Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS16235 and DZA65_RS16240 are separated by 853 nucleotides DZA65_RS16240 and DZA65_RS16245 are separated by 6 nucleotides DZA65_RS16245 and DZA65_RS16250 overlap by 23 nucleotides
DZA65_RS16235: DZA65_RS16235 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 3,522,320 to 3,523,138
_RS16235
DZA65_RS16240: DZA65_RS16240 - transporter substrate-binding domain-containing protein, at 3,523,992 to 3,524,750
_RS16240
DZA65_RS16245: DZA65_RS16245 - amino acid ABC transporter permease, at 3,524,757 to 3,525,458
_RS16245
DZA65_RS16250: DZA65_RS16250 - amino acid ABC transporter ATP-binding protein, at 3,525,436 to 3,526,161
_RS16250
Position (kb)
3523
3524
3525 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3523.024 kb on + strand, within DZA65_RS16235 at 3523.024 kb on + strand, within DZA65_RS16235 at 3523.041 kb on + strand, within DZA65_RS16235 at 3523.067 kb on - strand at 3523.067 kb on - strand at 3523.067 kb on - strand at 3523.138 kb on + strand at 3523.163 kb on + strand at 3523.163 kb on + strand at 3523.164 kb on - strand at 3523.226 kb on - strand at 3523.299 kb on - strand at 3523.309 kb on - strand at 3523.323 kb on - strand at 3523.323 kb on - strand at 3523.323 kb on - strand at 3523.326 kb on - strand at 3523.331 kb on + strand at 3523.429 kb on - strand at 3523.432 kb on + strand at 3523.445 kb on + strand at 3523.504 kb on + strand at 3523.510 kb on - strand at 3523.511 kb on + strand at 3523.514 kb on + strand at 3523.514 kb on + strand at 3523.515 kb on - strand at 3523.522 kb on + strand at 3523.552 kb on - strand at 3523.572 kb on + strand at 3523.573 kb on - strand at 3523.590 kb on - strand at 3523.655 kb on + strand at 3523.678 kb on - strand at 3523.820 kb on - strand at 3523.852 kb on + strand at 3523.948 kb on - strand at 3523.961 kb on + strand at 3523.965 kb on + strand at 3523.965 kb on + strand at 3523.966 kb on - strand at 3523.992 kb on - strand at 3523.992 kb on - strand at 3524.043 kb on - strand at 3524.117 kb on - strand, within DZA65_RS16240 at 3524.127 kb on - strand, within DZA65_RS16240 at 3524.172 kb on + strand, within DZA65_RS16240 at 3524.225 kb on + strand, within DZA65_RS16240 at 3524.225 kb on + strand, within DZA65_RS16240 at 3524.225 kb on + strand, within DZA65_RS16240 at 3524.294 kb on + strand, within DZA65_RS16240 at 3524.408 kb on - strand, within DZA65_RS16240 at 3524.497 kb on - strand, within DZA65_RS16240 at 3524.620 kb on + strand, within DZA65_RS16240 at 3524.631 kb on - strand, within DZA65_RS16240 at 3524.632 kb on + strand, within DZA65_RS16240 at 3524.632 kb on + strand, within DZA65_RS16240 at 3524.748 kb on + strand at 3524.756 kb on + strand at 3524.756 kb on + strand at 3524.757 kb on - strand at 3524.782 kb on + strand at 3524.787 kb on + strand at 3525.027 kb on - strand, within DZA65_RS16245 at 3525.028 kb on + strand, within DZA65_RS16245 at 3525.028 kb on + strand, within DZA65_RS16245 at 3525.028 kb on + strand, within DZA65_RS16245 at 3525.033 kb on - strand, within DZA65_RS16245 at 3525.108 kb on - strand, within DZA65_RS16245 at 3525.140 kb on - strand, within DZA65_RS16245 at 3525.243 kb on - strand, within DZA65_RS16245 at 3525.247 kb on + strand, within DZA65_RS16245 at 3525.435 kb on + strand at 3525.447 kb on - strand at 3525.707 kb on - strand, within DZA65_RS16250 at 3525.707 kb on - strand, within DZA65_RS16250
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 3,523,024 + DZA65_RS16235 0.86 -1.6 3,523,024 + DZA65_RS16235 0.86 -1.1 3,523,041 + DZA65_RS16235 0.88 -0.5 3,523,067 - +0.2 3,523,067 - -1.0 3,523,067 - +0.4 3,523,138 + -0.1 3,523,163 + -1.2 3,523,163 + -0.5 3,523,164 - -0.5 3,523,226 - +1.5 3,523,299 - -1.8 3,523,309 - -1.3 3,523,323 - -1.8 3,523,323 - -1.9 3,523,323 - -1.5 3,523,326 - -0.6 3,523,331 + -1.3 3,523,429 - -0.8 3,523,432 + -1.1 3,523,445 + -0.1 3,523,504 + +1.5 3,523,510 - -0.1 3,523,511 + -1.5 3,523,514 + -0.3 3,523,514 + -0.9 3,523,515 - +0.9 3,523,522 + -0.1 3,523,552 - -0.1 3,523,572 + -1.1 3,523,573 - -1.5 3,523,590 - -2.3 3,523,655 + -0.8 3,523,678 - -1.1 3,523,820 - +0.2 3,523,852 + -1.1 3,523,948 - +1.5 3,523,961 + +0.2 3,523,965 + -1.6 3,523,965 + -1.6 3,523,966 - +2.8 3,523,992 - -1.3 3,523,992 - -0.8 3,524,043 - -1.6 3,524,117 - DZA65_RS16240 0.16 -0.5 3,524,127 - DZA65_RS16240 0.18 -3.1 3,524,172 + DZA65_RS16240 0.24 -1.1 3,524,225 + DZA65_RS16240 0.31 -0.8 3,524,225 + DZA65_RS16240 0.31 -2.1 3,524,225 + DZA65_RS16240 0.31 +0.9 3,524,294 + DZA65_RS16240 0.40 -0.8 3,524,408 - DZA65_RS16240 0.55 +0.5 3,524,497 - DZA65_RS16240 0.67 -0.5 3,524,620 + DZA65_RS16240 0.83 -0.8 3,524,631 - DZA65_RS16240 0.84 +0.7 3,524,632 + DZA65_RS16240 0.84 -1.4 3,524,632 + DZA65_RS16240 0.84 +0.1 3,524,748 + -1.1 3,524,756 + -1.3 3,524,756 + -0.7 3,524,757 - +0.1 3,524,782 + -1.5 3,524,787 + -1.0 3,525,027 - DZA65_RS16245 0.38 -0.1 3,525,028 + DZA65_RS16245 0.39 -0.9 3,525,028 + DZA65_RS16245 0.39 -0.1 3,525,028 + DZA65_RS16245 0.39 -1.1 3,525,033 - DZA65_RS16245 0.39 +0.5 3,525,108 - DZA65_RS16245 0.50 -0.1 3,525,140 - DZA65_RS16245 0.55 -1.7 3,525,243 - DZA65_RS16245 0.69 -0.1 3,525,247 + DZA65_RS16245 0.70 -1.4 3,525,435 + -0.3 3,525,447 - -1.7 3,525,707 - DZA65_RS16250 0.37 -1.1 3,525,707 - DZA65_RS16250 0.37 +0.9
Or see this region's nucleotide sequence