Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01470 and DZA65_RS01475 are separated by 29 nucleotides DZA65_RS01475 and DZA65_RS01480 are separated by 190 nucleotides DZA65_RS01480 and DZA65_RS01485 are separated by 66 nucleotides DZA65_RS01485 and DZA65_RS01490 are separated by 179 nucleotides DZA65_RS01490 and DZA65_RS01495 overlap by 4 nucleotides
DZA65_RS01470: DZA65_RS01470 - LysE family transporter, at 325,919 to 326,545
_RS01470
DZA65_RS01475: DZA65_RS01475 - ribosome-associated protein, at 326,575 to 327,123
_RS01475
DZA65_RS01480: DZA65_RS01480 - metalloprotease PmbA, at 327,314 to 328,654
_RS01480
DZA65_RS01485: DZA65_RS01485 - nucleoside diphosphate kinase regulator, at 328,721 to 329,131
_RS01485
DZA65_RS01490: DZA65_RS01490 - PTS phosphocarrier protein NPr, at 329,311 to 329,583
_RS01490
DZA65_RS01495: DZA65_RS01495 - RNase adapter RapZ, at 329,580 to 330,434
_RS01495
Position (kb) 327
328
329 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 326.420 kb on + strand, within DZA65_RS01470 at 326.420 kb on + strand, within DZA65_RS01470 at 326.421 kb on - strand, within DZA65_RS01470 at 326.421 kb on - strand, within DZA65_RS01470 at 326.421 kb on - strand, within DZA65_RS01470 at 326.421 kb on - strand, within DZA65_RS01470 at 326.506 kb on + strand at 326.506 kb on + strand at 326.507 kb on - strand at 326.543 kb on + strand at 326.543 kb on + strand at 326.543 kb on + strand at 326.543 kb on + strand at 326.543 kb on + strand at 326.543 kb on + strand at 326.544 kb on - strand at 326.544 kb on - strand at 326.544 kb on - strand at 326.544 kb on - strand at 326.544 kb on - strand at 326.544 kb on - strand at 326.544 kb on - strand at 326.557 kb on + strand at 326.573 kb on + strand at 326.573 kb on + strand at 326.576 kb on + strand at 326.599 kb on + strand at 326.604 kb on - strand at 326.705 kb on + strand, within DZA65_RS01475 at 327.052 kb on + strand, within DZA65_RS01475 at 327.052 kb on + strand, within DZA65_RS01475 at 327.052 kb on + strand, within DZA65_RS01475 at 327.138 kb on - strand at 327.196 kb on + strand at 327.197 kb on - strand at 327.197 kb on - strand at 327.197 kb on - strand at 327.213 kb on + strand at 327.213 kb on + strand at 327.213 kb on + strand at 327.213 kb on + strand at 327.213 kb on + strand at 327.214 kb on - strand at 327.214 kb on - strand at 327.214 kb on - strand at 327.214 kb on - strand at 327.214 kb on - strand at 327.224 kb on - strand at 327.234 kb on + strand at 327.234 kb on + strand at 327.234 kb on + strand at 327.235 kb on - strand at 327.246 kb on + strand at 327.246 kb on + strand at 327.246 kb on + strand at 327.247 kb on - strand at 327.247 kb on - strand at 327.279 kb on + strand at 327.280 kb on - strand at 327.295 kb on + strand at 327.296 kb on - strand at 327.296 kb on - strand at 327.302 kb on + strand at 327.366 kb on + strand at 327.512 kb on + strand, within DZA65_RS01480 at 327.513 kb on - strand, within DZA65_RS01480 at 327.515 kb on + strand, within DZA65_RS01480 at 327.515 kb on + strand, within DZA65_RS01480 at 327.515 kb on + strand, within DZA65_RS01480 at 327.515 kb on + strand, within DZA65_RS01480 at 327.605 kb on + strand, within DZA65_RS01480 at 327.606 kb on - strand, within DZA65_RS01480 at 327.790 kb on + strand, within DZA65_RS01480 at 327.800 kb on + strand, within DZA65_RS01480 at 327.832 kb on + strand, within DZA65_RS01480 at 327.832 kb on + strand, within DZA65_RS01480 at 327.832 kb on + strand, within DZA65_RS01480 at 327.832 kb on + strand, within DZA65_RS01480 at 327.833 kb on - strand, within DZA65_RS01480 at 327.833 kb on - strand, within DZA65_RS01480 at 327.833 kb on - strand, within DZA65_RS01480 at 327.845 kb on + strand, within DZA65_RS01480 at 327.845 kb on + strand, within DZA65_RS01480 at 327.845 kb on + strand, within DZA65_RS01480 at 327.845 kb on + strand, within DZA65_RS01480 at 327.845 kb on + strand, within DZA65_RS01480 at 327.845 kb on + strand, within DZA65_RS01480 at 327.846 kb on - strand, within DZA65_RS01480 at 327.846 kb on - strand, within DZA65_RS01480 at 327.846 kb on - strand, within DZA65_RS01480 at 327.846 kb on - strand, within DZA65_RS01480 at 327.914 kb on + strand, within DZA65_RS01480 at 327.914 kb on + strand, within DZA65_RS01480 at 327.915 kb on - strand, within DZA65_RS01480 at 327.915 kb on - strand, within DZA65_RS01480 at 327.915 kb on - strand, within DZA65_RS01480 at 327.915 kb on - strand, within DZA65_RS01480 at 327.915 kb on - strand, within DZA65_RS01480 at 327.920 kb on + strand, within DZA65_RS01480 at 327.920 kb on + strand, within DZA65_RS01480 at 327.920 kb on + strand, within DZA65_RS01480 at 327.920 kb on + strand, within DZA65_RS01480 at 327.920 kb on + strand, within DZA65_RS01480 at 327.921 kb on - strand, within DZA65_RS01480 at 328.114 kb on + strand, within DZA65_RS01480 at 328.115 kb on - strand, within DZA65_RS01480 at 328.115 kb on - strand, within DZA65_RS01480 at 328.270 kb on - strand, within DZA65_RS01480 at 328.295 kb on + strand, within DZA65_RS01480 at 328.295 kb on + strand, within DZA65_RS01480 at 328.295 kb on + strand, within DZA65_RS01480 at 328.295 kb on + strand, within DZA65_RS01480 at 328.295 kb on + strand, within DZA65_RS01480 at 328.296 kb on - strand, within DZA65_RS01480 at 328.296 kb on - strand, within DZA65_RS01480 at 328.306 kb on + strand, within DZA65_RS01480 at 328.306 kb on + strand, within DZA65_RS01480 at 328.307 kb on - strand, within DZA65_RS01480 at 328.348 kb on + strand, within DZA65_RS01480 at 328.348 kb on + strand, within DZA65_RS01480 at 328.349 kb on - strand, within DZA65_RS01480 at 328.349 kb on - strand, within DZA65_RS01480 at 328.552 kb on + strand at 328.552 kb on + strand at 328.552 kb on + strand at 328.553 kb on - strand at 328.553 kb on - strand at 328.553 kb on - strand at 328.553 kb on - strand at 328.553 kb on - strand at 328.650 kb on + strand at 328.651 kb on - strand at 328.652 kb on + strand at 328.652 kb on + strand at 328.652 kb on + strand at 328.652 kb on + strand at 328.652 kb on + strand at 328.652 kb on + strand at 328.653 kb on - strand at 328.653 kb on - strand at 328.653 kb on - strand at 328.653 kb on - strand at 328.653 kb on - strand at 328.653 kb on - strand at 328.659 kb on + strand at 328.659 kb on + strand at 328.659 kb on + strand at 328.660 kb on - strand at 328.708 kb on + strand at 328.731 kb on + strand at 328.731 kb on + strand at 328.756 kb on - strand at 328.813 kb on + strand, within DZA65_RS01485 at 328.813 kb on + strand, within DZA65_RS01485 at 328.814 kb on - strand, within DZA65_RS01485 at 328.814 kb on - strand, within DZA65_RS01485 at 328.814 kb on - strand, within DZA65_RS01485 at 328.814 kb on - strand, within DZA65_RS01485 at 328.918 kb on + strand, within DZA65_RS01485 at 328.918 kb on + strand, within DZA65_RS01485 at 328.918 kb on + strand, within DZA65_RS01485 at 328.918 kb on + strand, within DZA65_RS01485 at 328.919 kb on - strand, within DZA65_RS01485 at 329.050 kb on + strand at 329.050 kb on + strand, within DZA65_RS01485 at 329.125 kb on + strand at 329.125 kb on + strand at 329.125 kb on + strand at 329.125 kb on + strand at 329.126 kb on - strand at 329.131 kb on + strand at 329.131 kb on + strand at 329.131 kb on + strand at 329.131 kb on + strand at 329.131 kb on + strand at 329.132 kb on - strand at 329.132 kb on - strand at 329.132 kb on - strand at 329.132 kb on - strand at 329.153 kb on + strand at 329.154 kb on - strand at 329.154 kb on - strand at 329.160 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.191 kb on + strand at 329.192 kb on - strand at 329.192 kb on - strand at 329.192 kb on - strand at 329.192 kb on - strand at 329.192 kb on - strand at 329.207 kb on + strand at 329.207 kb on + strand at 329.208 kb on - strand at 329.208 kb on - strand at 329.234 kb on + strand at 329.235 kb on - strand at 329.302 kb on + strand at 329.312 kb on + strand at 329.313 kb on - strand at 329.313 kb on - strand at 329.313 kb on - strand at 329.604 kb on - strand at 329.645 kb on - strand at 329.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 326,420 + DZA65_RS01470 0.80 +0.0 326,420 + DZA65_RS01470 0.80 +1.1 326,421 - DZA65_RS01470 0.80 +3.0 326,421 - DZA65_RS01470 0.80 +0.1 326,421 - DZA65_RS01470 0.80 -0.5 326,421 - DZA65_RS01470 0.80 +0.5 326,506 + -0.1 326,506 + -1.5 326,507 - -1.7 326,543 + +0.1 326,543 + +0.4 326,543 + +1.1 326,543 + +2.1 326,543 + -0.9 326,543 + +0.6 326,544 - -2.7 326,544 - -1.2 326,544 - -2.2 326,544 - -1.5 326,544 - +1.5 326,544 - +0.0 326,544 - -1.2 326,557 + +0.8 326,573 + +1.7 326,573 + +0.7 326,576 + -0.2 326,599 + -0.5 326,604 - +1.1 326,705 + DZA65_RS01475 0.24 -1.5 327,052 + DZA65_RS01475 0.87 -1.9 327,052 + DZA65_RS01475 0.87 -1.9 327,052 + DZA65_RS01475 0.87 +2.7 327,138 - +0.1 327,196 + -1.5 327,197 - -0.2 327,197 - -1.7 327,197 - -0.3 327,213 + +0.6 327,213 + +1.1 327,213 + -0.2 327,213 + +0.6 327,213 + +1.1 327,214 - +1.2 327,214 - +0.1 327,214 - +1.5 327,214 - -2.1 327,214 - +1.7 327,224 - -0.7 327,234 + -1.5 327,234 + +0.1 327,234 + -1.2 327,235 - -0.9 327,246 + -0.8 327,246 + +2.1 327,246 + +1.1 327,247 - +0.3 327,247 - +0.8 327,279 + -1.3 327,280 - +0.8 327,295 + +3.3 327,296 - +0.1 327,296 - +0.3 327,302 + +0.1 327,366 + +0.9 327,512 + DZA65_RS01480 0.15 -0.9 327,513 - DZA65_RS01480 0.15 -0.4 327,515 + DZA65_RS01480 0.15 +0.5 327,515 + DZA65_RS01480 0.15 -0.2 327,515 + DZA65_RS01480 0.15 +0.2 327,515 + DZA65_RS01480 0.15 +1.1 327,605 + DZA65_RS01480 0.22 -0.2 327,606 - DZA65_RS01480 0.22 +1.7 327,790 + DZA65_RS01480 0.35 -0.2 327,800 + DZA65_RS01480 0.36 -0.4 327,832 + DZA65_RS01480 0.39 -0.7 327,832 + DZA65_RS01480 0.39 +0.4 327,832 + DZA65_RS01480 0.39 -1.9 327,832 + DZA65_RS01480 0.39 -1.1 327,833 - DZA65_RS01480 0.39 -2.2 327,833 - DZA65_RS01480 0.39 +0.1 327,833 - DZA65_RS01480 0.39 -0.4 327,845 + DZA65_RS01480 0.40 -1.0 327,845 + DZA65_RS01480 0.40 -0.7 327,845 + DZA65_RS01480 0.40 -0.4 327,845 + DZA65_RS01480 0.40 +1.4 327,845 + DZA65_RS01480 0.40 +3.1 327,845 + DZA65_RS01480 0.40 +0.5 327,846 - DZA65_RS01480 0.40 +1.1 327,846 - DZA65_RS01480 0.40 +0.7 327,846 - DZA65_RS01480 0.40 +0.1 327,846 - DZA65_RS01480 0.40 -0.9 327,914 + DZA65_RS01480 0.45 -0.5 327,914 + DZA65_RS01480 0.45 +0.6 327,915 - DZA65_RS01480 0.45 -0.0 327,915 - DZA65_RS01480 0.45 -1.7 327,915 - DZA65_RS01480 0.45 +0.8 327,915 - DZA65_RS01480 0.45 -0.9 327,915 - DZA65_RS01480 0.45 -1.4 327,920 + DZA65_RS01480 0.45 -0.5 327,920 + DZA65_RS01480 0.45 -1.7 327,920 + DZA65_RS01480 0.45 +0.6 327,920 + DZA65_RS01480 0.45 +0.3 327,920 + DZA65_RS01480 0.45 +1.7 327,921 - DZA65_RS01480 0.45 -0.6 328,114 + DZA65_RS01480 0.60 -0.5 328,115 - DZA65_RS01480 0.60 +1.1 328,115 - DZA65_RS01480 0.60 +0.2 328,270 - DZA65_RS01480 0.71 +0.1 328,295 + DZA65_RS01480 0.73 -0.5 328,295 + DZA65_RS01480 0.73 -0.2 328,295 + DZA65_RS01480 0.73 +2.3 328,295 + DZA65_RS01480 0.73 +1.5 328,295 + DZA65_RS01480 0.73 +1.1 328,296 - DZA65_RS01480 0.73 -0.7 328,296 - DZA65_RS01480 0.73 -0.1 328,306 + DZA65_RS01480 0.74 +1.3 328,306 + DZA65_RS01480 0.74 +0.5 328,307 - DZA65_RS01480 0.74 -0.1 328,348 + DZA65_RS01480 0.77 -1.5 328,348 + DZA65_RS01480 0.77 -0.5 328,349 - DZA65_RS01480 0.77 -0.5 328,349 - DZA65_RS01480 0.77 +0.9 328,552 + +0.2 328,552 + -1.5 328,552 + -0.6 328,553 - -1.9 328,553 - -2.1 328,553 - +0.9 328,553 - +0.4 328,553 - +0.4 328,650 + -1.2 328,651 - +0.2 328,652 + -0.9 328,652 + -1.9 328,652 + +1.1 328,652 + +0.9 328,652 + -0.7 328,652 + +0.4 328,653 - +0.1 328,653 - +0.4 328,653 - +1.7 328,653 - -1.3 328,653 - -1.5 328,653 - -1.5 328,659 + -1.2 328,659 + -0.2 328,659 + -0.2 328,660 - -0.5 328,708 + +1.5 328,731 + -0.4 328,731 + -1.7 328,756 - +0.9 328,813 + DZA65_RS01485 0.22 +0.1 328,813 + DZA65_RS01485 0.22 +2.1 328,814 - DZA65_RS01485 0.23 -1.5 328,814 - DZA65_RS01485 0.23 -2.7 328,814 - DZA65_RS01485 0.23 -0.9 328,814 - DZA65_RS01485 0.23 +0.5 328,918 + DZA65_RS01485 0.48 -0.8 328,918 + DZA65_RS01485 0.48 -2.2 328,918 + DZA65_RS01485 0.48 +0.1 328,918 + DZA65_RS01485 0.48 +0.2 328,919 - DZA65_RS01485 0.48 -0.5 329,050 + -0.7 329,050 + DZA65_RS01485 0.80 -3.3 329,125 + +0.7 329,125 + +0.1 329,125 + -0.4 329,125 + -0.9 329,126 - +1.5 329,131 + -1.3 329,131 + -0.2 329,131 + -2.4 329,131 + +0.5 329,131 + -1.2 329,132 - -0.0 329,132 - -0.0 329,132 - -0.5 329,132 - -0.2 329,153 + -0.2 329,154 - +0.7 329,154 - +0.1 329,160 + -0.7 329,191 + +0.1 329,191 + -0.2 329,191 + +0.6 329,191 + +0.1 329,191 + -0.1 329,191 + +1.1 329,191 + -0.7 329,191 + +0.2 329,191 + -2.1 329,191 + -0.5 329,191 + -1.2 329,191 + -0.7 329,191 + +0.5 329,191 + +2.4 329,191 + -2.6 329,191 + -0.0 329,191 + -0.7 329,191 + -1.7 329,192 - -1.7 329,192 - -0.5 329,192 - -2.1 329,192 - +0.3 329,192 - -0.5 329,207 + -0.9 329,207 + -0.5 329,208 - +1.6 329,208 - -1.6 329,234 + -0.7 329,235 - +1.1 329,302 + -1.2 329,312 + +0.1 329,313 - -0.9 329,313 - +0.7 329,313 - +0.1 329,604 - -0.2 329,645 - +1.1 329,645 - -1.4
Or see this region's nucleotide sequence