Strain Fitness in Escherichia coli ECRC102 around NIAGMN_23040

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_23030 and Ser_trna are separated by 104 nucleotidesSer_trna and hyaA are separated by 425 nucleotideshyaA and hyaB overlap by 4 nucleotides NIAGMN_23030: NIAGMN_23030 - hypothetical protein, at 4,531,764 to 4,531,946 _23030 NIAGMN_23035: Ser_trna - tRNA-Ser(tga), at 4,532,051 to 4,532,138 _trna NIAGMN_23040: hyaA - hydrogenase 1 small subunit, at 4,532,564 to 4,533,682 hyaA NIAGMN_23045: hyaB - Ni/Fe-hydrogenase large subunit, at 4,533,679 to 4,535,472 hyaB Position (kb) 4532 4533 4534Strain fitness (log2 ratio) -2 -1 0 1at 4531.610 kb on - strandat 4531.664 kb on - strandat 4531.838 kb on - strand, within NIAGMN_23030at 4532.331 kb on - strandat 4532.412 kb on + strandat 4532.413 kb on - strandat 4532.413 kb on - strandat 4532.728 kb on - strand, within hyaAat 4532.898 kb on - strand, within hyaAat 4532.909 kb on + strand, within hyaAat 4532.911 kb on + strand, within hyaAat 4532.962 kb on + strand, within hyaAat 4532.984 kb on - strand, within hyaAat 4533.178 kb on + strand, within hyaAat 4533.182 kb on + strand, within hyaAat 4533.183 kb on - strand, within hyaAat 4533.183 kb on - strand, within hyaAat 4533.371 kb on - strand, within hyaAat 4533.377 kb on + strand, within hyaAat 4533.625 kb on + strandat 4533.717 kb on + strandat 4533.787 kb on - strandat 4533.804 kb on - strandat 4533.900 kb on + strand, within hyaBat 4533.969 kb on + strand, within hyaBat 4534.175 kb on + strand, within hyaB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,531,610 - -2.0
4,531,664 - -1.1
4,531,838 - NIAGMN_23030 0.40 -0.1
4,532,331 - -1.3
4,532,412 + +0.6
4,532,413 - +1.0
4,532,413 - -1.5
4,532,728 - hyaA NIAGMN_23040 0.15 +0.5
4,532,898 - hyaA NIAGMN_23040 0.30 +0.6
4,532,909 + hyaA NIAGMN_23040 0.31 +0.3
4,532,911 + hyaA NIAGMN_23040 0.31 -0.6
4,532,962 + hyaA NIAGMN_23040 0.36 -0.4
4,532,984 - hyaA NIAGMN_23040 0.38 +0.6
4,533,178 + hyaA NIAGMN_23040 0.55 -0.1
4,533,182 + hyaA NIAGMN_23040 0.55 +1.0
4,533,183 - hyaA NIAGMN_23040 0.55 -0.9
4,533,183 - hyaA NIAGMN_23040 0.55 -1.1
4,533,371 - hyaA NIAGMN_23040 0.72 +0.3
4,533,377 + hyaA NIAGMN_23040 0.73 +0.8
4,533,625 + -1.4
4,533,717 + -1.0
4,533,787 - +0.8
4,533,804 - +0.6
4,533,900 + hyaB NIAGMN_23045 0.12 +0.8
4,533,969 + hyaB NIAGMN_23045 0.16 -0.5
4,534,175 + hyaB NIAGMN_23045 0.28 +0.6

Or see this region's nucleotide sequence