Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nimT and yeaO are separated by 54 nucleotides yeaO and yoaF are separated by 21 nucleotides yoaF and dgcP are separated by 182 nucleotides dgcP and yoaJ are separated by 310 nucleotides yoaJ and yeaQ are separated by 58 nucleotides yeaQ and yoaG are separated by 146 nucleotides
NIAGMN_01390: nimT - 2-nitroimidazole transporter, at 252,860 to 254,041
nimT
NIAGMN_01395: yeaO - Uncharacterized protein YeaO, at 254,096 to 254,443
yeaO
NIAGMN_01400: yoaF - DUF333 domain-containing protein, at 254,465 to 254,719
yoaF
NIAGMN_01405: dgcP - Diguanylate cyclase DgcP, at 254,902 to 255,750
dgcP
NIAGMN_01410: yoaJ - protein YoaJ, at 256,061 to 256,135
yoaJ
NIAGMN_01415: yeaQ - UPF0410 protein YeaQ, at 256,194 to 256,442
yeaQ
NIAGMN_01420: yoaG - Protein YoaG, at 256,589 to 256,771
yoaG
Position (kb) 254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 253.981 kb on - strand at 254.054 kb on + strand at 254.434 kb on + strand at 254.435 kb on - strand at 254.844 kb on - strand at 255.007 kb on - strand, within dgcP at 255.058 kb on - strand, within dgcP at 255.058 kb on - strand, within dgcP at 255.358 kb on + strand, within dgcP at 255.360 kb on + strand, within dgcP at 255.803 kb on + strand at 255.933 kb on - strand at 255.988 kb on + strand at 255.989 kb on - strand at 256.222 kb on - strand, within yeaQ at 256.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 253,981 - -1.3 254,054 + +1.4 254,434 + +0.1 254,435 - +0.2 254,844 - -1.8 255,007 - dgcP NIAGMN_01405 0.12 +2.1 255,058 - dgcP NIAGMN_01405 0.18 +1.1 255,058 - dgcP NIAGMN_01405 0.18 -0.1 255,358 + dgcP NIAGMN_01405 0.54 -0.9 255,360 + dgcP NIAGMN_01405 0.54 -3.6 255,803 + +0.9 255,933 - -0.2 255,988 + +0.5 255,989 - -0.2 256,222 - yeaQ NIAGMN_01415 0.11 -1.3 256,497 - +0.6
Or see this region's nucleotide sequence