Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS06810 and DZA65_RS06815 overlap by 11 nucleotides DZA65_RS06815 and DZA65_RS06820 are separated by 221 nucleotides DZA65_RS06820 and DZA65_RS06825 are separated by 655 nucleotides
DZA65_RS06810: DZA65_RS06810 - biotin-dependent carboxyltransferase family protein, at 1,413,747 to 1,414,676
_RS06810
DZA65_RS06815: DZA65_RS06815 - 5-oxoprolinase subunit PxpA, at 1,414,666 to 1,415,403
_RS06815
DZA65_RS06820: DZA65_RS06820 - citrate synthase, at 1,415,625 to 1,416,911
_RS06820
DZA65_RS06825: DZA65_RS06825 - succinate dehydrogenase cytochrome b556 subunit, at 1,417,567 to 1,417,956
_RS06825
Position (kb)
1415
1416
1417 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1414.638 kb on + strand at 1414.639 kb on - strand at 1414.670 kb on + strand at 1414.670 kb on + strand at 1414.671 kb on - strand at 1414.671 kb on - strand at 1414.671 kb on - strand at 1414.671 kb on - strand at 1414.671 kb on - strand at 1414.724 kb on + strand at 1414.724 kb on + strand at 1414.725 kb on - strand at 1414.725 kb on - strand at 1414.725 kb on - strand at 1414.725 kb on - strand at 1414.725 kb on - strand at 1414.900 kb on + strand, within DZA65_RS06815 at 1414.900 kb on + strand, within DZA65_RS06815 at 1414.901 kb on - strand, within DZA65_RS06815 at 1414.974 kb on + strand, within DZA65_RS06815 at 1414.975 kb on - strand at 1414.993 kb on + strand, within DZA65_RS06815 at 1414.993 kb on + strand, within DZA65_RS06815 at 1414.993 kb on + strand, within DZA65_RS06815 at 1414.993 kb on + strand, within DZA65_RS06815 at 1414.993 kb on + strand, within DZA65_RS06815 at 1414.993 kb on + strand, within DZA65_RS06815 at 1414.993 kb on + strand, within DZA65_RS06815 at 1414.994 kb on - strand, within DZA65_RS06815 at 1414.994 kb on - strand, within DZA65_RS06815 at 1414.994 kb on - strand, within DZA65_RS06815 at 1415.154 kb on + strand, within DZA65_RS06815 at 1415.154 kb on + strand, within DZA65_RS06815 at 1415.154 kb on + strand, within DZA65_RS06815 at 1415.154 kb on + strand, within DZA65_RS06815 at 1415.154 kb on + strand, within DZA65_RS06815 at 1415.155 kb on - strand, within DZA65_RS06815 at 1415.155 kb on - strand, within DZA65_RS06815 at 1415.155 kb on - strand, within DZA65_RS06815 at 1415.155 kb on - strand, within DZA65_RS06815 at 1415.155 kb on - strand, within DZA65_RS06815 at 1415.158 kb on + strand, within DZA65_RS06815 at 1415.158 kb on + strand, within DZA65_RS06815 at 1415.159 kb on - strand, within DZA65_RS06815 at 1415.384 kb on + strand at 1415.384 kb on + strand at 1415.384 kb on + strand at 1415.385 kb on - strand at 1415.495 kb on + strand at 1415.495 kb on + strand at 1415.495 kb on + strand at 1415.496 kb on - strand at 1415.496 kb on - strand at 1415.522 kb on + strand at 1415.522 kb on + strand at 1415.522 kb on + strand at 1415.523 kb on - strand at 1415.523 kb on - strand at 1415.523 kb on - strand at 1415.523 kb on - strand at 1415.523 kb on - strand at 1415.536 kb on - strand at 1415.536 kb on - strand at 1415.551 kb on - strand at 1415.557 kb on + strand at 1415.558 kb on - strand at 1415.558 kb on - strand at 1415.564 kb on + strand at 1415.984 kb on + strand, within DZA65_RS06820 at 1415.984 kb on + strand, within DZA65_RS06820 at 1415.986 kb on + strand, within DZA65_RS06820 at 1416.090 kb on - strand, within DZA65_RS06820 at 1416.648 kb on - strand, within DZA65_RS06820 at 1416.735 kb on - strand, within DZA65_RS06820 at 1416.736 kb on + strand, within DZA65_RS06820 at 1416.736 kb on + strand, within DZA65_RS06820 at 1416.813 kb on - strand at 1416.867 kb on - strand at 1416.932 kb on + strand at 1417.088 kb on + strand at 1417.089 kb on - strand at 1417.089 kb on - strand at 1417.089 kb on - strand at 1417.089 kb on - strand at 1417.093 kb on + strand at 1417.094 kb on - strand at 1417.137 kb on - strand at 1417.137 kb on - strand at 1417.144 kb on - strand at 1417.144 kb on - strand at 1417.144 kb on - strand at 1417.144 kb on - strand at 1417.148 kb on - strand at 1417.180 kb on + strand at 1417.180 kb on + strand at 1417.181 kb on - strand at 1417.181 kb on - strand at 1417.184 kb on + strand at 1417.184 kb on + strand at 1417.184 kb on + strand at 1417.184 kb on + strand at 1417.185 kb on - strand at 1417.185 kb on - strand at 1417.185 kb on - strand at 1417.208 kb on + strand at 1417.209 kb on - strand at 1417.209 kb on - strand at 1417.249 kb on - strand at 1417.255 kb on - strand at 1417.287 kb on - strand at 1417.287 kb on - strand at 1417.296 kb on + strand at 1417.301 kb on - strand at 1417.390 kb on + strand at 1417.424 kb on + strand at 1417.647 kb on + strand, within DZA65_RS06825 at 1417.647 kb on + strand, within DZA65_RS06825 at 1417.647 kb on + strand, within DZA65_RS06825 at 1417.648 kb on - strand, within DZA65_RS06825 at 1417.675 kb on - strand, within DZA65_RS06825 at 1417.675 kb on - strand, within DZA65_RS06825 at 1417.675 kb on - strand, within DZA65_RS06825 at 1417.675 kb on - strand, within DZA65_RS06825 at 1417.697 kb on + strand, within DZA65_RS06825 at 1417.697 kb on + strand, within DZA65_RS06825 at 1417.697 kb on + strand, within DZA65_RS06825 at 1417.697 kb on + strand, within DZA65_RS06825 at 1417.698 kb on - strand, within DZA65_RS06825 at 1417.698 kb on - strand, within DZA65_RS06825 at 1417.698 kb on - strand, within DZA65_RS06825 at 1417.818 kb on + strand, within DZA65_RS06825 at 1417.818 kb on + strand, within DZA65_RS06825 at 1417.818 kb on + strand, within DZA65_RS06825 at 1417.819 kb on - strand, within DZA65_RS06825 at 1417.819 kb on - strand, within DZA65_RS06825 at 1417.819 kb on - strand, within DZA65_RS06825 at 1417.819 kb on - strand, within DZA65_RS06825 at 1417.819 kb on - strand, within DZA65_RS06825 at 1417.823 kb on + strand, within DZA65_RS06825 at 1417.823 kb on + strand, within DZA65_RS06825 at 1417.823 kb on + strand, within DZA65_RS06825 at 1417.823 kb on + strand, within DZA65_RS06825 at 1417.824 kb on - strand, within DZA65_RS06825 at 1417.841 kb on + strand, within DZA65_RS06825 at 1417.841 kb on + strand, within DZA65_RS06825 at 1417.841 kb on + strand, within DZA65_RS06825 at 1417.841 kb on + strand, within DZA65_RS06825 at 1417.842 kb on - strand, within DZA65_RS06825 at 1417.842 kb on - strand, within DZA65_RS06825 at 1417.859 kb on - strand, within DZA65_RS06825
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 1,414,638 + -2.4 1,414,639 - -2.3 1,414,670 + +0.0 1,414,670 + +1.5 1,414,671 - -0.9 1,414,671 - -1.4 1,414,671 - -1.4 1,414,671 - -1.5 1,414,671 - -0.8 1,414,724 + -0.9 1,414,724 + +0.2 1,414,725 - -0.3 1,414,725 - +1.2 1,414,725 - -1.0 1,414,725 - -1.4 1,414,725 - +0.1 1,414,900 + DZA65_RS06815 0.32 +0.2 1,414,900 + DZA65_RS06815 0.32 +1.8 1,414,901 - DZA65_RS06815 0.32 -1.0 1,414,974 + DZA65_RS06815 0.42 +0.2 1,414,975 - +0.2 1,414,993 + DZA65_RS06815 0.44 +0.8 1,414,993 + DZA65_RS06815 0.44 -0.5 1,414,993 + DZA65_RS06815 0.44 +0.2 1,414,993 + DZA65_RS06815 0.44 +0.2 1,414,993 + DZA65_RS06815 0.44 +2.8 1,414,993 + DZA65_RS06815 0.44 +0.2 1,414,993 + DZA65_RS06815 0.44 -1.6 1,414,994 - DZA65_RS06815 0.44 -0.3 1,414,994 - DZA65_RS06815 0.44 -1.4 1,414,994 - DZA65_RS06815 0.44 +1.0 1,415,154 + DZA65_RS06815 0.66 -0.3 1,415,154 + DZA65_RS06815 0.66 -0.8 1,415,154 + DZA65_RS06815 0.66 +0.3 1,415,154 + DZA65_RS06815 0.66 -1.4 1,415,154 + DZA65_RS06815 0.66 +0.2 1,415,155 - DZA65_RS06815 0.66 -0.8 1,415,155 - DZA65_RS06815 0.66 -0.5 1,415,155 - DZA65_RS06815 0.66 -1.4 1,415,155 - DZA65_RS06815 0.66 -1.4 1,415,155 - DZA65_RS06815 0.66 -1.8 1,415,158 + DZA65_RS06815 0.67 +1.5 1,415,158 + DZA65_RS06815 0.67 -1.5 1,415,159 - DZA65_RS06815 0.67 -2.1 1,415,384 + -0.2 1,415,384 + -1.9 1,415,384 + -1.0 1,415,385 - -0.8 1,415,495 + -0.3 1,415,495 + +0.2 1,415,495 + -1.2 1,415,496 - +1.0 1,415,496 - -0.2 1,415,522 + -1.2 1,415,522 + -1.8 1,415,522 + -0.4 1,415,523 - -1.8 1,415,523 - +0.8 1,415,523 - -1.2 1,415,523 - -2.9 1,415,523 - -0.5 1,415,536 - -1.4 1,415,536 - -0.2 1,415,551 - -1.6 1,415,557 + -1.4 1,415,558 - -1.2 1,415,558 - -1.0 1,415,564 + -0.5 1,415,984 + DZA65_RS06820 0.28 -1.2 1,415,984 + DZA65_RS06820 0.28 -0.5 1,415,986 + DZA65_RS06820 0.28 -0.5 1,416,090 - DZA65_RS06820 0.36 +0.2 1,416,648 - DZA65_RS06820 0.79 -1.8 1,416,735 - DZA65_RS06820 0.86 -1.6 1,416,736 + DZA65_RS06820 0.86 -0.2 1,416,736 + DZA65_RS06820 0.86 +0.0 1,416,813 - -1.2 1,416,867 - -0.5 1,416,932 + +0.2 1,417,088 + -1.2 1,417,089 - -2.4 1,417,089 - +1.8 1,417,089 - -2.0 1,417,089 - -1.8 1,417,093 + +0.2 1,417,094 - -1.0 1,417,137 - +2.8 1,417,137 - -0.4 1,417,144 - -0.8 1,417,144 - +0.5 1,417,144 - +0.6 1,417,144 - +1.2 1,417,148 - -1.3 1,417,180 + -1.9 1,417,180 + -1.2 1,417,181 - -1.1 1,417,181 - -2.1 1,417,184 + -0.2 1,417,184 + -1.5 1,417,184 + -0.8 1,417,184 + -2.4 1,417,185 - +0.2 1,417,185 - +0.8 1,417,185 - +0.8 1,417,208 + -1.4 1,417,209 - -1.0 1,417,209 - +0.0 1,417,249 - +1.8 1,417,255 - +0.2 1,417,287 - -1.6 1,417,287 - +0.2 1,417,296 + -2.9 1,417,301 - -1.5 1,417,390 + -0.2 1,417,424 + -1.2 1,417,647 + DZA65_RS06825 0.21 -1.8 1,417,647 + DZA65_RS06825 0.21 +1.8 1,417,647 + DZA65_RS06825 0.21 -2.9 1,417,648 - DZA65_RS06825 0.21 -1.0 1,417,675 - DZA65_RS06825 0.28 -2.0 1,417,675 - DZA65_RS06825 0.28 +0.2 1,417,675 - DZA65_RS06825 0.28 -0.8 1,417,675 - DZA65_RS06825 0.28 -0.8 1,417,697 + DZA65_RS06825 0.33 -2.6 1,417,697 + DZA65_RS06825 0.33 -1.2 1,417,697 + DZA65_RS06825 0.33 -1.2 1,417,697 + DZA65_RS06825 0.33 +1.4 1,417,698 - DZA65_RS06825 0.34 -0.5 1,417,698 - DZA65_RS06825 0.34 -1.8 1,417,698 - DZA65_RS06825 0.34 -1.6 1,417,818 + DZA65_RS06825 0.64 -0.5 1,417,818 + DZA65_RS06825 0.64 -1.4 1,417,818 + DZA65_RS06825 0.64 -2.6 1,417,819 - DZA65_RS06825 0.65 -1.5 1,417,819 - DZA65_RS06825 0.65 -0.2 1,417,819 - DZA65_RS06825 0.65 -0.2 1,417,819 - DZA65_RS06825 0.65 -1.9 1,417,819 - DZA65_RS06825 0.65 -0.8 1,417,823 + DZA65_RS06825 0.66 -1.5 1,417,823 + DZA65_RS06825 0.66 -0.2 1,417,823 + DZA65_RS06825 0.66 -1.0 1,417,823 + DZA65_RS06825 0.66 -0.3 1,417,824 - DZA65_RS06825 0.66 -0.5 1,417,841 + DZA65_RS06825 0.70 +0.8 1,417,841 + DZA65_RS06825 0.70 +0.8 1,417,841 + DZA65_RS06825 0.70 -1.6 1,417,841 + DZA65_RS06825 0.70 -0.4 1,417,842 - DZA65_RS06825 0.71 -2.1 1,417,842 - DZA65_RS06825 0.71 -2.4 1,417,859 - DZA65_RS06825 0.75 +2.4
Or see this region's nucleotide sequence