.77 +0.6 4,122,317 - DZA65_RS19065 0.77 +0.0 4,122,317 - DZA65_RS19065 0.77 -1.8 4,122,324 + DZA65_RS19065 0.78 +2.4 4,122,325 - DZA65_RS19065 0.78 +2.5 4,122,325 - DZA65_RS19065 0.78 -0.5 4,122,347 + DZA65_RS19065 0.81 +1.1 4,122,347 + DZA65_RS19065 0.81 -1.0 4,122,347 + DZA65_RS19065 0.81 -1.5 4,122,347 + DZA65_RS19065 0.81 +0.0 4,122,347 + DZA65_RS19065 0.81 -0.6 4,122,347 + DZA65_RS19065 0.81 -1.3 4,122,347 + DZA65_RS19065 0.81 -0.5 4,122,347 + DZA65_RS19065 0.81 -0.5 4,122,348 - DZA65_RS19065 0.81 +0.1 4,122,348 - DZA65_RS19065 0.81 +2.7 4,122,348 - DZA65_RS19065 0.81 -1.3 4,122,348 - DZA65_RS19065 0.81 -2.0 4,122,348 - DZA65_RS19065 0.81 -1.4 4,122,379 + DZA65_RS19065 0.86 -0.5 4,122,390 - DZA65_RS19065 0.87 +1.2 4,122,399 + DZA65_RS19065 0.88 -0.6 4,122,399 + DZA65_RS19065 0.88 +1.8 4,122,399 + DZA65_RS19065 0.88 -0.4 4,122,399 + DZA65_RS19065 0.88 -1.5 4,122,400 - DZA65_RS19065 0.88 +1.0 4,122,400 - DZA65_RS19065 0.88 +1.3 4,122,484 + -1.3 4,122,514 + +0.3 4,122,514 + -0.8 4,122,514 + -1.8 4,122,514 + +1.8 4,122,514 + +0.9 4,122,515 - +1.4 4,122,515 - +0.9 4,122,515 - +2.6 4,122,515 - +2.0 4,122,515 - +0.5 4,122,515 - -1.0 4,122,540 + DZA65_RS19070 0.11 -0.3 4,122,541 - DZA65_RS19070 0.12 -0.5 4,122,541 - DZA65_RS19070 0.12 -0.5 4,122,550 + DZA65_RS19070 0.16 +0.7 4,122,583 + DZA65_RS19070 0.33 +0.8 4,122,583 + DZA65_RS19070 0.33 -1.1 4,122,676 + DZA65_RS19070 0.80 -1.4 4,122,676 + DZA65_RS19070 0.80 -1.3 4,122,676 + DZA65_RS19070 0.80 -1.0 4,122,676 + DZA65_RS19070 0.80 -1.8 4,122,676 + DZA65_RS19070 0.80 -1.5 4,122,676 + DZA65_RS19070 0.80 -0.3 4,122,676 + DZA65_RS19070 0.80 -0.3 4,122,677 - DZA65_RS19070 0.80 +0.3 4,122,677 - DZA65_RS19070 0.80 -1.8 4,122,713 + -1.3 4,122,714 - -0.3 4,122,731 + -1.5 4,122,732 - +0.4 4,122,749 + +0.5 4,122,766 + -0.0 4,122,767 - +0.7 4,122,767 - +0.8 4,122,772 + +1.0 4,122,773 - +0.7 4,122,773 - +0.3 4,122,773 - -1.0 4,122,773 - +0.0 4,122,773 - -1.3 4,122,784 + -2.3 4,122,784 + -0.3 4,122,785 - +2.0 4,122,785 - +2.5 4,122,797 + +1.9 4,122,797 + +0.0 4,122,797 + +0.7 4,122,798 - +0.0 4,122,798 - +1.0 4,122,798 - -0.5 4,122,956 - DZA65_RS19075 0.39 -2.0 4,122,956 - DZA65_RS19075 0.39 -2.0 4,122,956 - DZA65_RS19075 0.39 -0.4 4,122,956 - DZA65_RS19075 0.39 -0.3 4,123,111 + DZA65_RS19075 0.77 +0.0 4,123,111 + DZA65_RS19075 0.77 -1.8 4,123,111 + DZA65_RS19075 0.77 +0.5 4,123,111 + DZA65_RS19075 0.77 +0.5 4,123,112 - +1.0 4,123,112 - DZA65_RS19075 0.78 +0.0 4,123,112 - DZA65_RS19075 0.78 +0.8 4,123,168 + +0.5 4,123,184 + +0.6 4,123,202 + +0.8 4,123,243 + -1.5 4,123,243 + -1.4 4,123,251 - +0.6 4,123,258 + +1.0 4,123,273 - -2.4 4,123,273 - +0.5 4,123,484 - -1.5 4,126,228 - DZA65_RS19085 0.33 +0.0 4,126,391 - -1.0 4,126,391 - DZA65_RS19085 0.66 -0.5 4,126,940 + DZA65_RS19090 0.67 +0.5 4,126,940 + DZA65_RS19090 0.67 -1.1 4,126,940 + DZA65_RS19090 0.67 -0.2 4,126,958 + DZA65_RS19090 0.70 +2.0 4,126,958 + DZA65_RS19090 0.70 -1.3 4,126,959 - DZA65_RS19090 0.71 -0.4
Or see this region's nucleotide sequence