Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS11865 and DZA65_RS11870 are separated by 373 nucleotides DZA65_RS11870 and DZA65_RS11875 are separated by 171 nucleotides
DZA65_RS11865: DZA65_RS11865 - tRNA 2-thiouridine(34) synthase MnmA, at 2,532,561 to 2,533,673
_RS11865
DZA65_RS11870: DZA65_RS11870 - high frequency lysogenization protein HflD, at 2,534,047 to 2,534,679
_RS11870
DZA65_RS11875: DZA65_RS11875 - adenylosuccinate lyase, at 2,534,851 to 2,536,218
_RS11875
Position (kb)
2534
2535 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2533.201 kb on - strand, within DZA65_RS11865 at 2533.275 kb on + strand, within DZA65_RS11865 at 2533.344 kb on + strand, within DZA65_RS11865 at 2533.344 kb on + strand, within DZA65_RS11865 at 2533.381 kb on - strand, within DZA65_RS11865 at 2533.746 kb on + strand at 2533.746 kb on + strand at 2533.746 kb on + strand at 2533.746 kb on + strand at 2533.746 kb on + strand at 2533.746 kb on + strand at 2533.747 kb on - strand at 2533.747 kb on - strand at 2533.747 kb on - strand at 2533.747 kb on - strand at 2533.747 kb on - strand at 2533.747 kb on - strand at 2533.807 kb on + strand at 2533.807 kb on + strand at 2533.933 kb on + strand at 2533.933 kb on + strand at 2533.960 kb on - strand at 2533.960 kb on - strand at 2534.060 kb on - strand at 2534.070 kb on + strand at 2534.143 kb on - strand, within DZA65_RS11870 at 2534.154 kb on - strand, within DZA65_RS11870 at 2534.184 kb on + strand, within DZA65_RS11870 at 2534.184 kb on + strand, within DZA65_RS11870 at 2534.185 kb on - strand, within DZA65_RS11870 at 2534.227 kb on - strand, within DZA65_RS11870 at 2534.232 kb on - strand, within DZA65_RS11870 at 2534.259 kb on + strand, within DZA65_RS11870 at 2534.262 kb on - strand, within DZA65_RS11870 at 2534.262 kb on - strand, within DZA65_RS11870 at 2534.262 kb on - strand, within DZA65_RS11870 at 2534.306 kb on - strand, within DZA65_RS11870 at 2534.307 kb on + strand, within DZA65_RS11870 at 2534.307 kb on + strand, within DZA65_RS11870 at 2534.308 kb on - strand, within DZA65_RS11870 at 2534.308 kb on - strand, within DZA65_RS11870 at 2534.308 kb on - strand, within DZA65_RS11870 at 2534.308 kb on - strand, within DZA65_RS11870 at 2534.308 kb on - strand, within DZA65_RS11870 at 2534.410 kb on + strand, within DZA65_RS11870 at 2534.411 kb on - strand, within DZA65_RS11870 at 2534.437 kb on - strand, within DZA65_RS11870 at 2534.455 kb on + strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.456 kb on - strand, within DZA65_RS11870 at 2534.487 kb on + strand, within DZA65_RS11870 at 2534.718 kb on - strand at 2534.718 kb on - strand at 2534.744 kb on + strand at 2534.744 kb on + strand at 2534.744 kb on + strand at 2534.744 kb on + strand at 2534.744 kb on + strand at 2534.744 kb on + strand at 2534.744 kb on + strand at 2534.745 kb on - strand at 2534.745 kb on - strand at 2534.751 kb on + strand at 2534.751 kb on + strand at 2534.820 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 2,533,201 - DZA65_RS11865 0.58 -2.9 2,533,275 + DZA65_RS11865 0.64 -1.5 2,533,344 + DZA65_RS11865 0.70 -2.7 2,533,344 + DZA65_RS11865 0.70 -0.9 2,533,381 - DZA65_RS11865 0.74 -0.3 2,533,746 + -0.6 2,533,746 + -1.1 2,533,746 + -0.7 2,533,746 + -0.9 2,533,746 + -0.9 2,533,746 + -0.3 2,533,747 - -0.6 2,533,747 - -1.1 2,533,747 - -1.4 2,533,747 - -1.9 2,533,747 - +0.1 2,533,747 - -0.8 2,533,807 + -0.1 2,533,807 + -0.6 2,533,933 + -0.8 2,533,933 + -1.1 2,533,960 - -0.3 2,533,960 - -0.6 2,534,060 - -1.5 2,534,070 + -1.6 2,534,143 - DZA65_RS11870 0.15 -1.5 2,534,154 - DZA65_RS11870 0.17 +0.4 2,534,184 + DZA65_RS11870 0.22 +0.1 2,534,184 + DZA65_RS11870 0.22 -1.5 2,534,185 - DZA65_RS11870 0.22 -1.6 2,534,227 - DZA65_RS11870 0.28 -0.3 2,534,232 - DZA65_RS11870 0.29 -3.0 2,534,259 + DZA65_RS11870 0.33 -1.0 2,534,262 - DZA65_RS11870 0.34 -0.5 2,534,262 - DZA65_RS11870 0.34 -0.5 2,534,262 - DZA65_RS11870 0.34 -0.6 2,534,306 - DZA65_RS11870 0.41 -0.9 2,534,307 + DZA65_RS11870 0.41 +0.4 2,534,307 + DZA65_RS11870 0.41 -2.1 2,534,308 - DZA65_RS11870 0.41 -1.6 2,534,308 - DZA65_RS11870 0.41 +0.1 2,534,308 - DZA65_RS11870 0.41 +0.1 2,534,308 - DZA65_RS11870 0.41 -0.8 2,534,308 - DZA65_RS11870 0.41 -1.6 2,534,410 + DZA65_RS11870 0.57 -1.6 2,534,411 - DZA65_RS11870 0.58 -0.9 2,534,437 - DZA65_RS11870 0.62 -1.6 2,534,455 + DZA65_RS11870 0.64 -0.9 2,534,456 - DZA65_RS11870 0.65 -1.3 2,534,456 - DZA65_RS11870 0.65 -0.9 2,534,456 - DZA65_RS11870 0.65 -0.3 2,534,456 - DZA65_RS11870 0.65 -1.1 2,534,456 - DZA65_RS11870 0.65 -2.5 2,534,456 - DZA65_RS11870 0.65 +1.3 2,534,456 - DZA65_RS11870 0.65 -1.3 2,534,456 - DZA65_RS11870 0.65 -0.4 2,534,456 - DZA65_RS11870 0.65 +0.7 2,534,487 + DZA65_RS11870 0.70 +0.7 2,534,718 - -1.3 2,534,718 - -0.8 2,534,744 + +0.4 2,534,744 + -0.5 2,534,744 + -1.3 2,534,744 + -0.6 2,534,744 + -2.0 2,534,744 + -2.0 2,534,744 + +0.1 2,534,745 - -1.3 2,534,745 - -0.6 2,534,751 + -0.6 2,534,751 + +1.1 2,534,820 + -0.6
Or see this region's nucleotide sequence