Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01245 and DZA65_RS01250 are separated by 4 nucleotides DZA65_RS01250 and DZA65_RS01255 are separated by 82 nucleotides DZA65_RS01255 and DZA65_RS01260 are separated by 15 nucleotides
DZA65_RS01245: DZA65_RS01245 - DNA-binding protein HU-alpha, at 277,495 to 277,767
_RS01245
DZA65_RS01250: DZA65_RS01250 - DUF1481 domain-containing protein, at 277,772 to 278,500
_RS01250
DZA65_RS01255: DZA65_RS01255 - phosphoribosylamine--glycine ligase, at 278,583 to 279,872
_RS01255
DZA65_RS01260: DZA65_RS01260 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 279,888 to 281,477
_RS01260
Position (kb) 278
279
280 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 277.879 kb on + strand, within DZA65_RS01250 at 277.879 kb on + strand, within DZA65_RS01250 at 277.879 kb on + strand, within DZA65_RS01250 at 277.879 kb on + strand, within DZA65_RS01250 at 277.880 kb on - strand, within DZA65_RS01250 at 277.880 kb on - strand, within DZA65_RS01250 at 277.880 kb on - strand, within DZA65_RS01250 at 277.888 kb on + strand, within DZA65_RS01250 at 277.888 kb on + strand, within DZA65_RS01250 at 277.889 kb on - strand, within DZA65_RS01250 at 277.889 kb on - strand at 277.889 kb on - strand, within DZA65_RS01250 at 277.907 kb on + strand, within DZA65_RS01250 at 277.907 kb on + strand, within DZA65_RS01250 at 277.954 kb on + strand, within DZA65_RS01250 at 277.954 kb on + strand at 277.954 kb on + strand, within DZA65_RS01250 at 277.964 kb on - strand, within DZA65_RS01250 at 278.007 kb on + strand, within DZA65_RS01250 at 278.078 kb on + strand, within DZA65_RS01250 at 278.078 kb on + strand, within DZA65_RS01250 at 278.078 kb on + strand, within DZA65_RS01250 at 278.078 kb on + strand, within DZA65_RS01250 at 278.078 kb on + strand at 278.078 kb on + strand, within DZA65_RS01250 at 278.078 kb on + strand at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.079 kb on - strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.156 kb on + strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.157 kb on - strand, within DZA65_RS01250 at 278.208 kb on + strand, within DZA65_RS01250 at 278.209 kb on - strand, within DZA65_RS01250 at 278.209 kb on - strand, within DZA65_RS01250 at 278.216 kb on + strand, within DZA65_RS01250 at 278.217 kb on - strand, within DZA65_RS01250 at 278.217 kb on - strand, within DZA65_RS01250 at 278.217 kb on - strand, within DZA65_RS01250 at 278.434 kb on + strand at 278.435 kb on - strand at 278.435 kb on - strand at 278.435 kb on - strand at 278.498 kb on + strand at 278.498 kb on + strand at 278.499 kb on - strand at 278.512 kb on + strand at 278.512 kb on + strand at 278.512 kb on + strand at 278.512 kb on + strand at 278.512 kb on + strand at 278.513 kb on - strand at 278.513 kb on - strand at 278.513 kb on - strand at 278.513 kb on - strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.671 kb on + strand at 278.694 kb on + strand at 278.781 kb on + strand, within DZA65_RS01255 at 278.781 kb on + strand, within DZA65_RS01255 at 278.781 kb on + strand, within DZA65_RS01255 at 278.781 kb on + strand, within DZA65_RS01255 at 278.781 kb on + strand, within DZA65_RS01255 at 278.782 kb on - strand, within DZA65_RS01255 at 278.782 kb on - strand, within DZA65_RS01255 at 278.786 kb on + strand, within DZA65_RS01255 at 278.786 kb on + strand, within DZA65_RS01255 at 278.787 kb on - strand, within DZA65_RS01255 at 278.787 kb on - strand, within DZA65_RS01255 at 278.787 kb on - strand, within DZA65_RS01255 at 278.834 kb on + strand, within DZA65_RS01255 at 278.949 kb on + strand, within DZA65_RS01255 at 278.949 kb on + strand, within DZA65_RS01255 at 278.950 kb on - strand at 278.950 kb on - strand, within DZA65_RS01255 at 278.950 kb on - strand, within DZA65_RS01255 at 279.076 kb on + strand, within DZA65_RS01255 at 279.076 kb on + strand, within DZA65_RS01255 at 279.076 kb on + strand, within DZA65_RS01255 at 279.076 kb on + strand, within DZA65_RS01255 at 279.076 kb on + strand, within DZA65_RS01255 at 279.076 kb on + strand, within DZA65_RS01255 at 279.077 kb on - strand, within DZA65_RS01255 at 279.077 kb on - strand, within DZA65_RS01255 at 279.077 kb on - strand, within DZA65_RS01255 at 279.119 kb on + strand, within DZA65_RS01255 at 279.119 kb on + strand, within DZA65_RS01255 at 279.119 kb on + strand, within DZA65_RS01255 at 279.119 kb on + strand, within DZA65_RS01255 at 279.119 kb on + strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.120 kb on - strand, within DZA65_RS01255 at 279.175 kb on + strand, within DZA65_RS01255 at 279.176 kb on - strand, within DZA65_RS01255 at 279.176 kb on - strand, within DZA65_RS01255 at 279.324 kb on - strand, within DZA65_RS01255 at 279.440 kb on + strand, within DZA65_RS01255 at 279.471 kb on - strand, within DZA65_RS01255 at 279.471 kb on - strand, within DZA65_RS01255 at 279.472 kb on + strand, within DZA65_RS01255 at 279.472 kb on + strand, within DZA65_RS01255 at 279.472 kb on + strand, within DZA65_RS01255 at 279.472 kb on + strand, within DZA65_RS01255 at 279.472 kb on + strand at 279.472 kb on + strand, within DZA65_RS01255 at 279.472 kb on + strand, within DZA65_RS01255 at 279.472 kb on + strand, within DZA65_RS01255 at 279.473 kb on - strand, within DZA65_RS01255 at 279.473 kb on - strand, within DZA65_RS01255 at 279.473 kb on - strand, within DZA65_RS01255 at 279.473 kb on - strand, within DZA65_RS01255 at 279.473 kb on - strand, within DZA65_RS01255 at 279.508 kb on + strand, within DZA65_RS01255 at 279.509 kb on - strand, within DZA65_RS01255 at 279.551 kb on + strand, within DZA65_RS01255 at 279.552 kb on - strand, within DZA65_RS01255 at 279.735 kb on + strand, within DZA65_RS01255 at 279.735 kb on + strand, within DZA65_RS01255 at 279.735 kb on + strand, within DZA65_RS01255 at 279.735 kb on + strand, within DZA65_RS01255 at 279.735 kb on + strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.736 kb on - strand, within DZA65_RS01255 at 279.787 kb on + strand at 279.787 kb on + strand at 279.788 kb on - strand at 279.788 kb on - strand at 279.851 kb on + strand at 279.852 kb on - strand at 279.858 kb on - strand at 279.866 kb on + strand at 279.866 kb on + strand at 279.866 kb on + strand at 279.866 kb on + strand at 279.867 kb on - strand at 279.867 kb on - strand at 279.878 kb on + strand at 279.878 kb on + strand at 279.878 kb on + strand at 279.879 kb on - strand at 279.890 kb on - strand at 279.890 kb on - strand at 279.914 kb on + strand at 280.025 kb on + strand at 280.123 kb on + strand, within DZA65_RS01260 at 280.177 kb on + strand, within DZA65_RS01260 at 280.177 kb on + strand, within DZA65_RS01260 at 280.178 kb on - strand, within DZA65_RS01260 at 280.197 kb on - strand, within DZA65_RS01260 at 280.392 kb on + strand, within DZA65_RS01260 at 280.393 kb on - strand, within DZA65_RS01260 at 280.567 kb on + strand, within DZA65_RS01260 at 280.567 kb on + strand, within DZA65_RS01260 at 280.568 kb on - strand, within DZA65_RS01260 at 280.568 kb on - strand, within DZA65_RS01260 at 280.693 kb on + strand, within DZA65_RS01260 at 280.693 kb on + strand, within DZA65_RS01260 at 280.694 kb on - strand, within DZA65_RS01260 at 280.694 kb on - strand, within DZA65_RS01260 at 280.694 kb on - strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.758 kb on + strand, within DZA65_RS01260 at 280.759 kb on - strand, within DZA65_RS01260 at 280.759 kb on - strand at 280.759 kb on - strand, within DZA65_RS01260 at 280.759 kb on - strand, within DZA65_RS01260 at 280.759 kb on - strand, within DZA65_RS01260 at 280.759 kb on - strand, within DZA65_RS01260 at 280.760 kb on + strand, within DZA65_RS01260 at 280.760 kb on + strand at 280.762 kb on + strand, within DZA65_RS01260 at 280.762 kb on + strand, within DZA65_RS01260 at 280.762 kb on + strand, within DZA65_RS01260 at 280.762 kb on + strand, within DZA65_RS01260 at 280.762 kb on + strand, within DZA65_RS01260 at 280.763 kb on - strand, within DZA65_RS01260 at 280.763 kb on - strand, within DZA65_RS01260 at 280.763 kb on - strand, within DZA65_RS01260 at 280.763 kb on - strand, within DZA65_RS01260 at 280.763 kb on - strand, within DZA65_RS01260 at 280.763 kb on - strand, within DZA65_RS01260 at 280.763 kb on - strand, within DZA65_RS01260 at 280.803 kb on - strand, within DZA65_RS01260
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 277,879 + DZA65_RS01250 0.15 +1.1 277,879 + DZA65_RS01250 0.15 +0.8 277,879 + DZA65_RS01250 0.15 -0.9 277,879 + DZA65_RS01250 0.15 -2.2 277,880 - DZA65_RS01250 0.15 -0.3 277,880 - DZA65_RS01250 0.15 -0.3 277,880 - DZA65_RS01250 0.15 +1.4 277,888 + DZA65_RS01250 0.16 -0.7 277,888 + DZA65_RS01250 0.16 -0.9 277,889 - DZA65_RS01250 0.16 -1.7 277,889 - -1.9 277,889 - DZA65_RS01250 0.16 +2.0 277,907 + DZA65_RS01250 0.19 +0.4 277,907 + DZA65_RS01250 0.19 +1.5 277,954 + DZA65_RS01250 0.25 -1.9 277,954 + -1.9 277,954 + DZA65_RS01250 0.25 -2.1 277,964 - DZA65_RS01250 0.26 -0.7 278,007 + DZA65_RS01250 0.32 +0.8 278,078 + DZA65_RS01250 0.42 -2.0 278,078 + DZA65_RS01250 0.42 -1.4 278,078 + DZA65_RS01250 0.42 -1.8 278,078 + DZA65_RS01250 0.42 +1.3 278,078 + -0.4 278,078 + DZA65_RS01250 0.42 +0.5 278,078 + -0.9 278,079 - DZA65_RS01250 0.42 -2.7 278,079 - DZA65_RS01250 0.42 +0.5 278,079 - DZA65_RS01250 0.42 -1.0 278,079 - DZA65_RS01250 0.42 -1.2 278,079 - DZA65_RS01250 0.42 -2.8 278,079 - DZA65_RS01250 0.42 -0.4 278,079 - DZA65_RS01250 0.42 -1.5 278,079 - DZA65_RS01250 0.42 -0.9 278,079 - DZA65_RS01250 0.42 -1.4 278,079 - DZA65_RS01250 0.42 +0.7 278,156 + DZA65_RS01250 0.53 -0.4 278,156 + DZA65_RS01250 0.53 -0.0 278,156 + DZA65_RS01250 0.53 +1.6 278,156 + DZA65_RS01250 0.53 -2.3 278,156 + DZA65_RS01250 0.53 -0.7 278,156 + DZA65_RS01250 0.53 -1.2 278,156 + DZA65_RS01250 0.53 +2.4 278,156 + DZA65_RS01250 0.53 -1.3 278,156 + DZA65_RS01250 0.53 +1.6 278,156 + DZA65_RS01250 0.53 +0.0 278,157 - DZA65_RS01250 0.53 +0.1 278,157 - DZA65_RS01250 0.53 -1.2 278,157 - DZA65_RS01250 0.53 -0.9 278,157 - DZA65_RS01250 0.53 -0.1 278,157 - DZA65_RS01250 0.53 -1.4 278,157 - DZA65_RS01250 0.53 -1.0 278,157 - DZA65_RS01250 0.53 -1.9 278,157 - DZA65_RS01250 0.53 -0.1 278,157 - DZA65_RS01250 0.53 -0.4 278,157 - DZA65_RS01250 0.53 -0.3 278,208 + DZA65_RS01250 0.60 +1.1 278,209 - DZA65_RS01250 0.60 -0.9 278,209 - DZA65_RS01250 0.60 -1.2 278,216 + DZA65_RS01250 0.61 -1.0 278,217 - DZA65_RS01250 0.61 -0.4 278,217 - DZA65_RS01250 0.61 -0.4 278,217 - DZA65_RS01250 0.61 -1.1 278,434 + -0.4 278,435 - -0.1 278,435 - -0.4 278,435 - -1.8 278,498 + -0.7 278,498 + +0.1 278,499 - +0.1 278,512 + -1.9 278,512 + -0.4 278,512 + +1.8 278,512 + +0.1 278,512 + -0.1 278,513 - -0.4 278,513 - -0.7 278,513 - -0.4 278,513 - -0.4 278,521 + -0.3 278,521 + -0.7 278,671 + -0.9 278,694 + -3.3 278,781 + DZA65_RS01255 0.15 -1.0 278,781 + DZA65_RS01255 0.15 -2.2 278,781 + DZA65_RS01255 0.15 -1.4 278,781 + DZA65_RS01255 0.15 -2.0 278,781 + DZA65_RS01255 0.15 -0.7 278,782 - DZA65_RS01255 0.15 -1.9 278,782 - DZA65_RS01255 0.15 -0.4 278,786 + DZA65_RS01255 0.16 -1.9 278,786 + DZA65_RS01255 0.16 -1.3 278,787 - DZA65_RS01255 0.16 -1.9 278,787 - DZA65_RS01255 0.16 -2.6 278,787 - DZA65_RS01255 0.16 -1.9 278,834 + DZA65_RS01255 0.19 -1.3 278,949 + DZA65_RS01255 0.28 -1.4 278,949 + DZA65_RS01255 0.28 -2.2 278,950 - -0.4 278,950 - DZA65_RS01255 0.28 -1.4 278,950 - DZA65_RS01255 0.28 -0.9 279,076 + DZA65_RS01255 0.38 -1.9 279,076 + DZA65_RS01255 0.38 -2.9 279,076 + DZA65_RS01255 0.38 +0.1 279,076 + DZA65_RS01255 0.38 -1.7 279,076 + DZA65_RS01255 0.38 -1.2 279,076 + DZA65_RS01255 0.38 -2.1 279,077 - DZA65_RS01255 0.38 +1.7 279,077 - DZA65_RS01255 0.38 -3.0 279,077 - DZA65_RS01255 0.38 -1.4 279,119 + DZA65_RS01255 0.42 -1.9 279,119 + DZA65_RS01255 0.42 -0.9 279,119 + DZA65_RS01255 0.42 -0.0 279,119 + DZA65_RS01255 0.42 -3.5 279,119 + DZA65_RS01255 0.42 -2.3 279,120 - DZA65_RS01255 0.42 -2.9 279,120 - DZA65_RS01255 0.42 -1.4 279,120 - DZA65_RS01255 0.42 -2.9 279,120 - DZA65_RS01255 0.42 -0.9 279,120 - DZA65_RS01255 0.42 -1.2 279,120 - DZA65_RS01255 0.42 -1.8 279,120 - DZA65_RS01255 0.42 -1.2 279,120 - DZA65_RS01255 0.42 -2.9 279,120 - DZA65_RS01255 0.42 -0.4 279,175 + DZA65_RS01255 0.46 -4.0 279,176 - DZA65_RS01255 0.46 -1.4 279,176 - DZA65_RS01255 0.46 -0.4 279,324 - DZA65_RS01255 0.57 -2.4 279,440 + DZA65_RS01255 0.66 -2.3 279,471 - DZA65_RS01255 0.69 +1.1 279,471 - DZA65_RS01255 0.69 -2.6 279,472 + DZA65_RS01255 0.69 -2.3 279,472 + DZA65_RS01255 0.69 -2.9 279,472 + DZA65_RS01255 0.69 -1.4 279,472 + DZA65_RS01255 0.69 -2.6 279,472 + +0.1 279,472 + DZA65_RS01255 0.69 -1.0 279,472 + DZA65_RS01255 0.69 -1.2 279,472 + DZA65_RS01255 0.69 -3.0 279,473 - DZA65_RS01255 0.69 -2.0 279,473 - DZA65_RS01255 0.69 -1.9 279,473 - DZA65_RS01255 0.69 -2.9 279,473 - DZA65_RS01255 0.69 +1.1 279,473 - DZA65_RS01255 0.69 -2.9 279,508 + DZA65_RS01255 0.72 -1.7 279,509 - DZA65_RS01255 0.72 -0.4 279,551 + DZA65_RS01255 0.75 -2.2 279,552 - DZA65_RS01255 0.75 -1.6 279,735 + DZA65_RS01255 0.89 -2.6 279,735 + DZA65_RS01255 0.89 -0.9 279,735 + DZA65_RS01255 0.89 +1.5 279,735 + DZA65_RS01255 0.89 -3.5 279,735 + DZA65_RS01255 0.89 +0.9 279,736 - DZA65_RS01255 0.89 -0.4 279,736 - DZA65_RS01255 0.89 -1.7 279,736 - DZA65_RS01255 0.89 -1.9 279,736 - DZA65_RS01255 0.89 -0.9 279,736 - DZA65_RS01255 0.89 -1.7 279,736 - DZA65_RS01255 0.89 -1.2 279,736 - DZA65_RS01255 0.89 -1.4 279,736 - DZA65_RS01255 0.89 -1.4 279,787 + -1.9 279,787 + -1.2 279,788 - +1.1 279,788 - -0.9 279,851 + +1.7 279,852 - -1.3 279,858 - -2.8 279,866 + -3.1 279,866 + -0.4 279,866 + -0.9 279,866 + -0.4 279,867 - -2.7 279,867 - -1.8 279,878 + +1.1 279,878 + +1.1 279,878 + -1.4 279,879 - +0.4 279,890 - +2.3 279,890 - +0.1 279,914 + +0.6 280,025 + +0.1 280,123 + DZA65_RS01260 0.15 -3.4 280,177 + DZA65_RS01260 0.18 -0.4 280,177 + DZA65_RS01260 0.18 -0.4 280,178 - DZA65_RS01260 0.18 -3.3 280,197 - DZA65_RS01260 0.19 -0.9 280,392 + DZA65_RS01260 0.32 -2.8 280,393 - DZA65_RS01260 0.32 +0.6 280,567 + DZA65_RS01260 0.43 -3.4 280,567 + DZA65_RS01260 0.43 -3.4 280,568 - DZA65_RS01260 0.43 -1.7 280,568 - DZA65_RS01260 0.43 -0.4 280,693 + DZA65_RS01260 0.51 -2.0 280,693 + DZA65_RS01260 0.51 -2.2 280,694 - DZA65_RS01260 0.51 -0.4 280,694 - DZA65_RS01260 0.51 -3.6 280,694 - DZA65_RS01260 0.51 -1.7 280,758 + DZA65_RS01260 0.55 -2.4 280,758 + DZA65_RS01260 0.55 -1.4 280,758 + DZA65_RS01260 0.55 -2.3 280,758 + DZA65_RS01260 0.55 -0.9 280,758 + DZA65_RS01260 0.55 -1.7 280,758 + DZA65_RS01260 0.55 -1.9 280,758 + DZA65_RS01260 0.55 -3.3 280,758 + DZA65_RS01260 0.55 -2.2 280,759 - DZA65_RS01260 0.55 -1.4 280,759 - -1.4 280,759 - DZA65_RS01260 0.55 -1.4 280,759 - DZA65_RS01260 0.55 -2.2 280,759 - DZA65_RS01260 0.55 -4.9 280,759 - DZA65_RS01260 0.55 -0.7 280,760 + DZA65_RS01260 0.55 -3.1 280,760 + -0.4 280,762 + DZA65_RS01260 0.55 -3.9 280,762 + DZA65_RS01260 0.55 -3.3 280,762 + DZA65_RS01260 0.55 +0.1 280,762 + DZA65_RS01260 0.55 -4.2 280,762 + DZA65_RS01260 0.55 -3.4 280,763 - DZA65_RS01260 0.55 -0.1 280,763 - DZA65_RS01260 0.55 -1.9 280,763 - DZA65_RS01260 0.55 -2.9 280,763 - DZA65_RS01260 0.55 -5.1 280,763 - DZA65_RS01260 0.55 -0.4 280,763 - DZA65_RS01260 0.55 -3.1 280,763 - DZA65_RS01260 0.55 -1.2 280,803 - DZA65_RS01260 0.58 -2.3
Or see this region's nucleotide sequence