Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cbtA and yeeT are separated by 73 nucleotides yeeT and yeeS are separated by 62 nucleotides yeeS and NIAGMN_22560 are separated by 14 nucleotides NIAGMN_22560 and NIAGMN_22565 are separated by 81 nucleotides
NIAGMN_22545: cbtA - type IV toxin-antitoxin system toxin CbtA, at 4,450,385 to 4,450,753
cbtA
NIAGMN_22550: yeeT - Uncharacterized protein YeeT, at 4,450,827 to 4,451,048
yeeT
NIAGMN_22555: yeeS - UPF0758 protein YeeS, at 4,451,111 to 4,451,587
yeeS
NIAGMN_22560: NIAGMN_22560 - restriction endonuclease, at 4,451,602 to 4,452,081
_22560
NIAGMN_22565: NIAGMN_22565 - DUF932 domain-containing protein, at 4,452,163 to 4,452,984
_22565
Position (kb) 4451
4452
4453 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4450.936 kb on + strand, within yeeT at 4450.964 kb on - strand, within yeeT at 4450.964 kb on - strand, within yeeT at 4451.015 kb on + strand, within yeeT at 4451.620 kb on - strand at 4451.620 kb on - strand at 4451.718 kb on - strand, within NIAGMN_22560 at 4451.718 kb on - strand, within NIAGMN_22560 at 4452.845 kb on + strand, within NIAGMN_22565 at 4452.846 kb on - strand, within NIAGMN_22565 at 4453.024 kb on + strand at 4453.025 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,450,936 + yeeT NIAGMN_22550 0.49 +0.9 4,450,964 - yeeT NIAGMN_22550 0.62 +0.4 4,450,964 - yeeT NIAGMN_22550 0.62 +1.1 4,451,015 + yeeT NIAGMN_22550 0.85 -1.3 4,451,620 - +1.4 4,451,620 - +0.5 4,451,718 - NIAGMN_22560 0.24 +1.5 4,451,718 - NIAGMN_22560 0.24 -0.0 4,452,845 + NIAGMN_22565 0.83 -4.1 4,452,846 - NIAGMN_22565 0.83 +1.1 4,453,024 + -2.1 4,453,025 - +2.1
Or see this region's nucleotide sequence