Strain Fitness in Escherichia coli ECRC102 around NIAGMN_05710

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthokE and hokE are separated by 350 nucleotideshokE and ybdK are separated by 438 nucleotidesybdK and ybdJ are separated by 113 nucleotidesybdJ and ybdF are separated by 64 nucleotidesybdF and nfsB are separated by 93 nucleotides NIAGMN_05690: hokE - type I toxin-antitoxin system toxin HokE, at 1,050,047 to 1,050,199 hokE NIAGMN_05700: hokE - type I toxin-antitoxin system toxin HokE, at 1,050,550 to 1,050,702 hokE NIAGMN_05710: ybdK - YbdK family carboxylate-amine ligase, at 1,051,141 to 1,052,259 ybdK NIAGMN_05715: ybdJ - Uncharacterized protein YbdJ, at 1,052,373 to 1,052,573 ybdJ NIAGMN_05720: ybdF - MmcQ/YjbR family DNA-binding protein, at 1,052,638 to 1,053,006 ybdF NIAGMN_05725: nfsB - oxygen-insensitive NAD(P)H nitroreductase, at 1,053,100 to 1,053,753 nfsB Position (kb) 1051 1052 1053Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1050.168 kb on - strand, within hokEat 1050.168 kb on - strand, within hokEat 1050.168 kb on - strand, within hokEat 1050.203 kb on + strandat 1050.203 kb on + strandat 1050.205 kb on + strandat 1050.275 kb on + strandat 1050.281 kb on + strandat 1050.281 kb on + strandat 1050.281 kb on + strandat 1050.282 kb on - strandat 1050.282 kb on - strandat 1050.318 kb on - strandat 1050.595 kb on - strand, within hokEat 1050.595 kb on - strand, within hokEat 1050.595 kb on - strand, within hokEat 1050.643 kb on - strand, within hokEat 1050.671 kb on - strand, within hokEat 1050.671 kb on - strand, within hokEat 1050.778 kb on - strandat 1050.784 kb on - strandat 1050.929 kb on - strandat 1051.105 kb on + strandat 1051.105 kb on + strandat 1051.106 kb on - strandat 1051.129 kb on + strandat 1051.129 kb on + strandat 1051.129 kb on + strandat 1051.130 kb on - strandat 1051.141 kb on - strandat 1051.176 kb on + strandat 1051.177 kb on - strandat 1051.222 kb on + strandat 1051.229 kb on + strandat 1051.229 kb on + strandat 1051.229 kb on + strandat 1051.230 kb on - strandat 1051.230 kb on - strandat 1051.269 kb on + strand, within ybdKat 1051.289 kb on + strand, within ybdKat 1051.290 kb on - strand, within ybdKat 1051.311 kb on + strand, within ybdKat 1051.311 kb on + strand, within ybdKat 1051.311 kb on + strand, within ybdKat 1051.312 kb on - strand, within ybdKat 1051.312 kb on - strand, within ybdKat 1051.344 kb on + strand, within ybdKat 1051.514 kb on - strand, within ybdKat 1051.648 kb on + strand, within ybdKat 1051.648 kb on + strand, within ybdKat 1051.650 kb on + strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.651 kb on - strand, within ybdKat 1051.738 kb on - strand, within ybdKat 1051.777 kb on + strand, within ybdKat 1051.777 kb on + strand, within ybdKat 1051.893 kb on - strand, within ybdKat 1051.957 kb on - strand, within ybdKat 1051.978 kb on + strand, within ybdKat 1052.038 kb on + strand, within ybdKat 1052.109 kb on + strand, within ybdKat 1052.257 kb on + strandat 1052.275 kb on - strandat 1052.275 kb on - strandat 1052.278 kb on + strandat 1052.316 kb on - strandat 1052.612 kb on - strandat 1052.688 kb on - strand, within ybdFat 1052.837 kb on + strand, within ybdFat 1052.839 kb on + strand, within ybdFat 1053.059 kb on + strandat 1053.059 kb on + strandat 1053.080 kb on + strandat 1053.244 kb on - strand, within nfsB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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1,050,168 - hokE NIAGMN_05690 0.79 -0.8
1,050,168 - hokE NIAGMN_05690 0.79 +1.1
1,050,168 - hokE NIAGMN_05690 0.79 +0.5
1,050,203 + -0.4
1,050,203 + -0.3
1,050,205 + -0.5
1,050,275 + -1.1
1,050,281 + -2.3
1,050,281 + +1.4
1,050,281 + +0.5
1,050,282 - -0.1
1,050,282 - -1.2
1,050,318 - +1.0
1,050,595 - hokE NIAGMN_05700 0.29 -2.1
1,050,595 - hokE NIAGMN_05700 0.29 -1.5
1,050,595 - hokE NIAGMN_05700 0.29 +0.7
1,050,643 - hokE NIAGMN_05700 0.61 +0.1
1,050,671 - hokE NIAGMN_05700 0.79 -0.9
1,050,671 - hokE NIAGMN_05700 0.79 -0.3
1,050,778 - +0.4
1,050,784 - +1.5
1,050,929 - +0.7
1,051,105 + +0.7
1,051,105 + -1.1
1,051,106 - +0.8
1,051,129 + -0.8
1,051,129 + +1.3
1,051,129 + +1.3
1,051,130 - +0.4
1,051,141 - +0.1
1,051,176 + +0.3
1,051,177 - -0.4
1,051,222 + -1.6
1,051,229 + -0.8
1,051,229 + +0.2
1,051,229 + +0.1
1,051,230 - -0.9
1,051,230 - -0.4
1,051,269 + ybdK NIAGMN_05710 0.11 +0.9
1,051,289 + ybdK NIAGMN_05710 0.13 -0.6
1,051,290 - ybdK NIAGMN_05710 0.13 +0.3
1,051,311 + ybdK NIAGMN_05710 0.15 +0.2
1,051,311 + ybdK NIAGMN_05710 0.15 +0.4
1,051,311 + ybdK NIAGMN_05710 0.15 -0.4
1,051,312 - ybdK NIAGMN_05710 0.15 -2.8
1,051,312 - ybdK NIAGMN_05710 0.15 -1.2
1,051,344 + ybdK NIAGMN_05710 0.18 -1.0
1,051,514 - ybdK NIAGMN_05710 0.33 +0.4
1,051,648 + ybdK NIAGMN_05710 0.45 +0.3
1,051,648 + ybdK NIAGMN_05710 0.45 -1.4
1,051,650 + ybdK NIAGMN_05710 0.45 -1.1
1,051,651 - ybdK NIAGMN_05710 0.46 -1.1
1,051,651 - ybdK NIAGMN_05710 0.46 +0.3
1,051,651 - ybdK NIAGMN_05710 0.46 +1.3
1,051,651 - ybdK NIAGMN_05710 0.46 +1.2
1,051,651 - ybdK NIAGMN_05710 0.46 -0.7
1,051,651 - ybdK NIAGMN_05710 0.46 -0.1
1,051,651 - ybdK NIAGMN_05710 0.46 +1.7
1,051,651 - ybdK NIAGMN_05710 0.46 +0.3
1,051,651 - ybdK NIAGMN_05710 0.46 +0.1
1,051,651 - ybdK NIAGMN_05710 0.46 +0.5
1,051,651 - ybdK NIAGMN_05710 0.46 +1.0
1,051,738 - ybdK NIAGMN_05710 0.53 -2.0
1,051,777 + ybdK NIAGMN_05710 0.57 +1.2
1,051,777 + ybdK NIAGMN_05710 0.57 +0.1
1,051,893 - ybdK NIAGMN_05710 0.67 +1.9
1,051,957 - ybdK NIAGMN_05710 0.73 -2.1
1,051,978 + ybdK NIAGMN_05710 0.75 +0.5
1,052,038 + ybdK NIAGMN_05710 0.80 -0.8
1,052,109 + ybdK NIAGMN_05710 0.87 +0.1
1,052,257 + +0.2
1,052,275 - -0.7
1,052,275 - -3.0
1,052,278 + +0.7
1,052,316 - +1.6
1,052,612 - +1.5
1,052,688 - ybdF NIAGMN_05720 0.14 +0.2
1,052,837 + ybdF NIAGMN_05720 0.54 +0.6
1,052,839 + ybdF NIAGMN_05720 0.54 +0.6
1,053,059 + +1.6
1,053,059 + +1.0
1,053,080 + -2.0
1,053,244 - nfsB NIAGMN_05725 0.22 +0.2

Or see this region's nucleotide sequence