Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01375

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdgcJ and yeaK are separated by 42 nucleotidesyeaK and yeaL are separated by 274 nucleotidesyeaL and nimR overlap by 44 nucleotides NIAGMN_01370: dgcJ - diguanylate cyclase DgcJ, at 249,228 to 250,718 dgcJ NIAGMN_01375: yeaK - mischarged aminoacyl-tRNA deacylase, at 250,761 to 251,264 yeaK NIAGMN_01380: yeaL - UPF0756 membrane protein CKO_01811, at 251,539 to 251,985 yeaL NIAGMN_01385: nimR - DNA-binding transcriptional regulator NimR, at 251,942 to 252,760 nimR Position (kb) 250 251 252Strain fitness (log2 ratio) -1 0 1at 249.907 kb on - strand, within dgcJat 249.923 kb on - strand, within dgcJat 250.660 kb on - strandat 251.017 kb on + strand, within yeaKat 251.017 kb on + strand, within yeaKat 251.027 kb on - strand, within yeaKat 251.266 kb on + strandat 251.387 kb on + strandat 251.499 kb on - strandat 251.691 kb on - strand, within yeaLat 251.984 kb on - strandat 252.038 kb on - strand, within nimRat 252.038 kb on - strand, within nimRat 252.041 kb on - strand, within nimRat 252.073 kb on + strand, within nimR

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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249,907 - dgcJ NIAGMN_01370 0.46 -1.5
249,923 - dgcJ NIAGMN_01370 0.47 +1.5
250,660 - +1.4
251,017 + yeaK NIAGMN_01375 0.51 -1.3
251,017 + yeaK NIAGMN_01375 0.51 -0.5
251,027 - yeaK NIAGMN_01375 0.53 -0.2
251,266 + +1.0
251,387 + +0.7
251,499 - -0.3
251,691 - yeaL NIAGMN_01380 0.34 +1.7
251,984 - +0.5
252,038 - nimR NIAGMN_01385 0.12 +0.4
252,038 - nimR NIAGMN_01385 0.12 -0.4
252,041 - nimR NIAGMN_01385 0.12 +0.3
252,073 + nimR NIAGMN_01385 0.16 +0.4

Or see this region's nucleotide sequence