Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS08120 and DZA65_RS08125 are separated by 10 nucleotides DZA65_RS08125 and DZA65_RS08130 are separated by 11 nucleotides DZA65_RS08130 and DZA65_RS08135 overlap by 4 nucleotides
DZA65_RS08120: DZA65_RS08120 - iron ABC transporter permease, at 1,700,539 to 1,701,561
_RS08120
DZA65_RS08125: DZA65_RS08125 - iron ABC transporter permease, at 1,701,572 to 1,702,603
_RS08125
DZA65_RS08130: DZA65_RS08130 - ABC transporter ATP-binding protein, at 1,702,615 to 1,703,418
_RS08130
DZA65_RS08135: DZA65_RS08135 - RraA family protein, at 1,703,415 to 1,704,032
_RS08135
Position (kb)
1701
1702
1703 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1701.006 kb on + strand, within DZA65_RS08120 at 1701.006 kb on + strand, within DZA65_RS08120 at 1701.007 kb on - strand, within DZA65_RS08120 at 1701.161 kb on - strand, within DZA65_RS08120 at 1701.212 kb on + strand, within DZA65_RS08120 at 1701.213 kb on - strand, within DZA65_RS08120 at 1701.213 kb on - strand, within DZA65_RS08120 at 1701.213 kb on - strand, within DZA65_RS08120 at 1701.213 kb on - strand, within DZA65_RS08120 at 1701.213 kb on - strand, within DZA65_RS08120 at 1701.233 kb on + strand, within DZA65_RS08120 at 1701.360 kb on + strand, within DZA65_RS08120 at 1701.559 kb on + strand at 1701.559 kb on + strand at 1701.781 kb on - strand, within DZA65_RS08125 at 1701.926 kb on + strand, within DZA65_RS08125 at 1701.927 kb on - strand, within DZA65_RS08125 at 1701.927 kb on - strand, within DZA65_RS08125 at 1701.962 kb on + strand, within DZA65_RS08125 at 1701.962 kb on + strand, within DZA65_RS08125 at 1701.962 kb on + strand, within DZA65_RS08125 at 1701.962 kb on + strand, within DZA65_RS08125 at 1701.962 kb on + strand, within DZA65_RS08125 at 1701.963 kb on - strand, within DZA65_RS08125 at 1701.963 kb on - strand, within DZA65_RS08125 at 1701.963 kb on - strand, within DZA65_RS08125 at 1702.013 kb on + strand, within DZA65_RS08125 at 1702.013 kb on + strand, within DZA65_RS08125 at 1702.014 kb on - strand, within DZA65_RS08125 at 1702.084 kb on - strand, within DZA65_RS08125 at 1702.146 kb on - strand, within DZA65_RS08125 at 1702.146 kb on - strand, within DZA65_RS08125 at 1702.146 kb on - strand, within DZA65_RS08125 at 1702.169 kb on + strand, within DZA65_RS08125 at 1702.169 kb on + strand, within DZA65_RS08125 at 1702.261 kb on - strand, within DZA65_RS08125 at 1702.397 kb on - strand, within DZA65_RS08125 at 1702.577 kb on + strand at 1702.581 kb on + strand at 1702.581 kb on + strand at 1702.581 kb on + strand at 1702.582 kb on - strand at 1702.582 kb on - strand at 1702.582 kb on - strand at 1702.582 kb on - strand at 1702.744 kb on - strand, within DZA65_RS08130 at 1702.744 kb on - strand, within DZA65_RS08130 at 1702.744 kb on - strand, within DZA65_RS08130 at 1702.769 kb on + strand, within DZA65_RS08130 at 1702.812 kb on + strand, within DZA65_RS08130 at 1702.890 kb on + strand, within DZA65_RS08130 at 1702.891 kb on - strand, within DZA65_RS08130 at 1702.941 kb on - strand, within DZA65_RS08130 at 1702.941 kb on - strand, within DZA65_RS08130 at 1703.115 kb on + strand, within DZA65_RS08130 at 1703.116 kb on - strand, within DZA65_RS08130 at 1703.116 kb on - strand, within DZA65_RS08130 at 1703.116 kb on - strand, within DZA65_RS08130 at 1703.196 kb on + strand, within DZA65_RS08130 at 1703.196 kb on + strand, within DZA65_RS08130 at 1703.196 kb on + strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.197 kb on - strand, within DZA65_RS08130 at 1703.313 kb on + strand, within DZA65_RS08130 at 1703.313 kb on + strand, within DZA65_RS08130 at 1703.325 kb on + strand, within DZA65_RS08130 at 1703.326 kb on - strand, within DZA65_RS08130 at 1703.376 kb on + strand at 1703.376 kb on + strand at 1703.377 kb on - strand at 1703.520 kb on + strand, within DZA65_RS08135 at 1703.521 kb on - strand, within DZA65_RS08135
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 1,701,006 + DZA65_RS08120 0.46 -2.8 1,701,006 + DZA65_RS08120 0.46 -2.1 1,701,007 - DZA65_RS08120 0.46 -3.3 1,701,161 - DZA65_RS08120 0.61 -1.1 1,701,212 + DZA65_RS08120 0.66 -1.8 1,701,213 - DZA65_RS08120 0.66 -1.2 1,701,213 - DZA65_RS08120 0.66 -1.2 1,701,213 - DZA65_RS08120 0.66 -1.8 1,701,213 - DZA65_RS08120 0.66 +0.2 1,701,213 - DZA65_RS08120 0.66 -3.7 1,701,233 + DZA65_RS08120 0.68 -2.0 1,701,360 + DZA65_RS08120 0.80 -2.5 1,701,559 + -1.1 1,701,559 + -2.5 1,701,781 - DZA65_RS08125 0.20 -1.5 1,701,926 + DZA65_RS08125 0.34 -1.8 1,701,927 - DZA65_RS08125 0.34 -0.2 1,701,927 - DZA65_RS08125 0.34 -1.8 1,701,962 + DZA65_RS08125 0.38 -2.0 1,701,962 + DZA65_RS08125 0.38 -3.1 1,701,962 + DZA65_RS08125 0.38 +0.8 1,701,962 + DZA65_RS08125 0.38 -1.8 1,701,962 + DZA65_RS08125 0.38 -1.2 1,701,963 - DZA65_RS08125 0.38 -0.4 1,701,963 - DZA65_RS08125 0.38 -1.2 1,701,963 - DZA65_RS08125 0.38 -3.3 1,702,013 + DZA65_RS08125 0.43 -1.5 1,702,013 + DZA65_RS08125 0.43 -0.4 1,702,014 - DZA65_RS08125 0.43 -2.2 1,702,084 - DZA65_RS08125 0.50 -1.6 1,702,146 - DZA65_RS08125 0.56 -0.5 1,702,146 - DZA65_RS08125 0.56 -0.9 1,702,146 - DZA65_RS08125 0.56 -0.9 1,702,169 + DZA65_RS08125 0.58 +0.1 1,702,169 + DZA65_RS08125 0.58 +1.4 1,702,261 - DZA65_RS08125 0.67 -1.7 1,702,397 - DZA65_RS08125 0.80 -1.8 1,702,577 + -1.5 1,702,581 + -0.0 1,702,581 + -1.5 1,702,581 + -0.0 1,702,582 - -0.4 1,702,582 - -1.5 1,702,582 - -2.4 1,702,582 - -1.8 1,702,744 - DZA65_RS08130 0.16 -1.2 1,702,744 - DZA65_RS08130 0.16 -0.8 1,702,744 - DZA65_RS08130 0.16 -1.5 1,702,769 + DZA65_RS08130 0.19 -3.3 1,702,812 + DZA65_RS08130 0.25 -1.5 1,702,890 + DZA65_RS08130 0.34 -2.4 1,702,891 - DZA65_RS08130 0.34 -1.2 1,702,941 - DZA65_RS08130 0.41 -0.8 1,702,941 - DZA65_RS08130 0.41 -2.4 1,703,115 + DZA65_RS08130 0.62 -1.2 1,703,116 - DZA65_RS08130 0.62 +2.3 1,703,116 - DZA65_RS08130 0.62 -2.4 1,703,116 - DZA65_RS08130 0.62 -1.9 1,703,196 + DZA65_RS08130 0.72 -2.0 1,703,196 + DZA65_RS08130 0.72 -1.5 1,703,196 + DZA65_RS08130 0.72 -4.2 1,703,197 - DZA65_RS08130 0.72 -0.5 1,703,197 - DZA65_RS08130 0.72 -3.2 1,703,197 - DZA65_RS08130 0.72 -1.8 1,703,197 - DZA65_RS08130 0.72 -2.1 1,703,197 - DZA65_RS08130 0.72 -1.6 1,703,197 - DZA65_RS08130 0.72 -1.8 1,703,197 - DZA65_RS08130 0.72 -3.3 1,703,197 - DZA65_RS08130 0.72 -2.7 1,703,313 + DZA65_RS08130 0.87 +0.3 1,703,313 + DZA65_RS08130 0.87 -5.0 1,703,325 + DZA65_RS08130 0.88 -3.5 1,703,326 - DZA65_RS08130 0.88 +0.3 1,703,376 + +0.2 1,703,376 + +0.1 1,703,377 - -1.2 1,703,520 + DZA65_RS08135 0.17 -1.8 1,703,521 - DZA65_RS08135 0.17 -0.6
Or see this region's nucleotide sequence