Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzxC and wcaK are separated by 179 nucleotides wcaK and wcaL overlap by 4 nucleotides
NIAGMN_22325: wzxC - colanic acid undecaprenyl disphosphate flippase WzxC, at 4,404,930 to 4,406,408
wzxC
NIAGMN_22330: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 4,406,588 to 4,407,868
wcaK
NIAGMN_22335: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 4,407,865 to 4,409,085
wcaL
Position (kb)
4406
4407
4408 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4405.678 kb on - strand, within wzxC at 4405.857 kb on - strand, within wzxC at 4406.169 kb on + strand, within wzxC at 4406.450 kb on + strand at 4406.596 kb on - strand at 4406.596 kb on - strand at 4406.851 kb on - strand, within wcaK at 4406.928 kb on + strand, within wcaK at 4406.940 kb on - strand, within wcaK at 4407.044 kb on - strand, within wcaK at 4407.086 kb on - strand, within wcaK at 4407.172 kb on + strand, within wcaK at 4407.172 kb on + strand, within wcaK at 4407.449 kb on + strand, within wcaK at 4407.450 kb on - strand, within wcaK at 4407.520 kb on - strand, within wcaK at 4407.520 kb on - strand, within wcaK at 4407.625 kb on + strand, within wcaK at 4407.648 kb on - strand, within wcaK at 4407.648 kb on - strand, within wcaK at 4407.656 kb on - strand, within wcaK at 4407.756 kb on + strand at 4407.921 kb on + strand at 4407.939 kb on + strand at 4408.481 kb on + strand, within wcaL at 4408.481 kb on + strand, within wcaL at 4408.486 kb on - strand, within wcaL at 4408.486 kb on - strand, within wcaL at 4408.687 kb on + strand, within wcaL at 4408.803 kb on + strand, within wcaL
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,405,678 - wzxC NIAGMN_22325 0.51 +0.3 4,405,857 - wzxC NIAGMN_22325 0.63 +0.0 4,406,169 + wzxC NIAGMN_22325 0.84 +1.4 4,406,450 + +0.0 4,406,596 - -0.2 4,406,596 - +0.2 4,406,851 - wcaK NIAGMN_22330 0.21 -0.4 4,406,928 + wcaK NIAGMN_22330 0.27 -0.1 4,406,940 - wcaK NIAGMN_22330 0.27 +1.9 4,407,044 - wcaK NIAGMN_22330 0.36 +1.1 4,407,086 - wcaK NIAGMN_22330 0.39 -0.3 4,407,172 + wcaK NIAGMN_22330 0.46 +0.2 4,407,172 + wcaK NIAGMN_22330 0.46 +0.9 4,407,449 + wcaK NIAGMN_22330 0.67 +0.9 4,407,450 - wcaK NIAGMN_22330 0.67 +0.8 4,407,520 - wcaK NIAGMN_22330 0.73 -0.4 4,407,520 - wcaK NIAGMN_22330 0.73 +0.8 4,407,625 + wcaK NIAGMN_22330 0.81 -1.6 4,407,648 - wcaK NIAGMN_22330 0.83 -2.0 4,407,648 - wcaK NIAGMN_22330 0.83 -0.4 4,407,656 - wcaK NIAGMN_22330 0.83 -1.0 4,407,756 + -2.3 4,407,921 + +2.1 4,407,939 + -2.1 4,408,481 + wcaL NIAGMN_22335 0.50 -0.6 4,408,481 + wcaL NIAGMN_22335 0.50 -0.5 4,408,486 - wcaL NIAGMN_22335 0.51 +1.5 4,408,486 - wcaL NIAGMN_22335 0.51 -1.1 4,408,687 + wcaL NIAGMN_22335 0.67 +0.4 4,408,803 + wcaL NIAGMN_22335 0.77 +1.7
Or see this region's nucleotide sequence