Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS11405 and DZA65_RS11410 are separated by 209 nucleotides DZA65_RS11410 and DZA65_RS11415 are separated by 3 nucleotides DZA65_RS11415 and DZA65_RS11420 are separated by 129 nucleotides
DZA65_RS11405: DZA65_RS11405 - glutamyl-tRNA reductase, at 2,426,569 to 2,427,825
_RS11405
DZA65_RS11410: DZA65_RS11410 - lipoprotein localization protein LolB, at 2,428,035 to 2,428,655
_RS11410
DZA65_RS11415: DZA65_RS11415 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 2,428,659 to 2,429,531
_RS11415
DZA65_RS11420: DZA65_RS11420 - ribose-phosphate diphosphokinase, at 2,429,661 to 2,430,608
_RS11420
Position (kb)
2428
2429
2430 Strain fitness (log2 ratio)
-2
-1
0
1 at 2427.850 kb on - strand at 2427.850 kb on - strand at 2427.853 kb on - strand at 2427.853 kb on - strand at 2427.878 kb on - strand at 2427.878 kb on - strand at 2428.017 kb on + strand at 2429.529 kb on + strand at 2429.529 kb on + strand at 2429.529 kb on + strand at 2429.557 kb on + strand at 2429.580 kb on + strand at 2429.580 kb on + strand at 2429.584 kb on + strand at 2429.584 kb on + strand at 2429.584 kb on + strand at 2429.584 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 2,427,850 - -1.4 2,427,850 - +0.9 2,427,853 - -1.9 2,427,853 - +0.2 2,427,878 - +0.0 2,427,878 - -1.4 2,428,017 + -1.1 2,429,529 + -1.3 2,429,529 + -1.4 2,429,529 + -1.8 2,429,557 + -0.8 2,429,580 + -0.8 2,429,580 + -1.6 2,429,584 + -0.1 2,429,584 + -2.6 2,429,584 + -1.9 2,429,584 + -0.7
Or see this region's nucleotide sequence