Experiment: Stem-Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS02090 and DZA65_RS02095 are separated by 131 nucleotides DZA65_RS02095 and DZA65_RS02100 are separated by 95 nucleotides DZA65_RS02100 and DZA65_RS02105 are separated by 222 nucleotides
DZA65_RS02090: DZA65_RS02090 - TIGR04141 family sporadically distributed protein, at 461,490 to 463,118
_RS02090
DZA65_RS02095: DZA65_RS02095 - helix-turn-helix transcriptional regulator, at 463,250 to 463,465
_RS02095
DZA65_RS02100: DZA65_RS02100 - transcriptional regulator, at 463,561 to 463,827
_RS02100
DZA65_RS02105: DZA65_RS02105 - AAA family ATPase, at 464,050 to 466,095
_RS02105
Position (kb) 463
464 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 462.621 kb on + strand, within DZA65_RS02090 at 462.678 kb on - strand, within DZA65_RS02090 at 462.679 kb on + strand, within DZA65_RS02090 at 463.000 kb on + strand at 463.025 kb on + strand at 463.026 kb on - strand at 463.252 kb on - strand at 463.401 kb on + strand, within DZA65_RS02095 at 463.402 kb on - strand at 463.402 kb on - strand, within DZA65_RS02095 at 463.402 kb on - strand, within DZA65_RS02095 at 463.402 kb on - strand, within DZA65_RS02095 at 463.488 kb on - strand at 463.488 kb on - strand at 463.488 kb on - strand at 463.491 kb on + strand at 463.491 kb on + strand at 463.491 kb on + strand at 463.492 kb on - strand at 463.492 kb on - strand at 463.501 kb on + strand at 463.501 kb on + strand at 463.502 kb on - strand at 463.502 kb on - strand at 463.516 kb on + strand at 463.519 kb on + strand at 463.519 kb on + strand at 463.520 kb on - strand at 463.520 kb on - strand at 463.523 kb on + strand at 463.523 kb on + strand at 463.523 kb on + strand at 463.523 kb on + strand at 463.523 kb on + strand at 463.523 kb on + strand at 463.524 kb on - strand at 463.524 kb on - strand at 463.524 kb on - strand at 463.524 kb on - strand at 463.524 kb on - strand at 463.524 kb on - strand at 463.542 kb on - strand at 463.542 kb on - strand at 463.542 kb on - strand at 463.542 kb on - strand at 463.544 kb on + strand at 463.548 kb on + strand at 463.549 kb on - strand at 463.549 kb on - strand at 463.549 kb on - strand at 463.560 kb on + strand at 463.560 kb on + strand at 463.560 kb on + strand at 463.560 kb on + strand at 463.561 kb on - strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.566 kb on + strand at 463.567 kb on - strand at 463.567 kb on - strand at 463.567 kb on - strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.571 kb on - strand at 463.571 kb on - strand at 463.571 kb on - strand at 463.571 kb on - strand at 463.685 kb on + strand, within DZA65_RS02100 at 463.686 kb on - strand, within DZA65_RS02100 at 463.686 kb on - strand, within DZA65_RS02100 at 463.762 kb on + strand, within DZA65_RS02100 at 463.825 kb on + strand at 463.825 kb on + strand at 463.825 kb on + strand at 463.825 kb on + strand at 463.825 kb on + strand at 463.826 kb on - strand at 463.826 kb on - strand at 463.826 kb on - strand at 463.826 kb on - strand at 463.826 kb on - strand at 463.841 kb on - strand at 463.841 kb on - strand at 463.869 kb on - strand at 463.890 kb on - strand at 463.968 kb on + strand at 463.969 kb on - strand at 463.973 kb on + strand at 463.973 kb on + strand at 463.973 kb on + strand at 463.973 kb on + strand at 463.973 kb on + strand at 463.973 kb on + strand at 463.973 kb on + strand at 463.973 kb on + strand at 463.974 kb on - strand at 463.974 kb on - strand at 463.974 kb on - strand at 463.974 kb on - strand at 463.974 kb on - strand at 463.975 kb on + strand at 463.975 kb on + strand at 463.975 kb on + strand at 463.975 kb on + strand at 463.975 kb on + strand at 463.976 kb on - strand at 463.976 kb on - strand at 463.977 kb on + strand at 463.977 kb on + strand at 463.977 kb on + strand at 463.977 kb on + strand at 463.977 kb on + strand at 463.977 kb on + strand at 463.978 kb on - strand at 463.978 kb on - strand at 464.052 kb on - strand at 464.052 kb on - strand at 464.052 kb on - strand at 464.052 kb on - strand at 464.131 kb on - strand at 464.182 kb on - strand at 464.381 kb on + strand, within DZA65_RS02105 at 464.405 kb on + strand, within DZA65_RS02105 at 464.406 kb on - strand, within DZA65_RS02105 at 464.490 kb on + strand, within DZA65_RS02105 at 464.491 kb on - strand, within DZA65_RS02105 at 464.568 kb on + strand, within DZA65_RS02105 at 464.569 kb on - strand, within DZA65_RS02105 at 464.569 kb on - strand, within DZA65_RS02105 at 464.569 kb on - strand, within DZA65_RS02105 at 464.569 kb on - strand, within DZA65_RS02105 at 464.569 kb on - strand, within DZA65_RS02105 at 464.603 kb on + strand, within DZA65_RS02105 at 464.604 kb on - strand, within DZA65_RS02105 at 464.604 kb on - strand, within DZA65_RS02105 at 464.620 kb on - strand, within DZA65_RS02105 at 464.680 kb on - strand, within DZA65_RS02105 at 464.680 kb on - strand, within DZA65_RS02105 at 464.680 kb on - strand, within DZA65_RS02105 at 464.699 kb on - strand, within DZA65_RS02105 at 464.699 kb on - strand, within DZA65_RS02105 at 464.722 kb on + strand, within DZA65_RS02105 at 464.722 kb on + strand, within DZA65_RS02105 at 464.723 kb on - strand, within DZA65_RS02105 at 464.723 kb on - strand, within DZA65_RS02105 at 464.723 kb on - strand, within DZA65_RS02105 at 464.723 kb on - strand, within DZA65_RS02105 at 464.776 kb on + strand, within DZA65_RS02105
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Upstate remove 462,621 + DZA65_RS02090 0.69 -2.1 462,678 - DZA65_RS02090 0.73 -0.6 462,679 + DZA65_RS02090 0.73 +1.8 463,000 + -0.1 463,025 + -1.8 463,026 - -1.4 463,252 - +0.8 463,401 + DZA65_RS02095 0.70 +0.4 463,402 - +0.2 463,402 - DZA65_RS02095 0.70 +0.2 463,402 - DZA65_RS02095 0.70 -2.4 463,402 - DZA65_RS02095 0.70 -0.2 463,488 - -0.4 463,488 - -1.4 463,488 - +0.2 463,491 + +1.3 463,491 + -1.1 463,491 + +1.2 463,492 - -0.2 463,492 - -1.1 463,501 + +0.5 463,501 + +1.3 463,502 - +2.2 463,502 - +0.5 463,516 + +0.2 463,519 + -1.2 463,519 + +0.3 463,520 - -1.8 463,520 - +0.3 463,523 + +0.4 463,523 + +1.9 463,523 + +0.4 463,523 + -1.7 463,523 + +0.9 463,523 + -2.7 463,524 - +1.0 463,524 - +2.8 463,524 - +0.2 463,524 - +1.3 463,524 - +0.8 463,524 - +1.2 463,542 - -0.8 463,542 - +1.2 463,542 - -0.8 463,542 - -0.6 463,544 + -0.8 463,548 + -1.7 463,549 - -1.5 463,549 - -1.7 463,549 - -0.6 463,560 + +0.8 463,560 + -0.2 463,560 + +0.1 463,560 + -0.8 463,561 - +0.3 463,566 + +0.3 463,566 + -1.8 463,566 + -1.4 463,566 + -0.8 463,566 + -0.1 463,566 + -0.4 463,566 + +1.8 463,566 + -0.2 463,566 + -2.6 463,566 + -0.7 463,567 - -1.1 463,567 - -0.6 463,567 - -1.1 463,570 + +1.2 463,570 + -0.2 463,570 + -0.6 463,570 + +0.5 463,570 + +0.4 463,570 + -0.7 463,570 + +0.2 463,570 + +1.8 463,570 + -2.1 463,571 - +0.4 463,571 - +1.3 463,571 - -2.5 463,571 - +0.2 463,685 + DZA65_RS02100 0.46 +1.2 463,686 - DZA65_RS02100 0.47 -0.6 463,686 - DZA65_RS02100 0.47 +1.0 463,762 + DZA65_RS02100 0.75 -1.9 463,825 + -1.4 463,825 + +0.1 463,825 + +1.3 463,825 + -1.2 463,825 + -0.8 463,826 - -1.1 463,826 - -1.6 463,826 - +0.2 463,826 - -1.6 463,826 - -1.2 463,841 - +1.5 463,841 - -0.4 463,869 - -1.1 463,890 - -0.8 463,968 + -0.7 463,969 - -1.1 463,973 + +1.2 463,973 + -0.4 463,973 + -2.2 463,973 + -0.6 463,973 + -0.6 463,973 + +0.1 463,973 + -0.1 463,973 + -0.2 463,974 - +0.2 463,974 - +1.3 463,974 - -0.7 463,974 - -1.6 463,974 - -1.1 463,975 + -0.2 463,975 + +0.2 463,975 + +0.9 463,975 + -1.3 463,975 + -1.6 463,976 - +1.3 463,976 - -0.9 463,977 + -1.2 463,977 + -0.8 463,977 + -1.9 463,977 + -0.2 463,977 + +3.5 463,977 + +0.4 463,978 - -0.8 463,978 - -1.9 464,052 - +0.2 464,052 - -1.6 464,052 - -2.3 464,052 - +0.2 464,131 - -0.7 464,182 - +1.2 464,381 + DZA65_RS02105 0.16 -0.7 464,405 + DZA65_RS02105 0.17 +0.4 464,406 - DZA65_RS02105 0.17 -1.4 464,490 + DZA65_RS02105 0.22 -1.8 464,491 - DZA65_RS02105 0.22 -0.2 464,568 + DZA65_RS02105 0.25 -0.2 464,569 - DZA65_RS02105 0.25 -0.6 464,569 - DZA65_RS02105 0.25 -0.4 464,569 - DZA65_RS02105 0.25 -0.8 464,569 - DZA65_RS02105 0.25 -0.4 464,569 - DZA65_RS02105 0.25 -0.3 464,603 + DZA65_RS02105 0.27 -0.8 464,604 - DZA65_RS02105 0.27 +0.5 464,604 - DZA65_RS02105 0.27 -0.2 464,620 - DZA65_RS02105 0.28 -0.3 464,680 - DZA65_RS02105 0.31 -0.4 464,680 - DZA65_RS02105 0.31 -0.6 464,680 - DZA65_RS02105 0.31 -1.1 464,699 - DZA65_RS02105 0.32 +0.6 464,699 - DZA65_RS02105 0.32 +1.8 464,722 + DZA65_RS02105 0.33 -0.2 464,722 + DZA65_RS02105 0.33 -1.1 464,723 - DZA65_RS02105 0.33 -0.4 464,723 - DZA65_RS02105 0.33 -0.6 464,723 - DZA65_RS02105 0.33 -2.1 464,723 - DZA65_RS02105 0.33 +1.2 464,776 + DZA65_RS02105 0.35 -1.7
Or see this region's nucleotide sequence