Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjP and ugpA overlap by 14 nucleotides ugpA and NIAGMN_26540 are separated by 20 nucleotides NIAGMN_26540 and ycjN are separated by 48 nucleotides ycjN and ycjM are separated by 13 nucleotides
NIAGMN_26530: ycjP - Inner membrane ABC transporter permease protein YcjP, at 5,163,764 to 5,164,606
ycjP
NIAGMN_26535: ugpA - sugar ABC transporter permease, at 5,164,593 to 5,165,474
ugpA
NIAGMN_26540: NIAGMN_26540 - ABC transporter substrate-binding protein, at 5,165,495 to 5,166,109
_26540
NIAGMN_26545: ycjN - Putative ABC transporter periplasmic-binding protein YcjN, at 5,166,158 to 5,166,787
ycjN
NIAGMN_26550: ycjM - Glucosylglycerate phosphorylase, at 5,166,801 to 5,168,105
ycjM
Position (kb) 5165
5166
5167 Strain fitness (log2 ratio) -1
0
1 at 5165.114 kb on + strand, within ugpA at 5165.240 kb on - strand, within ugpA at 5165.296 kb on + strand, within ugpA at 5165.984 kb on - strand, within NIAGMN_26540 at 5166.052 kb on + strand at 5166.160 kb on - strand at 5166.571 kb on + strand, within ycjN
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 5,165,114 + ugpA NIAGMN_26535 0.59 +0.8 5,165,240 - ugpA NIAGMN_26535 0.73 +0.2 5,165,296 + ugpA NIAGMN_26535 0.80 +1.6 5,165,984 - NIAGMN_26540 0.80 +0.2 5,166,052 + -0.6 5,166,160 - +0.6 5,166,571 + ycjN NIAGMN_26545 0.66 +0.6
Or see this region's nucleotide sequence