Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22270

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaA and wcaB are separated by 2 nucleotideswcaB and wcaC overlap by 4 nucleotideswcaC and wcaD overlap by 26 nucleotides NIAGMN_22260: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 4,391,600 to 4,392,439 wcaA NIAGMN_22265: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 4,392,442 to 4,392,930 wcaB NIAGMN_22270: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 4,392,927 to 4,394,144 wcaC NIAGMN_22275: wcaD - putative colanic acid polymerase WcaD, at 4,394,119 to 4,395,336 wcaD Position (kb) 4392 4393 4394 4395Strain fitness (log2 ratio) -2 -1 0 1 2at 4391.991 kb on - strand, within wcaAat 4392.009 kb on + strand, within wcaAat 4392.442 kb on - strandat 4392.442 kb on - strandat 4392.442 kb on - strandat 4392.455 kb on + strandat 4392.652 kb on - strand, within wcaBat 4392.654 kb on + strand, within wcaBat 4392.807 kb on + strand, within wcaBat 4392.831 kb on + strand, within wcaBat 4392.925 kb on - strandat 4393.415 kb on + strand, within wcaCat 4393.415 kb on + strand, within wcaCat 4393.415 kb on + strand, within wcaCat 4393.509 kb on - strand, within wcaCat 4393.574 kb on + strand, within wcaCat 4393.684 kb on - strand, within wcaCat 4393.752 kb on - strand, within wcaCat 4393.877 kb on + strand, within wcaCat 4394.092 kb on - strandat 4394.167 kb on + strandat 4394.323 kb on - strand, within wcaDat 4394.355 kb on + strand, within wcaDat 4394.356 kb on - strand, within wcaDat 4394.356 kb on - strand, within wcaDat 4394.356 kb on - strand, within wcaDat 4394.360 kb on - strand, within wcaDat 4394.374 kb on - strand, within wcaDat 4394.463 kb on - strand, within wcaDat 4394.579 kb on - strand, within wcaD

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,391,991 - wcaA NIAGMN_22260 0.47 +0.6
4,392,009 + wcaA NIAGMN_22260 0.49 +0.2
4,392,442 - -1.4
4,392,442 - +0.3
4,392,442 - -1.1
4,392,455 + +0.3
4,392,652 - wcaB NIAGMN_22265 0.43 -1.3
4,392,654 + wcaB NIAGMN_22265 0.43 +0.4
4,392,807 + wcaB NIAGMN_22265 0.75 -0.2
4,392,831 + wcaB NIAGMN_22265 0.80 +1.1
4,392,925 - -0.0
4,393,415 + wcaC NIAGMN_22270 0.40 +0.6
4,393,415 + wcaC NIAGMN_22270 0.40 +0.4
4,393,415 + wcaC NIAGMN_22270 0.40 -1.8
4,393,509 - wcaC NIAGMN_22270 0.48 -0.8
4,393,574 + wcaC NIAGMN_22270 0.53 +0.5
4,393,684 - wcaC NIAGMN_22270 0.62 -0.1
4,393,752 - wcaC NIAGMN_22270 0.68 +0.2
4,393,877 + wcaC NIAGMN_22270 0.78 +0.3
4,394,092 - -0.8
4,394,167 + +0.9
4,394,323 - wcaD NIAGMN_22275 0.17 +1.5
4,394,355 + wcaD NIAGMN_22275 0.19 +1.4
4,394,356 - wcaD NIAGMN_22275 0.19 +1.5
4,394,356 - wcaD NIAGMN_22275 0.19 -0.6
4,394,356 - wcaD NIAGMN_22275 0.19 +0.4
4,394,360 - wcaD NIAGMN_22275 0.20 -0.7
4,394,374 - wcaD NIAGMN_22275 0.21 -1.0
4,394,463 - wcaD NIAGMN_22275 0.28 -0.8
4,394,579 - wcaD NIAGMN_22275 0.38 +2.7

Or see this region's nucleotide sequence