Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pphA and yebY are separated by 395 nucleotides yebY and copD are separated by 12 nucleotides copD and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides
NIAGMN_01635: pphA - protein-serine/threonine phosphatase, at 299,726 to 300,382
pphA
NIAGMN_01650: yebY - Uncharacterized protein YebY, at 300,778 to 301,119
yebY
NIAGMN_01655: copD - copper homeostasis membrane protein CopD, at 301,132 to 302,004
copD
NIAGMN_01660: yobA - CopC domain-containing protein YobA, at 302,008 to 302,382
yobA
NIAGMN_01665: holE - DNA polymerase III subunit theta, at 302,521 to 302,751
holE
NIAGMN_01670: yobB - Uncharacterized protein YobB, at 302,853 to 303,509
yobB
Position (kb) 301
302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 300.155 kb on + strand, within pphA at 300.476 kb on + strand at 300.478 kb on + strand at 300.478 kb on + strand at 300.516 kb on + strand at 300.597 kb on + strand at 300.609 kb on - strand at 300.775 kb on + strand at 301.204 kb on - strand at 301.269 kb on - strand, within copD at 301.296 kb on + strand, within copD at 301.296 kb on + strand, within copD at 301.297 kb on - strand, within copD at 301.297 kb on - strand, within copD at 301.325 kb on - strand, within copD at 301.348 kb on - strand, within copD at 301.456 kb on - strand, within copD at 301.491 kb on + strand, within copD at 301.491 kb on + strand, within copD at 301.491 kb on + strand, within copD at 301.492 kb on - strand, within copD at 301.511 kb on - strand, within copD at 301.636 kb on - strand, within copD at 301.645 kb on - strand, within copD at 301.645 kb on - strand, within copD at 301.662 kb on + strand, within copD at 301.696 kb on + strand, within copD at 301.929 kb on - strand at 302.027 kb on + strand at 302.028 kb on - strand at 302.028 kb on - strand at 302.081 kb on + strand, within yobA at 302.081 kb on + strand, within yobA at 302.082 kb on - strand, within yobA at 302.082 kb on - strand, within yobA at 302.164 kb on - strand, within yobA at 302.283 kb on - strand, within yobA at 302.382 kb on + strand at 302.385 kb on + strand at 302.385 kb on + strand at 302.386 kb on - strand at 302.386 kb on - strand at 302.422 kb on + strand at 302.612 kb on - strand, within holE at 302.616 kb on + strand, within holE at 302.617 kb on - strand, within holE at 302.617 kb on - strand, within holE at 302.617 kb on - strand, within holE
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 300,155 + pphA NIAGMN_01635 0.65 -0.7 300,476 + +0.9 300,478 + +0.3 300,478 + -1.3 300,516 + -0.9 300,597 + +0.9 300,609 - -0.8 300,775 + +0.7 301,204 - -1.9 301,269 - copD NIAGMN_01655 0.16 -1.1 301,296 + copD NIAGMN_01655 0.19 -0.6 301,296 + copD NIAGMN_01655 0.19 -0.1 301,297 - copD NIAGMN_01655 0.19 +1.2 301,297 - copD NIAGMN_01655 0.19 +0.8 301,325 - copD NIAGMN_01655 0.22 +0.8 301,348 - copD NIAGMN_01655 0.25 +0.3 301,456 - copD NIAGMN_01655 0.37 +0.2 301,491 + copD NIAGMN_01655 0.41 -0.2 301,491 + copD NIAGMN_01655 0.41 -3.1 301,491 + copD NIAGMN_01655 0.41 -0.0 301,492 - copD NIAGMN_01655 0.41 +0.2 301,511 - copD NIAGMN_01655 0.43 -0.5 301,636 - copD NIAGMN_01655 0.58 -0.2 301,645 - copD NIAGMN_01655 0.59 -1.8 301,645 - copD NIAGMN_01655 0.59 -1.1 301,662 + copD NIAGMN_01655 0.61 -3.1 301,696 + copD NIAGMN_01655 0.65 -0.1 301,929 - +0.5 302,027 + +0.8 302,028 - -0.2 302,028 - +0.0 302,081 + yobA NIAGMN_01660 0.19 +0.3 302,081 + yobA NIAGMN_01660 0.19 -1.8 302,082 - yobA NIAGMN_01660 0.20 +0.7 302,082 - yobA NIAGMN_01660 0.20 +0.5 302,164 - yobA NIAGMN_01660 0.42 +1.0 302,283 - yobA NIAGMN_01660 0.73 -0.1 302,382 + -0.1 302,385 + +0.8 302,385 + +1.5 302,386 - +0.2 302,386 - +0.6 302,422 + +0.1 302,612 - holE NIAGMN_01665 0.39 -0.6 302,616 + holE NIAGMN_01665 0.41 +0.8 302,617 - holE NIAGMN_01665 0.42 +1.5 302,617 - holE NIAGMN_01665 0.42 +0.5 302,617 - holE NIAGMN_01665 0.42 +2.2
Or see this region's nucleotide sequence