Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00425

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttus and fumC overlap by 4 nucleotidesfumC and fumB are separated by 142 nucleotides NIAGMN_00420: tus - DNA replication terminus site-binding protein, at 62,338 to 63,267 tus NIAGMN_00425: fumC - class II fumarate hydratase, at 63,264 to 64,667 fumC NIAGMN_00430: fumB - class I fumarate hydratase, at 64,810 to 66,456 fumB Position (kb) 63 64 65Strain fitness (log2 ratio) -2 -1 0 1 2at 62.303 kb on - strandat 62.334 kb on + strandat 62.865 kb on + strand, within tusat 62.871 kb on + strand, within tusat 63.067 kb on + strand, within tusat 63.204 kb on + strandat 63.466 kb on - strand, within fumCat 64.019 kb on - strand, within fumCat 64.656 kb on + strandat 64.661 kb on + strandat 64.812 kb on - strandat 64.844 kb on - strandat 64.844 kb on - strandat 64.966 kb on - strandat 64.966 kb on - strandat 64.966 kb on - strandat 65.165 kb on + strand, within fumBat 65.436 kb on + strand, within fumB

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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62,303 - +0.4
62,334 + +0.2
62,865 + tus NIAGMN_00420 0.57 +1.9
62,871 + tus NIAGMN_00420 0.57 +0.0
63,067 + tus NIAGMN_00420 0.78 -2.3
63,204 + -1.5
63,466 - fumC NIAGMN_00425 0.14 +0.4
64,019 - fumC NIAGMN_00425 0.54 +0.2
64,656 + -2.6
64,661 + -1.8
64,812 - -1.2
64,844 - +1.6
64,844 - -1.6
64,966 - +1.2
64,966 - -0.2
64,966 - +0.2
65,165 + fumB NIAGMN_00430 0.22 -1.4
65,436 + fumB NIAGMN_00430 0.38 -0.5

Or see this region's nucleotide sequence