Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS13160 and DZA65_RS13165 are separated by 244 nucleotides DZA65_RS13165 and DZA65_RS13170 are separated by 260 nucleotides DZA65_RS13170 and DZA65_RS13175 are separated by 254 nucleotides DZA65_RS13175 and DZA65_RS13180 are separated by 92 nucleotides
DZA65_RS13160: DZA65_RS13160 - tRNA-Val, at 2,821,879 to 2,821,955
_RS13160
DZA65_RS13165: DZA65_RS13165 - winged helix-turn-helix transcriptional regulator, at 2,822,200 to 2,822,661
_RS13165
DZA65_RS13170: DZA65_RS13170 - aldo/keto reductase, at 2,822,922 to 2,823,929
_RS13170
DZA65_RS13175: DZA65_RS13175 - LysR family transcriptional regulator, at 2,824,184 to 2,824,456
_RS13175
DZA65_RS13180: DZA65_RS13180 - alkene reductase, at 2,824,549 to 2,824,653
_RS13180
Position (kb)
2822
2823
2824 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2821.967 kb on - strand at 2821.979 kb on + strand at 2821.979 kb on + strand at 2821.980 kb on - strand at 2821.988 kb on + strand at 2822.039 kb on + strand at 2822.080 kb on + strand at 2822.081 kb on - strand at 2822.155 kb on + strand at 2822.156 kb on - strand at 2822.250 kb on + strand, within DZA65_RS13165 at 2822.250 kb on + strand, within DZA65_RS13165 at 2822.251 kb on - strand, within DZA65_RS13165 at 2822.251 kb on - strand, within DZA65_RS13165 at 2822.251 kb on - strand, within DZA65_RS13165 at 2822.528 kb on + strand, within DZA65_RS13165 at 2822.528 kb on + strand, within DZA65_RS13165 at 2822.528 kb on + strand, within DZA65_RS13165 at 2822.528 kb on + strand, within DZA65_RS13165 at 2822.528 kb on + strand, within DZA65_RS13165 at 2822.528 kb on + strand, within DZA65_RS13165 at 2822.528 kb on + strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.529 kb on - strand, within DZA65_RS13165 at 2822.543 kb on + strand, within DZA65_RS13165 at 2822.543 kb on + strand, within DZA65_RS13165 at 2822.543 kb on + strand, within DZA65_RS13165 at 2822.543 kb on + strand, within DZA65_RS13165 at 2822.544 kb on - strand, within DZA65_RS13165 at 2822.544 kb on - strand, within DZA65_RS13165 at 2822.576 kb on + strand, within DZA65_RS13165 at 2822.576 kb on + strand, within DZA65_RS13165 at 2822.576 kb on + strand, within DZA65_RS13165 at 2822.577 kb on - strand, within DZA65_RS13165 at 2822.577 kb on - strand, within DZA65_RS13165 at 2822.577 kb on - strand, within DZA65_RS13165 at 2822.577 kb on - strand, within DZA65_RS13165 at 2822.577 kb on - strand, within DZA65_RS13165 at 2822.585 kb on + strand, within DZA65_RS13165 at 2822.585 kb on + strand, within DZA65_RS13165 at 2822.631 kb on + strand at 2822.650 kb on - strand at 2822.650 kb on - strand at 2822.689 kb on - strand at 2822.725 kb on + strand at 2822.729 kb on + strand at 2822.730 kb on - strand at 2822.742 kb on + strand at 2822.743 kb on - strand at 2822.743 kb on - strand at 2822.743 kb on - strand at 2822.746 kb on + strand at 2822.746 kb on + strand at 2822.747 kb on - strand at 2822.759 kb on + strand at 2822.866 kb on + strand at 2822.866 kb on + strand at 2822.866 kb on + strand at 2822.866 kb on + strand at 2822.866 kb on + strand at 2822.867 kb on - strand at 2822.867 kb on - strand at 2822.867 kb on - strand at 2822.867 kb on - strand at 2822.868 kb on + strand at 2822.868 kb on + strand at 2822.868 kb on + strand at 2822.869 kb on - strand at 2822.869 kb on - strand at 2822.869 kb on - strand at 2822.887 kb on - strand at 2822.905 kb on + strand at 2822.928 kb on + strand at 2822.928 kb on + strand at 2822.928 kb on + strand at 2822.928 kb on + strand at 2822.928 kb on + strand at 2822.928 kb on + strand at 2822.929 kb on - strand at 2822.929 kb on - strand at 2822.929 kb on - strand at 2822.929 kb on - strand at 2822.929 kb on - strand at 2822.930 kb on + strand at 2822.930 kb on + strand at 2822.930 kb on + strand at 2822.930 kb on + strand at 2822.931 kb on - strand at 2822.931 kb on - strand at 2822.931 kb on - strand at 2822.931 kb on - strand at 2822.931 kb on - strand at 2822.931 kb on - strand at 2822.931 kb on - strand at 2823.007 kb on - strand at 2823.007 kb on - strand at 2823.048 kb on + strand, within DZA65_RS13170 at 2823.049 kb on - strand, within DZA65_RS13170 at 2823.090 kb on + strand, within DZA65_RS13170 at 2823.090 kb on + strand, within DZA65_RS13170 at 2823.090 kb on + strand, within DZA65_RS13170 at 2823.091 kb on - strand, within DZA65_RS13170 at 2823.091 kb on - strand, within DZA65_RS13170 at 2823.117 kb on - strand, within DZA65_RS13170 at 2823.227 kb on + strand, within DZA65_RS13170 at 2823.227 kb on + strand, within DZA65_RS13170 at 2823.227 kb on + strand, within DZA65_RS13170 at 2823.227 kb on + strand, within DZA65_RS13170 at 2823.228 kb on - strand, within DZA65_RS13170 at 2823.264 kb on - strand, within DZA65_RS13170 at 2823.285 kb on + strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.286 kb on - strand, within DZA65_RS13170 at 2823.373 kb on - strand, within DZA65_RS13170 at 2823.419 kb on + strand, within DZA65_RS13170 at 2823.420 kb on - strand, within DZA65_RS13170 at 2823.420 kb on - strand, within DZA65_RS13170 at 2823.446 kb on - strand, within DZA65_RS13170 at 2823.446 kb on - strand, within DZA65_RS13170 at 2823.449 kb on + strand, within DZA65_RS13170 at 2823.449 kb on + strand, within DZA65_RS13170 at 2823.450 kb on - strand, within DZA65_RS13170 at 2823.948 kb on + strand at 2823.948 kb on + strand at 2823.948 kb on + strand at 2823.948 kb on + strand at 2823.948 kb on + strand at 2823.948 kb on + strand at 2823.948 kb on + strand at 2823.949 kb on - strand at 2823.949 kb on - strand at 2823.949 kb on - strand at 2823.949 kb on - strand at 2823.949 kb on - strand at 2823.949 kb on - strand at 2823.949 kb on - strand at 2823.970 kb on + strand at 2823.970 kb on + strand at 2823.970 kb on + strand at 2824.043 kb on + strand at 2824.043 kb on + strand at 2824.043 kb on + strand at 2824.043 kb on + strand at 2824.044 kb on - strand at 2824.044 kb on - strand at 2824.044 kb on - strand at 2824.044 kb on - strand at 2824.044 kb on - strand at 2824.061 kb on + strand at 2824.134 kb on - strand at 2824.166 kb on + strand at 2824.166 kb on + strand at 2824.167 kb on - strand at 2824.167 kb on - strand at 2824.167 kb on - strand at 2824.167 kb on - strand at 2824.167 kb on - strand at 2824.170 kb on + strand at 2824.170 kb on + strand at 2824.171 kb on - strand at 2824.171 kb on - strand at 2824.171 kb on - strand at 2824.197 kb on + strand at 2824.197 kb on + strand at 2824.197 kb on + strand at 2824.197 kb on + strand at 2824.197 kb on + strand at 2824.197 kb on + strand at 2824.197 kb on + strand at 2824.198 kb on - strand at 2824.198 kb on - strand at 2824.198 kb on - strand at 2824.198 kb on - strand at 2824.198 kb on - strand at 2824.198 kb on - strand at 2824.201 kb on + strand at 2824.201 kb on + strand at 2824.201 kb on + strand at 2824.202 kb on - strand at 2824.212 kb on - strand, within DZA65_RS13175 at 2824.297 kb on + strand, within DZA65_RS13175 at 2824.297 kb on + strand, within DZA65_RS13175 at 2824.297 kb on + strand, within DZA65_RS13175 at 2824.297 kb on + strand, within DZA65_RS13175 at 2824.297 kb on + strand, within DZA65_RS13175 at 2824.298 kb on - strand, within DZA65_RS13175 at 2824.298 kb on - strand, within DZA65_RS13175 at 2824.312 kb on - strand, within DZA65_RS13175 at 2824.314 kb on + strand, within DZA65_RS13175 at 2824.314 kb on + strand, within DZA65_RS13175 at 2824.314 kb on + strand, within DZA65_RS13175 at 2824.314 kb on + strand, within DZA65_RS13175 at 2824.314 kb on + strand, within DZA65_RS13175 at 2824.314 kb on + strand, within DZA65_RS13175 at 2824.315 kb on - strand, within DZA65_RS13175 at 2824.315 kb on - strand, within DZA65_RS13175 at 2824.381 kb on + strand, within DZA65_RS13175 at 2824.460 kb on - strand at 2824.460 kb on - strand at 2824.475 kb on + strand at 2824.475 kb on + strand at 2824.475 kb on + strand at 2824.477 kb on + strand at 2824.477 kb on + strand at 2824.478 kb on - strand at 2824.509 kb on + strand at 2824.510 kb on - strand at 2824.563 kb on + strand, within DZA65_RS13180 at 2824.565 kb on + strand, within DZA65_RS13180 at 2824.566 kb on - strand, within DZA65_RS13180 at 2824.626 kb on + strand, within DZA65_RS13180 at 2824.626 kb on + strand, within DZA65_RS13180 at 2824.626 kb on + strand, within DZA65_RS13180 at 2824.659 kb on + strand at 2824.660 kb on - strand at 2824.660 kb on - strand at 2824.660 kb on - strand at 2824.660 kb on - strand at 2824.660 kb on - strand at 2824.660 kb on - strand at 2824.660 kb on - strand at 2824.663 kb on + strand at 2824.663 kb on + strand at 2824.701 kb on - strand at 2824.728 kb on + strand at 2824.740 kb on + strand at 2824.740 kb on + strand at 2824.740 kb on + strand at 2824.769 kb on - strand at 2824.796 kb on + strand at 2824.796 kb on + strand at 2824.796 kb on + strand at 2824.797 kb on - strand at 2824.797 kb on - strand at 2824.797 kb on - strand at 2824.797 kb on - strand at 2824.832 kb on - strand at 2824.851 kb on + strand at 2824.851 kb on + strand at 2824.871 kb on + strand at 2824.884 kb on + strand at 2824.884 kb on + strand at 2824.894 kb on + strand at 2824.895 kb on - strand at 2824.895 kb on - strand at 2824.895 kb on - strand at 2824.900 kb on - strand at 2824.910 kb on + strand at 2824.911 kb on - strand at 2824.914 kb on + strand at 2824.914 kb on + strand at 2824.914 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 2,821,967 - +0.1 2,821,979 + -0.8 2,821,979 + -0.8 2,821,980 - +0.2 2,821,988 + -1.9 2,822,039 + -1.3 2,822,080 + +1.1 2,822,081 - -0.9 2,822,155 + -1.1 2,822,156 - +0.5 2,822,250 + DZA65_RS13165 0.11 -1.1 2,822,250 + DZA65_RS13165 0.11 -0.4 2,822,251 - DZA65_RS13165 0.11 -0.2 2,822,251 - DZA65_RS13165 0.11 -0.1 2,822,251 - DZA65_RS13165 0.11 -0.9 2,822,528 + DZA65_RS13165 0.71 -0.5 2,822,528 + DZA65_RS13165 0.71 -1.6 2,822,528 + DZA65_RS13165 0.71 +4.0 2,822,528 + DZA65_RS13165 0.71 +0.2 2,822,528 + DZA65_RS13165 0.71 -1.2 2,822,528 + DZA65_RS13165 0.71 -0.2 2,822,528 + DZA65_RS13165 0.71 -1.0 2,822,529 - DZA65_RS13165 0.71 +0.1 2,822,529 - DZA65_RS13165 0.71 +0.6 2,822,529 - DZA65_RS13165 0.71 -0.2 2,822,529 - DZA65_RS13165 0.71 -0.6 2,822,529 - DZA65_RS13165 0.71 -2.2 2,822,529 - DZA65_RS13165 0.71 +1.2 2,822,529 - DZA65_RS13165 0.71 +0.1 2,822,529 - DZA65_RS13165 0.71 -0.9 2,822,529 - DZA65_RS13165 0.71 +0.3 2,822,529 - DZA65_RS13165 0.71 -1.1 2,822,543 + DZA65_RS13165 0.74 +0.2 2,822,543 + DZA65_RS13165 0.74 -0.1 2,822,543 + DZA65_RS13165 0.74 -0.4 2,822,543 + DZA65_RS13165 0.74 +1.5 2,822,544 - DZA65_RS13165 0.74 -0.2 2,822,544 - DZA65_RS13165 0.74 -1.6 2,822,576 + DZA65_RS13165 0.81 -2.1 2,822,576 + DZA65_RS13165 0.81 -0.2 2,822,576 + DZA65_RS13165 0.81 -0.4 2,822,577 - DZA65_RS13165 0.82 -1.1 2,822,577 - DZA65_RS13165 0.82 -0.2 2,822,577 - DZA65_RS13165 0.82 +0.7 2,822,577 - DZA65_RS13165 0.82 -2.2 2,822,577 - DZA65_RS13165 0.82 -2.6 2,822,585 + DZA65_RS13165 0.83 -0.8 2,822,585 + DZA65_RS13165 0.83 -1.1 2,822,631 + +1.1 2,822,650 - -0.6 2,822,650 - +0.1 2,822,689 - -0.9 2,822,725 + -1.0 2,822,729 + -2.5 2,822,730 - -1.1 2,822,742 + +0.2 2,822,743 - -1.1 2,822,743 - +2.6 2,822,743 - -1.6 2,822,746 + +1.6 2,822,746 + +0.5 2,822,747 - -0.8 2,822,759 + -2.2 2,822,866 + -0.7 2,822,866 + -0.5 2,822,866 + -1.1 2,822,866 + +0.5 2,822,866 + -0.2 2,822,867 - -0.8 2,822,867 - -0.8 2,822,867 - -0.8 2,822,867 - -0.6 2,822,868 + +0.0 2,822,868 + -0.8 2,822,868 + -1.6 2,822,869 - -0.6 2,822,869 - +0.3 2,822,869 - -0.9 2,822,887 - -1.1 2,822,905 + -0.6 2,822,928 + -0.1 2,822,928 + +0.2 2,822,928 + -0.6 2,822,928 + +0.2 2,822,928 + -1.4 2,822,928 + -0.4 2,822,929 - -0.1 2,822,929 - -0.6 2,822,929 - +0.2 2,822,929 - -1.0 2,822,929 - +1.0 2,822,930 + -0.7 2,822,930 + -1.4 2,822,930 + +0.2 2,822,930 + -0.9 2,822,931 - -0.8 2,822,931 - -0.4 2,822,931 - -1.4 2,822,931 - -0.4 2,822,931 - -1.4 2,822,931 - -0.1 2,822,931 - +0.8 2,823,007 - -1.1 2,823,007 - -0.6 2,823,048 + DZA65_RS13170 0.12 +1.2 2,823,049 - DZA65_RS13170 0.13 -0.4 2,823,090 + DZA65_RS13170 0.17 -0.9 2,823,090 + DZA65_RS13170 0.17 -0.1 2,823,090 + DZA65_RS13170 0.17 -1.2 2,823,091 - DZA65_RS13170 0.17 +1.2 2,823,091 - DZA65_RS13170 0.17 +2.8 2,823,117 - DZA65_RS13170 0.19 +0.2 2,823,227 + DZA65_RS13170 0.30 +1.2 2,823,227 + DZA65_RS13170 0.30 -2.2 2,823,227 + DZA65_RS13170 0.30 +0.0 2,823,227 + DZA65_RS13170 0.30 +0.2 2,823,228 - DZA65_RS13170 0.30 -0.4 2,823,264 - DZA65_RS13170 0.34 -0.2 2,823,285 + DZA65_RS13170 0.36 -0.4 2,823,286 - DZA65_RS13170 0.36 -1.1 2,823,286 - DZA65_RS13170 0.36 -0.1 2,823,286 - DZA65_RS13170 0.36 +0.2 2,823,286 - DZA65_RS13170 0.36 -0.9 2,823,286 - DZA65_RS13170 0.36 +0.4 2,823,286 - DZA65_RS13170 0.36 +0.5 2,823,286 - DZA65_RS13170 0.36 +0.2 2,823,286 - DZA65_RS13170 0.36 -1.6 2,823,286 - -1.1 2,823,286 - DZA65_RS13170 0.36 -0.4 2,823,286 - DZA65_RS13170 0.36 -0.2 2,823,373 - DZA65_RS13170 0.45 -1.6 2,823,419 + DZA65_RS13170 0.49 +0.4 2,823,420 - DZA65_RS13170 0.49 -1.8 2,823,420 - DZA65_RS13170 0.49 -1.2 2,823,446 - DZA65_RS13170 0.52 +0.5 2,823,446 - DZA65_RS13170 0.52 +0.2 2,823,449 + DZA65_RS13170 0.52 +0.1 2,823,449 + DZA65_RS13170 0.52 -0.1 2,823,450 - DZA65_RS13170 0.52 -0.5 2,823,948 + -0.8 2,823,948 + -1.5 2,823,948 + -1.4 2,823,948 + -1.8 2,823,948 + +1.2 2,823,948 + -2.8 2,823,948 + -1.2 2,823,949 - -0.8 2,823,949 - -1.8 2,823,949 - -0.5 2,823,949 - +0.2 2,823,949 - +0.6 2,823,949 - -1.6 2,823,949 - -0.1 2,823,970 + -1.8 2,823,970 + -1.1 2,823,970 + -2.7 2,824,043 + +2.7 2,824,043 + -0.8 2,824,043 + -1.0 2,824,043 + +0.6 2,824,044 - +0.2 2,824,044 - -0.4 2,824,044 - -1.4 2,824,044 - +0.9 2,824,044 - +0.4 2,824,061 + +0.4 2,824,134 - -0.4 2,824,166 + -0.3 2,824,166 + +0.6 2,824,167 - -0.4 2,824,167 - +0.2 2,824,167 - -0.8 2,824,167 - -1.6 2,824,167 - +0.2 2,824,170 + -1.1 2,824,170 + -0.6 2,824,171 - -1.1 2,824,171 - -1.1 2,824,171 - -0.8 2,824,197 + +0.8 2,824,197 + -1.8 2,824,197 + -0.4 2,824,197 + -0.9 2,824,197 + +0.9 2,824,197 + -0.2 2,824,197 + -1.1 2,824,198 - -0.5 2,824,198 - +2.2 2,824,198 - -1.9 2,824,198 - +0.5 2,824,198 - -1.8 2,824,198 - +0.9 2,824,201 + -1.6 2,824,201 + +2.3 2,824,201 + +2.2 2,824,202 - -0.7 2,824,212 - DZA65_RS13175 0.10 -0.0 2,824,297 + DZA65_RS13175 0.41 -0.4 2,824,297 + DZA65_RS13175 0.41 -0.9 2,824,297 + DZA65_RS13175 0.41 -0.4 2,824,297 + DZA65_RS13175 0.41 -0.4 2,824,297 + DZA65_RS13175 0.41 -1.8 2,824,298 - DZA65_RS13175 0.42 -1.4 2,824,298 - DZA65_RS13175 0.42 -0.6 2,824,312 - DZA65_RS13175 0.47 -0.9 2,824,314 + DZA65_RS13175 0.48 +0.0 2,824,314 + DZA65_RS13175 0.48 +4.3 2,824,314 + DZA65_RS13175 0.48 +0.2 2,824,314 + DZA65_RS13175 0.48 +2.6 2,824,314 + DZA65_RS13175 0.48 +0.5 2,824,314 + DZA65_RS13175 0.48 -2.1 2,824,315 - DZA65_RS13175 0.48 +0.1 2,824,315 - DZA65_RS13175 0.48 -0.8 2,824,381 + DZA65_RS13175 0.72 -1.4 2,824,460 - +0.0 2,824,460 - +0.2 2,824,475 + +0.2 2,824,475 + -0.8 2,824,475 + -1.6 2,824,477 + -1.1 2,824,477 + -2.1 2,824,478 - +1.2 2,824,509 + -1.1 2,824,510 - +0.4 2,824,563 + DZA65_RS13180 0.13 -2.9 2,824,565 + DZA65_RS13180 0.15 +0.0 2,824,566 - DZA65_RS13180 0.16 -0.9 2,824,626 + DZA65_RS13180 0.73 -0.5 2,824,626 + DZA65_RS13180 0.73 -0.6 2,824,626 + DZA65_RS13180 0.73 -1.3 2,824,659 + +1.2 2,824,660 - -3.6 2,824,660 - +0.5 2,824,660 - +0.1 2,824,660 - -1.1 2,824,660 - -0.2 2,824,660 - -0.1 2,824,660 - -1.2 2,824,663 + +0.5 2,824,663 + -0.6 2,824,701 - -0.5 2,824,728 + -2.7 2,824,740 + -1.0 2,824,740 + -0.6 2,824,740 + -0.3 2,824,769 - +0.8 2,824,796 + +1.0 2,824,796 + +0.2 2,824,796 + -1.1 2,824,797 - -1.6 2,824,797 - +0.2 2,824,797 - -1.7 2,824,797 - -2.0 2,824,832 - +0.7 2,824,851 + +0.2 2,824,851 + +0.8 2,824,871 + -1.8 2,824,884 + -0.0 2,824,884 + -1.9 2,824,894 + -0.8 2,824,895 - +0.4 2,824,895 - -0.3 2,824,895 - -0.1 2,824,900 - -0.8 2,824,910 + +0.7 2,824,911 - -1.3 2,824,914 + +3.9 2,824,914 + -0.8 2,824,914 + +1.2
Or see this region's nucleotide sequence