Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS02870 and DZA65_RS02875 are separated by 21 nucleotides DZA65_RS02875 and DZA65_RS02880 are separated by 54 nucleotides DZA65_RS02880 and DZA65_RS02885 are separated by 129 nucleotides DZA65_RS02885 and DZA65_RS02890 are separated by 109 nucleotides
DZA65_RS02870: DZA65_RS02870 - chorismate lyase, at 616,602 to 617,117
_RS02870
DZA65_RS02875: DZA65_RS02875 - 4-hydroxybenzoate octaprenyltransferase, at 617,139 to 618,002
_RS02875
DZA65_RS02880: DZA65_RS02880 - glycerol-3-phosphate 1-O-acyltransferase PlsB, at 618,057 to 620,534
_RS02880
DZA65_RS02885: DZA65_RS02885 - diacylglycerol kinase, at 620,664 to 621,029
_RS02885
DZA65_RS02890: DZA65_RS02890 - repressor LexA, at 621,139 to 621,750
_RS02890
Position (kb) 618
619
620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 617.091 kb on + strand at 617.091 kb on + strand at 618.001 kb on - strand at 618.001 kb on - strand at 618.001 kb on - strand at 618.001 kb on - strand at 618.001 kb on - strand at 618.015 kb on + strand at 618.015 kb on + strand at 618.016 kb on - strand at 618.016 kb on - strand at 618.063 kb on + strand at 620.593 kb on + strand at 620.593 kb on + strand at 620.625 kb on - strand at 620.659 kb on + strand at 620.659 kb on + strand at 620.659 kb on + strand at 620.659 kb on + strand at 620.660 kb on - strand at 620.691 kb on - strand at 620.920 kb on - strand at 621.040 kb on - strand at 621.040 kb on - strand at 621.097 kb on + strand at 621.098 kb on - strand at 621.098 kb on - strand at 621.099 kb on + strand at 621.099 kb on + strand at 621.099 kb on + strand at 621.099 kb on + strand at 621.099 kb on + strand at 621.100 kb on - strand at 621.101 kb on + strand at 621.101 kb on + strand at 621.101 kb on + strand at 621.101 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 617,091 + -0.1 617,091 + -0.6 618,001 - +0.6 618,001 - -1.1 618,001 - -3.0 618,001 - +0.4 618,001 - -1.2 618,015 + -0.8 618,015 + -0.0 618,016 - -1.4 618,016 - +0.4 618,063 + -0.3 620,593 + +0.2 620,593 + -0.0 620,625 - -0.0 620,659 + -2.0 620,659 + -0.8 620,659 + -2.3 620,659 + -0.5 620,660 - +0.4 620,691 - -2.3 620,920 - -0.0 621,040 - -0.6 621,040 - -0.8 621,097 + -0.6 621,098 - +0.1 621,098 - -2.7 621,099 + -0.3 621,099 + -1.8 621,099 + +1.0 621,099 + -0.3 621,099 + -0.3 621,100 - -0.3 621,101 + -0.3 621,101 + -1.1 621,101 + -0.3 621,101 + -2.3
Or see this region's nucleotide sequence