Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00920

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfadK and ppsA are separated by 56 nucleotidesppsA and ppsR are separated by 332 nucleotides NIAGMN_00915: fadK - medium-chain fatty-acid--CoA ligase, at 158,316 to 159,962 fadK NIAGMN_00920: ppsA - phosphoenolpyruvate synthase, at 160,019 to 162,397 ppsA NIAGMN_00925: ppsR - posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR, at 162,730 to 163,563 ppsR Position (kb) 160 161 162 163Strain fitness (log2 ratio) -1 0 1at 159.097 kb on + strand, within fadKat 159.985 kb on - strandat 160.159 kb on - strandat 160.560 kb on + strand, within ppsAat 160.561 kb on - strand, within ppsAat 160.611 kb on + strand, within ppsAat 161.348 kb on - strand, within ppsAat 161.909 kb on + strand, within ppsAat 161.909 kb on + strand, within ppsAat 161.910 kb on - strand, within ppsAat 161.996 kb on - strand, within ppsAat 161.996 kb on - strand, within ppsAat 162.335 kb on + strandat 162.359 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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159,097 + fadK NIAGMN_00915 0.47 -1.2
159,985 - +0.5
160,159 - -1.0
160,560 + ppsA NIAGMN_00920 0.23 -0.7
160,561 - ppsA NIAGMN_00920 0.23 +0.8
160,611 + ppsA NIAGMN_00920 0.25 +0.4
161,348 - ppsA NIAGMN_00920 0.56 +0.4
161,909 + ppsA NIAGMN_00920 0.79 +0.3
161,909 + ppsA NIAGMN_00920 0.79 -0.4
161,910 - ppsA NIAGMN_00920 0.79 +0.8
161,996 - ppsA NIAGMN_00920 0.83 +0.2
161,996 - ppsA NIAGMN_00920 0.83 -1.2
162,335 + +0.3
162,359 - +0.7

Or see this region's nucleotide sequence