Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00465

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuidA and lacZ overlap by 7 nucleotideslacZ and uidR are separated by 390 nucleotides NIAGMN_00460: uidA - beta-glucuronidase, at 72,157 to 73,263 uidA NIAGMN_00465: lacZ - Beta-galactosidase/beta-glucuronidase, at 73,257 to 73,970 lacZ NIAGMN_00470: uidR - DNA-binding transcriptional regulator UidR, at 74,361 to 74,951 uidR Position (kb) 73 74Strain fitness (log2 ratio) -1 0 1 2at 72.372 kb on - strand, within uidAat 72.372 kb on - strand, within uidAat 73.104 kb on - strand, within uidAat 73.441 kb on + strand, within lacZat 73.588 kb on - strand, within lacZat 73.609 kb on + strand, within lacZat 73.637 kb on + strand, within lacZat 73.749 kb on - strand, within lacZat 73.787 kb on + strand, within lacZat 73.990 kb on + strandat 74.963 kb on - strandat 74.963 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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72,372 - uidA NIAGMN_00460 0.19 +0.7
72,372 - uidA NIAGMN_00460 0.19 -1.2
73,104 - uidA NIAGMN_00460 0.86 +2.5
73,441 + lacZ NIAGMN_00465 0.26 +1.4
73,588 - lacZ NIAGMN_00465 0.46 -0.9
73,609 + lacZ NIAGMN_00465 0.49 +0.8
73,637 + lacZ NIAGMN_00465 0.53 -0.0
73,749 - lacZ NIAGMN_00465 0.69 -0.2
73,787 + lacZ NIAGMN_00465 0.74 +0.6
73,990 + +1.5
74,963 - -0.6
74,963 - -0.8

Or see this region's nucleotide sequence