Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hokE and hokE are separated by 350 nucleotides hokE and ybdK are separated by 438 nucleotides ybdK and ybdJ are separated by 113 nucleotides ybdJ and ybdF are separated by 64 nucleotides ybdF and nfsB are separated by 93 nucleotides
NIAGMN_05690: hokE - type I toxin-antitoxin system toxin HokE, at 1,050,047 to 1,050,199
hokE
NIAGMN_05700: hokE - type I toxin-antitoxin system toxin HokE, at 1,050,550 to 1,050,702
hokE
NIAGMN_05710: ybdK - YbdK family carboxylate-amine ligase, at 1,051,141 to 1,052,259
ybdK
NIAGMN_05715: ybdJ - Uncharacterized protein YbdJ, at 1,052,373 to 1,052,573
ybdJ
NIAGMN_05720: ybdF - MmcQ/YjbR family DNA-binding protein, at 1,052,638 to 1,053,006
ybdF
NIAGMN_05725: nfsB - oxygen-insensitive NAD(P)H nitroreductase, at 1,053,100 to 1,053,753
nfsB
Position (kb)
1051
1052
1053 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1050.168 kb on - strand, within hokE at 1050.168 kb on - strand, within hokE at 1050.168 kb on - strand, within hokE at 1050.203 kb on + strand at 1050.203 kb on + strand at 1050.205 kb on + strand at 1050.275 kb on + strand at 1050.281 kb on + strand at 1050.281 kb on + strand at 1050.281 kb on + strand at 1050.282 kb on - strand at 1050.282 kb on - strand at 1050.318 kb on - strand at 1050.595 kb on - strand, within hokE at 1050.595 kb on - strand, within hokE at 1050.595 kb on - strand, within hokE at 1050.643 kb on - strand, within hokE at 1050.671 kb on - strand, within hokE at 1050.671 kb on - strand, within hokE at 1050.778 kb on - strand at 1050.784 kb on - strand at 1050.929 kb on - strand at 1051.105 kb on + strand at 1051.105 kb on + strand at 1051.106 kb on - strand at 1051.129 kb on + strand at 1051.129 kb on + strand at 1051.129 kb on + strand at 1051.130 kb on - strand at 1051.141 kb on - strand at 1051.176 kb on + strand at 1051.177 kb on - strand at 1051.222 kb on + strand at 1051.229 kb on + strand at 1051.229 kb on + strand at 1051.229 kb on + strand at 1051.230 kb on - strand at 1051.230 kb on - strand at 1051.269 kb on + strand, within ybdK at 1051.289 kb on + strand, within ybdK at 1051.290 kb on - strand, within ybdK at 1051.311 kb on + strand, within ybdK at 1051.311 kb on + strand, within ybdK at 1051.311 kb on + strand, within ybdK at 1051.312 kb on - strand, within ybdK at 1051.312 kb on - strand, within ybdK at 1051.344 kb on + strand, within ybdK at 1051.514 kb on - strand, within ybdK at 1051.648 kb on + strand, within ybdK at 1051.648 kb on + strand, within ybdK at 1051.650 kb on + strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.651 kb on - strand, within ybdK at 1051.738 kb on - strand, within ybdK at 1051.777 kb on + strand, within ybdK at 1051.777 kb on + strand, within ybdK at 1051.893 kb on - strand, within ybdK at 1051.957 kb on - strand, within ybdK at 1051.978 kb on + strand, within ybdK at 1052.038 kb on + strand, within ybdK at 1052.109 kb on + strand, within ybdK at 1052.257 kb on + strand at 1052.275 kb on - strand at 1052.275 kb on - strand at 1052.278 kb on + strand at 1052.316 kb on - strand at 1052.612 kb on - strand at 1052.688 kb on - strand, within ybdF at 1052.837 kb on + strand, within ybdF at 1052.839 kb on + strand, within ybdF at 1053.059 kb on + strand at 1053.059 kb on + strand at 1053.080 kb on + strand at 1053.244 kb on - strand, within nfsB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 1,050,168 - hokE NIAGMN_05690 0.79 -0.8 1,050,168 - hokE NIAGMN_05690 0.79 +1.1 1,050,168 - hokE NIAGMN_05690 0.79 +0.5 1,050,203 + -0.4 1,050,203 + -0.3 1,050,205 + -0.5 1,050,275 + -1.1 1,050,281 + -2.3 1,050,281 + +1.4 1,050,281 + +0.5 1,050,282 - -0.1 1,050,282 - -1.2 1,050,318 - +1.0 1,050,595 - hokE NIAGMN_05700 0.29 -2.1 1,050,595 - hokE NIAGMN_05700 0.29 -1.5 1,050,595 - hokE NIAGMN_05700 0.29 +0.7 1,050,643 - hokE NIAGMN_05700 0.61 +0.1 1,050,671 - hokE NIAGMN_05700 0.79 -0.9 1,050,671 - hokE NIAGMN_05700 0.79 -0.3 1,050,778 - +0.4 1,050,784 - +1.5 1,050,929 - +0.7 1,051,105 + +0.7 1,051,105 + -1.1 1,051,106 - +0.8 1,051,129 + -0.8 1,051,129 + +1.3 1,051,129 + +1.3 1,051,130 - +0.4 1,051,141 - +0.1 1,051,176 + +0.3 1,051,177 - -0.4 1,051,222 + -1.6 1,051,229 + -0.8 1,051,229 + +0.2 1,051,229 + +0.1 1,051,230 - -0.9 1,051,230 - -0.4 1,051,269 + ybdK NIAGMN_05710 0.11 +0.9 1,051,289 + ybdK NIAGMN_05710 0.13 -0.6 1,051,290 - ybdK NIAGMN_05710 0.13 +0.3 1,051,311 + ybdK NIAGMN_05710 0.15 +0.2 1,051,311 + ybdK NIAGMN_05710 0.15 +0.4 1,051,311 + ybdK NIAGMN_05710 0.15 -0.4 1,051,312 - ybdK NIAGMN_05710 0.15 -2.8 1,051,312 - ybdK NIAGMN_05710 0.15 -1.2 1,051,344 + ybdK NIAGMN_05710 0.18 -1.0 1,051,514 - ybdK NIAGMN_05710 0.33 +0.4 1,051,648 + ybdK NIAGMN_05710 0.45 +0.3 1,051,648 + ybdK NIAGMN_05710 0.45 -1.4 1,051,650 + ybdK NIAGMN_05710 0.45 -1.1 1,051,651 - ybdK NIAGMN_05710 0.46 -1.1 1,051,651 - ybdK NIAGMN_05710 0.46 +0.3 1,051,651 - ybdK NIAGMN_05710 0.46 +1.3 1,051,651 - ybdK NIAGMN_05710 0.46 +1.2 1,051,651 - ybdK NIAGMN_05710 0.46 -0.7 1,051,651 - ybdK NIAGMN_05710 0.46 -0.1 1,051,651 - ybdK NIAGMN_05710 0.46 +1.7 1,051,651 - ybdK NIAGMN_05710 0.46 +0.3 1,051,651 - ybdK NIAGMN_05710 0.46 +0.1 1,051,651 - ybdK NIAGMN_05710 0.46 +0.5 1,051,651 - ybdK NIAGMN_05710 0.46 +1.0 1,051,738 - ybdK NIAGMN_05710 0.53 -2.0 1,051,777 + ybdK NIAGMN_05710 0.57 +1.2 1,051,777 + ybdK NIAGMN_05710 0.57 +0.1 1,051,893 - ybdK NIAGMN_05710 0.67 +1.9 1,051,957 - ybdK NIAGMN_05710 0.73 -2.1 1,051,978 + ybdK NIAGMN_05710 0.75 +0.5 1,052,038 + ybdK NIAGMN_05710 0.80 -0.8 1,052,109 + ybdK NIAGMN_05710 0.87 +0.1 1,052,257 + +0.2 1,052,275 - -0.7 1,052,275 - -3.0 1,052,278 + +0.7 1,052,316 - +1.6 1,052,612 - +1.5 1,052,688 - ybdF NIAGMN_05720 0.14 +0.2 1,052,837 + ybdF NIAGMN_05720 0.54 +0.6 1,052,839 + ybdF NIAGMN_05720 0.54 +0.6 1,053,059 + +1.6 1,053,059 + +1.0 1,053,080 + -2.0 1,053,244 - nfsB NIAGMN_05725 0.22 +0.2
Or see this region's nucleotide sequence