Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpC and mhpB are separated by 17 nucleotides mhpB and mhpA are separated by 1 nucleotides mhpA and mhpR are separated by 76 nucleotides
NIAGMN_06830: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 1,310,026 to 1,310,892
mhpC
NIAGMN_06835: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 1,310,910 to 1,311,854
mhpB
NIAGMN_06840: mhpA - bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, at 1,311,856 to 1,313,520
mhpA
NIAGMN_06845: mhpR - DNA-binding transcriptional regulator, at 1,313,597 to 1,314,544
mhpR
Position (kb)
1311
1312
1313
1314 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1310.975 kb on - strand at 1311.012 kb on - strand, within mhpB at 1311.106 kb on - strand, within mhpB at 1311.106 kb on - strand, within mhpB at 1311.204 kb on - strand, within mhpB at 1311.204 kb on - strand, within mhpB at 1311.278 kb on + strand, within mhpB at 1311.278 kb on + strand, within mhpB at 1311.279 kb on - strand, within mhpB at 1311.356 kb on + strand, within mhpB at 1311.524 kb on + strand, within mhpB at 1311.524 kb on + strand, within mhpB at 1311.524 kb on + strand, within mhpB at 1311.525 kb on - strand, within mhpB at 1311.525 kb on - strand, within mhpB at 1311.525 kb on - strand, within mhpB at 1311.525 kb on - strand, within mhpB at 1311.525 kb on - strand, within mhpB at 1311.530 kb on - strand, within mhpB at 1311.679 kb on + strand, within mhpB at 1311.680 kb on - strand, within mhpB at 1311.744 kb on - strand, within mhpB at 1311.786 kb on + strand at 1311.787 kb on - strand at 1311.808 kb on + strand at 1311.844 kb on + strand at 1311.844 kb on + strand at 1311.844 kb on + strand at 1311.845 kb on - strand at 1311.845 kb on - strand at 1311.863 kb on - strand at 1312.020 kb on + strand at 1312.051 kb on + strand, within mhpA at 1312.189 kb on + strand, within mhpA at 1312.264 kb on - strand, within mhpA at 1312.361 kb on + strand, within mhpA at 1312.416 kb on + strand, within mhpA at 1312.417 kb on - strand, within mhpA at 1312.417 kb on - strand, within mhpA at 1312.509 kb on - strand, within mhpA at 1312.509 kb on - strand, within mhpA at 1312.573 kb on - strand, within mhpA at 1312.588 kb on - strand, within mhpA at 1312.596 kb on + strand, within mhpA at 1312.597 kb on - strand, within mhpA at 1312.598 kb on + strand, within mhpA at 1312.663 kb on - strand, within mhpA at 1312.698 kb on - strand, within mhpA at 1312.813 kb on - strand, within mhpA at 1312.883 kb on + strand, within mhpA at 1312.883 kb on + strand, within mhpA at 1312.883 kb on + strand, within mhpA at 1312.883 kb on + strand, within mhpA at 1312.883 kb on + strand, within mhpA at 1312.883 kb on + strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.884 kb on - strand, within mhpA at 1312.887 kb on + strand, within mhpA at 1312.887 kb on + strand, within mhpA at 1312.888 kb on - strand, within mhpA at 1312.888 kb on - strand, within mhpA at 1312.896 kb on + strand, within mhpA at 1312.897 kb on - strand, within mhpA at 1312.917 kb on + strand, within mhpA at 1313.227 kb on - strand, within mhpA at 1313.281 kb on - strand, within mhpA at 1313.284 kb on - strand, within mhpA at 1313.347 kb on - strand, within mhpA at 1313.347 kb on - strand, within mhpA at 1313.423 kb on + strand at 1313.423 kb on + strand at 1313.512 kb on + strand at 1313.513 kb on - strand at 1313.564 kb on + strand at 1313.565 kb on - strand at 1313.889 kb on - strand, within mhpR at 1313.889 kb on - strand, within mhpR at 1313.915 kb on - strand, within mhpR at 1313.915 kb on - strand, within mhpR at 1314.024 kb on + strand, within mhpR at 1314.236 kb on + strand, within mhpR at 1314.267 kb on - strand, within mhpR at 1314.299 kb on + strand, within mhpR at 1314.401 kb on + strand, within mhpR at 1314.401 kb on + strand, within mhpR at 1314.401 kb on + strand, within mhpR at 1314.401 kb on + strand, within mhpR at 1314.401 kb on + strand, within mhpR at 1314.401 kb on + strand, within mhpR at 1314.402 kb on - strand, within mhpR at 1314.403 kb on + strand, within mhpR at 1314.403 kb on + strand, within mhpR at 1314.404 kb on - strand, within mhpR at 1314.456 kb on + strand at 1314.457 kb on - strand at 1314.502 kb on + strand at 1314.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,310,975 - +1.2 1,311,012 - mhpB NIAGMN_06835 0.11 +0.6 1,311,106 - mhpB NIAGMN_06835 0.21 -2.2 1,311,106 - mhpB NIAGMN_06835 0.21 +0.3 1,311,204 - mhpB NIAGMN_06835 0.31 +1.0 1,311,204 - mhpB NIAGMN_06835 0.31 -2.6 1,311,278 + mhpB NIAGMN_06835 0.39 +0.3 1,311,278 + mhpB NIAGMN_06835 0.39 +0.4 1,311,279 - mhpB NIAGMN_06835 0.39 +0.5 1,311,356 + mhpB NIAGMN_06835 0.47 -2.5 1,311,524 + mhpB NIAGMN_06835 0.65 -1.6 1,311,524 + mhpB NIAGMN_06835 0.65 +1.4 1,311,524 + mhpB NIAGMN_06835 0.65 +0.2 1,311,525 - mhpB NIAGMN_06835 0.65 -0.3 1,311,525 - mhpB NIAGMN_06835 0.65 -1.7 1,311,525 - mhpB NIAGMN_06835 0.65 -0.4 1,311,525 - mhpB NIAGMN_06835 0.65 -0.1 1,311,525 - mhpB NIAGMN_06835 0.65 +0.7 1,311,530 - mhpB NIAGMN_06835 0.66 -0.5 1,311,679 + mhpB NIAGMN_06835 0.81 -1.6 1,311,680 - mhpB NIAGMN_06835 0.81 +1.5 1,311,744 - mhpB NIAGMN_06835 0.88 +0.2 1,311,786 + +0.9 1,311,787 - -0.1 1,311,808 + +0.2 1,311,844 + +0.3 1,311,844 + -0.6 1,311,844 + +0.3 1,311,845 - +0.2 1,311,845 - +0.3 1,311,863 - +0.1 1,312,020 + -0.1 1,312,051 + mhpA NIAGMN_06840 0.12 -1.2 1,312,189 + mhpA NIAGMN_06840 0.20 +0.9 1,312,264 - mhpA NIAGMN_06840 0.25 -0.3 1,312,361 + mhpA NIAGMN_06840 0.30 -3.5 1,312,416 + mhpA NIAGMN_06840 0.34 +1.1 1,312,417 - mhpA NIAGMN_06840 0.34 -0.5 1,312,417 - mhpA NIAGMN_06840 0.34 -0.8 1,312,509 - mhpA NIAGMN_06840 0.39 -0.2 1,312,509 - mhpA NIAGMN_06840 0.39 -0.0 1,312,573 - mhpA NIAGMN_06840 0.43 -0.6 1,312,588 - mhpA NIAGMN_06840 0.44 +0.0 1,312,596 + mhpA NIAGMN_06840 0.44 +1.7 1,312,597 - mhpA NIAGMN_06840 0.45 +0.2 1,312,598 + mhpA NIAGMN_06840 0.45 -1.4 1,312,663 - mhpA NIAGMN_06840 0.48 +0.8 1,312,698 - mhpA NIAGMN_06840 0.51 -0.5 1,312,813 - mhpA NIAGMN_06840 0.57 -1.9 1,312,883 + mhpA NIAGMN_06840 0.62 +1.7 1,312,883 + mhpA NIAGMN_06840 0.62 -0.2 1,312,883 + mhpA NIAGMN_06840 0.62 +0.3 1,312,883 + mhpA NIAGMN_06840 0.62 +1.0 1,312,883 + mhpA NIAGMN_06840 0.62 +0.9 1,312,883 + mhpA NIAGMN_06840 0.62 -3.3 1,312,884 - mhpA NIAGMN_06840 0.62 -1.1 1,312,884 - mhpA NIAGMN_06840 0.62 +0.5 1,312,884 - mhpA NIAGMN_06840 0.62 -0.5 1,312,884 - mhpA NIAGMN_06840 0.62 +2.0 1,312,884 - mhpA NIAGMN_06840 0.62 -0.2 1,312,884 - mhpA NIAGMN_06840 0.62 +0.1 1,312,884 - mhpA NIAGMN_06840 0.62 +1.0 1,312,884 - mhpA NIAGMN_06840 0.62 +1.0 1,312,884 - mhpA NIAGMN_06840 0.62 +0.6 1,312,884 - mhpA NIAGMN_06840 0.62 +0.1 1,312,887 + mhpA NIAGMN_06840 0.62 -0.6 1,312,887 + mhpA NIAGMN_06840 0.62 -0.6 1,312,888 - mhpA NIAGMN_06840 0.62 +1.3 1,312,888 - mhpA NIAGMN_06840 0.62 +1.0 1,312,896 + mhpA NIAGMN_06840 0.62 -0.8 1,312,897 - mhpA NIAGMN_06840 0.63 +0.2 1,312,917 + mhpA NIAGMN_06840 0.64 -0.6 1,313,227 - mhpA NIAGMN_06840 0.82 -0.9 1,313,281 - mhpA NIAGMN_06840 0.86 +0.7 1,313,284 - mhpA NIAGMN_06840 0.86 +0.7 1,313,347 - mhpA NIAGMN_06840 0.90 -1.8 1,313,347 - mhpA NIAGMN_06840 0.90 -0.8 1,313,423 + -1.1 1,313,423 + -0.8 1,313,512 + +0.7 1,313,513 - -0.1 1,313,564 + -0.1 1,313,565 - +0.0 1,313,889 - mhpR NIAGMN_06845 0.31 -3.1 1,313,889 - mhpR NIAGMN_06845 0.31 +2.0 1,313,915 - mhpR NIAGMN_06845 0.34 -0.2 1,313,915 - mhpR NIAGMN_06845 0.34 -2.3 1,314,024 + mhpR NIAGMN_06845 0.45 +1.2 1,314,236 + mhpR NIAGMN_06845 0.67 -0.5 1,314,267 - mhpR NIAGMN_06845 0.71 -2.0 1,314,299 + mhpR NIAGMN_06845 0.74 -1.2 1,314,401 + mhpR NIAGMN_06845 0.85 -1.5 1,314,401 + mhpR NIAGMN_06845 0.85 -0.6 1,314,401 + mhpR NIAGMN_06845 0.85 -1.1 1,314,401 + mhpR NIAGMN_06845 0.85 +0.2 1,314,401 + mhpR NIAGMN_06845 0.85 +0.6 1,314,401 + mhpR NIAGMN_06845 0.85 -1.7 1,314,402 - mhpR NIAGMN_06845 0.85 +1.1 1,314,403 + mhpR NIAGMN_06845 0.85 -1.0 1,314,403 + mhpR NIAGMN_06845 0.85 +1.0 1,314,404 - mhpR NIAGMN_06845 0.85 +1.5 1,314,456 + -0.5 1,314,457 - -0.1 1,314,502 + +0.5 1,314,503 - +1.7
Or see this region's nucleotide sequence