Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_27380 and NIAGMN_27385 are separated by 50 nucleotides NIAGMN_27385 and NIAGMN_27390 are separated by 4 nucleotides NIAGMN_27390 and NIAGMN_27395 are separated by 149 nucleotides NIAGMN_27395 and Arg_trna are separated by 523 nucleotides Arg_trna and Ile2_trna are separated by 80 nucleotides Ile2_trna and yhdJ are separated by 40 nucleotides
NIAGMN_27380: NIAGMN_27380 - lysozyme, at 5,315,941 to 5,316,474
_27380
NIAGMN_27385: NIAGMN_27385 - DUF1327 domain-containing protein, at 5,316,525 to 5,316,869
_27385
NIAGMN_27390: NIAGMN_27390 - holin, at 5,316,874 to 5,317,089
_27390
NIAGMN_27395: NIAGMN_27395 - DUF1737 domain-containing protein, at 5,317,239 to 5,319,092
_27395
NIAGMN_27410: Arg_trna - tRNA-Arg(tct), at 5,319,616 to 5,319,692
_trna
NIAGMN_27415: Ile2_trna - tRNA-Ile2(cat), at 5,319,773 to 5,319,848
_trna
NIAGMN_27420: yhdJ - DNA adenine methylase, at 5,319,889 to 5,320,947
yhdJ
Position (kb) 5317
5318
5319
5320 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5319.304 kb on - strand at 5319.334 kb on - strand at 5319.336 kb on - strand at 5319.352 kb on + strand at 5319.418 kb on - strand at 5319.420 kb on - strand at 5319.605 kb on + strand at 5319.840 kb on + strand, within Ile2_trna at 5319.860 kb on - strand at 5319.863 kb on - strand at 5319.876 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 5,319,304 - +1.3 5,319,334 - -1.8 5,319,336 - -0.8 5,319,352 + +0.6 5,319,418 - -3.7 5,319,420 - -0.2 5,319,605 + -1.9 5,319,840 + Ile2_trna NIAGMN_27415 0.88 +0.1 5,319,860 - +0.8 5,319,863 - +0.0 5,319,876 + +0.1
Or see this region's nucleotide sequence