Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt curA and mddA are separated by 180 nucleotides mddA and NIAGMN_25675 overlap by 4 nucleotides NIAGMN_25675 and ydcY are separated by 0 nucleotides ydcY and ortT are separated by 85 nucleotides
NIAGMN_25665: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 5,010,074 to 5,011,111
curA
NIAGMN_25670: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 5,011,292 to 5,011,810
mddA
NIAGMN_25675: NIAGMN_25675 - Putative inner membrane exporter, YdcZ, at 5,011,807 to 5,012,256
_25675
NIAGMN_25680: ydcY - Uncharacterized protein YdcY, at 5,012,257 to 5,012,490
ydcY
NIAGMN_25685: ortT - orphan toxin OrtT, at 5,012,576 to 5,012,749
ortT
Position (kb) 5011
5012 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5010.395 kb on - strand, within curA at 5010.397 kb on + strand, within curA at 5010.697 kb on + strand, within curA at 5010.791 kb on + strand, within curA at 5010.792 kb on - strand, within curA at 5011.344 kb on - strand, within mddA at 5011.362 kb on - strand, within mddA at 5011.474 kb on - strand, within mddA at 5011.960 kb on - strand, within NIAGMN_25675 at 5012.104 kb on - strand, within NIAGMN_25675 at 5012.255 kb on - strand at 5012.490 kb on + strand at 5012.540 kb on - strand at 5012.751 kb on + strand at 5012.751 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 5,010,395 - curA NIAGMN_25665 0.31 +0.6 5,010,397 + curA NIAGMN_25665 0.31 -0.6 5,010,697 + curA NIAGMN_25665 0.60 -0.0 5,010,791 + curA NIAGMN_25665 0.69 +2.2 5,010,792 - curA NIAGMN_25665 0.69 +0.6 5,011,344 - mddA NIAGMN_25670 0.10 -0.0 5,011,362 - mddA NIAGMN_25670 0.13 -0.0 5,011,474 - mddA NIAGMN_25670 0.35 +0.2 5,011,960 - NIAGMN_25675 0.34 +1.0 5,012,104 - NIAGMN_25675 0.66 +0.7 5,012,255 - -0.5 5,012,490 + +0.4 5,012,540 - +0.5 5,012,751 + -2.0 5,012,751 + -0.7
Or see this region's nucleotide sequence