Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05425 and DZA65_RS05430 are separated by 18 nucleotides DZA65_RS05430 and DZA65_RS05435 are separated by 125 nucleotides DZA65_RS05435 and DZA65_RS05440 are separated by 143 nucleotides
DZA65_RS05425: DZA65_RS05425 - tRNA pseudouridine(65) synthase TruC, at 1,115,554 to 1,116,339
_RS05425
DZA65_RS05430: DZA65_RS05430 - flavodoxin, at 1,116,358 to 1,116,810
_RS05430
DZA65_RS05435: DZA65_RS05435 - DUF3461 family protein, at 1,116,936 to 1,117,322
_RS05435
DZA65_RS05440: DZA65_RS05440 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,117,466 to 1,118,290
_RS05440
Position (kb)
1116
1117
1118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1116.054 kb on + strand, within DZA65_RS05425 at 1116.054 kb on + strand, within DZA65_RS05425 at 1116.054 kb on + strand, within DZA65_RS05425 at 1116.054 kb on + strand, within DZA65_RS05425 at 1116.054 kb on + strand, within DZA65_RS05425 at 1116.054 kb on + strand at 1116.055 kb on - strand, within DZA65_RS05425 at 1116.055 kb on - strand, within DZA65_RS05425 at 1116.055 kb on - strand, within DZA65_RS05425 at 1116.055 kb on - strand, within DZA65_RS05425 at 1116.242 kb on + strand, within DZA65_RS05425 at 1116.242 kb on + strand, within DZA65_RS05425 at 1116.242 kb on + strand, within DZA65_RS05425 at 1116.243 kb on - strand, within DZA65_RS05425 at 1116.243 kb on - strand, within DZA65_RS05425 at 1116.243 kb on - strand, within DZA65_RS05425 at 1116.243 kb on - strand, within DZA65_RS05425 at 1116.243 kb on - strand, within DZA65_RS05425 at 1116.243 kb on - strand, within DZA65_RS05425 at 1116.243 kb on - strand, within DZA65_RS05425 at 1116.337 kb on + strand at 1116.337 kb on + strand at 1116.338 kb on - strand at 1116.338 kb on - strand at 1116.338 kb on - strand at 1116.338 kb on - strand at 1116.392 kb on - strand at 1116.392 kb on - strand at 1116.392 kb on - strand at 1116.455 kb on + strand, within DZA65_RS05430 at 1116.456 kb on - strand, within DZA65_RS05430 at 1116.480 kb on - strand, within DZA65_RS05430 at 1116.480 kb on - strand, within DZA65_RS05430 at 1116.496 kb on + strand, within DZA65_RS05430 at 1116.496 kb on + strand, within DZA65_RS05430 at 1116.496 kb on + strand, within DZA65_RS05430 at 1116.497 kb on - strand, within DZA65_RS05430 at 1116.558 kb on + strand, within DZA65_RS05430 at 1116.558 kb on + strand, within DZA65_RS05430 at 1116.559 kb on - strand, within DZA65_RS05430 at 1116.571 kb on + strand, within DZA65_RS05430 at 1116.571 kb on + strand, within DZA65_RS05430 at 1116.571 kb on + strand, within DZA65_RS05430 at 1116.572 kb on - strand, within DZA65_RS05430 at 1116.572 kb on - strand, within DZA65_RS05430 at 1116.572 kb on - strand, within DZA65_RS05430 at 1116.611 kb on + strand, within DZA65_RS05430 at 1116.611 kb on + strand, within DZA65_RS05430 at 1116.612 kb on - strand, within DZA65_RS05430 at 1116.612 kb on - strand, within DZA65_RS05430 at 1116.612 kb on - strand, within DZA65_RS05430 at 1116.646 kb on + strand, within DZA65_RS05430 at 1116.646 kb on + strand, within DZA65_RS05430 at 1116.647 kb on - strand, within DZA65_RS05430 at 1116.652 kb on - strand, within DZA65_RS05430 at 1116.804 kb on + strand at 1116.804 kb on + strand at 1116.805 kb on - strand at 1116.805 kb on - strand at 1116.805 kb on - strand at 1116.808 kb on + strand at 1116.808 kb on + strand at 1116.808 kb on + strand at 1116.808 kb on + strand at 1116.808 kb on + strand at 1116.808 kb on + strand at 1116.808 kb on + strand at 1116.809 kb on - strand at 1116.809 kb on - strand at 1116.809 kb on - strand at 1116.809 kb on - strand at 1116.809 kb on - strand at 1116.809 kb on - strand at 1116.809 kb on - strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.819 kb on + strand at 1116.820 kb on - strand at 1116.820 kb on - strand at 1116.820 kb on - strand at 1116.820 kb on - strand at 1116.820 kb on - strand at 1116.820 kb on - strand at 1116.820 kb on - strand at 1116.858 kb on + strand at 1116.858 kb on + strand at 1116.858 kb on + strand at 1116.858 kb on + strand at 1116.858 kb on + strand at 1116.859 kb on - strand at 1116.859 kb on - strand at 1116.859 kb on - strand at 1116.859 kb on - strand at 1116.865 kb on + strand at 1116.895 kb on - strand at 1116.895 kb on - strand at 1117.090 kb on + strand, within DZA65_RS05435 at 1117.091 kb on - strand, within DZA65_RS05435 at 1117.091 kb on - strand, within DZA65_RS05435 at 1117.091 kb on - strand, within DZA65_RS05435 at 1117.126 kb on + strand, within DZA65_RS05435 at 1117.126 kb on + strand, within DZA65_RS05435 at 1117.127 kb on - strand at 1117.127 kb on - strand, within DZA65_RS05435 at 1117.175 kb on - strand, within DZA65_RS05435 at 1117.193 kb on + strand, within DZA65_RS05435 at 1117.194 kb on - strand, within DZA65_RS05435 at 1117.238 kb on + strand, within DZA65_RS05435 at 1117.266 kb on - strand, within DZA65_RS05435 at 1117.266 kb on - strand, within DZA65_RS05435 at 1117.267 kb on + strand, within DZA65_RS05435 at 1117.267 kb on + strand, within DZA65_RS05435 at 1117.268 kb on - strand, within DZA65_RS05435 at 1117.277 kb on + strand, within DZA65_RS05435 at 1117.278 kb on - strand, within DZA65_RS05435 at 1117.318 kb on + strand at 1117.318 kb on + strand at 1117.318 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.322 kb on + strand at 1117.323 kb on - strand at 1117.323 kb on - strand at 1117.323 kb on - strand at 1117.323 kb on - strand at 1117.323 kb on - strand at 1117.342 kb on + strand at 1117.392 kb on - strand at 1117.431 kb on + strand at 1117.432 kb on - strand at 1117.432 kb on - strand at 1117.458 kb on + strand at 1117.464 kb on + strand at 1117.464 kb on + strand at 1117.464 kb on + strand at 1117.464 kb on + strand at 1117.465 kb on - strand at 1117.465 kb on - strand at 1117.467 kb on + strand at 1117.467 kb on + strand at 1117.467 kb on + strand at 1117.467 kb on + strand at 1117.467 kb on + strand at 1117.467 kb on + strand at 1117.468 kb on - strand at 1117.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 1,116,054 + DZA65_RS05425 0.64 +2.6 1,116,054 + DZA65_RS05425 0.64 -0.5 1,116,054 + DZA65_RS05425 0.64 -1.3 1,116,054 + DZA65_RS05425 0.64 -1.0 1,116,054 + DZA65_RS05425 0.64 -1.0 1,116,054 + -1.0 1,116,055 - DZA65_RS05425 0.64 +1.3 1,116,055 - DZA65_RS05425 0.64 -0.7 1,116,055 - DZA65_RS05425 0.64 -1.3 1,116,055 - DZA65_RS05425 0.64 -2.3 1,116,242 + DZA65_RS05425 0.88 +0.6 1,116,242 + DZA65_RS05425 0.88 +2.3 1,116,242 + DZA65_RS05425 0.88 -0.7 1,116,243 - DZA65_RS05425 0.88 +3.3 1,116,243 - DZA65_RS05425 0.88 -1.5 1,116,243 - DZA65_RS05425 0.88 -2.4 1,116,243 - DZA65_RS05425 0.88 +1.3 1,116,243 - DZA65_RS05425 0.88 +1.3 1,116,243 - DZA65_RS05425 0.88 +1.3 1,116,243 - DZA65_RS05425 0.88 +0.3 1,116,337 + -0.4 1,116,337 + +0.1 1,116,338 - +0.3 1,116,338 - -2.0 1,116,338 - -0.7 1,116,338 - +0.3 1,116,392 - -1.5 1,116,392 - -1.0 1,116,392 - +0.3 1,116,455 + DZA65_RS05430 0.21 +1.3 1,116,456 - DZA65_RS05430 0.22 +0.6 1,116,480 - DZA65_RS05430 0.27 -1.1 1,116,480 - DZA65_RS05430 0.27 -0.4 1,116,496 + DZA65_RS05430 0.30 -0.6 1,116,496 + DZA65_RS05430 0.30 -0.6 1,116,496 + DZA65_RS05430 0.30 +0.1 1,116,497 - DZA65_RS05430 0.31 -1.9 1,116,558 + DZA65_RS05430 0.44 +0.3 1,116,558 + DZA65_RS05430 0.44 -2.3 1,116,559 - DZA65_RS05430 0.44 -0.3 1,116,571 + DZA65_RS05430 0.47 +1.3 1,116,571 + DZA65_RS05430 0.47 +1.9 1,116,571 + DZA65_RS05430 0.47 -0.2 1,116,572 - DZA65_RS05430 0.47 +0.8 1,116,572 - DZA65_RS05430 0.47 -1.7 1,116,572 - DZA65_RS05430 0.47 -1.0 1,116,611 + DZA65_RS05430 0.56 -0.1 1,116,611 + DZA65_RS05430 0.56 +1.3 1,116,612 - DZA65_RS05430 0.56 -1.1 1,116,612 - DZA65_RS05430 0.56 +0.7 1,116,612 - DZA65_RS05430 0.56 +0.5 1,116,646 + DZA65_RS05430 0.64 +0.3 1,116,646 + DZA65_RS05430 0.64 +0.3 1,116,647 - DZA65_RS05430 0.64 -1.5 1,116,652 - DZA65_RS05430 0.65 -0.3 1,116,804 + -0.1 1,116,804 + -1.9 1,116,805 - -1.7 1,116,805 - +0.9 1,116,805 - +2.2 1,116,808 + -0.7 1,116,808 + +1.3 1,116,808 + +0.0 1,116,808 + -0.9 1,116,808 + +0.9 1,116,808 + -0.5 1,116,808 + +0.3 1,116,809 - +0.3 1,116,809 - -1.7 1,116,809 - +0.8 1,116,809 - -1.3 1,116,809 - -1.5 1,116,809 - +0.5 1,116,809 - +1.3 1,116,819 + -1.5 1,116,819 + -0.7 1,116,819 + -1.6 1,116,819 + +0.6 1,116,819 + +1.8 1,116,819 + -1.0 1,116,819 + +1.3 1,116,819 + -1.3 1,116,819 + -1.3 1,116,820 - +0.7 1,116,820 - -0.7 1,116,820 - +1.3 1,116,820 - +0.5 1,116,820 - -0.7 1,116,820 - -0.7 1,116,820 - +0.3 1,116,858 + -0.7 1,116,858 + -1.3 1,116,858 + -1.7 1,116,858 + +0.3 1,116,858 + -0.9 1,116,859 - +0.3 1,116,859 - -2.7 1,116,859 - -0.8 1,116,859 - -1.5 1,116,865 + +1.0 1,116,895 - +0.7 1,116,895 - -1.3 1,117,090 + DZA65_RS05435 0.40 +0.5 1,117,091 - DZA65_RS05435 0.40 +1.0 1,117,091 - DZA65_RS05435 0.40 -1.5 1,117,091 - DZA65_RS05435 0.40 -0.7 1,117,126 + DZA65_RS05435 0.49 -0.7 1,117,126 + DZA65_RS05435 0.49 -1.0 1,117,127 - -1.4 1,117,127 - DZA65_RS05435 0.49 -1.8 1,117,175 - DZA65_RS05435 0.62 +0.9 1,117,193 + DZA65_RS05435 0.66 +0.3 1,117,194 - DZA65_RS05435 0.67 +0.6 1,117,238 + DZA65_RS05435 0.78 -0.0 1,117,266 - DZA65_RS05435 0.85 +0.3 1,117,266 - DZA65_RS05435 0.85 -1.3 1,117,267 + DZA65_RS05435 0.86 -0.3 1,117,267 + DZA65_RS05435 0.86 -3.4 1,117,268 - DZA65_RS05435 0.86 +0.3 1,117,277 + DZA65_RS05435 0.88 +1.1 1,117,278 - DZA65_RS05435 0.88 +0.6 1,117,318 + +0.6 1,117,318 + -0.3 1,117,318 + -2.2 1,117,322 + -1.2 1,117,322 + -2.6 1,117,322 + +0.3 1,117,322 + -0.3 1,117,322 + +0.7 1,117,322 + -2.0 1,117,322 + +0.7 1,117,322 + +0.8 1,117,322 + -0.4 1,117,322 + +0.6 1,117,322 + +1.1 1,117,322 + -0.7 1,117,322 + -0.3 1,117,322 + +0.3 1,117,323 - -0.9 1,117,323 - -1.2 1,117,323 - +0.0 1,117,323 - -0.2 1,117,323 - -2.2 1,117,342 + +0.3 1,117,392 - -0.4 1,117,431 + +0.1 1,117,432 - -1.5 1,117,432 - +3.1 1,117,458 + -0.7 1,117,464 + -1.5 1,117,464 + +0.7 1,117,464 + +1.6 1,117,464 + +0.8 1,117,465 - -0.1 1,117,465 - -0.4 1,117,467 + +0.7 1,117,467 + -1.5 1,117,467 + -0.2 1,117,467 + +0.0 1,117,467 + -1.9 1,117,467 + +0.1 1,117,468 - -1.4 1,117,468 - -1.7
Or see this region's nucleotide sequence