Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and thiE overlap by 1 nucleotides thiE and thiF overlap by 8 nucleotides thiF and thiS overlap by 17 nucleotides thiS and thiG are separated by 1 nucleotides thiG and thiH overlap by 4 nucleotides
NIAGMN_11125: thiC - phosphomethylpyrimidine synthase ThiC, at 2,196,800 to 2,198,695
thiC
NIAGMN_11130: thiE - thiamine phosphate synthase, at 2,198,695 to 2,199,330
thiE
NIAGMN_11135: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 2,199,323 to 2,200,078
thiF
NIAGMN_11140: thiS - sulfur carrier protein ThiS, at 2,200,062 to 2,200,262
thiS
NIAGMN_11145: thiG - thiazole synthase, at 2,200,264 to 2,201,034
thiG
NIAGMN_11150: thiH - 2-iminoacetate synthase ThiH, at 2,201,031 to 2,202,164
thiH
Position (kb)
2199
2200
2201 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2198.341 kb on + strand, within thiC at 2198.342 kb on - strand, within thiC at 2198.348 kb on + strand, within thiC at 2198.348 kb on + strand, within thiC at 2198.348 kb on + strand, within thiC at 2198.348 kb on + strand, within thiC at 2198.348 kb on + strand, within thiC at 2198.349 kb on - strand, within thiC at 2198.349 kb on - strand, within thiC at 2198.349 kb on - strand, within thiC at 2198.349 kb on - strand, within thiC at 2198.350 kb on + strand, within thiC at 2198.350 kb on + strand, within thiC at 2198.351 kb on - strand, within thiC at 2198.458 kb on + strand, within thiC at 2198.459 kb on - strand, within thiC at 2198.524 kb on + strand at 2198.524 kb on + strand at 2198.614 kb on + strand at 2198.615 kb on - strand at 2198.615 kb on - strand at 2198.635 kb on + strand at 2198.673 kb on - strand at 2198.680 kb on + strand at 2198.698 kb on + strand at 2198.698 kb on + strand at 2198.698 kb on + strand at 2198.699 kb on - strand at 2198.699 kb on - strand at 2198.699 kb on - strand at 2198.699 kb on - strand at 2198.721 kb on - strand at 2198.741 kb on - strand at 2198.759 kb on + strand, within thiE at 2198.759 kb on + strand, within thiE at 2198.759 kb on + strand, within thiE at 2198.759 kb on + strand, within thiE at 2198.759 kb on + strand, within thiE at 2198.760 kb on - strand, within thiE at 2198.760 kb on - strand, within thiE at 2198.760 kb on - strand, within thiE at 2198.760 kb on - strand, within thiE at 2198.792 kb on + strand, within thiE at 2198.792 kb on + strand, within thiE at 2198.792 kb on + strand, within thiE at 2198.793 kb on - strand, within thiE at 2198.793 kb on - strand, within thiE at 2198.796 kb on + strand, within thiE at 2198.796 kb on + strand, within thiE at 2198.796 kb on + strand, within thiE at 2198.796 kb on + strand, within thiE at 2198.796 kb on + strand, within thiE at 2198.796 kb on + strand, within thiE at 2198.796 kb on + strand, within thiE at 2198.797 kb on - strand, within thiE at 2198.797 kb on - strand, within thiE at 2198.797 kb on - strand, within thiE at 2198.807 kb on + strand, within thiE at 2198.807 kb on + strand, within thiE at 2198.808 kb on - strand, within thiE at 2198.808 kb on - strand, within thiE at 2198.808 kb on - strand, within thiE at 2198.879 kb on - strand, within thiE at 2198.880 kb on + strand, within thiE at 2198.881 kb on - strand, within thiE at 2198.895 kb on + strand, within thiE at 2198.896 kb on - strand, within thiE at 2198.936 kb on - strand, within thiE at 2198.936 kb on - strand, within thiE at 2199.067 kb on + strand, within thiE at 2199.067 kb on + strand, within thiE at 2199.067 kb on + strand, within thiE at 2199.067 kb on + strand, within thiE at 2199.068 kb on - strand, within thiE at 2199.069 kb on + strand, within thiE at 2199.069 kb on + strand, within thiE at 2199.069 kb on + strand, within thiE at 2199.069 kb on + strand, within thiE at 2199.070 kb on - strand, within thiE at 2199.169 kb on + strand, within thiE at 2199.456 kb on + strand, within thiF at 2199.456 kb on + strand, within thiF at 2199.457 kb on - strand, within thiF at 2199.458 kb on + strand, within thiF at 2199.458 kb on + strand, within thiF at 2199.516 kb on + strand, within thiF at 2199.516 kb on + strand, within thiF at 2199.517 kb on - strand, within thiF at 2199.517 kb on - strand, within thiF at 2199.630 kb on + strand, within thiF at 2199.630 kb on + strand, within thiF at 2199.630 kb on + strand, within thiF at 2199.630 kb on + strand, within thiF at 2199.630 kb on + strand, within thiF at 2199.630 kb on + strand, within thiF at 2199.630 kb on + strand, within thiF at 2199.631 kb on - strand, within thiF at 2199.631 kb on - strand, within thiF at 2199.631 kb on - strand, within thiF at 2199.707 kb on - strand, within thiF at 2199.727 kb on + strand, within thiF at 2199.728 kb on - strand, within thiF at 2199.728 kb on - strand, within thiF at 2199.760 kb on + strand, within thiF at 2199.802 kb on - strand, within thiF at 2199.940 kb on + strand, within thiF at 2199.940 kb on + strand, within thiF at 2199.940 kb on + strand, within thiF at 2199.941 kb on - strand, within thiF at 2199.945 kb on + strand, within thiF at 2199.945 kb on + strand, within thiF at 2199.946 kb on - strand, within thiF at 2199.958 kb on - strand, within thiF at 2199.995 kb on - strand, within thiF at 2200.123 kb on + strand, within thiS at 2200.123 kb on + strand, within thiS at 2200.124 kb on - strand, within thiS at 2200.169 kb on + strand, within thiS at 2200.208 kb on + strand, within thiS at 2200.208 kb on + strand, within thiS at 2200.208 kb on + strand, within thiS at 2200.208 kb on + strand, within thiS at 2200.209 kb on - strand, within thiS at 2200.247 kb on + strand at 2200.248 kb on - strand at 2200.268 kb on + strand at 2200.272 kb on + strand at 2200.272 kb on + strand at 2200.273 kb on - strand at 2200.273 kb on - strand at 2200.343 kb on + strand, within thiG at 2200.343 kb on + strand, within thiG at 2200.467 kb on + strand, within thiG at 2200.467 kb on + strand, within thiG at 2200.467 kb on + strand, within thiG at 2200.467 kb on + strand, within thiG at 2200.468 kb on - strand, within thiG at 2200.623 kb on - strand, within thiG at 2200.675 kb on - strand, within thiG at 2200.770 kb on + strand, within thiG at 2200.770 kb on + strand, within thiG at 2200.770 kb on + strand, within thiG at 2200.771 kb on - strand, within thiG at 2200.771 kb on - strand, within thiG at 2200.771 kb on - strand, within thiG at 2200.771 kb on - strand, within thiG at 2200.868 kb on + strand, within thiG at 2200.876 kb on - strand, within thiG at 2200.934 kb on + strand, within thiG at 2200.981 kb on + strand at 2200.981 kb on + strand at 2200.981 kb on + strand at 2201.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,198,341 + thiC NIAGMN_11125 0.81 +2.3 2,198,342 - thiC NIAGMN_11125 0.81 -0.1 2,198,348 + thiC NIAGMN_11125 0.82 +0.1 2,198,348 + thiC NIAGMN_11125 0.82 -0.2 2,198,348 + thiC NIAGMN_11125 0.82 -1.7 2,198,348 + thiC NIAGMN_11125 0.82 +0.0 2,198,348 + thiC NIAGMN_11125 0.82 -0.7 2,198,349 - thiC NIAGMN_11125 0.82 -0.1 2,198,349 - thiC NIAGMN_11125 0.82 -0.2 2,198,349 - thiC NIAGMN_11125 0.82 -0.4 2,198,349 - thiC NIAGMN_11125 0.82 +0.1 2,198,350 + thiC NIAGMN_11125 0.82 -0.7 2,198,350 + thiC NIAGMN_11125 0.82 -0.1 2,198,351 - thiC NIAGMN_11125 0.82 -0.7 2,198,458 + thiC NIAGMN_11125 0.87 -0.6 2,198,459 - thiC NIAGMN_11125 0.88 +0.5 2,198,524 + -0.3 2,198,524 + +0.4 2,198,614 + +0.1 2,198,615 - +0.4 2,198,615 - +0.1 2,198,635 + -0.4 2,198,673 - +0.4 2,198,680 + -1.8 2,198,698 + +1.2 2,198,698 + -2.8 2,198,698 + -0.5 2,198,699 - +0.3 2,198,699 - -1.8 2,198,699 - +0.2 2,198,699 - -1.8 2,198,721 - -1.4 2,198,741 - -0.3 2,198,759 + thiE NIAGMN_11130 0.10 +0.5 2,198,759 + thiE NIAGMN_11130 0.10 -2.4 2,198,759 + thiE NIAGMN_11130 0.10 +0.1 2,198,759 + thiE NIAGMN_11130 0.10 -0.1 2,198,759 + thiE NIAGMN_11130 0.10 +0.8 2,198,760 - thiE NIAGMN_11130 0.10 +0.3 2,198,760 - thiE NIAGMN_11130 0.10 -0.1 2,198,760 - thiE NIAGMN_11130 0.10 -0.7 2,198,760 - thiE NIAGMN_11130 0.10 +0.1 2,198,792 + thiE NIAGMN_11130 0.15 -1.5 2,198,792 + thiE NIAGMN_11130 0.15 +0.1 2,198,792 + thiE NIAGMN_11130 0.15 -0.4 2,198,793 - thiE NIAGMN_11130 0.15 -0.2 2,198,793 - thiE NIAGMN_11130 0.15 +0.3 2,198,796 + thiE NIAGMN_11130 0.16 -0.4 2,198,796 + thiE NIAGMN_11130 0.16 -0.9 2,198,796 + thiE NIAGMN_11130 0.16 -0.0 2,198,796 + thiE NIAGMN_11130 0.16 -0.1 2,198,796 + thiE NIAGMN_11130 0.16 +1.1 2,198,796 + thiE NIAGMN_11130 0.16 +0.0 2,198,796 + thiE NIAGMN_11130 0.16 -0.8 2,198,797 - thiE NIAGMN_11130 0.16 +0.2 2,198,797 - thiE NIAGMN_11130 0.16 -2.4 2,198,797 - thiE NIAGMN_11130 0.16 -0.2 2,198,807 + thiE NIAGMN_11130 0.18 +0.0 2,198,807 + thiE NIAGMN_11130 0.18 +0.0 2,198,808 - thiE NIAGMN_11130 0.18 -0.9 2,198,808 - thiE NIAGMN_11130 0.18 +0.6 2,198,808 - thiE NIAGMN_11130 0.18 -0.7 2,198,879 - thiE NIAGMN_11130 0.29 -0.4 2,198,880 + thiE NIAGMN_11130 0.29 -1.5 2,198,881 - thiE NIAGMN_11130 0.29 -1.5 2,198,895 + thiE NIAGMN_11130 0.31 -0.7 2,198,896 - thiE NIAGMN_11130 0.32 +0.9 2,198,936 - thiE NIAGMN_11130 0.38 -1.0 2,198,936 - thiE NIAGMN_11130 0.38 -0.9 2,199,067 + thiE NIAGMN_11130 0.58 -0.4 2,199,067 + thiE NIAGMN_11130 0.58 -1.1 2,199,067 + thiE NIAGMN_11130 0.58 -0.6 2,199,067 + thiE NIAGMN_11130 0.58 -1.2 2,199,068 - thiE NIAGMN_11130 0.59 +0.0 2,199,069 + thiE NIAGMN_11130 0.59 -0.7 2,199,069 + thiE NIAGMN_11130 0.59 +1.5 2,199,069 + thiE NIAGMN_11130 0.59 +0.3 2,199,069 + thiE NIAGMN_11130 0.59 -1.2 2,199,070 - thiE NIAGMN_11130 0.59 -1.4 2,199,169 + thiE NIAGMN_11130 0.75 -0.1 2,199,456 + thiF NIAGMN_11135 0.18 -0.2 2,199,456 + thiF NIAGMN_11135 0.18 -0.7 2,199,457 - thiF NIAGMN_11135 0.18 -1.5 2,199,458 + thiF NIAGMN_11135 0.18 -0.4 2,199,458 + thiF NIAGMN_11135 0.18 +0.1 2,199,516 + thiF NIAGMN_11135 0.26 -0.4 2,199,516 + thiF NIAGMN_11135 0.26 +0.1 2,199,517 - thiF NIAGMN_11135 0.26 -0.7 2,199,517 - thiF NIAGMN_11135 0.26 -0.4 2,199,630 + thiF NIAGMN_11135 0.41 -0.6 2,199,630 + thiF NIAGMN_11135 0.41 -0.6 2,199,630 + thiF NIAGMN_11135 0.41 -0.6 2,199,630 + thiF NIAGMN_11135 0.41 +1.0 2,199,630 + thiF NIAGMN_11135 0.41 +0.1 2,199,630 + thiF NIAGMN_11135 0.41 +1.6 2,199,630 + thiF NIAGMN_11135 0.41 +0.2 2,199,631 - thiF NIAGMN_11135 0.41 +0.5 2,199,631 - thiF NIAGMN_11135 0.41 +0.6 2,199,631 - thiF NIAGMN_11135 0.41 -0.5 2,199,707 - thiF NIAGMN_11135 0.51 -0.6 2,199,727 + thiF NIAGMN_11135 0.53 -0.4 2,199,728 - thiF NIAGMN_11135 0.54 -1.2 2,199,728 - thiF NIAGMN_11135 0.54 -1.9 2,199,760 + thiF NIAGMN_11135 0.58 -1.5 2,199,802 - thiF NIAGMN_11135 0.63 -0.1 2,199,940 + thiF NIAGMN_11135 0.82 -1.0 2,199,940 + thiF NIAGMN_11135 0.82 -1.0 2,199,940 + thiF NIAGMN_11135 0.82 -0.5 2,199,941 - thiF NIAGMN_11135 0.82 +0.5 2,199,945 + thiF NIAGMN_11135 0.82 +0.2 2,199,945 + thiF NIAGMN_11135 0.82 -1.2 2,199,946 - thiF NIAGMN_11135 0.82 -1.5 2,199,958 - thiF NIAGMN_11135 0.84 -1.1 2,199,995 - thiF NIAGMN_11135 0.89 +0.4 2,200,123 + thiS NIAGMN_11140 0.30 -0.5 2,200,123 + thiS NIAGMN_11140 0.30 -0.1 2,200,124 - thiS NIAGMN_11140 0.31 +0.2 2,200,169 + thiS NIAGMN_11140 0.53 -0.7 2,200,208 + thiS NIAGMN_11140 0.73 +0.3 2,200,208 + thiS NIAGMN_11140 0.73 -0.7 2,200,208 + thiS NIAGMN_11140 0.73 -0.3 2,200,208 + thiS NIAGMN_11140 0.73 -0.4 2,200,209 - thiS NIAGMN_11140 0.73 -1.0 2,200,247 + +0.9 2,200,248 - +0.4 2,200,268 + +0.3 2,200,272 + -0.8 2,200,272 + +0.8 2,200,273 - +0.3 2,200,273 - -0.4 2,200,343 + thiG NIAGMN_11145 0.10 +0.0 2,200,343 + thiG NIAGMN_11145 0.10 -0.0 2,200,467 + thiG NIAGMN_11145 0.26 -1.4 2,200,467 + thiG NIAGMN_11145 0.26 -0.5 2,200,467 + thiG NIAGMN_11145 0.26 -0.5 2,200,467 + thiG NIAGMN_11145 0.26 -2.1 2,200,468 - thiG NIAGMN_11145 0.26 +0.8 2,200,623 - thiG NIAGMN_11145 0.47 -0.6 2,200,675 - thiG NIAGMN_11145 0.53 +0.5 2,200,770 + thiG NIAGMN_11145 0.66 -0.5 2,200,770 + thiG NIAGMN_11145 0.66 +0.4 2,200,770 + thiG NIAGMN_11145 0.66 +2.0 2,200,771 - thiG NIAGMN_11145 0.66 -2.6 2,200,771 - thiG NIAGMN_11145 0.66 +2.8 2,200,771 - thiG NIAGMN_11145 0.66 -1.6 2,200,771 - thiG NIAGMN_11145 0.66 -1.5 2,200,868 + thiG NIAGMN_11145 0.78 -0.6 2,200,876 - thiG NIAGMN_11145 0.79 -0.8 2,200,934 + thiG NIAGMN_11145 0.87 -1.6 2,200,981 + -1.7 2,200,981 + +0.4 2,200,981 + -1.0 2,201,030 + -0.5
Or see this region's nucleotide sequence