Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS14965 and DZA65_RS14970 overlap by 4 nucleotides DZA65_RS14970 and DZA65_RS14975 are separated by 32 nucleotides DZA65_RS14975 and DZA65_RS14980 are separated by 117 nucleotides DZA65_RS14980 and DZA65_RS14985 are separated by 15 nucleotides
DZA65_RS14965: DZA65_RS14965 - NADH-quinone oxidoreductase subunit NuoF, at 3,265,715 to 3,267,064
_RS14965
DZA65_RS14970: DZA65_RS14970 - NADH-quinone oxidoreductase subunit NuoE, at 3,267,061 to 3,267,561
_RS14970
DZA65_RS14975: DZA65_RS14975 - NADH-quinone oxidoreductase subunit C/D, at 3,267,594 to 3,269,393
_RS14975
DZA65_RS14980: DZA65_RS14980 - NADH-quinone oxidoreductase subunit B, at 3,269,511 to 3,270,185
_RS14980
DZA65_RS14985: DZA65_RS14985 - NADH-quinone oxidoreductase subunit A, at 3,270,201 to 3,270,647
_RS14985
Position (kb) 3267
3268
3269
3270 Strain fitness (log2 ratio) -1
0
1 at 3269.175 kb on + strand, within DZA65_RS14975 at 3269.412 kb on - strand at 3269.412 kb on - strand at 3269.421 kb on - strand at 3269.480 kb on - strand at 3269.483 kb on + strand at 3269.538 kb on - strand at 3269.681 kb on - strand at 3270.176 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 3,269,175 + DZA65_RS14975 0.88 -0.0 3,269,412 - -0.4 3,269,412 - -0.2 3,269,421 - -1.5 3,269,480 - -1.0 3,269,483 + -0.2 3,269,538 - -0.9 3,269,681 - +0.2 3,270,176 + -1.2
Or see this region's nucleotide sequence