Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaA and wcaB are separated by 2 nucleotides wcaB and wcaC overlap by 4 nucleotides wcaC and wcaD overlap by 26 nucleotides
NIAGMN_22260: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 4,391,600 to 4,392,439
wcaA
NIAGMN_22265: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 4,392,442 to 4,392,930
wcaB
NIAGMN_22270: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 4,392,927 to 4,394,144
wcaC
NIAGMN_22275: wcaD - putative colanic acid polymerase WcaD, at 4,394,119 to 4,395,336
wcaD
Position (kb)
4392
4393
4394
4395 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4391.991 kb on - strand, within wcaA at 4392.009 kb on + strand, within wcaA at 4392.442 kb on - strand at 4392.442 kb on - strand at 4392.442 kb on - strand at 4392.455 kb on + strand at 4392.652 kb on - strand, within wcaB at 4392.654 kb on + strand, within wcaB at 4392.807 kb on + strand, within wcaB at 4392.831 kb on + strand, within wcaB at 4392.925 kb on - strand at 4393.415 kb on + strand, within wcaC at 4393.415 kb on + strand, within wcaC at 4393.415 kb on + strand, within wcaC at 4393.509 kb on - strand, within wcaC at 4393.574 kb on + strand, within wcaC at 4393.684 kb on - strand, within wcaC at 4393.752 kb on - strand, within wcaC at 4393.877 kb on + strand, within wcaC at 4394.092 kb on - strand at 4394.167 kb on + strand at 4394.323 kb on - strand, within wcaD at 4394.355 kb on + strand, within wcaD at 4394.356 kb on - strand, within wcaD at 4394.356 kb on - strand, within wcaD at 4394.356 kb on - strand, within wcaD at 4394.360 kb on - strand, within wcaD at 4394.374 kb on - strand, within wcaD at 4394.463 kb on - strand, within wcaD at 4394.579 kb on - strand, within wcaD
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,391,991 - wcaA NIAGMN_22260 0.47 +0.6 4,392,009 + wcaA NIAGMN_22260 0.49 +0.2 4,392,442 - -1.4 4,392,442 - +0.3 4,392,442 - -1.1 4,392,455 + +0.3 4,392,652 - wcaB NIAGMN_22265 0.43 -1.3 4,392,654 + wcaB NIAGMN_22265 0.43 +0.4 4,392,807 + wcaB NIAGMN_22265 0.75 -0.2 4,392,831 + wcaB NIAGMN_22265 0.80 +1.1 4,392,925 - -0.0 4,393,415 + wcaC NIAGMN_22270 0.40 +0.6 4,393,415 + wcaC NIAGMN_22270 0.40 +0.4 4,393,415 + wcaC NIAGMN_22270 0.40 -1.8 4,393,509 - wcaC NIAGMN_22270 0.48 -0.8 4,393,574 + wcaC NIAGMN_22270 0.53 +0.5 4,393,684 - wcaC NIAGMN_22270 0.62 -0.1 4,393,752 - wcaC NIAGMN_22270 0.68 +0.2 4,393,877 + wcaC NIAGMN_22270 0.78 +0.3 4,394,092 - -0.8 4,394,167 + +0.9 4,394,323 - wcaD NIAGMN_22275 0.17 +1.5 4,394,355 + wcaD NIAGMN_22275 0.19 +1.4 4,394,356 - wcaD NIAGMN_22275 0.19 +1.5 4,394,356 - wcaD NIAGMN_22275 0.19 -0.6 4,394,356 - wcaD NIAGMN_22275 0.19 +0.4 4,394,360 - wcaD NIAGMN_22275 0.20 -0.7 4,394,374 - wcaD NIAGMN_22275 0.21 -1.0 4,394,463 - wcaD NIAGMN_22275 0.28 -0.8 4,394,579 - wcaD NIAGMN_22275 0.38 +2.7
Or see this region's nucleotide sequence