Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00270

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_00260 and NIAGMN_00265 are separated by 34 nucleotidesNIAGMN_00265 and NIAGMN_00270 are separated by 500 nucleotidesNIAGMN_00270 and ynfA are separated by 205 nucleotidesynfA and ynfB are separated by 134 nucleotides NIAGMN_00260: NIAGMN_00260 - DNA-binding protein, at 32,202 to 32,438 _00260 NIAGMN_00265: NIAGMN_00265 - Integrase, cryptic prophage CP-933P, at 32,473 to 32,628 _00265 NIAGMN_00270: NIAGMN_00270 - Starvation-sensing protein, at 33,129 to 33,221 _00270 NIAGMN_00275: ynfA - YnfA family protein, at 33,427 to 33,753 ynfA NIAGMN_00280: ynfB - DUF1283 domain-containing protein, at 33,888 to 34,229 ynfB Position (kb) 33 34Strain fitness (log2 ratio) -2 -1 0 1 2at 32.319 kb on - strand, within NIAGMN_00260at 32.452 kb on + strandat 32.513 kb on + strand, within NIAGMN_00265at 32.517 kb on + strand, within NIAGMN_00265at 32.540 kb on + strand, within NIAGMN_00265at 33.138 kb on - strandat 33.141 kb on - strand, within NIAGMN_00270at 33.737 kb on - strandat 33.777 kb on + strandat 33.778 kb on - strandat 33.778 kb on - strandat 33.783 kb on + strandat 33.784 kb on - strandat 33.785 kb on + strandat 33.786 kb on - strandat 33.851 kb on + strandat 33.888 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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32,319 - NIAGMN_00260 0.49 +0.1
32,452 + -1.1
32,513 + NIAGMN_00265 0.26 +0.2
32,517 + NIAGMN_00265 0.28 -0.4
32,540 + NIAGMN_00265 0.43 -0.0
33,138 - +0.1
33,141 - NIAGMN_00270 0.13 +0.1
33,737 - -1.1
33,777 + -0.2
33,778 - +2.0
33,778 - +0.6
33,783 + -1.8
33,784 - -0.5
33,785 + -0.2
33,786 - +0.2
33,851 + +1.2
33,888 - +1.1

Or see this region's nucleotide sequence