Strain Fitness in Escherichia coli ECRC102 around NIAGMN_25930

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynbE and ldhA are separated by 207 nucleotidesldhA and hslJ are separated by 110 nucleotideshslJ and ydbJ overlap by 4 nucleotides NIAGMN_25925: ynbE - YdbH family protein, at 5,062,709 to 5,065,348 ynbE NIAGMN_25930: ldhA - D-lactate dehydrogenase, at 5,065,556 to 5,066,545 ldhA NIAGMN_25935: hslJ - heat shock protein HslJ, at 5,066,656 to 5,067,078 hslJ NIAGMN_25940: ydbJ - Uncharacterized protein YdbJ, at 5,067,075 to 5,067,341 ydbJ Position (kb) 5065 5066 5067Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5064.763 kb on + strand, within ynbEat 5064.763 kb on + strand, within ynbEat 5065.074 kb on - strand, within ynbEat 5065.310 kb on + strandat 5065.356 kb on + strandat 5065.436 kb on + strandat 5065.441 kb on + strandat 5065.442 kb on - strandat 5065.447 kb on + strandat 5065.447 kb on + strandat 5065.447 kb on + strandat 5065.447 kb on + strandat 5065.448 kb on - strandat 5065.448 kb on - strandat 5065.702 kb on + strand, within ldhAat 5065.768 kb on + strand, within ldhAat 5065.978 kb on + strand, within ldhAat 5065.978 kb on + strand, within ldhAat 5065.982 kb on + strand, within ldhAat 5066.118 kb on - strand, within ldhAat 5066.248 kb on + strand, within ldhAat 5066.499 kb on + strandat 5066.708 kb on + strand, within hslJat 5067.047 kb on + strandat 5067.048 kb on - strandat 5067.055 kb on - strandat 5067.055 kb on - strandat 5067.385 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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5,064,763 + ynbE NIAGMN_25925 0.78 +0.3
5,064,763 + ynbE NIAGMN_25925 0.78 -0.4
5,065,074 - ynbE NIAGMN_25925 0.90 -0.2
5,065,310 + +0.2
5,065,356 + -1.3
5,065,436 + -1.3
5,065,441 + +0.3
5,065,442 - -0.4
5,065,447 + -0.3
5,065,447 + -0.9
5,065,447 + +0.4
5,065,447 + +0.5
5,065,448 - +0.4
5,065,448 - -0.9
5,065,702 + ldhA NIAGMN_25930 0.15 -0.1
5,065,768 + ldhA NIAGMN_25930 0.21 +0.8
5,065,978 + ldhA NIAGMN_25930 0.43 -3.1
5,065,978 + ldhA NIAGMN_25930 0.43 +1.5
5,065,982 + ldhA NIAGMN_25930 0.43 +0.3
5,066,118 - ldhA NIAGMN_25930 0.57 -1.0
5,066,248 + ldhA NIAGMN_25930 0.70 +0.9
5,066,499 + -1.0
5,066,708 + hslJ NIAGMN_25935 0.12 -1.0
5,067,047 + -2.8
5,067,048 - +1.8
5,067,055 - +0.6
5,067,055 - -1.3
5,067,385 + +0.4

Or see this region's nucleotide sequence