Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS16905 and DZA65_RS16910 are separated by 16 nucleotides DZA65_RS16910 and DZA65_RS16915 are separated by 261 nucleotides DZA65_RS16915 and DZA65_RS16920 overlap by 4 nucleotides
DZA65_RS16905: DZA65_RS16905 - signal peptidase I, at 3,651,947 to 3,652,915
_RS16905
DZA65_RS16910: DZA65_RS16910 - elongation factor 4, at 3,652,932 to 3,654,731
_RS16910
DZA65_RS16915: DZA65_RS16915 - SoxR-reducing system protein RseC, at 3,654,993 to 3,655,463
_RS16915
DZA65_RS16920: DZA65_RS16920 - sigma-E factor regulatory protein RseB, at 3,655,460 to 3,656,410
_RS16920
Position (kb)
3652
3653
3654
3655 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3651.934 kb on + strand at 3651.935 kb on - strand at 3651.938 kb on + strand at 3651.939 kb on - strand at 3651.949 kb on - strand at 3651.949 kb on - strand at 3651.972 kb on - strand at 3652.934 kb on - strand at 3653.072 kb on - strand at 3653.124 kb on - strand, within DZA65_RS16910 at 3653.126 kb on + strand, within DZA65_RS16910 at 3653.127 kb on - strand, within DZA65_RS16910 at 3653.127 kb on - strand, within DZA65_RS16910 at 3653.127 kb on - strand, within DZA65_RS16910 at 3653.127 kb on - strand, within DZA65_RS16910 at 3653.225 kb on + strand, within DZA65_RS16910 at 3653.226 kb on - strand, within DZA65_RS16910 at 3653.226 kb on - strand, within DZA65_RS16910 at 3653.278 kb on - strand, within DZA65_RS16910 at 3653.278 kb on - strand, within DZA65_RS16910 at 3653.278 kb on - strand, within DZA65_RS16910 at 3653.315 kb on + strand, within DZA65_RS16910 at 3653.405 kb on - strand, within DZA65_RS16910 at 3653.405 kb on - strand, within DZA65_RS16910 at 3653.416 kb on - strand, within DZA65_RS16910 at 3653.431 kb on + strand, within DZA65_RS16910 at 3653.492 kb on - strand, within DZA65_RS16910 at 3653.584 kb on + strand, within DZA65_RS16910 at 3653.584 kb on + strand, within DZA65_RS16910 at 3653.585 kb on - strand, within DZA65_RS16910 at 3653.585 kb on - strand, within DZA65_RS16910 at 3653.585 kb on - strand, within DZA65_RS16910 at 3653.588 kb on + strand, within DZA65_RS16910 at 3653.589 kb on - strand, within DZA65_RS16910 at 3653.617 kb on + strand, within DZA65_RS16910 at 3653.618 kb on - strand, within DZA65_RS16910 at 3653.622 kb on + strand, within DZA65_RS16910 at 3653.623 kb on - strand, within DZA65_RS16910 at 3653.657 kb on - strand, within DZA65_RS16910 at 3653.657 kb on - strand, within DZA65_RS16910 at 3653.691 kb on - strand, within DZA65_RS16910 at 3653.813 kb on - strand, within DZA65_RS16910 at 3653.983 kb on - strand, within DZA65_RS16910 at 3654.038 kb on - strand, within DZA65_RS16910 at 3654.038 kb on - strand, within DZA65_RS16910 at 3654.038 kb on - strand, within DZA65_RS16910 at 3654.124 kb on + strand, within DZA65_RS16910 at 3654.124 kb on + strand, within DZA65_RS16910 at 3654.124 kb on + strand, within DZA65_RS16910 at 3654.284 kb on + strand, within DZA65_RS16910 at 3654.285 kb on - strand, within DZA65_RS16910 at 3654.382 kb on + strand, within DZA65_RS16910 at 3654.521 kb on - strand, within DZA65_RS16910 at 3654.544 kb on + strand, within DZA65_RS16910 at 3654.754 kb on + strand at 3654.755 kb on - strand at 3654.803 kb on - strand at 3654.816 kb on + strand at 3654.816 kb on + strand at 3654.836 kb on - strand at 3654.836 kb on - strand at 3654.836 kb on - strand at 3654.858 kb on + strand at 3654.859 kb on - strand at 3654.859 kb on - strand at 3654.859 kb on - strand at 3654.889 kb on + strand at 3654.937 kb on + strand at 3654.937 kb on + strand at 3654.938 kb on - strand at 3654.960 kb on + strand at 3654.960 kb on + strand at 3654.960 kb on + strand at 3654.961 kb on - strand at 3654.961 kb on - strand at 3654.961 kb on - strand at 3654.961 kb on - strand at 3654.961 kb on - strand at 3654.961 kb on - strand at 3654.962 kb on + strand at 3654.962 kb on + strand at 3654.963 kb on - strand at 3654.963 kb on - strand at 3655.073 kb on + strand, within DZA65_RS16915 at 3655.073 kb on + strand, within DZA65_RS16915 at 3655.102 kb on - strand, within DZA65_RS16915 at 3655.457 kb on + strand at 3655.457 kb on + strand at 3655.458 kb on - strand at 3655.573 kb on + strand, within DZA65_RS16920 at 3655.657 kb on - strand, within DZA65_RS16920 at 3655.657 kb on - strand, within DZA65_RS16920 at 3655.657 kb on - strand at 3655.721 kb on + strand, within DZA65_RS16920 at 3655.722 kb on - strand, within DZA65_RS16920
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 3,651,934 + -1.3 3,651,935 - -0.3 3,651,938 + +0.3 3,651,939 - -0.4 3,651,949 - -1.7 3,651,949 - -0.7 3,651,972 - -0.1 3,652,934 - -0.2 3,653,072 - +0.6 3,653,124 - DZA65_RS16910 0.11 +0.3 3,653,126 + DZA65_RS16910 0.11 -1.8 3,653,127 - DZA65_RS16910 0.11 -2.6 3,653,127 - DZA65_RS16910 0.11 -0.5 3,653,127 - DZA65_RS16910 0.11 -1.8 3,653,127 - DZA65_RS16910 0.11 -0.1 3,653,225 + DZA65_RS16910 0.16 -0.4 3,653,226 - DZA65_RS16910 0.16 -1.4 3,653,226 - DZA65_RS16910 0.16 -3.2 3,653,278 - DZA65_RS16910 0.19 -0.4 3,653,278 - DZA65_RS16910 0.19 -1.6 3,653,278 - DZA65_RS16910 0.19 -1.8 3,653,315 + DZA65_RS16910 0.21 -0.1 3,653,405 - DZA65_RS16910 0.26 +0.6 3,653,405 - DZA65_RS16910 0.26 -0.4 3,653,416 - DZA65_RS16910 0.27 +0.7 3,653,431 + DZA65_RS16910 0.28 +0.3 3,653,492 - DZA65_RS16910 0.31 -1.1 3,653,584 + DZA65_RS16910 0.36 -0.7 3,653,584 + DZA65_RS16910 0.36 -1.9 3,653,585 - DZA65_RS16910 0.36 -0.8 3,653,585 - DZA65_RS16910 0.36 -1.3 3,653,585 - DZA65_RS16910 0.36 +2.2 3,653,588 + DZA65_RS16910 0.36 -1.9 3,653,589 - DZA65_RS16910 0.36 -0.1 3,653,617 + DZA65_RS16910 0.38 -1.3 3,653,618 - DZA65_RS16910 0.38 -0.1 3,653,622 + DZA65_RS16910 0.38 -0.1 3,653,623 - DZA65_RS16910 0.38 -0.7 3,653,657 - DZA65_RS16910 0.40 -0.1 3,653,657 - DZA65_RS16910 0.40 -0.4 3,653,691 - DZA65_RS16910 0.42 -0.9 3,653,813 - DZA65_RS16910 0.49 +0.1 3,653,983 - DZA65_RS16910 0.58 -0.1 3,654,038 - DZA65_RS16910 0.61 +0.9 3,654,038 - DZA65_RS16910 0.61 +0.3 3,654,038 - DZA65_RS16910 0.61 -0.7 3,654,124 + DZA65_RS16910 0.66 -0.1 3,654,124 + DZA65_RS16910 0.66 +0.9 3,654,124 + DZA65_RS16910 0.66 -1.9 3,654,284 + DZA65_RS16910 0.75 +0.3 3,654,285 - DZA65_RS16910 0.75 -0.7 3,654,382 + DZA65_RS16910 0.81 -1.8 3,654,521 - DZA65_RS16910 0.88 -0.1 3,654,544 + DZA65_RS16910 0.90 +0.9 3,654,754 + -1.1 3,654,755 - +1.3 3,654,803 - -1.1 3,654,816 + -0.8 3,654,816 + +0.3 3,654,836 - +0.4 3,654,836 - -1.3 3,654,836 - -2.5 3,654,858 + -1.3 3,654,859 - -1.4 3,654,859 - +0.3 3,654,859 - -1.8 3,654,889 + -0.9 3,654,937 + -0.8 3,654,937 + -1.1 3,654,938 - -0.3 3,654,960 + +0.3 3,654,960 + +1.3 3,654,960 + -2.2 3,654,961 - -1.8 3,654,961 - +0.1 3,654,961 - +0.3 3,654,961 - -0.4 3,654,961 - -0.9 3,654,961 - +0.7 3,654,962 + -0.1 3,654,962 + -1.6 3,654,963 - +0.9 3,654,963 - -2.5 3,655,073 + DZA65_RS16915 0.17 -0.1 3,655,073 + DZA65_RS16915 0.17 +0.9 3,655,102 - DZA65_RS16915 0.23 -0.7 3,655,457 + -1.9 3,655,457 + -0.4 3,655,458 - -1.8 3,655,573 + DZA65_RS16920 0.12 +0.3 3,655,657 - DZA65_RS16920 0.21 -0.1 3,655,657 - DZA65_RS16920 0.21 -1.0 3,655,657 - -0.7 3,655,721 + DZA65_RS16920 0.27 -0.4 3,655,722 - DZA65_RS16920 0.28 -1.1
Or see this region's nucleotide sequence