Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10980 and DZA65_RS10985 are separated by 66 nucleotides DZA65_RS10985 and DZA65_RS10990 are separated by 21 nucleotides DZA65_RS10990 and DZA65_RS10995 are separated by 282 nucleotides
DZA65_RS10980: DZA65_RS10980 - 23S rRNA pseudouridine(2605) synthase RluB, at 2,323,009 to 2,323,899
_RS10980
DZA65_RS10985: DZA65_RS10985 - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 2,323,966 to 2,324,556
_RS10985
DZA65_RS10990: DZA65_RS10990 - YciK family oxidoreductase, at 2,324,578 to 2,325,348
_RS10990
DZA65_RS10995: DZA65_RS10995 - protease SohB, at 2,325,631 to 2,326,677
_RS10995
Position (kb)
2324
2325
2326 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2323.724 kb on - strand, within DZA65_RS10980 at 2323.898 kb on - strand at 2323.900 kb on + strand at 2323.901 kb on - strand at 2323.901 kb on - strand at 2323.908 kb on + strand at 2323.908 kb on + strand at 2323.909 kb on - strand at 2323.909 kb on - strand at 2323.909 kb on - strand at 2323.909 kb on - strand at 2323.977 kb on + strand at 2324.147 kb on + strand, within DZA65_RS10985 at 2324.147 kb on + strand, within DZA65_RS10985 at 2324.148 kb on - strand, within DZA65_RS10985 at 2324.148 kb on - strand, within DZA65_RS10985 at 2324.148 kb on - strand, within DZA65_RS10985 at 2324.154 kb on - strand, within DZA65_RS10985 at 2324.154 kb on - strand, within DZA65_RS10985 at 2324.154 kb on - strand, within DZA65_RS10985 at 2324.154 kb on - strand, within DZA65_RS10985 at 2324.165 kb on + strand, within DZA65_RS10985 at 2324.165 kb on + strand, within DZA65_RS10985 at 2324.165 kb on + strand, within DZA65_RS10985 at 2324.165 kb on + strand, within DZA65_RS10985 at 2324.165 kb on + strand, within DZA65_RS10985 at 2324.165 kb on + strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.166 kb on - strand, within DZA65_RS10985 at 2324.461 kb on + strand, within DZA65_RS10985 at 2324.461 kb on + strand, within DZA65_RS10985 at 2324.462 kb on - strand, within DZA65_RS10985 at 2324.462 kb on - strand, within DZA65_RS10985 at 2324.657 kb on - strand, within DZA65_RS10990 at 2324.657 kb on - strand, within DZA65_RS10990 at 2324.661 kb on - strand, within DZA65_RS10990 at 2324.661 kb on - strand, within DZA65_RS10990 at 2324.666 kb on + strand, within DZA65_RS10990 at 2324.666 kb on + strand, within DZA65_RS10990 at 2324.666 kb on + strand, within DZA65_RS10990 at 2324.666 kb on + strand, within DZA65_RS10990 at 2324.770 kb on - strand, within DZA65_RS10990 at 2324.770 kb on - strand, within DZA65_RS10990 at 2324.953 kb on - strand, within DZA65_RS10990 at 2324.953 kb on - strand, within DZA65_RS10990 at 2324.953 kb on - strand, within DZA65_RS10990 at 2324.953 kb on - strand, within DZA65_RS10990 at 2325.054 kb on + strand, within DZA65_RS10990 at 2325.054 kb on + strand, within DZA65_RS10990 at 2325.054 kb on + strand, within DZA65_RS10990 at 2325.054 kb on + strand, within DZA65_RS10990 at 2325.055 kb on - strand, within DZA65_RS10990 at 2325.055 kb on - strand, within DZA65_RS10990 at 2325.138 kb on + strand, within DZA65_RS10990 at 2325.138 kb on + strand, within DZA65_RS10990 at 2325.138 kb on + strand, within DZA65_RS10990 at 2325.155 kb on + strand, within DZA65_RS10990 at 2325.156 kb on - strand, within DZA65_RS10990 at 2325.156 kb on - strand, within DZA65_RS10990 at 2325.156 kb on - strand, within DZA65_RS10990 at 2325.246 kb on - strand, within DZA65_RS10990 at 2325.255 kb on - strand, within DZA65_RS10990 at 2325.309 kb on + strand at 2325.310 kb on - strand at 2325.341 kb on + strand at 2325.418 kb on + strand at 2325.419 kb on - strand at 2325.433 kb on - strand at 2325.502 kb on + strand at 2325.502 kb on + strand at 2325.503 kb on - strand at 2325.503 kb on - strand at 2325.503 kb on - strand at 2325.503 kb on - strand at 2325.503 kb on - strand at 2325.506 kb on + strand at 2325.536 kb on - strand at 2325.536 kb on - strand at 2325.536 kb on - strand at 2325.545 kb on + strand at 2325.545 kb on + strand at 2325.546 kb on - strand at 2325.546 kb on - strand at 2325.575 kb on - strand at 2325.600 kb on + strand at 2325.600 kb on + strand at 2325.600 kb on + strand at 2325.600 kb on + strand at 2325.601 kb on - strand at 2325.601 kb on - strand at 2325.601 kb on - strand at 2325.601 kb on - strand at 2325.638 kb on + strand at 2325.649 kb on + strand at 2325.657 kb on - strand at 2325.720 kb on + strand at 2325.732 kb on + strand at 2325.733 kb on - strand at 2325.766 kb on - strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.937 kb on + strand, within DZA65_RS10995 at 2325.938 kb on - strand, within DZA65_RS10995 at 2325.938 kb on - strand, within DZA65_RS10995 at 2325.938 kb on - strand, within DZA65_RS10995 at 2325.938 kb on - strand, within DZA65_RS10995 at 2325.938 kb on - strand, within DZA65_RS10995 at 2326.168 kb on + strand, within DZA65_RS10995 at 2326.169 kb on - strand, within DZA65_RS10995 at 2326.170 kb on + strand, within DZA65_RS10995 at 2326.170 kb on + strand, within DZA65_RS10995 at 2326.170 kb on + strand, within DZA65_RS10995 at 2326.170 kb on + strand, within DZA65_RS10995 at 2326.170 kb on + strand, within DZA65_RS10995 at 2326.171 kb on - strand, within DZA65_RS10995 at 2326.171 kb on - strand, within DZA65_RS10995 at 2326.171 kb on - strand, within DZA65_RS10995 at 2326.171 kb on - strand, within DZA65_RS10995 at 2326.171 kb on - strand, within DZA65_RS10995 at 2326.281 kb on + strand, within DZA65_RS10995 at 2326.281 kb on + strand at 2326.282 kb on - strand, within DZA65_RS10995 at 2326.282 kb on - strand, within DZA65_RS10995 at 2326.282 kb on - strand, within DZA65_RS10995 at 2326.299 kb on + strand, within DZA65_RS10995 at 2326.299 kb on + strand, within DZA65_RS10995 at 2326.300 kb on - strand, within DZA65_RS10995 at 2326.300 kb on - strand, within DZA65_RS10995 at 2326.312 kb on + strand, within DZA65_RS10995 at 2326.312 kb on + strand, within DZA65_RS10995 at 2326.312 kb on + strand, within DZA65_RS10995 at 2326.313 kb on - strand, within DZA65_RS10995 at 2326.313 kb on - strand, within DZA65_RS10995 at 2326.313 kb on - strand, within DZA65_RS10995 at 2326.320 kb on + strand, within DZA65_RS10995 at 2326.321 kb on - strand, within DZA65_RS10995
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 2,323,724 - DZA65_RS10980 0.80 +1.3 2,323,898 - -1.5 2,323,900 + -1.7 2,323,901 - -2.0 2,323,901 - +0.8 2,323,908 + -0.1 2,323,908 + +0.8 2,323,909 - -0.2 2,323,909 - +0.5 2,323,909 - -2.0 2,323,909 - -0.4 2,323,977 + -0.2 2,324,147 + DZA65_RS10985 0.31 +0.4 2,324,147 + DZA65_RS10985 0.31 +1.0 2,324,148 - DZA65_RS10985 0.31 -0.5 2,324,148 - DZA65_RS10985 0.31 +0.8 2,324,148 - DZA65_RS10985 0.31 +0.8 2,324,154 - DZA65_RS10985 0.32 -2.2 2,324,154 - DZA65_RS10985 0.32 -0.6 2,324,154 - DZA65_RS10985 0.32 +0.6 2,324,154 - DZA65_RS10985 0.32 -1.4 2,324,165 + DZA65_RS10985 0.34 -0.5 2,324,165 + DZA65_RS10985 0.34 -0.5 2,324,165 + DZA65_RS10985 0.34 +0.8 2,324,165 + DZA65_RS10985 0.34 +0.1 2,324,165 + DZA65_RS10985 0.34 -2.2 2,324,165 + DZA65_RS10985 0.34 -0.2 2,324,166 - DZA65_RS10985 0.34 +1.0 2,324,166 - DZA65_RS10985 0.34 +1.1 2,324,166 - DZA65_RS10985 0.34 +1.8 2,324,166 - DZA65_RS10985 0.34 +0.4 2,324,166 - DZA65_RS10985 0.34 -0.2 2,324,166 - DZA65_RS10985 0.34 -1.2 2,324,166 - DZA65_RS10985 0.34 -1.3 2,324,166 - DZA65_RS10985 0.34 +1.4 2,324,166 - DZA65_RS10985 0.34 -1.4 2,324,461 + DZA65_RS10985 0.84 -0.8 2,324,461 + DZA65_RS10985 0.84 -0.8 2,324,462 - DZA65_RS10985 0.84 -0.4 2,324,462 - DZA65_RS10985 0.84 +0.5 2,324,657 - DZA65_RS10990 0.10 -2.3 2,324,657 - DZA65_RS10990 0.10 -1.1 2,324,661 - DZA65_RS10990 0.11 +1.8 2,324,661 - DZA65_RS10990 0.11 +1.0 2,324,666 + DZA65_RS10990 0.11 -1.7 2,324,666 + DZA65_RS10990 0.11 -0.4 2,324,666 + DZA65_RS10990 0.11 +0.2 2,324,666 + DZA65_RS10990 0.11 -0.2 2,324,770 - DZA65_RS10990 0.25 +3.4 2,324,770 - DZA65_RS10990 0.25 +2.4 2,324,953 - DZA65_RS10990 0.49 +1.5 2,324,953 - DZA65_RS10990 0.49 -0.4 2,324,953 - DZA65_RS10990 0.49 -0.2 2,324,953 - DZA65_RS10990 0.49 -0.2 2,325,054 + DZA65_RS10990 0.62 +0.3 2,325,054 + DZA65_RS10990 0.62 -0.6 2,325,054 + DZA65_RS10990 0.62 -0.0 2,325,054 + DZA65_RS10990 0.62 -0.8 2,325,055 - DZA65_RS10990 0.62 +1.6 2,325,055 - DZA65_RS10990 0.62 -2.5 2,325,138 + DZA65_RS10990 0.73 -3.0 2,325,138 + DZA65_RS10990 0.73 -0.6 2,325,138 + DZA65_RS10990 0.73 -1.0 2,325,155 + DZA65_RS10990 0.75 +1.0 2,325,156 - DZA65_RS10990 0.75 -0.9 2,325,156 - DZA65_RS10990 0.75 -0.5 2,325,156 - DZA65_RS10990 0.75 +0.2 2,325,246 - DZA65_RS10990 0.87 +2.2 2,325,255 - DZA65_RS10990 0.88 -0.9 2,325,309 + +0.2 2,325,310 - -1.1 2,325,341 + -2.0 2,325,418 + -1.4 2,325,419 - -0.5 2,325,433 - -2.0 2,325,502 + -1.1 2,325,502 + -0.0 2,325,503 - -1.0 2,325,503 - -2.2 2,325,503 - +0.8 2,325,503 - +1.8 2,325,503 - -0.8 2,325,506 + +0.1 2,325,536 - -1.5 2,325,536 - -1.3 2,325,536 - -0.2 2,325,545 + -2.2 2,325,545 + +0.6 2,325,546 - +1.6 2,325,546 - -1.8 2,325,575 - +1.5 2,325,600 + -1.4 2,325,600 + -0.2 2,325,600 + +0.8 2,325,600 + -0.8 2,325,601 - -1.2 2,325,601 - +1.1 2,325,601 - +0.2 2,325,601 - +0.8 2,325,638 + -0.3 2,325,649 + -0.1 2,325,657 - +0.0 2,325,720 + +0.6 2,325,732 + +0.8 2,325,733 - +0.5 2,325,766 - DZA65_RS10995 0.13 +1.4 2,325,937 + DZA65_RS10995 0.29 +0.0 2,325,937 + DZA65_RS10995 0.29 -0.9 2,325,937 + DZA65_RS10995 0.29 +0.4 2,325,937 + DZA65_RS10995 0.29 -0.5 2,325,937 + DZA65_RS10995 0.29 +0.2 2,325,937 + DZA65_RS10995 0.29 -1.5 2,325,937 + DZA65_RS10995 0.29 +1.4 2,325,937 + DZA65_RS10995 0.29 -0.4 2,325,937 + DZA65_RS10995 0.29 +1.2 2,325,937 + DZA65_RS10995 0.29 +0.4 2,325,937 + DZA65_RS10995 0.29 -1.0 2,325,938 - DZA65_RS10995 0.29 -2.2 2,325,938 - DZA65_RS10995 0.29 -0.0 2,325,938 - DZA65_RS10995 0.29 -0.2 2,325,938 - DZA65_RS10995 0.29 -1.7 2,325,938 - DZA65_RS10995 0.29 +0.0 2,326,168 + DZA65_RS10995 0.51 -1.6 2,326,169 - DZA65_RS10995 0.51 -1.0 2,326,170 + DZA65_RS10995 0.51 -0.5 2,326,170 + DZA65_RS10995 0.51 +0.2 2,326,170 + DZA65_RS10995 0.51 -0.2 2,326,170 + DZA65_RS10995 0.51 -0.2 2,326,170 + DZA65_RS10995 0.51 -1.0 2,326,171 - DZA65_RS10995 0.52 -1.4 2,326,171 - DZA65_RS10995 0.52 -0.2 2,326,171 - DZA65_RS10995 0.52 -0.2 2,326,171 - DZA65_RS10995 0.52 -1.4 2,326,171 - DZA65_RS10995 0.52 -1.2 2,326,281 + DZA65_RS10995 0.62 -0.6 2,326,281 + +0.6 2,326,282 - DZA65_RS10995 0.62 +0.9 2,326,282 - DZA65_RS10995 0.62 +0.0 2,326,282 - DZA65_RS10995 0.62 -1.1 2,326,299 + DZA65_RS10995 0.64 -0.1 2,326,299 + DZA65_RS10995 0.64 -1.8 2,326,300 - DZA65_RS10995 0.64 -1.5 2,326,300 - DZA65_RS10995 0.64 -0.9 2,326,312 + DZA65_RS10995 0.65 -2.7 2,326,312 + DZA65_RS10995 0.65 +0.8 2,326,312 + DZA65_RS10995 0.65 -0.4 2,326,313 - DZA65_RS10995 0.65 +0.1 2,326,313 - DZA65_RS10995 0.65 +1.2 2,326,313 - DZA65_RS10995 0.65 +0.8 2,326,320 + DZA65_RS10995 0.66 -0.4 2,326,321 - DZA65_RS10995 0.66 +0.8
Or see this region's nucleotide sequence