Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kilAC and ninG overlap by 1 nucleotides ninG and ninH overlap by 4 nucleotides ninH and NIAGMN_23420 overlap by 8 nucleotides NIAGMN_23420 and Ile2_trna are separated by 441 nucleotides Ile2_trna and Arg_trna are separated by 9 nucleotides Arg_trna and Arg_trna are separated by 13 nucleotides Arg_trna and stxA2c are separated by 90 nucleotides
NIAGMN_23405: kilAC - DNA-binding protein, at 4,585,541 to 4,586,263
kilAC
NIAGMN_23410: ninG - Protein ninG, at 4,586,263 to 4,586,868
ninG
NIAGMN_23415: ninH - Protein ninH, at 4,586,865 to 4,587,059
ninH
NIAGMN_23420: NIAGMN_23420 - Antitermination protein Q, at 4,587,052 to 4,587,486
_23420
NIAGMN_23425: Ile2_trna - tRNA-Ile2(cat), at 4,587,928 to 4,588,003
_trna
NIAGMN_23430: Arg_trna - tRNA-Arg(tcg), at 4,588,013 to 4,588,089
_trna
NIAGMN_23435: Arg_trna - tRNA-Arg(tct), at 4,588,103 to 4,588,179
_trna
NIAGMN_23440: stxA2c - Shiga toxin Stx2c subunit A, at 4,588,270 to 4,589,229
stxA2c
Position (kb) 4587
4588 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4586.063 kb on - strand, within kilAC at 4586.147 kb on - strand, within kilAC at 4587.020 kb on + strand, within ninH at 4587.102 kb on + strand, within NIAGMN_23420 at 4587.102 kb on + strand, within NIAGMN_23420 at 4587.106 kb on + strand, within NIAGMN_23420 at 4587.117 kb on + strand, within NIAGMN_23420 at 4587.117 kb on + strand, within NIAGMN_23420 at 4587.117 kb on + strand, within NIAGMN_23420 at 4587.117 kb on + strand, within NIAGMN_23420 at 4587.118 kb on - strand, within NIAGMN_23420 at 4587.209 kb on + strand, within NIAGMN_23420 at 4587.271 kb on + strand, within NIAGMN_23420 at 4587.274 kb on + strand, within NIAGMN_23420 at 4587.643 kb on + strand at 4587.643 kb on + strand at 4587.643 kb on + strand at 4587.664 kb on + strand at 4587.669 kb on + strand at 4587.869 kb on - strand at 4588.004 kb on + strand at 4588.004 kb on + strand at 4588.004 kb on + strand at 4588.092 kb on - strand at 4588.101 kb on - strand at 4588.101 kb on - strand at 4588.180 kb on + strand at 4588.372 kb on + strand, within stxA2c at 4588.395 kb on - strand, within stxA2c at 4588.427 kb on + strand, within stxA2c
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,586,063 - kilAC NIAGMN_23405 0.72 +0.3 4,586,147 - kilAC NIAGMN_23405 0.84 -2.3 4,587,020 + ninH NIAGMN_23415 0.79 -0.3 4,587,102 + NIAGMN_23420 0.11 +0.1 4,587,102 + NIAGMN_23420 0.11 -2.4 4,587,106 + NIAGMN_23420 0.12 +1.4 4,587,117 + NIAGMN_23420 0.15 -2.3 4,587,117 + NIAGMN_23420 0.15 +1.6 4,587,117 + NIAGMN_23420 0.15 +0.8 4,587,117 + NIAGMN_23420 0.15 -0.9 4,587,118 - NIAGMN_23420 0.15 -0.7 4,587,209 + NIAGMN_23420 0.36 -0.8 4,587,271 + NIAGMN_23420 0.50 -0.1 4,587,274 + NIAGMN_23420 0.51 -0.1 4,587,643 + +0.5 4,587,643 + +0.1 4,587,643 + -0.1 4,587,664 + -1.6 4,587,669 + +0.4 4,587,869 - -0.1 4,588,004 + -1.1 4,588,004 + -0.8 4,588,004 + -0.6 4,588,092 - -0.2 4,588,101 - -1.8 4,588,101 - -0.1 4,588,180 + +0.2 4,588,372 + stxA2c NIAGMN_23440 0.11 -2.9 4,588,395 - stxA2c NIAGMN_23440 0.13 +0.0 4,588,427 + stxA2c NIAGMN_23440 0.16 +0.2
Or see this region's nucleotide sequence