Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt casA and casB overlap by 4 nucleotides casB and cas7e are separated by 11 nucleotides
NIAGMN_18170: casA - type I-E CRISPR-associated protein Cse1/CasA, at 3,597,376 to 3,598,938
casA
NIAGMN_18175: casB - type I-E CRISPR-associated protein Cse2/CasB, at 3,598,935 to 3,599,471
casB
NIAGMN_18180: cas7e - type I-E CRISPR-associated protein Cas7/Cse4/CasC, at 3,599,483 to 3,600,538
cas7e
Position (kb)
3598
3599
3600 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3598.060 kb on - strand, within casA at 3598.143 kb on + strand, within casA at 3598.152 kb on + strand, within casA at 3598.153 kb on - strand, within casA at 3598.179 kb on + strand, within casA at 3598.231 kb on + strand, within casA at 3598.243 kb on + strand, within casA at 3598.243 kb on + strand, within casA at 3598.243 kb on + strand, within casA at 3598.281 kb on - strand, within casA at 3598.287 kb on + strand, within casA at 3598.334 kb on + strand, within casA at 3598.335 kb on - strand, within casA at 3598.372 kb on - strand, within casA at 3598.468 kb on + strand, within casA at 3598.710 kb on - strand, within casA at 3598.748 kb on + strand, within casA at 3598.749 kb on - strand, within casA at 3598.808 kb on + strand at 3598.809 kb on - strand at 3598.822 kb on + strand at 3598.822 kb on + strand at 3598.872 kb on + strand at 3599.028 kb on - strand, within casB at 3599.030 kb on + strand, within casB at 3599.092 kb on + strand, within casB at 3599.092 kb on + strand, within casB at 3599.120 kb on + strand, within casB at 3599.120 kb on + strand, within casB at 3599.293 kb on + strand, within casB at 3599.293 kb on + strand, within casB at 3599.294 kb on - strand, within casB at 3599.406 kb on - strand, within casB at 3599.430 kb on + strand at 3599.434 kb on - strand at 3599.478 kb on - strand at 3599.494 kb on + strand at 3599.495 kb on - strand at 3599.666 kb on - strand, within cas7e at 3599.686 kb on + strand, within cas7e at 3599.707 kb on + strand, within cas7e at 3599.838 kb on + strand, within cas7e at 3599.838 kb on + strand, within cas7e at 3599.871 kb on + strand, within cas7e at 3599.958 kb on + strand, within cas7e at 3599.958 kb on + strand, within cas7e at 3599.958 kb on + strand, within cas7e at 3599.958 kb on + strand, within cas7e at 3599.959 kb on - strand, within cas7e at 3599.968 kb on + strand, within cas7e at 3599.969 kb on - strand, within cas7e at 3599.980 kb on + strand, within cas7e at 3599.980 kb on + strand, within cas7e at 3599.981 kb on - strand, within cas7e at 3599.981 kb on - strand, within cas7e at 3600.162 kb on - strand, within cas7e at 3600.162 kb on - strand, within cas7e at 3600.162 kb on - strand, within cas7e at 3600.167 kb on + strand, within cas7e at 3600.167 kb on + strand, within cas7e at 3600.167 kb on + strand, within cas7e at 3600.167 kb on + strand, within cas7e at 3600.167 kb on + strand, within cas7e at 3600.168 kb on - strand, within cas7e at 3600.168 kb on - strand, within cas7e at 3600.168 kb on - strand, within cas7e at 3600.168 kb on - strand, within cas7e at 3600.169 kb on + strand, within cas7e at 3600.169 kb on + strand, within cas7e at 3600.169 kb on + strand, within cas7e at 3600.170 kb on - strand, within cas7e at 3600.170 kb on - strand, within cas7e at 3600.222 kb on + strand, within cas7e at 3600.226 kb on + strand, within cas7e at 3600.227 kb on - strand, within cas7e at 3600.227 kb on - strand, within cas7e at 3600.247 kb on + strand, within cas7e at 3600.262 kb on + strand, within cas7e at 3600.262 kb on + strand, within cas7e at 3600.308 kb on + strand, within cas7e at 3600.371 kb on + strand, within cas7e at 3600.399 kb on + strand, within cas7e at 3600.399 kb on + strand, within cas7e at 3600.399 kb on + strand, within cas7e at 3600.399 kb on + strand, within cas7e at 3600.400 kb on - strand, within cas7e at 3600.400 kb on - strand, within cas7e at 3600.400 kb on - strand, within cas7e at 3600.400 kb on - strand, within cas7e at 3600.418 kb on + strand, within cas7e at 3600.418 kb on + strand, within cas7e at 3600.419 kb on - strand, within cas7e at 3600.421 kb on + strand, within cas7e at 3600.422 kb on - strand, within cas7e at 3600.422 kb on - strand, within cas7e at 3600.422 kb on - strand, within cas7e at 3600.422 kb on - strand, within cas7e at 3600.422 kb on - strand, within cas7e at 3600.445 kb on + strand at 3600.445 kb on + strand at 3600.445 kb on + strand at 3600.446 kb on - strand at 3600.452 kb on + strand at 3600.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 3,598,060 - casA NIAGMN_18170 0.44 +0.3 3,598,143 + casA NIAGMN_18170 0.49 +0.4 3,598,152 + casA NIAGMN_18170 0.50 +0.5 3,598,153 - casA NIAGMN_18170 0.50 +1.1 3,598,179 + casA NIAGMN_18170 0.51 -0.2 3,598,231 + casA NIAGMN_18170 0.55 -0.4 3,598,243 + casA NIAGMN_18170 0.55 +0.9 3,598,243 + casA NIAGMN_18170 0.55 -2.3 3,598,243 + casA NIAGMN_18170 0.55 +0.4 3,598,281 - casA NIAGMN_18170 0.58 +0.4 3,598,287 + casA NIAGMN_18170 0.58 -0.6 3,598,334 + casA NIAGMN_18170 0.61 +0.4 3,598,335 - casA NIAGMN_18170 0.61 +0.1 3,598,372 - casA NIAGMN_18170 0.64 -0.5 3,598,468 + casA NIAGMN_18170 0.70 +0.3 3,598,710 - casA NIAGMN_18170 0.85 +1.2 3,598,748 + casA NIAGMN_18170 0.88 -1.2 3,598,749 - casA NIAGMN_18170 0.88 +0.6 3,598,808 + +2.2 3,598,809 - +1.7 3,598,822 + +0.4 3,598,822 + -0.6 3,598,872 + +0.4 3,599,028 - casB NIAGMN_18175 0.17 +0.4 3,599,030 + casB NIAGMN_18175 0.18 -0.4 3,599,092 + casB NIAGMN_18175 0.29 -2.3 3,599,092 + casB NIAGMN_18175 0.29 +0.2 3,599,120 + casB NIAGMN_18175 0.34 +0.5 3,599,120 + casB NIAGMN_18175 0.34 -1.4 3,599,293 + casB NIAGMN_18175 0.67 +0.4 3,599,293 + casB NIAGMN_18175 0.67 -0.7 3,599,294 - casB NIAGMN_18175 0.67 +0.6 3,599,406 - casB NIAGMN_18175 0.88 +1.9 3,599,430 + -0.0 3,599,434 - -0.1 3,599,478 - +1.9 3,599,494 + +0.2 3,599,495 - -0.5 3,599,666 - cas7e NIAGMN_18180 0.17 +0.6 3,599,686 + cas7e NIAGMN_18180 0.19 +1.0 3,599,707 + cas7e NIAGMN_18180 0.21 +1.8 3,599,838 + cas7e NIAGMN_18180 0.34 +0.2 3,599,838 + cas7e NIAGMN_18180 0.34 +0.3 3,599,871 + cas7e NIAGMN_18180 0.37 +0.9 3,599,958 + cas7e NIAGMN_18180 0.45 -1.2 3,599,958 + cas7e NIAGMN_18180 0.45 -1.1 3,599,958 + cas7e NIAGMN_18180 0.45 -0.8 3,599,958 + cas7e NIAGMN_18180 0.45 +0.5 3,599,959 - cas7e NIAGMN_18180 0.45 -0.7 3,599,968 + cas7e NIAGMN_18180 0.46 +0.5 3,599,969 - cas7e NIAGMN_18180 0.46 +0.2 3,599,980 + cas7e NIAGMN_18180 0.47 -1.4 3,599,980 + cas7e NIAGMN_18180 0.47 -1.2 3,599,981 - cas7e NIAGMN_18180 0.47 +0.2 3,599,981 - cas7e NIAGMN_18180 0.47 +0.3 3,600,162 - cas7e NIAGMN_18180 0.64 +2.6 3,600,162 - cas7e NIAGMN_18180 0.64 +0.1 3,600,162 - cas7e NIAGMN_18180 0.64 -0.4 3,600,167 + cas7e NIAGMN_18180 0.65 +1.0 3,600,167 + cas7e NIAGMN_18180 0.65 -3.6 3,600,167 + cas7e NIAGMN_18180 0.65 -1.7 3,600,167 + cas7e NIAGMN_18180 0.65 +0.2 3,600,167 + cas7e NIAGMN_18180 0.65 -0.2 3,600,168 - cas7e NIAGMN_18180 0.65 +0.9 3,600,168 - cas7e NIAGMN_18180 0.65 +0.5 3,600,168 - cas7e NIAGMN_18180 0.65 -0.9 3,600,168 - cas7e NIAGMN_18180 0.65 -0.9 3,600,169 + cas7e NIAGMN_18180 0.65 -2.2 3,600,169 + cas7e NIAGMN_18180 0.65 +0.3 3,600,169 + cas7e NIAGMN_18180 0.65 +0.2 3,600,170 - cas7e NIAGMN_18180 0.65 +0.8 3,600,170 - cas7e NIAGMN_18180 0.65 +0.6 3,600,222 + cas7e NIAGMN_18180 0.70 -0.6 3,600,226 + cas7e NIAGMN_18180 0.70 +1.5 3,600,227 - cas7e NIAGMN_18180 0.70 -1.1 3,600,227 - cas7e NIAGMN_18180 0.70 +1.6 3,600,247 + cas7e NIAGMN_18180 0.72 +1.2 3,600,262 + cas7e NIAGMN_18180 0.74 +0.1 3,600,262 + cas7e NIAGMN_18180 0.74 +0.5 3,600,308 + cas7e NIAGMN_18180 0.78 +1.2 3,600,371 + cas7e NIAGMN_18180 0.84 -0.6 3,600,399 + cas7e NIAGMN_18180 0.87 +0.9 3,600,399 + cas7e NIAGMN_18180 0.87 -0.4 3,600,399 + cas7e NIAGMN_18180 0.87 -0.2 3,600,399 + cas7e NIAGMN_18180 0.87 -0.0 3,600,400 - cas7e NIAGMN_18180 0.87 -0.3 3,600,400 - cas7e NIAGMN_18180 0.87 -1.1 3,600,400 - cas7e NIAGMN_18180 0.87 +1.0 3,600,400 - cas7e NIAGMN_18180 0.87 -0.4 3,600,418 + cas7e NIAGMN_18180 0.89 -0.1 3,600,418 + cas7e NIAGMN_18180 0.89 +0.2 3,600,419 - cas7e NIAGMN_18180 0.89 +2.1 3,600,421 + cas7e NIAGMN_18180 0.89 -0.4 3,600,422 - cas7e NIAGMN_18180 0.89 -0.9 3,600,422 - cas7e NIAGMN_18180 0.89 +0.2 3,600,422 - cas7e NIAGMN_18180 0.89 +0.3 3,600,422 - cas7e NIAGMN_18180 0.89 -0.3 3,600,422 - cas7e NIAGMN_18180 0.89 +0.1 3,600,445 + -0.1 3,600,445 + -1.9 3,600,445 + -0.2 3,600,446 - +0.0 3,600,452 + +2.1 3,600,452 + +0.8
Or see this region's nucleotide sequence