Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10475 and DZA65_RS10480 overlap by 4 nucleotides DZA65_RS10480 and DZA65_RS10485 are separated by 122 nucleotides DZA65_RS10485 and DZA65_RS10490 are separated by 169 nucleotides
DZA65_RS10475: DZA65_RS10475 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 2,223,736 to 2,224,707
_RS10475
DZA65_RS10480: DZA65_RS10480 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 2,224,704 to 2,225,447
_RS10480
DZA65_RS10485: DZA65_RS10485 - membrane protein, at 2,225,570 to 2,225,965
_RS10485
DZA65_RS10490: DZA65_RS10490 - DUF72 domain-containing protein, at 2,226,135 to 2,226,947
_RS10490
Position (kb)
2224
2225
2226 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2223.889 kb on + strand, within DZA65_RS10475 at 2223.889 kb on + strand, within DZA65_RS10475 at 2223.960 kb on - strand, within DZA65_RS10475 at 2224.109 kb on + strand at 2224.110 kb on - strand, within DZA65_RS10475 at 2224.110 kb on - strand, within DZA65_RS10475 at 2224.110 kb on - strand, within DZA65_RS10475 at 2224.110 kb on - strand, within DZA65_RS10475 at 2224.216 kb on + strand at 2224.576 kb on - strand, within DZA65_RS10475 at 2224.603 kb on + strand, within DZA65_RS10475 at 2224.604 kb on - strand, within DZA65_RS10475 at 2224.644 kb on + strand at 2224.644 kb on + strand at 2224.644 kb on + strand at 2224.644 kb on + strand at 2224.645 kb on - strand at 2224.647 kb on + strand at 2224.648 kb on - strand at 2224.648 kb on - strand at 2224.686 kb on - strand at 2224.748 kb on + strand at 2224.749 kb on - strand at 2224.749 kb on - strand at 2225.007 kb on + strand, within DZA65_RS10480 at 2225.007 kb on + strand, within DZA65_RS10480 at 2225.007 kb on + strand, within DZA65_RS10480 at 2225.008 kb on - strand, within DZA65_RS10480 at 2225.082 kb on - strand, within DZA65_RS10480 at 2225.267 kb on - strand, within DZA65_RS10480 at 2225.306 kb on + strand, within DZA65_RS10480 at 2225.307 kb on - strand, within DZA65_RS10480 at 2225.321 kb on + strand, within DZA65_RS10480 at 2225.322 kb on - strand, within DZA65_RS10480 at 2225.322 kb on - strand, within DZA65_RS10480 at 2225.432 kb on + strand at 2225.447 kb on + strand at 2225.448 kb on - strand at 2225.448 kb on - strand at 2225.448 kb on - strand at 2225.448 kb on - strand at 2225.448 kb on - strand at 2225.463 kb on + strand at 2225.463 kb on + strand at 2225.463 kb on + strand at 2225.463 kb on + strand at 2225.463 kb on + strand at 2225.464 kb on - strand at 2225.488 kb on + strand at 2225.489 kb on - strand at 2225.513 kb on - strand at 2225.549 kb on + strand at 2225.558 kb on + strand at 2225.572 kb on - strand at 2225.581 kb on + strand at 2225.585 kb on + strand at 2225.604 kb on + strand at 2225.605 kb on - strand at 2225.607 kb on + strand at 2225.607 kb on + strand at 2225.608 kb on - strand at 2225.608 kb on - strand at 2225.643 kb on + strand, within DZA65_RS10485 at 2225.643 kb on + strand, within DZA65_RS10485 at 2225.643 kb on + strand at 2225.643 kb on + strand, within DZA65_RS10485 at 2225.644 kb on - strand, within DZA65_RS10485 at 2225.644 kb on - strand, within DZA65_RS10485 at 2225.644 kb on - strand, within DZA65_RS10485 at 2225.644 kb on - strand, within DZA65_RS10485 at 2225.644 kb on - strand, within DZA65_RS10485 at 2225.644 kb on - strand, within DZA65_RS10485 at 2225.648 kb on - strand, within DZA65_RS10485 at 2225.697 kb on + strand, within DZA65_RS10485 at 2225.698 kb on - strand, within DZA65_RS10485 at 2225.701 kb on - strand at 2225.701 kb on - strand, within DZA65_RS10485 at 2225.808 kb on + strand, within DZA65_RS10485 at 2225.808 kb on + strand, within DZA65_RS10485 at 2225.809 kb on - strand, within DZA65_RS10485 at 2225.924 kb on + strand, within DZA65_RS10485 at 2225.924 kb on + strand, within DZA65_RS10485 at 2225.950 kb on - strand at 2225.950 kb on - strand at 2226.003 kb on - strand at 2226.007 kb on + strand at 2226.008 kb on - strand at 2226.008 kb on - strand at 2226.023 kb on + strand at 2226.027 kb on + strand at 2226.027 kb on + strand at 2226.027 kb on + strand at 2226.028 kb on - strand at 2226.028 kb on - strand at 2226.028 kb on - strand at 2226.028 kb on - strand at 2226.028 kb on - strand at 2226.038 kb on + strand at 2226.039 kb on - strand at 2226.039 kb on - strand at 2226.039 kb on - strand at 2226.044 kb on - strand at 2226.044 kb on - strand at 2226.044 kb on - strand at 2226.071 kb on - strand at 2226.071 kb on - strand at 2226.073 kb on + strand at 2226.073 kb on + strand at 2226.074 kb on - strand at 2226.252 kb on - strand, within DZA65_RS10490 at 2226.257 kb on + strand, within DZA65_RS10490 at 2226.258 kb on - strand, within DZA65_RS10490 at 2226.266 kb on - strand, within DZA65_RS10490 at 2226.266 kb on - strand, within DZA65_RS10490 at 2226.441 kb on - strand, within DZA65_RS10490
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 2,223,889 + DZA65_RS10475 0.16 -0.4 2,223,889 + DZA65_RS10475 0.16 +1.4 2,223,960 - DZA65_RS10475 0.23 +0.5 2,224,109 + -1.2 2,224,110 - DZA65_RS10475 0.38 +0.8 2,224,110 - DZA65_RS10475 0.38 +0.2 2,224,110 - DZA65_RS10475 0.38 -1.5 2,224,110 - DZA65_RS10475 0.38 -1.2 2,224,216 + +0.6 2,224,576 - DZA65_RS10475 0.86 -1.0 2,224,603 + DZA65_RS10475 0.89 -0.2 2,224,604 - DZA65_RS10475 0.89 +0.8 2,224,644 + -0.3 2,224,644 + -0.0 2,224,644 + -0.7 2,224,644 + -2.4 2,224,645 - -1.2 2,224,647 + +0.8 2,224,648 - +0.2 2,224,648 - -0.5 2,224,686 - -0.2 2,224,748 + +0.5 2,224,749 - +0.2 2,224,749 - +0.8 2,225,007 + DZA65_RS10480 0.41 -0.2 2,225,007 + DZA65_RS10480 0.41 -1.2 2,225,007 + DZA65_RS10480 0.41 -0.8 2,225,008 - DZA65_RS10480 0.41 -0.8 2,225,082 - DZA65_RS10480 0.51 -0.8 2,225,267 - DZA65_RS10480 0.76 +0.8 2,225,306 + DZA65_RS10480 0.81 +0.8 2,225,307 - DZA65_RS10480 0.81 -1.5 2,225,321 + DZA65_RS10480 0.83 +0.1 2,225,322 - DZA65_RS10480 0.83 -1.5 2,225,322 - DZA65_RS10480 0.83 -2.2 2,225,432 + +0.8 2,225,447 + -1.2 2,225,448 - -2.6 2,225,448 - +1.4 2,225,448 - -0.5 2,225,448 - -1.8 2,225,448 - -0.8 2,225,463 + -0.0 2,225,463 + +0.6 2,225,463 + -0.4 2,225,463 + +1.4 2,225,463 + -1.3 2,225,464 - -2.0 2,225,488 + -2.0 2,225,489 - -0.2 2,225,513 - -1.1 2,225,549 + -1.8 2,225,558 + -1.2 2,225,572 - -0.2 2,225,581 + +1.2 2,225,585 + -0.5 2,225,604 + -2.5 2,225,605 - -2.0 2,225,607 + -0.8 2,225,607 + -0.8 2,225,608 - +0.5 2,225,608 - +0.8 2,225,643 + DZA65_RS10485 0.18 -0.2 2,225,643 + DZA65_RS10485 0.18 -0.0 2,225,643 + -1.8 2,225,643 + DZA65_RS10485 0.18 +0.2 2,225,644 - DZA65_RS10485 0.19 -0.8 2,225,644 - DZA65_RS10485 0.19 -0.8 2,225,644 - DZA65_RS10485 0.19 -0.2 2,225,644 - DZA65_RS10485 0.19 -2.5 2,225,644 - DZA65_RS10485 0.19 -1.0 2,225,644 - DZA65_RS10485 0.19 +1.3 2,225,648 - DZA65_RS10485 0.20 -1.0 2,225,697 + DZA65_RS10485 0.32 -1.9 2,225,698 - DZA65_RS10485 0.32 +0.3 2,225,701 - -2.0 2,225,701 - DZA65_RS10485 0.33 -1.2 2,225,808 + DZA65_RS10485 0.60 -2.0 2,225,808 + DZA65_RS10485 0.60 -3.4 2,225,809 - DZA65_RS10485 0.60 -1.2 2,225,924 + DZA65_RS10485 0.89 -1.0 2,225,924 + DZA65_RS10485 0.89 -0.5 2,225,950 - -2.5 2,225,950 - -2.7 2,226,003 - -1.0 2,226,007 + -2.0 2,226,008 - -0.3 2,226,008 - -1.3 2,226,023 + -0.4 2,226,027 + -1.0 2,226,027 + -0.8 2,226,027 + -0.7 2,226,028 - -0.2 2,226,028 - +0.6 2,226,028 - +0.0 2,226,028 - -0.5 2,226,028 - -1.0 2,226,038 + +0.6 2,226,039 - -0.2 2,226,039 - +0.4 2,226,039 - +0.8 2,226,044 - -0.5 2,226,044 - -0.5 2,226,044 - -0.6 2,226,071 - -0.8 2,226,071 - -1.5 2,226,073 + -1.5 2,226,073 + -0.9 2,226,074 - -1.2 2,226,252 - DZA65_RS10490 0.14 +0.2 2,226,257 + DZA65_RS10490 0.15 -1.6 2,226,258 - DZA65_RS10490 0.15 +0.8 2,226,266 - DZA65_RS10490 0.16 -2.8 2,226,266 - DZA65_RS10490 0.16 -0.3 2,226,441 - DZA65_RS10490 0.38 -1.8
Or see this region's nucleotide sequence