Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS02065 and DZA65_RS02070 are separated by 1 nucleotides DZA65_RS02070 and DZA65_RS02075 are separated by 221 nucleotides DZA65_RS02075 and DZA65_RS02080 are separated by 219 nucleotides
DZA65_RS02065: DZA65_RS02065 - LPS export ABC transporter permease LptF, at 456,145 to 457,263
_RS02065
DZA65_RS02070: DZA65_RS02070 - LPS export ABC transporter permease LptG, at 457,265 to 458,335
_RS02070
DZA65_RS02075: DZA65_RS02075 - tRNA-Leu, at 458,557 to 458,641
_RS02075
DZA65_RS02080: DZA65_RS02080 - tyrosine-type recombinase/integrase, at 458,861 to 460,129
_RS02080
Position (kb)
457
458
459 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 458.392 kb on - strand at 458.392 kb on - strand at 458.419 kb on + strand at 458.419 kb on + strand at 458.433 kb on - strand at 458.520 kb on + strand at 458.822 kb on - strand at 458.842 kb on + strand at 458.843 kb on - strand at 458.860 kb on + strand at 458.912 kb on + strand at 458.913 kb on - strand at 458.913 kb on - strand at 458.914 kb on + strand at 458.914 kb on + strand at 458.915 kb on - strand at 458.915 kb on - strand at 458.915 kb on - strand at 458.915 kb on - strand at 458.915 kb on - strand at 458.915 kb on - strand at 458.940 kb on - strand at 458.940 kb on - strand at 458.940 kb on - strand at 458.940 kb on - strand at 458.965 kb on + strand at 458.965 kb on + strand at 458.965 kb on + strand at 458.965 kb on + strand at 458.965 kb on + strand at 458.975 kb on + strand at 458.976 kb on - strand at 458.976 kb on - strand at 458.976 kb on - strand at 458.984 kb on + strand at 458.999 kb on - strand, within DZA65_RS02080 at 459.078 kb on + strand, within DZA65_RS02080 at 459.079 kb on - strand, within DZA65_RS02080 at 459.248 kb on - strand, within DZA65_RS02080 at 459.248 kb on - strand, within DZA65_RS02080 at 459.248 kb on - strand, within DZA65_RS02080 at 459.248 kb on - strand, within DZA65_RS02080 at 459.250 kb on + strand, within DZA65_RS02080 at 459.250 kb on + strand, within DZA65_RS02080 at 459.250 kb on + strand, within DZA65_RS02080 at 459.250 kb on + strand, within DZA65_RS02080 at 459.250 kb on + strand, within DZA65_RS02080 at 459.251 kb on - strand, within DZA65_RS02080 at 459.251 kb on - strand, within DZA65_RS02080 at 459.251 kb on - strand at 459.251 kb on - strand, within DZA65_RS02080 at 459.251 kb on - strand, within DZA65_RS02080
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 458,392 - +0.1 458,392 - -0.5 458,419 + -1.3 458,419 + +0.1 458,433 - -1.5 458,520 + -0.9 458,822 - -0.5 458,842 + -0.4 458,843 - +0.9 458,860 + +4.6 458,912 + +1.1 458,913 - -1.3 458,913 - -0.8 458,914 + -0.9 458,914 + -0.3 458,915 - -0.3 458,915 - +0.2 458,915 - +1.3 458,915 - -1.5 458,915 - -1.5 458,915 - -1.9 458,940 - +0.7 458,940 - +0.5 458,940 - -0.4 458,940 - -0.1 458,965 + -2.3 458,965 + +1.1 458,965 + +1.3 458,965 + -2.1 458,965 + +0.3 458,975 + +0.6 458,976 - -1.3 458,976 - +1.5 458,976 - +1.2 458,984 + +1.7 458,999 - DZA65_RS02080 0.11 +0.1 459,078 + DZA65_RS02080 0.17 -2.3 459,079 - DZA65_RS02080 0.17 +1.1 459,248 - DZA65_RS02080 0.30 -0.9 459,248 - DZA65_RS02080 0.30 +0.3 459,248 - DZA65_RS02080 0.30 +1.3 459,248 - DZA65_RS02080 0.30 -2.9 459,250 + DZA65_RS02080 0.31 -0.5 459,250 + DZA65_RS02080 0.31 +0.1 459,250 + DZA65_RS02080 0.31 -1.5 459,250 + DZA65_RS02080 0.31 +0.5 459,250 + DZA65_RS02080 0.31 -2.1 459,251 - DZA65_RS02080 0.31 -1.5 459,251 - DZA65_RS02080 0.31 +0.0 459,251 - -0.9 459,251 - DZA65_RS02080 0.31 -0.8 459,251 - DZA65_RS02080 0.31 -1.9
Or see this region's nucleotide sequence