Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpiA and serA are separated by 254 nucleotides serA and NIAGMN_17235 are separated by 228 nucleotides NIAGMN_17235 and NIAGMN_17245 are separated by 333 nucleotides NIAGMN_17245 and ygfA are separated by 100 nucleotides
NIAGMN_17220: rpiA - ribose-5-phosphate isomerase RpiA, at 3,404,884 to 3,405,543
rpiA
NIAGMN_17225: serA - phosphoglycerate dehydrogenase, at 3,405,798 to 3,407,030
serA
NIAGMN_17235: NIAGMN_17235 - Type I toxin-antitoxin system Ibs family toxin, at 3,407,259 to 3,407,318
_17235
NIAGMN_17245: NIAGMN_17245 - Type I toxin-antitoxin system Ibs family toxin, at 3,407,652 to 3,407,711
_17245
NIAGMN_17250: ygfA - 5-formyltetrahydrofolate cyclo-ligase, at 3,407,812 to 3,408,414
ygfA
Position (kb)
3405
3406
3407
3408 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3404.843 kb on - strand at 3404.843 kb on - strand at 3404.844 kb on + strand at 3404.844 kb on + strand at 3404.845 kb on - strand at 3404.845 kb on - strand at 3404.845 kb on - strand at 3404.846 kb on + strand at 3404.846 kb on + strand at 3404.846 kb on + strand at 3404.846 kb on + strand at 3404.846 kb on + strand at 3404.846 kb on + strand at 3404.846 kb on + strand at 3405.659 kb on - strand at 3405.686 kb on + strand at 3405.718 kb on - strand at 3405.731 kb on + strand at 3405.742 kb on - strand at 3405.808 kb on + strand at 3405.830 kb on + strand at 3405.892 kb on - strand at 3406.031 kb on + strand, within serA at 3406.046 kb on + strand, within serA at 3406.048 kb on + strand, within serA at 3406.103 kb on + strand, within serA at 3406.121 kb on + strand, within serA at 3406.122 kb on - strand, within serA at 3406.122 kb on - strand, within serA at 3406.193 kb on + strand, within serA at 3406.280 kb on + strand, within serA at 3406.281 kb on - strand, within serA at 3406.281 kb on - strand, within serA at 3406.281 kb on - strand, within serA at 3406.323 kb on + strand, within serA at 3406.323 kb on + strand, within serA at 3406.323 kb on + strand, within serA at 3406.324 kb on - strand, within serA at 3406.324 kb on - strand, within serA at 3406.324 kb on - strand, within serA at 3406.329 kb on + strand, within serA at 3406.378 kb on + strand, within serA at 3406.403 kb on + strand, within serA at 3406.429 kb on + strand, within serA at 3406.429 kb on + strand, within serA at 3406.429 kb on + strand, within serA at 3406.429 kb on + strand, within serA at 3406.455 kb on - strand, within serA at 3406.533 kb on - strand, within serA at 3406.592 kb on - strand, within serA at 3406.616 kb on + strand, within serA at 3406.616 kb on + strand, within serA at 3406.628 kb on + strand, within serA at 3406.628 kb on + strand, within serA at 3406.629 kb on - strand, within serA at 3406.629 kb on - strand, within serA at 3406.629 kb on - strand, within serA at 3406.740 kb on + strand, within serA at 3406.902 kb on + strand, within serA at 3406.906 kb on + strand, within serA at 3406.929 kb on + strand at 3406.929 kb on + strand at 3406.933 kb on + strand at 3406.933 kb on + strand at 3406.933 kb on + strand at 3406.934 kb on - strand at 3406.934 kb on - strand at 3406.934 kb on - strand at 3406.934 kb on - strand at 3406.934 kb on - strand at 3406.934 kb on - strand at 3406.934 kb on - strand at 3406.934 kb on - strand at 3406.943 kb on + strand at 3407.026 kb on - strand at 3407.028 kb on + strand at 3407.028 kb on + strand at 3407.028 kb on + strand at 3407.029 kb on - strand at 3407.029 kb on - strand at 3407.117 kb on + strand at 3407.118 kb on - strand at 3407.188 kb on - strand at 3407.278 kb on + strand, within NIAGMN_17235 at 3407.287 kb on + strand, within NIAGMN_17235 at 3407.287 kb on + strand, within NIAGMN_17235 at 3407.287 kb on + strand, within NIAGMN_17235 at 3407.287 kb on + strand, within NIAGMN_17235 at 3407.287 kb on + strand, within NIAGMN_17235 at 3407.287 kb on + strand, within NIAGMN_17235 at 3407.293 kb on + strand, within NIAGMN_17235 at 3407.299 kb on + strand, within NIAGMN_17235 at 3407.314 kb on - strand at 3407.314 kb on - strand at 3407.314 kb on - strand at 3407.317 kb on - strand at 3407.317 kb on - strand at 3407.438 kb on - strand at 3407.459 kb on + strand at 3407.460 kb on - strand at 3407.479 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.492 kb on + strand at 3407.493 kb on - strand at 3407.493 kb on - strand at 3407.493 kb on - strand at 3407.493 kb on - strand at 3407.503 kb on + strand at 3407.503 kb on + strand at 3407.504 kb on - strand at 3407.504 kb on - strand at 3407.504 kb on - strand at 3407.565 kb on + strand at 3407.569 kb on + strand at 3407.580 kb on + strand at 3407.581 kb on - strand at 3407.671 kb on + strand, within NIAGMN_17245 at 3407.671 kb on + strand, within NIAGMN_17245 at 3407.671 kb on + strand, within NIAGMN_17245 at 3407.680 kb on + strand, within NIAGMN_17245 at 3407.680 kb on + strand, within NIAGMN_17245 at 3407.707 kb on - strand at 3407.707 kb on - strand at 3407.710 kb on - strand at 3407.744 kb on + strand at 3407.757 kb on + strand at 3407.839 kb on - strand at 3407.928 kb on + strand, within ygfA at 3407.928 kb on + strand, within ygfA at 3407.930 kb on + strand, within ygfA at 3407.930 kb on + strand, within ygfA at 3407.931 kb on - strand, within ygfA at 3407.931 kb on - strand, within ygfA at 3407.948 kb on - strand, within ygfA at 3407.994 kb on - strand, within ygfA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 3,404,843 - +1.2 3,404,843 - -0.3 3,404,844 + -1.2 3,404,844 + -1.0 3,404,845 - +1.8 3,404,845 - +1.2 3,404,845 - +0.3 3,404,846 + +0.6 3,404,846 + +0.5 3,404,846 + -0.5 3,404,846 + -2.0 3,404,846 + +0.1 3,404,846 + -0.0 3,404,846 + -0.2 3,405,659 - -0.8 3,405,686 + -1.7 3,405,718 - -2.7 3,405,731 + -4.4 3,405,742 - -3.0 3,405,808 + -3.0 3,405,830 + -3.6 3,405,892 - -1.7 3,406,031 + serA NIAGMN_17225 0.19 -5.4 3,406,046 + serA NIAGMN_17225 0.20 -2.9 3,406,048 + serA NIAGMN_17225 0.20 -1.8 3,406,103 + serA NIAGMN_17225 0.25 -5.6 3,406,121 + serA NIAGMN_17225 0.26 -4.4 3,406,122 - serA NIAGMN_17225 0.26 -3.3 3,406,122 - serA NIAGMN_17225 0.26 -4.8 3,406,193 + serA NIAGMN_17225 0.32 -2.7 3,406,280 + serA NIAGMN_17225 0.39 -3.2 3,406,281 - serA NIAGMN_17225 0.39 -2.0 3,406,281 - serA NIAGMN_17225 0.39 -4.1 3,406,281 - serA NIAGMN_17225 0.39 -0.7 3,406,323 + serA NIAGMN_17225 0.43 -4.2 3,406,323 + serA NIAGMN_17225 0.43 -4.0 3,406,323 + serA NIAGMN_17225 0.43 -5.9 3,406,324 - serA NIAGMN_17225 0.43 -1.6 3,406,324 - serA NIAGMN_17225 0.43 -1.6 3,406,324 - serA NIAGMN_17225 0.43 -3.9 3,406,329 + serA NIAGMN_17225 0.43 -3.2 3,406,378 + serA NIAGMN_17225 0.47 -4.9 3,406,403 + serA NIAGMN_17225 0.49 -4.9 3,406,429 + serA NIAGMN_17225 0.51 -2.2 3,406,429 + serA NIAGMN_17225 0.51 -2.4 3,406,429 + serA NIAGMN_17225 0.51 -4.3 3,406,429 + serA NIAGMN_17225 0.51 -3.5 3,406,455 - serA NIAGMN_17225 0.53 -3.4 3,406,533 - serA NIAGMN_17225 0.60 -3.6 3,406,592 - serA NIAGMN_17225 0.64 -3.4 3,406,616 + serA NIAGMN_17225 0.66 -1.6 3,406,616 + serA NIAGMN_17225 0.66 -4.4 3,406,628 + serA NIAGMN_17225 0.67 -2.4 3,406,628 + serA NIAGMN_17225 0.67 -5.4 3,406,629 - serA NIAGMN_17225 0.67 -3.3 3,406,629 - serA NIAGMN_17225 0.67 -3.5 3,406,629 - serA NIAGMN_17225 0.67 -3.4 3,406,740 + serA NIAGMN_17225 0.76 -4.0 3,406,902 + serA NIAGMN_17225 0.90 -3.8 3,406,906 + serA NIAGMN_17225 0.90 -3.5 3,406,929 + -3.6 3,406,929 + -2.5 3,406,933 + -4.5 3,406,933 + -4.9 3,406,933 + -2.0 3,406,934 - -1.6 3,406,934 - -2.5 3,406,934 - -5.9 3,406,934 - -2.7 3,406,934 - -2.5 3,406,934 - -2.7 3,406,934 - -0.3 3,406,934 - -3.9 3,406,943 + -3.5 3,407,026 - -0.2 3,407,028 + +0.4 3,407,028 + +0.3 3,407,028 + +0.8 3,407,029 - -0.7 3,407,029 - +1.2 3,407,117 + -0.4 3,407,118 - -0.5 3,407,188 - +0.1 3,407,278 + NIAGMN_17235 0.32 -0.2 3,407,287 + NIAGMN_17235 0.47 -2.1 3,407,287 + NIAGMN_17235 0.47 -0.2 3,407,287 + NIAGMN_17235 0.47 +1.2 3,407,287 + NIAGMN_17235 0.47 -1.4 3,407,287 + NIAGMN_17235 0.47 +0.5 3,407,287 + NIAGMN_17235 0.47 -1.4 3,407,293 + NIAGMN_17235 0.57 -2.3 3,407,299 + NIAGMN_17235 0.67 -0.2 3,407,314 - -1.3 3,407,314 - +0.6 3,407,314 - -0.3 3,407,317 - -0.6 3,407,317 - -0.7 3,407,438 - +0.3 3,407,459 + -0.0 3,407,460 - -1.0 3,407,479 + +1.8 3,407,492 + +0.3 3,407,492 + -0.3 3,407,492 + +0.3 3,407,492 + -0.5 3,407,492 + -1.7 3,407,492 + +2.2 3,407,492 + -0.3 3,407,492 + +1.3 3,407,492 + -0.4 3,407,492 + +0.4 3,407,492 + -0.3 3,407,492 + +0.1 3,407,493 - -3.2 3,407,493 - +0.2 3,407,493 - -0.7 3,407,493 - +1.6 3,407,503 + -0.0 3,407,503 + -2.7 3,407,504 - +1.0 3,407,504 - +0.5 3,407,504 - +0.5 3,407,565 + +0.5 3,407,569 + -1.2 3,407,580 + +0.5 3,407,581 - -0.2 3,407,671 + NIAGMN_17245 0.32 +0.6 3,407,671 + NIAGMN_17245 0.32 -1.7 3,407,671 + NIAGMN_17245 0.32 +0.1 3,407,680 + NIAGMN_17245 0.47 -0.4 3,407,680 + NIAGMN_17245 0.47 -1.5 3,407,707 - +1.1 3,407,707 - +0.2 3,407,710 - +0.1 3,407,744 + +0.2 3,407,757 + +0.1 3,407,839 - +0.6 3,407,928 + ygfA NIAGMN_17250 0.19 +2.0 3,407,928 + ygfA NIAGMN_17250 0.19 +0.8 3,407,930 + ygfA NIAGMN_17250 0.20 +1.4 3,407,930 + ygfA NIAGMN_17250 0.20 -0.5 3,407,931 - ygfA NIAGMN_17250 0.20 -0.3 3,407,931 - ygfA NIAGMN_17250 0.20 -2.2 3,407,948 - ygfA NIAGMN_17250 0.23 -1.1 3,407,994 - ygfA NIAGMN_17250 0.30 -0.1
Or see this region's nucleotide sequence