Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS04535 and DZA65_RS04540 are separated by 5 nucleotides DZA65_RS04540 and DZA65_RS04545 are separated by 0 nucleotides DZA65_RS04545 and DZA65_RS04550 are separated by 691 nucleotides
DZA65_RS04535: DZA65_RS04535 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 945,035 to 945,877
_RS04535
DZA65_RS04540: DZA65_RS04540 - queuosine precursor transporter, at 945,883 to 946,758
_RS04540
DZA65_RS04545: DZA65_RS04545 - helix-turn-helix transcriptional regulator, at 946,759 to 947,373
_RS04545
DZA65_RS04550: DZA65_RS04550 - rhamnogalacturonate lyase, at 948,065 to 949,795
_RS04550
Position (kb) 946
947
948 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 945.813 kb on + strand at 945.813 kb on + strand at 945.850 kb on + strand at 945.857 kb on - strand at 945.857 kb on - strand at 945.868 kb on + strand at 945.868 kb on + strand at 945.868 kb on + strand at 945.869 kb on - strand at 945.871 kb on + strand at 945.871 kb on + strand at 945.871 kb on + strand at 945.871 kb on + strand at 945.871 kb on + strand at 945.871 kb on + strand at 945.871 kb on + strand at 945.872 kb on - strand at 945.872 kb on - strand at 945.872 kb on - strand at 945.872 kb on - strand at 945.872 kb on - strand at 945.872 kb on - strand at 945.895 kb on + strand at 945.896 kb on - strand at 945.896 kb on - strand at 945.896 kb on - strand at 945.896 kb on - strand at 945.898 kb on + strand at 945.898 kb on + strand at 945.898 kb on + strand at 945.899 kb on - strand at 945.918 kb on - strand at 945.918 kb on - strand at 945.918 kb on - strand at 945.944 kb on + strand at 945.944 kb on + strand at 945.945 kb on - strand at 945.945 kb on - strand at 945.945 kb on - strand at 945.945 kb on - strand at 945.945 kb on - strand at 945.945 kb on - strand at 945.971 kb on + strand, within DZA65_RS04540 at 945.972 kb on - strand, within DZA65_RS04540 at 945.972 kb on - strand, within DZA65_RS04540 at 945.972 kb on - strand, within DZA65_RS04540 at 946.035 kb on + strand, within DZA65_RS04540 at 946.035 kb on + strand, within DZA65_RS04540 at 946.035 kb on + strand, within DZA65_RS04540 at 946.035 kb on + strand, within DZA65_RS04540 at 946.036 kb on - strand, within DZA65_RS04540 at 946.036 kb on - strand, within DZA65_RS04540 at 946.038 kb on - strand, within DZA65_RS04540 at 946.107 kb on + strand, within DZA65_RS04540 at 946.182 kb on - strand, within DZA65_RS04540 at 946.182 kb on - strand, within DZA65_RS04540 at 946.182 kb on - strand, within DZA65_RS04540 at 946.235 kb on + strand, within DZA65_RS04540 at 946.236 kb on - strand at 946.236 kb on - strand, within DZA65_RS04540 at 946.386 kb on + strand at 946.386 kb on + strand, within DZA65_RS04540 at 946.387 kb on - strand, within DZA65_RS04540 at 946.387 kb on - strand, within DZA65_RS04540 at 946.387 kb on - strand, within DZA65_RS04540 at 946.429 kb on - strand, within DZA65_RS04540 at 946.429 kb on - strand, within DZA65_RS04540 at 946.443 kb on + strand, within DZA65_RS04540 at 946.443 kb on + strand, within DZA65_RS04540 at 946.443 kb on + strand, within DZA65_RS04540 at 946.443 kb on + strand, within DZA65_RS04540 at 946.443 kb on + strand, within DZA65_RS04540 at 946.444 kb on - strand, within DZA65_RS04540 at 946.444 kb on - strand, within DZA65_RS04540 at 946.444 kb on - strand, within DZA65_RS04540 at 946.444 kb on - strand, within DZA65_RS04540 at 946.444 kb on - strand, within DZA65_RS04540 at 946.481 kb on + strand, within DZA65_RS04540 at 946.482 kb on - strand, within DZA65_RS04540 at 946.514 kb on + strand, within DZA65_RS04540 at 946.553 kb on + strand, within DZA65_RS04540 at 946.554 kb on - strand, within DZA65_RS04540 at 946.580 kb on + strand, within DZA65_RS04540 at 946.581 kb on - strand, within DZA65_RS04540 at 946.685 kb on - strand at 946.687 kb on + strand at 946.687 kb on + strand at 946.687 kb on + strand at 946.687 kb on + strand at 946.687 kb on + strand at 946.688 kb on - strand at 946.688 kb on - strand at 946.688 kb on - strand at 946.688 kb on - strand at 946.760 kb on + strand at 946.760 kb on + strand at 946.760 kb on + strand at 946.760 kb on + strand at 946.760 kb on + strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.761 kb on - strand at 946.762 kb on + strand at 946.762 kb on + strand at 946.774 kb on + strand at 946.775 kb on - strand at 946.775 kb on - strand at 946.775 kb on - strand at 946.775 kb on - strand at 946.775 kb on - strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.779 kb on + strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.780 kb on - strand at 946.781 kb on + strand at 946.781 kb on + strand at 946.781 kb on + strand at 946.782 kb on - strand at 946.782 kb on - strand at 946.782 kb on - strand at 946.980 kb on + strand, within DZA65_RS04545 at 946.980 kb on + strand, within DZA65_RS04545 at 946.981 kb on - strand, within DZA65_RS04545 at 947.003 kb on + strand, within DZA65_RS04545 at 947.003 kb on + strand, within DZA65_RS04545 at 947.003 kb on + strand, within DZA65_RS04545 at 947.004 kb on - strand, within DZA65_RS04545 at 947.121 kb on + strand, within DZA65_RS04545 at 947.121 kb on + strand, within DZA65_RS04545 at 947.121 kb on + strand, within DZA65_RS04545 at 947.121 kb on + strand, within DZA65_RS04545 at 947.121 kb on + strand, within DZA65_RS04545 at 947.122 kb on - strand, within DZA65_RS04545 at 947.122 kb on - strand, within DZA65_RS04545 at 947.153 kb on + strand, within DZA65_RS04545 at 947.153 kb on + strand, within DZA65_RS04545 at 947.153 kb on + strand, within DZA65_RS04545 at 947.153 kb on + strand, within DZA65_RS04545 at 947.153 kb on + strand, within DZA65_RS04545 at 947.153 kb on + strand, within DZA65_RS04545 at 947.154 kb on - strand, within DZA65_RS04545 at 947.154 kb on - strand, within DZA65_RS04545 at 947.154 kb on - strand, within DZA65_RS04545 at 947.228 kb on - strand, within DZA65_RS04545 at 947.228 kb on - strand, within DZA65_RS04545 at 947.232 kb on + strand, within DZA65_RS04545 at 947.232 kb on + strand, within DZA65_RS04545 at 947.232 kb on + strand, within DZA65_RS04545 at 947.253 kb on + strand, within DZA65_RS04545 at 947.253 kb on + strand, within DZA65_RS04545 at 947.253 kb on + strand, within DZA65_RS04545 at 947.253 kb on + strand, within DZA65_RS04545 at 947.253 kb on + strand, within DZA65_RS04545 at 947.254 kb on - strand, within DZA65_RS04545 at 947.260 kb on - strand, within DZA65_RS04545 at 947.260 kb on - strand, within DZA65_RS04545 at 947.274 kb on + strand, within DZA65_RS04545 at 947.274 kb on + strand, within DZA65_RS04545 at 947.274 kb on + strand, within DZA65_RS04545 at 947.310 kb on + strand, within DZA65_RS04545 at 947.317 kb on + strand at 947.317 kb on + strand at 947.317 kb on + strand at 947.317 kb on + strand at 947.317 kb on + strand at 947.317 kb on + strand at 947.317 kb on + strand at 947.318 kb on - strand at 947.318 kb on - strand at 947.318 kb on - strand at 947.318 kb on - strand at 947.321 kb on + strand at 947.322 kb on - strand at 947.329 kb on - strand at 947.335 kb on - strand at 947.340 kb on + strand at 947.341 kb on - strand at 947.343 kb on + strand at 947.343 kb on + strand at 947.343 kb on + strand at 947.415 kb on - strand at 947.420 kb on - strand at 947.427 kb on + strand at 947.427 kb on + strand at 947.427 kb on + strand at 947.427 kb on + strand at 947.428 kb on - strand at 947.437 kb on + strand at 947.476 kb on + strand at 947.487 kb on + strand at 947.487 kb on + strand at 947.488 kb on - strand at 947.488 kb on - strand at 947.488 kb on - strand at 947.488 kb on - strand at 947.488 kb on - strand at 947.521 kb on + strand at 947.522 kb on - strand at 947.522 kb on - strand at 947.529 kb on + strand at 947.529 kb on + strand at 947.530 kb on - strand at 947.579 kb on + strand at 947.579 kb on + strand at 947.580 kb on - strand at 947.580 kb on - strand at 947.580 kb on - strand at 947.580 kb on - strand at 947.582 kb on + strand at 947.582 kb on + strand at 947.582 kb on + strand at 947.582 kb on + strand at 947.582 kb on + strand at 947.582 kb on + strand at 947.582 kb on + strand at 947.583 kb on - strand at 947.583 kb on - strand at 947.583 kb on - strand at 947.583 kb on - strand at 947.583 kb on - strand at 947.586 kb on + strand at 947.586 kb on + strand at 947.586 kb on + strand at 947.587 kb on - strand at 947.587 kb on - strand at 947.591 kb on + strand at 947.591 kb on + strand at 947.614 kb on + strand at 947.639 kb on - strand at 947.670 kb on + strand at 947.674 kb on + strand at 947.674 kb on + strand at 947.674 kb on + strand at 947.676 kb on + strand at 947.682 kb on - strand at 947.683 kb on + strand at 947.684 kb on - strand at 947.691 kb on - strand at 947.691 kb on - strand at 947.736 kb on - strand at 947.767 kb on + strand at 947.767 kb on + strand at 947.768 kb on - strand at 947.768 kb on - strand at 947.786 kb on + strand at 947.787 kb on - strand at 947.800 kb on + strand at 947.800 kb on + strand at 947.807 kb on + strand at 947.807 kb on + strand at 947.808 kb on - strand at 947.808 kb on - strand at 947.811 kb on + strand at 947.859 kb on - strand at 947.859 kb on - strand at 947.900 kb on + strand at 947.901 kb on - strand at 947.901 kb on - strand at 947.901 kb on - strand at 947.901 kb on - strand at 947.901 kb on - strand at 947.901 kb on - strand at 947.951 kb on + strand at 947.951 kb on + strand at 947.952 kb on - strand at 947.952 kb on - strand at 947.963 kb on + strand at 947.963 kb on + strand at 947.964 kb on - strand at 947.964 kb on - strand at 947.964 kb on - strand at 947.967 kb on + strand at 947.967 kb on + strand at 947.967 kb on + strand at 947.967 kb on + strand at 947.967 kb on + strand at 947.968 kb on - strand at 947.968 kb on - strand at 947.969 kb on + strand at 947.969 kb on + strand at 947.970 kb on - strand at 947.970 kb on - strand at 947.970 kb on - strand at 947.970 kb on - strand at 947.979 kb on + strand at 947.979 kb on + strand at 947.980 kb on - strand at 947.983 kb on + strand at 947.983 kb on + strand at 947.983 kb on + strand at 947.983 kb on + strand at 947.984 kb on - strand at 947.984 kb on - strand at 947.984 kb on - strand at 947.984 kb on - strand at 947.996 kb on - strand at 947.997 kb on + strand at 947.997 kb on + strand at 947.997 kb on + strand at 947.998 kb on - strand at 948.099 kb on + strand at 948.099 kb on + strand at 948.099 kb on + strand at 948.099 kb on + strand at 948.100 kb on - strand at 948.100 kb on - strand at 948.100 kb on - strand at 948.100 kb on - strand at 948.100 kb on - strand at 948.152 kb on + strand at 948.152 kb on + strand at 948.153 kb on - strand at 948.175 kb on + strand at 948.238 kb on - strand, within DZA65_RS04550 at 948.238 kb on - strand, within DZA65_RS04550 at 948.238 kb on - strand, within DZA65_RS04550 at 948.348 kb on + strand, within DZA65_RS04550 at 948.348 kb on + strand, within DZA65_RS04550 at 948.349 kb on - strand, within DZA65_RS04550 at 948.349 kb on - strand, within DZA65_RS04550 at 948.349 kb on - strand, within DZA65_RS04550 at 948.349 kb on - strand, within DZA65_RS04550 at 948.349 kb on - strand, within DZA65_RS04550 at 948.349 kb on - strand, within DZA65_RS04550 at 948.349 kb on - strand, within DZA65_RS04550
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 945,813 + +0.4 945,813 + +1.8 945,850 + -0.0 945,857 - +1.7 945,857 - +0.6 945,868 + -1.8 945,868 + -1.3 945,868 + +0.3 945,869 - +1.7 945,871 + +1.4 945,871 + -1.3 945,871 + +1.7 945,871 + +1.8 945,871 + +0.6 945,871 + +3.0 945,871 + +0.8 945,872 - -0.6 945,872 - +1.3 945,872 - -0.6 945,872 - +2.7 945,872 - +4.9 945,872 - -0.8 945,895 + -0.6 945,896 - -0.3 945,896 - +1.4 945,896 - +0.2 945,896 - -1.5 945,898 + -0.2 945,898 + +1.6 945,898 + -0.6 945,899 - -0.4 945,918 - +0.5 945,918 - +0.4 945,918 - +0.7 945,944 + +0.7 945,944 + +0.9 945,945 - +1.1 945,945 - -2.0 945,945 - -3.1 945,945 - -1.8 945,945 - -0.2 945,945 - +0.9 945,971 + DZA65_RS04540 0.10 +1.2 945,972 - DZA65_RS04540 0.10 -2.4 945,972 - DZA65_RS04540 0.10 -2.7 945,972 - DZA65_RS04540 0.10 +1.4 946,035 + DZA65_RS04540 0.17 +1.8 946,035 + DZA65_RS04540 0.17 +1.7 946,035 + DZA65_RS04540 0.17 +2.1 946,035 + DZA65_RS04540 0.17 +1.4 946,036 - DZA65_RS04540 0.17 -0.6 946,036 - DZA65_RS04540 0.17 +2.1 946,038 - DZA65_RS04540 0.18 -1.3 946,107 + DZA65_RS04540 0.26 -0.2 946,182 - DZA65_RS04540 0.34 +0.3 946,182 - DZA65_RS04540 0.34 -2.0 946,182 - DZA65_RS04540 0.34 -0.4 946,235 + DZA65_RS04540 0.40 -0.3 946,236 - +0.4 946,236 - DZA65_RS04540 0.40 +0.4 946,386 + -1.3 946,386 + DZA65_RS04540 0.57 -0.8 946,387 - DZA65_RS04540 0.58 +0.5 946,387 - DZA65_RS04540 0.58 -1.7 946,387 - DZA65_RS04540 0.58 +1.2 946,429 - DZA65_RS04540 0.62 -0.2 946,429 - DZA65_RS04540 0.62 -0.0 946,443 + DZA65_RS04540 0.64 -1.8 946,443 + DZA65_RS04540 0.64 -1.6 946,443 + DZA65_RS04540 0.64 -0.8 946,443 + DZA65_RS04540 0.64 +1.5 946,443 + DZA65_RS04540 0.64 -1.8 946,444 - DZA65_RS04540 0.64 -1.3 946,444 - DZA65_RS04540 0.64 +0.2 946,444 - DZA65_RS04540 0.64 +0.4 946,444 - DZA65_RS04540 0.64 -0.4 946,444 - DZA65_RS04540 0.64 -0.2 946,481 + DZA65_RS04540 0.68 -0.6 946,482 - DZA65_RS04540 0.68 -2.2 946,514 + DZA65_RS04540 0.72 -2.2 946,553 + DZA65_RS04540 0.76 -0.0 946,554 - DZA65_RS04540 0.77 -0.0 946,580 + DZA65_RS04540 0.80 +0.6 946,581 - DZA65_RS04540 0.80 -1.6 946,685 - +1.6 946,687 + -0.3 946,687 + -1.8 946,687 + +2.4 946,687 + +2.0 946,687 + +0.4 946,688 - +0.1 946,688 - +2.0 946,688 - -0.4 946,688 - +0.2 946,760 + +0.3 946,760 + +0.4 946,760 + -0.3 946,760 + -1.8 946,760 + -0.6 946,761 - -1.7 946,761 - -1.2 946,761 - +1.2 946,761 - -0.9 946,761 - +1.2 946,761 - -0.0 946,761 - +0.6 946,761 - -0.0 946,761 - +1.0 946,762 + +1.0 946,762 + -2.0 946,774 + -0.6 946,775 - +0.8 946,775 - -1.0 946,775 - +0.2 946,775 - -1.0 946,775 - -2.2 946,779 + -1.6 946,779 + +1.5 946,779 + +1.0 946,779 + -0.6 946,779 + -1.8 946,779 + -0.0 946,779 + -2.5 946,779 + -0.6 946,779 + -0.6 946,779 + +0.4 946,779 + -0.8 946,779 + +1.0 946,779 + +0.5 946,779 + -0.2 946,779 + -0.6 946,779 + +1.3 946,779 + -0.6 946,779 + -0.7 946,779 + -0.6 946,779 + +2.6 946,779 + +0.3 946,780 - +0.1 946,780 - +1.2 946,780 - -0.5 946,780 - -0.4 946,780 - -0.1 946,780 - +0.4 946,780 - +1.4 946,780 - +0.9 946,780 - -0.3 946,780 - -0.0 946,780 - +0.9 946,780 - +0.1 946,780 - +2.2 946,780 - +1.0 946,780 - +1.8 946,780 - +1.7 946,780 - +0.5 946,780 - +1.4 946,780 - +0.5 946,780 - -0.0 946,780 - -1.6 946,780 - +1.7 946,780 - -1.3 946,780 - +0.6 946,780 - +0.7 946,780 - +0.4 946,780 - -2.2 946,780 - -0.3 946,780 - -1.3 946,781 + -2.5 946,781 + -1.2 946,781 + -0.7 946,782 - -1.5 946,782 - -0.5 946,782 - -0.0 946,980 + DZA65_RS04545 0.36 -1.0 946,980 + DZA65_RS04545 0.36 +2.1 946,981 - DZA65_RS04545 0.36 +2.1 947,003 + DZA65_RS04545 0.40 +0.8 947,003 + DZA65_RS04545 0.40 +1.7 947,003 + DZA65_RS04545 0.40 +1.5 947,004 - DZA65_RS04545 0.40 +2.9 947,121 + DZA65_RS04545 0.59 -2.3 947,121 + DZA65_RS04545 0.59 +0.7 947,121 + DZA65_RS04545 0.59 +1.4 947,121 + DZA65_RS04545 0.59 +0.8 947,121 + DZA65_RS04545 0.59 -1.5 947,122 - DZA65_RS04545 0.59 -2.0 947,122 - DZA65_RS04545 0.59 +1.2 947,153 + DZA65_RS04545 0.64 -0.5 947,153 + DZA65_RS04545 0.64 -0.0 947,153 + DZA65_RS04545 0.64 -0.2 947,153 + DZA65_RS04545 0.64 -0.8 947,153 + DZA65_RS04545 0.64 +1.7 947,153 + DZA65_RS04545 0.64 +1.0 947,154 - DZA65_RS04545 0.64 +1.2 947,154 - DZA65_RS04545 0.64 -0.0 947,154 - DZA65_RS04545 0.64 +1.3 947,228 - DZA65_RS04545 0.76 -0.3 947,228 - DZA65_RS04545 0.76 +1.0 947,232 + DZA65_RS04545 0.77 -1.7 947,232 + DZA65_RS04545 0.77 -0.2 947,232 + DZA65_RS04545 0.77 +1.7 947,253 + DZA65_RS04545 0.80 +0.4 947,253 + DZA65_RS04545 0.80 -0.4 947,253 + DZA65_RS04545 0.80 +0.8 947,253 + DZA65_RS04545 0.80 +1.2 947,253 + DZA65_RS04545 0.80 +0.7 947,254 - DZA65_RS04545 0.80 +1.2 947,260 - DZA65_RS04545 0.81 +1.2 947,260 - DZA65_RS04545 0.81 +0.6 947,274 + DZA65_RS04545 0.84 +0.9 947,274 + DZA65_RS04545 0.84 +0.3 947,274 + DZA65_RS04545 0.84 +0.7 947,310 + DZA65_RS04545 0.90 -2.3 947,317 + +2.4 947,317 + +0.9 947,317 + +1.8 947,317 + +1.6 947,317 + -0.9 947,317 + +0.6 947,317 + -0.6 947,318 - -1.6 947,318 - +1.7 947,318 - +1.4 947,318 - +2.3 947,321 + +0.6 947,322 - +0.2 947,329 - -1.0 947,335 - +0.7 947,340 + +2.1 947,341 - +0.7 947,343 + -1.1 947,343 + +0.8 947,343 + +1.2 947,415 - +0.8 947,420 - -0.6 947,427 + -1.2 947,427 + -2.2 947,427 + -1.2 947,427 + +0.7 947,428 - 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DZA65_RS04550 0.16 +1.1 948,349 - DZA65_RS04550 0.16 +0.2 948,349 - DZA65_RS04550 0.16 -1.2 948,349 - DZA65_RS04550 0.16 +1.0 948,349 - DZA65_RS04550 0.16 -0.3 948,349 - DZA65_RS04550 0.16 -0.6 948,349 - DZA65_RS04550 0.16 -0.9
Or see this region's nucleotide sequence