Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03500 and DZA65_RS03505 are separated by 9 nucleotides DZA65_RS03505 and DZA65_RS03510 are separated by 147 nucleotides DZA65_RS03510 and DZA65_RS03515 are separated by 85 nucleotides
DZA65_RS03500: DZA65_RS03500 - peptide chain release factor 2, at 739,633 to 740,731
_RS03500
DZA65_RS03505: DZA65_RS03505 - lysine--tRNA ligase, at 740,741 to 742,258
_RS03505
DZA65_RS03510: DZA65_RS03510 - LysE family transporter, at 742,406 to 743,077
_RS03510
DZA65_RS03515: DZA65_RS03515 - tRNA-Gly, at 743,163 to 743,236
_RS03515
Position (kb) 740
741
742
743 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 742.256 kb on + strand at 742.257 kb on - strand at 742.271 kb on + strand at 742.271 kb on + strand at 742.271 kb on + strand at 742.271 kb on + strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.272 kb on - strand at 742.273 kb on + strand at 742.274 kb on - strand at 742.274 kb on - strand at 742.324 kb on + strand at 742.324 kb on + strand at 742.324 kb on + strand at 742.324 kb on + strand at 742.325 kb on - strand at 742.325 kb on - strand at 742.325 kb on - strand at 742.325 kb on - strand at 742.329 kb on + strand at 742.329 kb on + strand at 742.329 kb on + strand at 742.367 kb on + strand at 742.367 kb on + strand at 742.367 kb on + strand at 742.367 kb on + strand at 742.367 kb on + strand at 742.368 kb on - strand at 742.368 kb on - strand at 742.368 kb on - strand at 742.368 kb on - strand at 742.371 kb on + strand at 742.371 kb on + strand at 742.372 kb on - strand at 742.372 kb on - strand at 742.382 kb on + strand at 742.383 kb on - strand at 742.394 kb on - strand at 742.394 kb on - strand at 742.394 kb on - strand at 742.405 kb on + strand at 742.406 kb on - strand at 742.484 kb on - strand, within DZA65_RS03510 at 742.484 kb on - strand, within DZA65_RS03510 at 742.486 kb on + strand, within DZA65_RS03510 at 742.486 kb on + strand, within DZA65_RS03510 at 742.486 kb on + strand, within DZA65_RS03510 at 742.486 kb on + strand, within DZA65_RS03510 at 742.487 kb on - strand, within DZA65_RS03510 at 742.487 kb on - strand, within DZA65_RS03510 at 742.525 kb on + strand, within DZA65_RS03510 at 742.559 kb on + strand, within DZA65_RS03510 at 742.560 kb on - strand, within DZA65_RS03510 at 742.560 kb on - strand, within DZA65_RS03510 at 742.601 kb on + strand, within DZA65_RS03510 at 742.601 kb on + strand, within DZA65_RS03510 at 742.601 kb on + strand, within DZA65_RS03510 at 742.601 kb on + strand, within DZA65_RS03510 at 742.601 kb on + strand, within DZA65_RS03510 at 742.601 kb on + strand, within DZA65_RS03510 at 742.601 kb on + strand, within DZA65_RS03510 at 742.602 kb on - strand, within DZA65_RS03510 at 742.602 kb on - strand, within DZA65_RS03510 at 742.603 kb on + strand, within DZA65_RS03510 at 742.603 kb on + strand, within DZA65_RS03510 at 742.603 kb on + strand, within DZA65_RS03510 at 742.603 kb on + strand, within DZA65_RS03510 at 742.604 kb on - strand, within DZA65_RS03510 at 742.604 kb on - strand, within DZA65_RS03510 at 742.604 kb on - strand, within DZA65_RS03510 at 742.624 kb on - strand, within DZA65_RS03510 at 742.644 kb on + strand, within DZA65_RS03510 at 742.644 kb on + strand, within DZA65_RS03510 at 742.644 kb on + strand, within DZA65_RS03510 at 742.644 kb on + strand, within DZA65_RS03510 at 742.644 kb on + strand, within DZA65_RS03510 at 742.645 kb on - strand, within DZA65_RS03510 at 742.645 kb on - strand, within DZA65_RS03510 at 742.645 kb on - strand, within DZA65_RS03510 at 742.732 kb on - strand, within DZA65_RS03510 at 742.772 kb on - strand, within DZA65_RS03510 at 742.878 kb on + strand, within DZA65_RS03510 at 742.879 kb on - strand, within DZA65_RS03510 at 742.879 kb on - strand, within DZA65_RS03510 at 742.879 kb on - strand, within DZA65_RS03510 at 742.879 kb on - strand, within DZA65_RS03510 at 742.879 kb on - strand, within DZA65_RS03510 at 742.941 kb on + strand, within DZA65_RS03510 at 742.941 kb on + strand, within DZA65_RS03510 at 742.985 kb on + strand, within DZA65_RS03510 at 742.985 kb on + strand, within DZA65_RS03510 at 742.986 kb on - strand, within DZA65_RS03510 at 743.011 kb on - strand at 743.011 kb on - strand at 743.011 kb on - strand at 743.011 kb on - strand at 743.011 kb on - strand at 743.089 kb on - strand at 743.089 kb on - strand at 743.089 kb on - strand at 743.089 kb on - strand at 743.141 kb on + strand at 743.141 kb on + strand at 743.141 kb on + strand at 743.141 kb on + strand at 743.142 kb on - strand at 743.142 kb on - strand at 743.142 kb on - strand at 743.142 kb on - strand at 743.142 kb on - strand at 743.142 kb on - strand at 743.142 kb on - strand at 743.142 kb on - strand at 743.143 kb on + strand at 743.143 kb on + strand at 743.143 kb on + strand at 743.144 kb on - strand at 743.144 kb on - strand at 743.144 kb on - strand at 743.182 kb on - strand, within DZA65_RS03515 at 743.204 kb on + strand, within DZA65_RS03515 at 743.204 kb on + strand, within DZA65_RS03515 at 743.204 kb on + strand, within DZA65_RS03515 at 743.205 kb on - strand, within DZA65_RS03515 at 743.205 kb on - strand, within DZA65_RS03515 at 743.206 kb on + strand, within DZA65_RS03515 at 743.206 kb on + strand, within DZA65_RS03515
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 742,256 + +0.3 742,257 - +0.5 742,271 + -2.3 742,271 + -1.8 742,271 + +1.0 742,271 + -1.7 742,272 - +1.0 742,272 - +0.0 742,272 - -0.2 742,272 - -1.0 742,272 - +0.2 742,272 - -0.6 742,272 - +1.0 742,272 - -0.5 742,272 - -1.3 742,273 + -1.3 742,274 - -2.0 742,274 - +1.6 742,324 + +0.1 742,324 + -1.3 742,324 + -1.5 742,324 + +0.0 742,325 - +0.3 742,325 - +1.5 742,325 - +1.0 742,325 - -1.8 742,329 + +0.2 742,329 + -1.5 742,329 + -0.7 742,367 + +1.1 742,367 + -0.1 742,367 + -2.2 742,367 + +0.0 742,367 + +0.2 742,368 - +0.7 742,368 - +0.6 742,368 - -1.0 742,368 - +0.0 742,371 + +1.7 742,371 + +0.8 742,372 - -3.0 742,372 - -1.0 742,382 + -1.3 742,383 - +1.4 742,394 - +0.2 742,394 - +0.2 742,394 - -0.2 742,405 + -1.0 742,406 - -0.6 742,484 - DZA65_RS03510 0.12 -0.1 742,484 - DZA65_RS03510 0.12 -1.1 742,486 + DZA65_RS03510 0.12 +1.6 742,486 + DZA65_RS03510 0.12 +1.5 742,486 + DZA65_RS03510 0.12 +3.2 742,486 + DZA65_RS03510 0.12 -0.3 742,487 - DZA65_RS03510 0.12 +0.0 742,487 - DZA65_RS03510 0.12 -0.1 742,525 + DZA65_RS03510 0.18 +2.2 742,559 + DZA65_RS03510 0.23 +0.6 742,560 - DZA65_RS03510 0.23 -2.0 742,560 - DZA65_RS03510 0.23 -1.9 742,601 + DZA65_RS03510 0.29 -0.5 742,601 + DZA65_RS03510 0.29 -1.8 742,601 + DZA65_RS03510 0.29 +0.6 742,601 + DZA65_RS03510 0.29 -0.8 742,601 + DZA65_RS03510 0.29 -2.7 742,601 + DZA65_RS03510 0.29 -1.5 742,601 + DZA65_RS03510 0.29 -0.1 742,602 - DZA65_RS03510 0.29 +0.3 742,602 - DZA65_RS03510 0.29 -0.2 742,603 + DZA65_RS03510 0.29 -2.4 742,603 + DZA65_RS03510 0.29 -1.2 742,603 + DZA65_RS03510 0.29 -0.4 742,603 + DZA65_RS03510 0.29 -2.5 742,604 - DZA65_RS03510 0.29 -0.4 742,604 - DZA65_RS03510 0.29 -0.0 742,604 - DZA65_RS03510 0.29 -1.0 742,624 - DZA65_RS03510 0.32 +0.5 742,644 + DZA65_RS03510 0.35 -0.6 742,644 + DZA65_RS03510 0.35 -1.5 742,644 + DZA65_RS03510 0.35 -1.5 742,644 + DZA65_RS03510 0.35 -0.3 742,644 + DZA65_RS03510 0.35 -2.1 742,645 - DZA65_RS03510 0.36 -0.8 742,645 - DZA65_RS03510 0.36 +1.5 742,645 - DZA65_RS03510 0.36 +0.5 742,732 - DZA65_RS03510 0.49 -0.5 742,772 - DZA65_RS03510 0.54 +1.0 742,878 + DZA65_RS03510 0.70 -1.0 742,879 - DZA65_RS03510 0.70 +0.7 742,879 - DZA65_RS03510 0.70 +1.6 742,879 - DZA65_RS03510 0.70 +0.6 742,879 - DZA65_RS03510 0.70 -1.0 742,879 - DZA65_RS03510 0.70 -0.8 742,941 + DZA65_RS03510 0.80 -1.3 742,941 + DZA65_RS03510 0.80 -2.0 742,985 + DZA65_RS03510 0.86 +0.0 742,985 + DZA65_RS03510 0.86 +2.0 742,986 - DZA65_RS03510 0.86 +1.3 743,011 - -1.5 743,011 - -0.5 743,011 - +2.2 743,011 - +2.6 743,011 - +2.4 743,089 - -0.2 743,089 - -1.3 743,089 - +0.3 743,089 - +1.3 743,141 + +0.3 743,141 + -0.8 743,141 + +1.8 743,141 + +0.5 743,142 - -2.5 743,142 - +2.1 743,142 - +0.0 743,142 - -1.3 743,142 - +0.9 743,142 - +5.0 743,142 - -0.8 743,142 - +1.4 743,143 + +0.8 743,143 + -0.6 743,143 + -2.1 743,144 - -2.7 743,144 - +2.6 743,144 - +0.5 743,182 - DZA65_RS03515 0.26 -0.5 743,204 + DZA65_RS03515 0.55 +0.7 743,204 + DZA65_RS03515 0.55 -0.5 743,204 + DZA65_RS03515 0.55 +0.5 743,205 - DZA65_RS03515 0.57 +0.5 743,205 - DZA65_RS03515 0.57 -0.6 743,206 + DZA65_RS03515 0.58 -1.0 743,206 + DZA65_RS03515 0.58 +2.3
Or see this region's nucleotide sequence