Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdK and ybdJ are separated by 113 nucleotides ybdJ and ybdF are separated by 64 nucleotides ybdF and nfsB are separated by 93 nucleotides nfsB and ybdG are separated by 107 nucleotides
NIAGMN_05710: ybdK - YbdK family carboxylate-amine ligase, at 1,051,141 to 1,052,259
ybdK
NIAGMN_05715: ybdJ - Uncharacterized protein YbdJ, at 1,052,373 to 1,052,573
ybdJ
NIAGMN_05720: ybdF - MmcQ/YjbR family DNA-binding protein, at 1,052,638 to 1,053,006
ybdF
NIAGMN_05725: nfsB - oxygen-insensitive NAD(P)H nitroreductase, at 1,053,100 to 1,053,753
nfsB
NIAGMN_05730: ybdG - mechanosensitive ion channel YbdG, at 1,053,861 to 1,055,108
ybdG
Position (kb)
1052
1053
1054 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1051.648 kb on + strand, within ybdK at 1051.648 kb on + strand, within ybdK at 1051.650 kb on + strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.738 kb on - strand, within ybdK at 1051.777 kb on + strand, within ybdK at 1051.777 kb on + strand, within ybdK at 1051.893 kb on - strand, within ybdK at 1051.957 kb on - strand, within ybdK at 1051.978 kb on + strand, within ybdK at 1052.038 kb on + strand, within ybdK at 1052.109 kb on + strand, within ybdK at 1052.257 kb on + strand at 1052.275 kb on - strand at 1052.275 kb on - strand at 1052.278 kb on + strand at 1052.316 kb on - strand at 1052.612 kb on - strand at 1052.688 kb on - strand, within ybdF at 1052.837 kb on + strand, within ybdF at 1052.839 kb on + strand, within ybdF at 1053.059 kb on + strand at 1053.059 kb on + strand at 1053.080 kb on + strand at 1053.244 kb on - strand, within nfsB at 1053.267 kb on + strand, within nfsB at 1053.298 kb on - strand, within nfsB at 1053.301 kb on + strand, within nfsB at 1053.301 kb on + strand, within nfsB at 1053.301 kb on + strand, within nfsB at 1053.302 kb on - strand, within nfsB at 1053.319 kb on - strand, within nfsB at 1053.323 kb on + strand, within nfsB at 1053.323 kb on + strand, within nfsB at 1053.646 kb on + strand, within nfsB at 1053.736 kb on - strand at 1053.751 kb on + strand at 1053.751 kb on + strand at 1053.751 kb on + strand at 1053.782 kb on - strand at 1053.833 kb on + strand at 1053.834 kb on - strand at 1053.834 kb on - strand at 1053.846 kb on + strand at 1053.846 kb on + strand at 1053.846 kb on + strand at 1053.847 kb on - strand at 1053.847 kb on - strand at 1053.849 kb on - strand at 1053.871 kb on + strand at 1053.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,051,648 + ybdK NIAGMN_05710 0.45 +0.3 1,051,648 + ybdK NIAGMN_05710 0.45 -1.4 1,051,650 + ybdK NIAGMN_05710 0.45 -1.1 1,051,651 - ybdK NIAGMN_05710 0.46 -1.1 1,051,651 - ybdK NIAGMN_05710 0.46 +0.3 1,051,651 - ybdK NIAGMN_05710 0.46 +1.3 1,051,651 - ybdK NIAGMN_05710 0.46 +1.2 1,051,651 - ybdK NIAGMN_05710 0.46 -0.7 1,051,651 - ybdK NIAGMN_05710 0.46 -0.1 1,051,651 - ybdK NIAGMN_05710 0.46 +1.7 1,051,651 - ybdK NIAGMN_05710 0.46 +0.3 1,051,651 - ybdK NIAGMN_05710 0.46 +0.1 1,051,651 - ybdK NIAGMN_05710 0.46 +0.5 1,051,651 - ybdK NIAGMN_05710 0.46 +1.0 1,051,738 - ybdK NIAGMN_05710 0.53 -2.0 1,051,777 + ybdK NIAGMN_05710 0.57 +1.2 1,051,777 + ybdK NIAGMN_05710 0.57 +0.1 1,051,893 - ybdK NIAGMN_05710 0.67 +1.9 1,051,957 - ybdK NIAGMN_05710 0.73 -2.1 1,051,978 + ybdK NIAGMN_05710 0.75 +0.5 1,052,038 + ybdK NIAGMN_05710 0.80 -0.8 1,052,109 + ybdK NIAGMN_05710 0.87 +0.1 1,052,257 + +0.2 1,052,275 - -0.7 1,052,275 - -3.0 1,052,278 + +0.7 1,052,316 - +1.6 1,052,612 - +1.5 1,052,688 - ybdF NIAGMN_05720 0.14 +0.2 1,052,837 + ybdF NIAGMN_05720 0.54 +0.6 1,052,839 + ybdF NIAGMN_05720 0.54 +0.6 1,053,059 + +1.6 1,053,059 + +1.0 1,053,080 + -2.0 1,053,244 - nfsB NIAGMN_05725 0.22 +0.2 1,053,267 + nfsB NIAGMN_05725 0.26 -1.9 1,053,298 - nfsB NIAGMN_05725 0.30 +0.4 1,053,301 + nfsB NIAGMN_05725 0.31 -2.5 1,053,301 + nfsB NIAGMN_05725 0.31 +0.7 1,053,301 + nfsB NIAGMN_05725 0.31 +0.2 1,053,302 - nfsB NIAGMN_05725 0.31 -0.0 1,053,319 - nfsB NIAGMN_05725 0.33 +0.3 1,053,323 + nfsB NIAGMN_05725 0.34 -1.0 1,053,323 + nfsB NIAGMN_05725 0.34 +1.4 1,053,646 + nfsB NIAGMN_05725 0.83 +0.4 1,053,736 - -0.7 1,053,751 + -0.2 1,053,751 + +0.3 1,053,751 + +1.1 1,053,782 - +0.4 1,053,833 + +0.2 1,053,834 - +0.9 1,053,834 - -0.3 1,053,846 + +0.8 1,053,846 + -0.2 1,053,846 + -0.1 1,053,847 - +0.3 1,053,847 - -1.0 1,053,849 - +0.3 1,053,871 + -1.2 1,053,872 - -0.5
Or see this region's nucleotide sequence