Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfR and hyfJ are separated by 29 nucleotides hyfJ and hyfI overlap by 8 nucleotides hyfI and hyfH overlap by 4 nucleotides hyfH and hyfG are separated by 9 nucleotides
NIAGMN_19620: hyfR - DNA-binding transcriptional regulator HyfR, at 3,871,616 to 3,873,628
hyfR
NIAGMN_19625: hyfJ - hydrogenase 4 assembly chaperone HyfJ, at 3,873,658 to 3,874,071
hyfJ
NIAGMN_19630: hyfI - hydrogenase 4 catalytic subunit HyfI, at 3,874,064 to 3,874,822
hyfI
NIAGMN_19635: hyfH - hydrogenase 4 subunit H, at 3,874,819 to 3,875,364
hyfH
NIAGMN_19640: hyfG - hydrogenase 4 catalytic subunit HyfG, at 3,875,374 to 3,877,089
hyfG
Position (kb)
3874
3875 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3873.251 kb on - strand, within hyfR at 3873.323 kb on + strand, within hyfR at 3873.391 kb on - strand, within hyfR at 3873.391 kb on - strand, within hyfR at 3873.438 kb on + strand at 3873.470 kb on - strand at 3873.623 kb on - strand at 3873.640 kb on - strand at 3873.683 kb on + strand at 3873.684 kb on - strand at 3873.687 kb on + strand at 3873.736 kb on - strand, within hyfJ at 3873.852 kb on + strand, within hyfJ at 3873.853 kb on - strand, within hyfJ at 3873.974 kb on + strand, within hyfJ at 3873.974 kb on + strand, within hyfJ at 3873.975 kb on - strand, within hyfJ at 3873.975 kb on - strand, within hyfJ at 3873.975 kb on - strand, within hyfJ at 3873.975 kb on - strand, within hyfJ at 3874.074 kb on - strand at 3874.108 kb on + strand at 3874.108 kb on + strand at 3874.111 kb on + strand at 3874.178 kb on + strand, within hyfI at 3874.178 kb on + strand, within hyfI at 3874.178 kb on + strand, within hyfI at 3874.178 kb on + strand, within hyfI at 3874.297 kb on + strand, within hyfI at 3874.298 kb on - strand, within hyfI at 3874.298 kb on - strand, within hyfI at 3874.536 kb on + strand, within hyfI at 3874.537 kb on - strand, within hyfI at 3874.537 kb on - strand, within hyfI at 3874.555 kb on + strand, within hyfI at 3874.556 kb on - strand, within hyfI at 3874.583 kb on - strand, within hyfI at 3874.583 kb on - strand, within hyfI at 3874.583 kb on - strand, within hyfI at 3874.807 kb on + strand at 3874.808 kb on - strand at 3874.808 kb on - strand at 3874.822 kb on + strand at 3875.024 kb on - strand, within hyfH at 3875.082 kb on + strand, within hyfH at 3875.153 kb on + strand, within hyfH at 3875.197 kb on - strand, within hyfH at 3875.244 kb on + strand, within hyfH at 3875.244 kb on + strand, within hyfH at 3875.344 kb on - strand at 3875.375 kb on + strand at 3875.375 kb on + strand at 3875.426 kb on + strand at 3875.426 kb on + strand at 3875.427 kb on - strand at 3875.489 kb on + strand at 3875.552 kb on + strand, within hyfG at 3875.553 kb on - strand, within hyfG at 3875.577 kb on - strand, within hyfG at 3875.692 kb on - strand, within hyfG at 3875.693 kb on + strand, within hyfG at 3875.698 kb on - strand, within hyfG at 3875.698 kb on - strand, within hyfG at 3875.698 kb on - strand, within hyfG
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 3,873,251 - hyfR NIAGMN_19620 0.81 +0.1 3,873,323 + hyfR NIAGMN_19620 0.85 +0.8 3,873,391 - hyfR NIAGMN_19620 0.88 -0.6 3,873,391 - hyfR NIAGMN_19620 0.88 +3.6 3,873,438 + -0.9 3,873,470 - +2.2 3,873,623 - -0.1 3,873,640 - -1.1 3,873,683 + -0.4 3,873,684 - +0.2 3,873,687 + +0.6 3,873,736 - hyfJ NIAGMN_19625 0.19 +0.5 3,873,852 + hyfJ NIAGMN_19625 0.47 -0.6 3,873,853 - hyfJ NIAGMN_19625 0.47 -0.1 3,873,974 + hyfJ NIAGMN_19625 0.76 +2.3 3,873,974 + hyfJ NIAGMN_19625 0.76 +0.8 3,873,975 - hyfJ NIAGMN_19625 0.77 -0.2 3,873,975 - hyfJ NIAGMN_19625 0.77 -1.9 3,873,975 - hyfJ NIAGMN_19625 0.77 -0.9 3,873,975 - hyfJ NIAGMN_19625 0.77 -2.6 3,874,074 - -0.5 3,874,108 + -0.2 3,874,108 + +0.6 3,874,111 + +0.5 3,874,178 + hyfI NIAGMN_19630 0.15 +0.4 3,874,178 + hyfI NIAGMN_19630 0.15 -2.1 3,874,178 + hyfI NIAGMN_19630 0.15 -2.6 3,874,178 + hyfI NIAGMN_19630 0.15 -0.8 3,874,297 + hyfI NIAGMN_19630 0.31 +0.7 3,874,298 - hyfI NIAGMN_19630 0.31 +0.3 3,874,298 - hyfI NIAGMN_19630 0.31 -0.1 3,874,536 + hyfI NIAGMN_19630 0.62 -0.4 3,874,537 - hyfI NIAGMN_19630 0.62 +0.6 3,874,537 - hyfI NIAGMN_19630 0.62 +0.1 3,874,555 + hyfI NIAGMN_19630 0.65 +0.4 3,874,556 - hyfI NIAGMN_19630 0.65 +1.2 3,874,583 - hyfI NIAGMN_19630 0.68 -1.2 3,874,583 - hyfI NIAGMN_19630 0.68 +0.1 3,874,583 - hyfI NIAGMN_19630 0.68 -0.5 3,874,807 + +0.6 3,874,808 - -0.8 3,874,808 - -0.2 3,874,822 + -0.5 3,875,024 - hyfH NIAGMN_19635 0.38 -0.7 3,875,082 + hyfH NIAGMN_19635 0.48 +0.2 3,875,153 + hyfH NIAGMN_19635 0.61 +1.5 3,875,197 - hyfH NIAGMN_19635 0.69 +0.5 3,875,244 + hyfH NIAGMN_19635 0.78 +1.7 3,875,244 + hyfH NIAGMN_19635 0.78 -0.5 3,875,344 - +0.3 3,875,375 + -1.2 3,875,375 + -0.8 3,875,426 + +2.3 3,875,426 + -1.2 3,875,427 - -1.1 3,875,489 + +0.2 3,875,552 + hyfG NIAGMN_19640 0.10 -0.5 3,875,553 - hyfG NIAGMN_19640 0.10 +0.6 3,875,577 - hyfG NIAGMN_19640 0.12 -0.3 3,875,692 - hyfG NIAGMN_19640 0.19 +0.2 3,875,693 + hyfG NIAGMN_19640 0.19 +1.8 3,875,698 - hyfG NIAGMN_19640 0.19 -0.2 3,875,698 - hyfG NIAGMN_19640 0.19 +1.8 3,875,698 - hyfG NIAGMN_19640 0.19 +1.1
Or see this region's nucleotide sequence